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The map label for this gene is bioA [C]

Identifier: 116249277

GI number: 116249277

Start: 665238

End: 666614

Strand: Reverse

Name: bioA [C]

Synonym: pRL120612

Alternate gene names: 116249277

Gene position: 666614-665238 (Counterclockwise)

Preceding gene: 116249278

Following gene: 116249276

Centisome position: 76.62

GC content: 64.27

Gene sequence:

>1377_bases
ATGTACAGCAATTCCCTCATCGAACTCGATCGCGCCCACCTCATTCATCCTGTCGCCTCCTATCGAGGCCATGAAAAGCT
TGGCGTGCGCGTGCTGGCCTCGGCCAAGGGCGCGACGGTCACCGACGCCTCCGGCAAGCAGCTGATCGACGGCTTCGCCG
GCCTCTGGTGCGTCAATGCCGGTTACGGCCACGAAAGCATCGTCGAGGCAGCAGCCCGGCAGATGCGCGAGCTTCCCTAT
GCGACGGCCTATTTCGGCCTCGGCTCAGAGCCCGCGATCCGGCTTGCCGGCGAACTCGCCGACCGCGCACCGGGCGATCT
CAACCATGTCTATTTCACGCTCGGCGGCTCCGATGCGGTGGACAGCACGATCCGCTTCATCCGCTACTACTGGCATGCCC
GTGGACGGCCCGAGCGCGATCAGTTCATCTCCGTCGAACAGGGCTATCATGGCTCCTCGACGGTCGGTGCGGGTCTGACA
GCGCTGCCTGCCTTCCATGCCGGCTTCGGCGTTCCATTCGATTGGCAGCATAAGATTCCGTCTCACTACGCCTATCGCAA
CCCGGTGGGCGACAATCCGGAGGCGATCATCAACGCCTCGCTTGCGGCGCTGAAAAGCAAGGTCGAGGCGATCGGGCCGG
AACGCGTTGCCGCTTTCTATGTCGAGCCGATCCAGGGCTCGGGCGGCGTTCTGGTTCCGCCGAAAGGCTGGATGAAAGCC
ATGCGTGAATTCTGCCGCTCGCACGACATCCTTTTTGTCGCGGACGAGGTGATTACCGGCTTTGGCCGCACCGGCCCGCT
TTTTGCCTGCACCGAGGATGAGGTCGTGCCCGATTTCATGACGACAGCCAAGGGCCTCACCTCCGGCTATGTCCCCATGG
GCGCTGTCTTGATGGCCGATCACGTCTATGAAACGATTGCCGAGGGCGCGGGTGCTGCTGCCGTCGGCCATGGCTATACC
TATTCGGCCCATCCCGTCAGCGCCGCGGTCGGCCTGGAAGTCCTGAAGCTCTACGAAAACGGCCTTCTCGAAAACGGCCT
CAGGGCCGGTGCGCGGCTGATGCAGGGCCTGGATTCTCTGAGGGATCATCCGCTCGTCGGCGATGTCCGCGGCCGCGGCA
TGCTCGCCGCCGTCGAGCTCGTGGTCGACAAGGTGAACAAAACGCCGTTGCCGGCATCTGCCGAACCCGCCCGCCGTATC
TTCGATCGCGCCTGGGAGAACGGCCTCGTCATCCGCGCCTTCGGCAATGGTGTGCTCGGCTATGCGCCGCCGCTCTGCTG
CACCGAAACGGAGATCGACGCGATCGTCGAGCGCACCCGCATGACGCTGGACGAGACGCTGGAGGACCCGGATGTGCGTC
GGGCGCTGCAGGCCTGA

Upstream 100 bases:

>100_bases
GGAATCACCATGAAAAAGCCGTTGGTTCGCGACACCGCCGATCCGACCGCCACCACCTTTGCCCTCGCCAGCCAGGCACT
CGGCTGATCCGGAGATCTCC

Downstream 100 bases:

>100_bases
GAATATCGGGAAACCGTCAGATTCGGTATTTTGTGCCGCCTCATGGCGTCTATTCGGCGAATCCGGCGCAGTGCAGGTGA
CAAACTGAAGACAACGAAAG

Product: hypothetical protein

Products: S-Adenosyl-4-methylthio-2-oxobutanoate; 7,8-Diaminononanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MYSNSLIELDRAHLIHPVASYRGHEKLGVRVLASAKGATVTDASGKQLIDGFAGLWCVNAGYGHESIVEAAARQMRELPY
ATAYFGLGSEPAIRLAGELADRAPGDLNHVYFTLGGSDAVDSTIRFIRYYWHARGRPERDQFISVEQGYHGSSTVGAGLT
ALPAFHAGFGVPFDWQHKIPSHYAYRNPVGDNPEAIINASLAALKSKVEAIGPERVAAFYVEPIQGSGGVLVPPKGWMKA
MREFCRSHDILFVADEVITGFGRTGPLFACTEDEVVPDFMTTAKGLTSGYVPMGAVLMADHVYETIAEGAGAAAVGHGYT
YSAHPVSAAVGLEVLKLYENGLLENGLRAGARLMQGLDSLRDHPLVGDVRGRGMLAAVELVVDKVNKTPLPASAEPARRI
FDRAWENGLVIRAFGNGVLGYAPPLCCTETEIDAIVERTRMTLDETLEDPDVRRALQA

Sequences:

>Translated_458_residues
MYSNSLIELDRAHLIHPVASYRGHEKLGVRVLASAKGATVTDASGKQLIDGFAGLWCVNAGYGHESIVEAAARQMRELPY
ATAYFGLGSEPAIRLAGELADRAPGDLNHVYFTLGGSDAVDSTIRFIRYYWHARGRPERDQFISVEQGYHGSSTVGAGLT
ALPAFHAGFGVPFDWQHKIPSHYAYRNPVGDNPEAIINASLAALKSKVEAIGPERVAAFYVEPIQGSGGVLVPPKGWMKA
MREFCRSHDILFVADEVITGFGRTGPLFACTEDEVVPDFMTTAKGLTSGYVPMGAVLMADHVYETIAEGAGAAAVGHGYT
YSAHPVSAAVGLEVLKLYENGLLENGLRAGARLMQGLDSLRDHPLVGDVRGRGMLAAVELVVDKVNKTPLPASAEPARRI
FDRAWENGLVIRAFGNGVLGYAPPLCCTETEIDAIVERTRMTLDETLEDPDVRRALQA
>Mature_458_residues
MYSNSLIELDRAHLIHPVASYRGHEKLGVRVLASAKGATVTDASGKQLIDGFAGLWCVNAGYGHESIVEAAARQMRELPY
ATAYFGLGSEPAIRLAGELADRAPGDLNHVYFTLGGSDAVDSTIRFIRYYWHARGRPERDQFISVEQGYHGSSTVGAGLT
ALPAFHAGFGVPFDWQHKIPSHYAYRNPVGDNPEAIINASLAALKSKVEAIGPERVAAFYVEPIQGSGGVLVPPKGWMKA
MREFCRSHDILFVADEVITGFGRTGPLFACTEDEVVPDFMTTAKGLTSGYVPMGAVLMADHVYETIAEGAGAAAVGHGYT
YSAHPVSAAVGLEVLKLYENGLLENGLRAGARLMQGLDSLRDHPLVGDVRGRGMLAAVELVVDKVNKTPLPASAEPARRI
FDRAWENGLVIRAFGNGVLGYAPPLCCTETEIDAIVERTRMTLDETLEDPDVRRALQA

Specific function: Biotin biosynthesis. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=475, Percent_Identity=28.6315789473684, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI37574042, Length=420, Percent_Identity=29.5238095238095, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI226442709, Length=387, Percent_Identity=30.2325581395349, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI226442705, Length=416, Percent_Identity=29.5673076923077, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI24119277, Length=428, Percent_Identity=28.2710280373832, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4557809, Length=417, Percent_Identity=28.2973621103118, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI284507298, Length=235, Percent_Identity=34.8936170212766, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1786991, Length=447, Percent_Identity=28.8590604026846, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789016, Length=440, Percent_Identity=30.2272727272727, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI145693181, Length=408, Percent_Identity=28.6764705882353, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1788044, Length=407, Percent_Identity=29.2383292383292, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1787560, Length=425, Percent_Identity=32.7058823529412, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789759, Length=406, Percent_Identity=28.5714285714286, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786349, Length=420, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI32564660, Length=463, Percent_Identity=30.8855291576674, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25144271, Length=409, Percent_Identity=28.361858190709, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25144274, Length=231, Percent_Identity=35.0649350649351, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71992977, Length=409, Percent_Identity=28.361858190709, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17541228, Length=480, Percent_Identity=25.4166666666667, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323470, Length=435, Percent_Identity=24.8275862068966, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324432, Length=431, Percent_Identity=24.1299303944316, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324386, Length=473, Percent_Identity=25.369978858351, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321456, Length=367, Percent_Identity=23.1607629427793, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28574759, Length=465, Percent_Identity=29.6774193548387, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI161085790, Length=462, Percent_Identity=29.8701298701299, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI21357415, Length=420, Percent_Identity=28.8095238095238, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21356575, Length=435, Percent_Identity=26.2068965517241, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI281366494, Length=383, Percent_Identity=26.8929503916449, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24667139, Length=383, Percent_Identity=26.8929503916449, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24667143, Length=383, Percent_Identity=26.8929503916449, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62 [C]

Molecular weight: Translated: 49321; Mature: 49321

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSNSLIELDRAHLIHPVASYRGHEKLGVRVLASAKGATVTDASGKQLIDGFAGLWCVNA
CCCCCHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCEEECCCCHHHHHHHHHHEEEEC
GYGHESIVEAAARQMRELPYATAYFGLGSEPAIRLAGELADRAPGDLNHVYFTLGGSDAV
CCCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHH
DSTIRFIRYYWHARGRPERDQFISVEQGYHGSSTVGAGLTALPAFHAGFGVPFDWQHKIP
HHHHHHHHHHHHCCCCCCHHHEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
SHYAYRNPVGDNPEAIINASLAALKSKVEAIGPERVAAFYVEPIQGSGGVLVPPKGWMKA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEEECCCCCEEECCHHHHHH
MREFCRSHDILFVADEVITGFGRTGPLFACTEDEVVPDFMTTAKGLTSGYVPMGAVLMAD
HHHHHHHCCEEEEEHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
HVYETIAEGAGAAAVGHGYTYSAHPVSAAVGLEVLKLYENGLLENGLRAGARLMQGLDSL
HHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDHPLVGDVRGRGMLAAVELVVDKVNKTPLPASAEPARRIFDRAWENGLVIRAFGNGVLG
HCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCC
YAPPLCCTETEIDAIVERTRMTLDETLEDPDVRRALQA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure
MYSNSLIELDRAHLIHPVASYRGHEKLGVRVLASAKGATVTDASGKQLIDGFAGLWCVNA
CCCCCHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCEEECCCCHHHHHHHHHHEEEEC
GYGHESIVEAAARQMRELPYATAYFGLGSEPAIRLAGELADRAPGDLNHVYFTLGGSDAV
CCCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHH
DSTIRFIRYYWHARGRPERDQFISVEQGYHGSSTVGAGLTALPAFHAGFGVPFDWQHKIP
HHHHHHHHHHHHCCCCCCHHHEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
SHYAYRNPVGDNPEAIINASLAALKSKVEAIGPERVAAFYVEPIQGSGGVLVPPKGWMKA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEEECCCCCEEECCHHHHHH
MREFCRSHDILFVADEVITGFGRTGPLFACTEDEVVPDFMTTAKGLTSGYVPMGAVLMAD
HHHHHHHCCEEEEEHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
HVYETIAEGAGAAAVGHGYTYSAHPVSAAVGLEVLKLYENGLLENGLRAGARLMQGLDSL
HHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDHPLVGDVRGRGMLAAVELVVDKVNKTPLPASAEPARRIFDRAWENGLVIRAFGNGVLG
HCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCC
YAPPLCCTETEIDAIVERTRMTLDETLEDPDVRRALQA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Amino-7-oxononanoate; S-Adenosyl-L-methionine [C]

Specific reaction: 8-Amino-7-oxononanoate + S-Adenosyl-L-methionine <==> S-Adenosyl-4-methylthio-2-oxobutanoate + 7,8-Diaminononanoate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]