Click here to switch to the map view.

The map label for this gene is lacE [H]

Identifier: 116249322

GI number: 116249322

Start: 709475

End: 710773

Strand: Reverse

Name: lacE [H]

Synonym: pRL120659

Alternate gene names: 116249322

Gene position: 710773-709475 (Counterclockwise)

Preceding gene: 116249323

Following gene: 116249321

Centisome position: 81.7

GC content: 59.12

Gene sequence:

>1299_bases
ATGATCCAGGGAAGGAGAACGGGAATGTATTTGGATAAATTCGGGGGGAAGGTGAAACTTGCCGTGGCAGGATTCACCTT
GGCGGCGATGACGACCGGGGCAGCGTTTGCCCAGGACGCCGTGACGCTCAAATGGGCTTTGTGGGATTGGGACAAGACCG
CCTATTACAAGCCGCTGATCGAGGCCTATCAGGCCAAACACCCTAACGTGAAGTTCGAGCCGATGGACCTCGGCTCGCAA
GACTACCAGCAGATGATCTCGACGCAGCTGACGGGCGGCTCCAAGGACATCGACATCGTCACCATCAAGGATGTGCCGGG
CTACACCAATCTGGTGCGCGCCGGTAACATCGCCGATCTCAGCGGCTTCGTGAAGGATCAGAAGATCGACTCGGCTCCCT
TTGGCGGCCTGATCGAGGAACTGACCATCGATGGCAAGATCTACTCCCTGCCGTTCCGCTCCGACTTCTGGGTCGTCTAT
TACAACAAGGATATATTCGACAAAGCAGGCGTCGCCTACCCCACCAATGACATGACCTGGGCGCAGTTCGACGAGACCGC
CGAGAAGCTTTCGGGCGGCATGGGCACCAACAAGACCTACGGCGCGCTTCTGCATACCTGGCGGTCGACCGTTCAGCTGC
CCGGCATCCTCGACGGAAAACACACGCTTGTTGACGGCGACTATGGCTTCCTGAAGCCCTGGTACGAGAGGGCGCTGACC
CTGCAGAAGGATGGCGCAATTCCCTCCTATGCCTTCCTGAAGACGTCGAACACACATTATTCGGCGCTGTTCTTCAATGG
CACGATCGGCATGCTGCCGATGGGGACCTGGTTCGTCGGCACCCAGATCACCAAGGTGAAATCGGGTGAATCGAAGAGCA
AGAACTGGGGCATCGTCAAGTTCCCGCATCCCGACGGTGTGGCAACAGGCACGACCGCTGCGCAGATTTCCGGCCTGGCG
GTCAACGCCAATTCCGACCACAAGGATGCCGCGCTCGATTTCATCAAGTTCGTCACCGGTCCTGAGGGCGCGGCAGTCAT
CGCGTCGACGGGCACCTTCCCTGCGCTCAAGACGGATGATGTCAGCGCCAAGATCGCGGCAACACCCGGCTTTCCTGAGG
ATGCGGCCAGCATGGAGGCGCTGAAGCCGTCGAAAGCCTACCTGGAGATGGCGGTCAACCCGAACGCCGCCAAGATCGAG
GTCGTACTCAACCGGGTGCATGACGCGATCATGACCGACAGCACCTCCGTCGATGACGGGCTGAAGGAAATGACCGAAGG
CGTGAAGGCCATCAAGTAG

Upstream 100 bases:

>100_bases
GTCCATCGGGCGGCGGTGACGGTTCTAGACCTGCCAAACGCAACACAGCCGCACCGCGAACCGCATAGACGAGACGCTTC
ATAAAATAACTGATCCAGAG

Downstream 100 bases:

>100_bases
GCGTCCAGAAATGTCGACGGCCGCGGGCAATATCTGCCCGCGGCCGATCCTGTTTTTCATGAATTTCGAGCCTGACAATG
ATATATGATCCCGCCCGGGC

Product: putative substrate-binding component of ABC transporter

Products: NA

Alternate protein names: LBP [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIQGRRTGMYLDKFGGKVKLAVAGFTLAAMTTGAAFAQDAVTLKWALWDWDKTAYYKPLIEAYQAKHPNVKFEPMDLGSQ
DYQQMISTQLTGGSKDIDIVTIKDVPGYTNLVRAGNIADLSGFVKDQKIDSAPFGGLIEELTIDGKIYSLPFRSDFWVVY
YNKDIFDKAGVAYPTNDMTWAQFDETAEKLSGGMGTNKTYGALLHTWRSTVQLPGILDGKHTLVDGDYGFLKPWYERALT
LQKDGAIPSYAFLKTSNTHYSALFFNGTIGMLPMGTWFVGTQITKVKSGESKSKNWGIVKFPHPDGVATGTTAAQISGLA
VNANSDHKDAALDFIKFVTGPEGAAVIASTGTFPALKTDDVSAKIAATPGFPEDAASMEALKPSKAYLEMAVNPNAAKIE
VVLNRVHDAIMTDSTSVDDGLKEMTEGVKAIK

Sequences:

>Translated_432_residues
MIQGRRTGMYLDKFGGKVKLAVAGFTLAAMTTGAAFAQDAVTLKWALWDWDKTAYYKPLIEAYQAKHPNVKFEPMDLGSQ
DYQQMISTQLTGGSKDIDIVTIKDVPGYTNLVRAGNIADLSGFVKDQKIDSAPFGGLIEELTIDGKIYSLPFRSDFWVVY
YNKDIFDKAGVAYPTNDMTWAQFDETAEKLSGGMGTNKTYGALLHTWRSTVQLPGILDGKHTLVDGDYGFLKPWYERALT
LQKDGAIPSYAFLKTSNTHYSALFFNGTIGMLPMGTWFVGTQITKVKSGESKSKNWGIVKFPHPDGVATGTTAAQISGLA
VNANSDHKDAALDFIKFVTGPEGAAVIASTGTFPALKTDDVSAKIAATPGFPEDAASMEALKPSKAYLEMAVNPNAAKIE
VVLNRVHDAIMTDSTSVDDGLKEMTEGVKAIK
>Mature_432_residues
MIQGRRTGMYLDKFGGKVKLAVAGFTLAAMTTGAAFAQDAVTLKWALWDWDKTAYYKPLIEAYQAKHPNVKFEPMDLGSQ
DYQQMISTQLTGGSKDIDIVTIKDVPGYTNLVRAGNIADLSGFVKDQKIDSAPFGGLIEELTIDGKIYSLPFRSDFWVVY
YNKDIFDKAGVAYPTNDMTWAQFDETAEKLSGGMGTNKTYGALLHTWRSTVQLPGILDGKHTLVDGDYGFLKPWYERALT
LQKDGAIPSYAFLKTSNTHYSALFFNGTIGMLPMGTWFVGTQITKVKSGESKSKNWGIVKFPHPDGVATGTTAAQISGLA
VNANSDHKDAALDFIKFVTGPEGAAVIASTGTFPALKTDDVSAKIAATPGFPEDAASMEALKPSKAYLEMAVNPNAAKIE
VVLNRVHDAIMTDSTSVDDGLKEMTEGVKAIK

Specific function: Part of the binding-protein-dependent transport system for lactose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 46845; Mature: 46845

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQGRRTGMYLDKFGGKVKLAVAGFTLAAMTTGAAFAQDAVTLKWALWDWDKTAYYKPLI
CCCCCCCCEEHHHCCCEEEEEEECEEEEHHHCCCHHHCCCEEEEEEEECCCCCHHHHHHH
EAYQAKHPNVKFEPMDLGSQDYQQMISTQLTGGSKDIDIVTIKDVPGYTNLVRAGNIADL
HHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHCCCCCHHH
SGFVKDQKIDSAPFGGLIEELTIDGKIYSLPFRSDFWVVYYNKDIFDKAGVAYPTNDMTW
HHHHHHCCCCCCCCCCHHHHEEECCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCH
AQFDETAEKLSGGMGTNKTYGALLHTWRSTVQLPGILDGKHTLVDGDYGFLKPWYERALT
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCEECCCEEEEECCCCCCCHHHHHHEE
LQKDGAIPSYAFLKTSNTHYSALFFNGTIGMLPMGTWFVGTQITKVKSGESKSKNWGIVK
EECCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCEEEECCEEEEECCCCCCCCCCCEEE
FPHPDGVATGTTAAQISGLAVNANSDHKDAALDFIKFVTGPEGAAVIASTGTFPALKTDD
CCCCCCCCCCCHHHEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCEECCC
VSAKIAATPGFPEDAASMEALKPSKAYLEMAVNPNAAKIEVVLNRVHDAIMTDSTSVDDG
CCEEEEECCCCCCHHHHHHHCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHCCCCCHHHH
LKEMTEGVKAIK
HHHHHHHHHHCC
>Mature Secondary Structure
MIQGRRTGMYLDKFGGKVKLAVAGFTLAAMTTGAAFAQDAVTLKWALWDWDKTAYYKPLI
CCCCCCCCEEHHHCCCEEEEEEECEEEEHHHCCCHHHCCCEEEEEEEECCCCCHHHHHHH
EAYQAKHPNVKFEPMDLGSQDYQQMISTQLTGGSKDIDIVTIKDVPGYTNLVRAGNIADL
HHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHCCCCCHHH
SGFVKDQKIDSAPFGGLIEELTIDGKIYSLPFRSDFWVVYYNKDIFDKAGVAYPTNDMTW
HHHHHHCCCCCCCCCCHHHHEEECCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCH
AQFDETAEKLSGGMGTNKTYGALLHTWRSTVQLPGILDGKHTLVDGDYGFLKPWYERALT
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCEECCCEEEEECCCCCCCHHHHHHEE
LQKDGAIPSYAFLKTSNTHYSALFFNGTIGMLPMGTWFVGTQITKVKSGESKSKNWGIVK
EECCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCEEEECCEEEEECCCCCCCCCCCEEE
FPHPDGVATGTTAAQISGLAVNANSDHKDAALDFIKFVTGPEGAAVIASTGTFPALKTDD
CCCCCCCCCCCHHHEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCEECCC
VSAKIAATPGFPEDAASMEALKPSKAYLEMAVNPNAAKIEVVLNRVHDAIMTDSTSVDDG
CCEEEEECCCCCCHHHHHHHCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHCCCCCHHHH
LKEMTEGVKAIK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630315; 2318800 [H]