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The map label for this gene is cfa [H]

Identifier: 116249239

GI number: 116249239

Start: 626819

End: 627847

Strand: Reverse

Name: cfa [H]

Synonym: pRL120574

Alternate gene names: 116249239

Gene position: 627847-626819 (Counterclockwise)

Preceding gene: 116249240

Following gene: 116249238

Centisome position: 72.16

GC content: 59.57

Gene sequence:

>1029_bases
ATGAATGTGATGGCTTTCGCTATCAAGACTGCCGAGCGAGCCCCGCTCAACGACAGCATGACGCTCGCCGGCATCGAGCT
CCTCTGCAACCGCACCAAGCGCAGACTGGCGGCGACGTCAGCTGAGGCCGAGAGGGTCTTTGTCGACACGATGGGGCATT
TTCCGGTCGCCACGCATACTGACCAGGCCAACCGCCAACATTATGAGGTGCCGGCCGCCTTTTTCGCGCTGGCGCTCGGG
GCGCAGACAAAATACTCATGCTGTCTATACCCGACAGATACCACGACGCTTGACGAGGCCGAAACCTTTGCTCTTGCCGA
GACCGTGAACCACGCCGCCATCGAAGATGGCATGAGTATTCTTGAGCTGGGCTGCGGCTGGGGCTCGCTGTCGCTCTATC
TCGCCACGCATTTCCCGAATTCGCGCGTCACCTCGGTATCAAACTCCGCCTCTCAGCGCGCATACATCCTTGCCCGCGCC
GAAACACTCGGATTGACCAACCTGAGCGTCATCACCGCCGACATGAACCACTTTGCCACCGACCAGCGTTATGACCGCGT
CGTCTCCGTGGAGATGTTCGAGCATATGTCGAACTGGCGGGCGCTTTTAGAGCGGGTGCACCGCTGGATCCAGCCTGACG
GCAAGCTGTTCCTGCACATCTTCACGCACAAAGACCGATCCTATCGTTTCGACCGCGCCGACCCTGCCGATTGGATCGCC
CAGCACTTCTTCACCGGCGGCATCATGCCGGCCCATGACCTGCCGCACCGATTCGGCGATCTATTCCAAGTGGAAATGGA
ATGGCGCTGGTCGGGCACGCATTATCGCCGCACTTCTCTTGACTGGCTCGCCAATTTCGATCGGTACATCGATCGCATCC
GGCCAGTCTTAAGGCAGATCTACGGCCGGGACGCATCTCTTTGGCAGCGCCGTTGGAGACTGTTCTTTCTTTCGACGGCT
GGCATGTTCGGCCATGACAGGGGCGCTATCTGGGGTGTCGGCCACTACCTTCTGGGACGAACACCATGA

Upstream 100 bases:

>100_bases
ACATGCTGCGCTCGCGCGGCGAAAAGTTCCGCGCCCTGCAGAGCCGCGTCAACGCCTTTTTCCCAGCACCTCGAAGACAG
CGCCCACAAGGAGATGAGCG

Downstream 100 bases:

>100_bases
TGGCAGTTGCACATGCCAAGCTTGATCCATTGACAACGGCGTCCGTAACGGTTGCTTGGGTCATTCTCCTGAACAAGCCG
TTTTACCCGCTCTACATTTG

Product: putative cyclopropane-fatty-acyl-phospholipid synthase

Products: NA

Alternate protein names: CFA synthase; Cyclopropane fatty acid synthase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNVMAFAIKTAERAPLNDSMTLAGIELLCNRTKRRLAATSAEAERVFVDTMGHFPVATHTDQANRQHYEVPAAFFALALG
AQTKYSCCLYPTDTTTLDEAETFALAETVNHAAIEDGMSILELGCGWGSLSLYLATHFPNSRVTSVSNSASQRAYILARA
ETLGLTNLSVITADMNHFATDQRYDRVVSVEMFEHMSNWRALLERVHRWIQPDGKLFLHIFTHKDRSYRFDRADPADWIA
QHFFTGGIMPAHDLPHRFGDLFQVEMEWRWSGTHYRRTSLDWLANFDRYIDRIRPVLRQIYGRDASLWQRRWRLFFLSTA
GMFGHDRGAIWGVGHYLLGRTP

Sequences:

>Translated_342_residues
MNVMAFAIKTAERAPLNDSMTLAGIELLCNRTKRRLAATSAEAERVFVDTMGHFPVATHTDQANRQHYEVPAAFFALALG
AQTKYSCCLYPTDTTTLDEAETFALAETVNHAAIEDGMSILELGCGWGSLSLYLATHFPNSRVTSVSNSASQRAYILARA
ETLGLTNLSVITADMNHFATDQRYDRVVSVEMFEHMSNWRALLERVHRWIQPDGKLFLHIFTHKDRSYRFDRADPADWIA
QHFFTGGIMPAHDLPHRFGDLFQVEMEWRWSGTHYRRTSLDWLANFDRYIDRIRPVLRQIYGRDASLWQRRWRLFFLSTA
GMFGHDRGAIWGVGHYLLGRTP
>Mature_342_residues
MNVMAFAIKTAERAPLNDSMTLAGIELLCNRTKRRLAATSAEAERVFVDTMGHFPVATHTDQANRQHYEVPAAFFALALG
AQTKYSCCLYPTDTTTLDEAETFALAETVNHAAIEDGMSILELGCGWGSLSLYLATHFPNSRVTSVSNSASQRAYILARA
ETLGLTNLSVITADMNHFATDQRYDRVVSVEMFEHMSNWRALLERVHRWIQPDGKLFLHIFTHKDRSYRFDRADPADWIA
QHFFTGGIMPAHDLPHRFGDLFQVEMEWRWSGTHYRRTSLDWLANFDRYIDRIRPVLRQIYGRDASLWQRRWRLFFLSTA
GMFGHDRGAIWGVGHYLLGRTP

Specific function: Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [H]

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family [H]

Homologues:

Organism=Escherichia coli, GI1787951, Length=263, Percent_Identity=28.8973384030418, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003333 [H]

Pfam domain/function: PF02353 CMAS [H]

EC number: =2.1.1.79 [H]

Molecular weight: Translated: 39198; Mature: 39198

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVMAFAIKTAERAPLNDSMTLAGIELLCNRTKRRLAATSAEAERVFVDTMGHFPVATHT
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCEECC
DQANRQHYEVPAAFFALALGAQTKYSCCLYPTDTTTLDEAETFALAETVNHAAIEDGMSI
CCCCCCHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCHHH
LELGCGWGSLSLYLATHFPNSRVTSVSNSASQRAYILARAETLGLTNLSVITADMNHFAT
HHHCCCCCCEEEEEEEECCCCCEEECCCCCCCCEEEEEEHHHCCCCEEEEEEECHHHHHC
DQRYDRVVSVEMFEHMSNWRALLERVHRWIQPDGKLFLHIFTHKDRSYRFDRADPADWIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCCCHHHHHH
QHFFTGGIMPAHDLPHRFGDLFQVEMEWRWSGTHYRRTSLDWLANFDRYIDRIRPVLRQI
HHHHCCCCCCCHHCHHHHCCEEEEEEEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHH
YGRDASLWQRRWRLFFLSTAGMFGHDRGAIWGVGHYLLGRTP
HCCCHHHHHHHHEEEEEEECCCCCCCCCCEEEHHHHHCCCCC
>Mature Secondary Structure
MNVMAFAIKTAERAPLNDSMTLAGIELLCNRTKRRLAATSAEAERVFVDTMGHFPVATHT
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCEECC
DQANRQHYEVPAAFFALALGAQTKYSCCLYPTDTTTLDEAETFALAETVNHAAIEDGMSI
CCCCCCHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCHHH
LELGCGWGSLSLYLATHFPNSRVTSVSNSASQRAYILARAETLGLTNLSVITADMNHFAT
HHHCCCCCCEEEEEEEECCCCCEEECCCCCCCCEEEEEEHHHCCCCEEEEEEECHHHHHC
DQRYDRVVSVEMFEHMSNWRALLERVHRWIQPDGKLFLHIFTHKDRSYRFDRADPADWIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCCCHHHHHH
QHFFTGGIMPAHDLPHRFGDLFQVEMEWRWSGTHYRRTSLDWLANFDRYIDRIRPVLRQI
HHHHCCCCCCCHHCHHHHCCEEEEEEEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHH
YGRDASLWQRRWRLFFLSTAGMFGHDRGAIWGVGHYLLGRTP
HCCCHHHHHHHHEEEEEEECCCCCCCCCCEEEHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]