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The map label for this gene is ttuA [H]

Identifier: 116249227

GI number: 116249227

Start: 614111

End: 615001

Strand: Direct

Name: ttuA [H]

Synonym: pRL120562

Alternate gene names: 116249227

Gene position: 614111-615001 (Clockwise)

Preceding gene: 116249224

Following gene: 116249228

Centisome position: 70.59

GC content: 61.62

Gene sequence:

>891_bases
ATGGAACTCCGTCAGCTCTCCTACTTCGTCGCGGTGGCCGAGGAACTGCACTTCGGCCGGGCGGCCGCCAAGGTGCGCAT
CGCCCAGCCGGCGCTCTCCAATCATGTGCAGGCACTGGAGCGCGAACTTGGCTGCGCGCTGTTCATCCGCTCGACCCGGC
GGGTGGAACTGACGCGGGCAGGGGAGATCTTCCACGAACGTTGCGTCGGGATCTTGAGCGAGGTCGATCTGAGTGCCGAG
ATCACCAGGGCCGTGGCCGGCAAGACGATCCGGCAGATCAGGATCGGCACCGTCTATCCGGCGACCACCGGCGTGCTGCC
GGCTTTTCTTGGAAAGATCGCCCGCAAGTATCAGGATATCCGCATCCATATATCAAGCGGCAACACTGGCGACATCATCC
GCGGCCTGGAGAACGGCCAGATCAATCTCGGCTTCATCAGACCGGTGGAAAACATCGGCTCCTTGCGCTTCTCCTCGATC
GCCCATGAGCGTTATCTGTTGGCGGTGGCCCGCAGCAACCGGCTGGCGGAACAGGCGGAGATTTCCATCGACGATCTGCG
CGGGGAAAAGATCATCGCCTTCAATCGCAAGAACCTCTCCTACACAGAACGGTACTTCAACGAGAAGTTCGAGGAATACG
ATCTGACCCGCAACATCGCCTACAGTTGCGACGACACCTATTCGCTGGTGTCGCTGGTCTCGGCCGGCCTTGGCATCGGC
TTTGCGCCGGAATGGACCGAGGGCCTGCCGAACCGGGCCTTCGAGCTGAAGGCGGTCAGGGGCATCGACTTCAGGATCGG
GCTTGGTGTCGCCTGGAATAAAGAGGATCCAACCGCCTCGCGCGACGACATCGTCGATATCGCCCGCTCATTGGCACGGC
CGGCCAGGTAA

Upstream 100 bases:

>100_bases
ATGGTTTGTCGAACGTCCATCCCATAACAAACGCATATTCACGGCTTGACAATCGACGGACTGATAGTCCCATATTAAAG
CTGCAAAAAAGGACGCTCCC

Downstream 100 bases:

>100_bases
ACGATCTGCGCTGCGGTTCAGCTGTGACGCGAGCGATGTGACGCCGCCTCGCGGATCTGCCATGTCCGCCTGCGTCTAAA
CCTCCCCTGCGATTGCGGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Tartrate utilization transcriptional regulator [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MELRQLSYFVAVAEELHFGRAAAKVRIAQPALSNHVQALERELGCALFIRSTRRVELTRAGEIFHERCVGILSEVDLSAE
ITRAVAGKTIRQIRIGTVYPATTGVLPAFLGKIARKYQDIRIHISSGNTGDIIRGLENGQINLGFIRPVENIGSLRFSSI
AHERYLLAVARSNRLAEQAEISIDDLRGEKIIAFNRKNLSYTERYFNEKFEEYDLTRNIAYSCDDTYSLVSLVSAGLGIG
FAPEWTEGLPNRAFELKAVRGIDFRIGLGVAWNKEDPTASRDDIVDIARSLARPAR

Sequences:

>Translated_296_residues
MELRQLSYFVAVAEELHFGRAAAKVRIAQPALSNHVQALERELGCALFIRSTRRVELTRAGEIFHERCVGILSEVDLSAE
ITRAVAGKTIRQIRIGTVYPATTGVLPAFLGKIARKYQDIRIHISSGNTGDIIRGLENGQINLGFIRPVENIGSLRFSSI
AHERYLLAVARSNRLAEQAEISIDDLRGEKIIAFNRKNLSYTERYFNEKFEEYDLTRNIAYSCDDTYSLVSLVSAGLGIG
FAPEWTEGLPNRAFELKAVRGIDFRIGLGVAWNKEDPTASRDDIVDIARSLARPAR
>Mature_296_residues
MELRQLSYFVAVAEELHFGRAAAKVRIAQPALSNHVQALERELGCALFIRSTRRVELTRAGEIFHERCVGILSEVDLSAE
ITRAVAGKTIRQIRIGTVYPATTGVLPAFLGKIARKYQDIRIHISSGNTGDIIRGLENGQINLGFIRPVENIGSLRFSSI
AHERYLLAVARSNRLAEQAEISIDDLRGEKIIAFNRKNLSYTERYFNEKFEEYDLTRNIAYSCDDTYSLVSLVSAGLGIG
FAPEWTEGLPNRAFELKAVRGIDFRIGLGVAWNKEDPTASRDDIVDIARSLARPAR

Specific function: Transcriptional regulator of the ttuABCDE tartrate utilization operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=244, Percent_Identity=33.6065573770492, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=27.9863481228669, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI2367136, Length=247, Percent_Identity=29.1497975708502, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787806, Length=244, Percent_Identity=29.5081967213115, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790399, Length=243, Percent_Identity=24.6913580246914, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788748, Length=248, Percent_Identity=25, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI145693105, Length=243, Percent_Identity=25.5144032921811, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081904, Length=147, Percent_Identity=30.6122448979592, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=192, Percent_Identity=27.6041666666667, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790208, Length=247, Percent_Identity=24.2914979757085, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=29.3706293706294, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790262, Length=247, Percent_Identity=23.0769230769231, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788297, Length=254, Percent_Identity=24.0157480314961, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787530, Length=249, Percent_Identity=25.3012048192771, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790783, Length=247, Percent_Identity=22.6720647773279, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=145, Percent_Identity=35.1724137931034, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33053; Mature: 33053

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRQLSYFVAVAEELHFGRAAAKVRIAQPALSNHVQALERELGCALFIRSTRRVELTRA
CCHHHHHHHHHHHHHHHCCHHHHEEEECCHHHHHHHHHHHHHHCEEEEEECCCEEEEHHH
GEIFHERCVGILSEVDLSAEITRAVAGKTIRQIRIGTVYPATTGVLPAFLGKIARKYQDI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECEECCCHHHHHHHHHHHHHHCCEEE
RIHISSGNTGDIIRGLENGQINLGFIRPVENIGSLRFSSIAHERYLLAVARSNRLAEQAE
EEEECCCCCCHHHHCCCCCCEEEEEECCHHHHCCHHHHHHHHHHHHEEEHHCCHHHHHHC
ISIDDLRGEKIIAFNRKNLSYTERYFNEKFEEYDLTRNIAYSCDDTYSLVSLVSAGLGIG
CCHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHCCCCC
FAPEWTEGLPNRAFELKAVRGIDFRIGLGVAWNKEDPTASRDDIVDIARSLARPAR
CCCCHHCCCCCCCEEEEEECCCEEEEECCEEECCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MELRQLSYFVAVAEELHFGRAAAKVRIAQPALSNHVQALERELGCALFIRSTRRVELTRA
CCHHHHHHHHHHHHHHHCCHHHHEEEECCHHHHHHHHHHHHHHCEEEEEECCCEEEEHHH
GEIFHERCVGILSEVDLSAEITRAVAGKTIRQIRIGTVYPATTGVLPAFLGKIARKYQDI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECEECCCHHHHHHHHHHHHHHCCEEE
RIHISSGNTGDIIRGLENGQINLGFIRPVENIGSLRFSSIAHERYLLAVARSNRLAEQAE
EEEECCCCCCHHHHCCCCCCEEEEEECCHHHHCCHHHHHHHHHHHHEEEHHCCHHHHHHC
ISIDDLRGEKIIAFNRKNLSYTERYFNEKFEEYDLTRNIAYSCDDTYSLVSLVSAGLGIG
CCHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHCCCCC
FAPEWTEGLPNRAFELKAVRGIDFRIGLGVAWNKEDPTASRDDIVDIARSLARPAR
CCCCHHCCCCCCCEEEEEECCCEEEEECCEEECCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592429 [H]