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The map label for this gene is pgl [H]

Identifier: 116249351

GI number: 116249351

Start: 743151

End: 744515

Strand: Direct

Name: pgl [H]

Synonym: pRL120688

Alternate gene names: 116249351

Gene position: 743151-744515 (Clockwise)

Preceding gene: 116249350

Following gene: 116249352

Centisome position: 85.42

GC content: 63.74

Gene sequence:

>1365_bases
ATGAGCCCTCTATCTCTCGTCGCGATCGACCCGGCGCAGGGCGACGATACGGTGCGCCTGCAGGCGACCATCGACGGCCT
GTCGGCTTCGGGCGGCGGACGCGTGGAGCTCATGGCCGGCATCCATATCTGCCGCGGGCTCCAGCTCAGATCCGGTGTCG
ACCTTCATCTGGCCGCCGGCGCGATCCTGCGTCCCGTTCCGGACTATGCCGCCTATGCGCATACGAGTGTTTCGGTGATC
GCCGAAAAATCGGACCGCGGGATGATCGTTGCCAAGGATGCACGGCGGATCAGCCTGACGGGGGCGGGCCGCATCGAAGC
CGGTTGTGAGAGCTTCATTGTGGGAGACGACGAGACGGTGGGAACCTTCATCCCGGCCGAATTTCGGCCTCGCGTCGTCG
TCTTCGAGGGTTGCGACGAGGTCGAGATCAGCTCCGTCCATATCTGCCGGTCGCCGATGTGGACGCTGCACTTCGTCAAC
TGCACCGATGTCGCCGTCAGGAACGTGACCATCGACAACGACCGCCGCCTTCCCAACACCGACGGCATCGTGCTGGATGC
CTGCCGCGGCGCCGTCATCGAGGACTGCCGGATATCGACGGCCGACGACGGCATATGCCTGAAGACGAGCATCGGCCCTG
ATCGTGTCGCCATCGGGCGATGCGAGAATATTCTCGTCCGCCGCTGCTCCGTTCAGAGCCTCAGCTGCGCGTTGAAGATC
GGCACGGAAACGCATGGCGACGTCACCAATGTCGTGTTCGAGGATTGCAGCGTCTCATCCTCCAACAGGGCGCTGGGTGT
CTTTTCCCGCGACGGCGGCCGGATATCGAACGTAAGGTTTTCGAGAATTGCGGTAGAGTGCCGCGAGACCCCCGATGGTT
TCTGGGGCTCGGGGGAGGCGCTGACGGTCAATGTCGTCGATCGTGTCGCGGAACGCACGGCGGGCGCCATCGAAAATCTC
ATCGTCGAGGACATCACCGGTCGGATGGAGGGGGCGATCACGGTCATTTCGACCTCGTCGGCCGGCATCCGGAACGCATC
GTTGGCGCGCATCGCTATAGACCAGCAGCCGGGCCAACTCGGCACGGCGCGGTCCTACGATTTGCGTCCAACGAACGCCG
ACCTTTCCCCGAAAGCCGATGGCGGCGGCCGCGCCAATGCCTGGACCCGCGGGTCGGACGGGCGAGTGATCGGTCTTGAG
CACTATCCGGGAGGAATGCCGGCCGTCTACGTGGCTGATGTCGCCGGGATATCGATGAACGAGGTGCGGATCACGAGGCC
GACACCCCTGCCGCAAGGCTGGAACAAAAACGACGCTGTCTTCGAAACGGCGGCACCTGATGGGAGTGGGACATGGCAGA
ACTAA

Upstream 100 bases:

>100_bases
TCGATTTCTACAGGCGCATTCCGAACGTGCCGACGCCTTATGGCCAGGCGCTGACCATGCTGCTCCTGACGGAAGTTTTT
CTCGAGAACGGCAATCGCCA

Downstream 100 bases:

>100_bases
AACTTTCCAGCGTCAACAAGTCGTATGGCTCGGTCAAAGTCCTGCATGACGTCGAACTCGATATCCAAGACGGCGAGTTC
GTCGTCTTCGTCGGTCCGTC

Product: putative polygalacturonase

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSPLSLVAIDPAQGDDTVRLQATIDGLSASGGGRVELMAGIHICRGLQLRSGVDLHLAAGAILRPVPDYAAYAHTSVSVI
AEKSDRGMIVAKDARRISLTGAGRIEAGCESFIVGDDETVGTFIPAEFRPRVVVFEGCDEVEISSVHICRSPMWTLHFVN
CTDVAVRNVTIDNDRRLPNTDGIVLDACRGAVIEDCRISTADDGICLKTSIGPDRVAIGRCENILVRRCSVQSLSCALKI
GTETHGDVTNVVFEDCSVSSSNRALGVFSRDGGRISNVRFSRIAVECRETPDGFWGSGEALTVNVVDRVAERTAGAIENL
IVEDITGRMEGAITVISTSSAGIRNASLARIAIDQQPGQLGTARSYDLRPTNADLSPKADGGGRANAWTRGSDGRVIGLE
HYPGGMPAVYVADVAGISMNEVRITRPTPLPQGWNKNDAVFETAAPDGSGTWQN

Sequences:

>Translated_454_residues
MSPLSLVAIDPAQGDDTVRLQATIDGLSASGGGRVELMAGIHICRGLQLRSGVDLHLAAGAILRPVPDYAAYAHTSVSVI
AEKSDRGMIVAKDARRISLTGAGRIEAGCESFIVGDDETVGTFIPAEFRPRVVVFEGCDEVEISSVHICRSPMWTLHFVN
CTDVAVRNVTIDNDRRLPNTDGIVLDACRGAVIEDCRISTADDGICLKTSIGPDRVAIGRCENILVRRCSVQSLSCALKI
GTETHGDVTNVVFEDCSVSSSNRALGVFSRDGGRISNVRFSRIAVECRETPDGFWGSGEALTVNVVDRVAERTAGAIENL
IVEDITGRMEGAITVISTSSAGIRNASLARIAIDQQPGQLGTARSYDLRPTNADLSPKADGGGRANAWTRGSDGRVIGLE
HYPGGMPAVYVADVAGISMNEVRITRPTPLPQGWNKNDAVFETAAPDGSGTWQN
>Mature_453_residues
SPLSLVAIDPAQGDDTVRLQATIDGLSASGGGRVELMAGIHICRGLQLRSGVDLHLAAGAILRPVPDYAAYAHTSVSVIA
EKSDRGMIVAKDARRISLTGAGRIEAGCESFIVGDDETVGTFIPAEFRPRVVVFEGCDEVEISSVHICRSPMWTLHFVNC
TDVAVRNVTIDNDRRLPNTDGIVLDACRGAVIEDCRISTADDGICLKTSIGPDRVAIGRCENILVRRCSVQSLSCALKIG
TETHGDVTNVVFEDCSVSSSNRALGVFSRDGGRISNVRFSRIAVECRETPDGFWGSGEALTVNVVDRVAERTAGAIENLI
VEDITGRMEGAITVISTSSAGIRNASLARIAIDQQPGQLGTARSYDLRPTNADLSPKADGGGRANAWTRGSDGRVIGLEH
YPGGMPAVYVADVAGISMNEVRITRPTPLPQGWNKNDAVFETAAPDGSGTWQN

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 48175; Mature: 48044

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLSLVAIDPAQGDDTVRLQATIDGLSASGGGRVELMAGIHICRGLQLRSGVDLHLAAG
CCCCEEEEECCCCCCCEEEEEEEECCEECCCCCEEEEEEEHHHHCCCCCCCCCEEEEECC
AILRPVPDYAAYAHTSVSVIAEKSDRGMIVAKDARRISLTGAGRIEAGCESFIVGDDETV
HHHCCCCCHHHHHCCEEEEEEECCCCCEEEECCCEEEEEECCCCCCCCCCEEEECCCCCC
GTFIPAEFRPRVVVFEGCDEVEISSVHICRSPMWTLHFVNCTDVAVRNVTIDNDRRLPNT
CEEECCCCCCCEEEEECCCCEEEEEEEEECCCCEEEEEEEECCEEEEEEEECCCCCCCCC
DGIVLDACRGAVIEDCRISTADDGICLKTSIGPDRVAIGRCENILVRRCSVQSLSCALKI
CCEEEECCCCCEEECCEEECCCCCEEEEECCCCCCEEHHHHHHHHHHHCCCCEEEEEEEE
GTETHGDVTNVVFEDCSVSSSNRALGVFSRDGGRISNVRFSRIAVECRETPDGFWGSGEA
CCCCCCCHHEEEEECCCCCCCCCEEEEEECCCCEEEEEEEEEEEEEEECCCCCCCCCCCE
LTVNVVDRVAERTAGAIENLIVEDITGRMEGAITVISTSSAGIRNASLARIAIDQQPGQL
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCC
GTARSYDLRPTNADLSPKADGGGRANAWTRGSDGRVIGLEHYPGGMPAVYVADVAGISMN
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCCCCEEEEEEECCCEEC
EVRITRPTPLPQGWNKNDAVFETAAPDGSGTWQN
EEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
SPLSLVAIDPAQGDDTVRLQATIDGLSASGGGRVELMAGIHICRGLQLRSGVDLHLAAG
CCCEEEEECCCCCCCEEEEEEEECCEECCCCCEEEEEEEHHHHCCCCCCCCCEEEEECC
AILRPVPDYAAYAHTSVSVIAEKSDRGMIVAKDARRISLTGAGRIEAGCESFIVGDDETV
HHHCCCCCHHHHHCCEEEEEEECCCCCEEEECCCEEEEEECCCCCCCCCCEEEECCCCCC
GTFIPAEFRPRVVVFEGCDEVEISSVHICRSPMWTLHFVNCTDVAVRNVTIDNDRRLPNT
CEEECCCCCCCEEEEECCCCEEEEEEEEECCCCEEEEEEEECCEEEEEEEECCCCCCCCC
DGIVLDACRGAVIEDCRISTADDGICLKTSIGPDRVAIGRCENILVRRCSVQSLSCALKI
CCEEEECCCCCEEECCEEECCCCCEEEEECCCCCCEEHHHHHHHHHHHCCCCEEEEEEEE
GTETHGDVTNVVFEDCSVSSSNRALGVFSRDGGRISNVRFSRIAVECRETPDGFWGSGEA
CCCCCCCHHEEEEECCCCCCCCCEEEEEECCCCEEEEEEEEEEEEEEECCCCCCCCCCCE
LTVNVVDRVAERTAGAIENLIVEDITGRMEGAITVISTSSAGIRNASLARIAIDQQPGQL
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCC
GTARSYDLRPTNADLSPKADGGGRANAWTRGSDGRVIGLEHYPGGMPAVYVADVAGISMN
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCCCCEEEEEEECCCEEC
EVRITRPTPLPQGWNKNDAVFETAAPDGSGTWQN
EEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]