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The map label for this gene is putR [H]

Identifier: 116249216

GI number: 116249216

Start: 598649

End: 599143

Strand: Reverse

Name: putR [H]

Synonym: pRL120552

Alternate gene names: 116249216

Gene position: 599143-598649 (Counterclockwise)

Preceding gene: 116249225

Following gene: 116249214

Centisome position: 68.87

GC content: 55.96

Gene sequence:

>495_bases
ATGGCTACTGAAACCGACATCTTTAGTGAATTGGACCAGTTCGACAGAAAGATCCTCACCGCACTCACCGAGGACGGCAG
GCTGTCGATCACCGATCTGGCCGCGCGTGTCGGATTGTCCAAGACGCCGTGCCAGATCCGCTTCAAAAGGTTGATCAGCG
AAGGATATATCGAAGGCTTCAAGGCCGTCCTCAATCCGTCGAAAATGCAGCTCGACCATATTGCTTTCGTCGAGGTGAAG
CTTTCCGATACCCGCGAGGACGCGCTGAAAAGCTTCAACGATGCCATCAAGAAGATCAGCGAAGTCGAAGAGTGCCATAT
GATCGCCGGCAGGTTCGACTATCTCCTGAAGATCCGCACGCGCGATATCGGCCGCTACCGCCGCGTCCTCGGCGAGCGCA
TCTCGACGCTGCCCCACGTTGCCAGCACATCGACGAATGTCGCGATGGAAACGGTCAAGGAAGGCTGGGACAAGTCCGGT
TCGAGTTTTTCGTGA

Upstream 100 bases:

>100_bases
TGCCCGGCGGATAATCTATCATAATCACAAATCGCAATCTCACTTGATTTCAACAGTTTCCAGGCTGTATGAACTTATTA
ATCCGCCCGCGAGAGTTCAA

Downstream 100 bases:

>100_bases
ATGTGATCCGTGGGAGGCTATGACGAGCTGCCGGTGAGTTGCTGGAGAGAATCCAACACGCGCGCCGACCGAAGCTGCGC
ATAATCGGTCCAATTTGCCG

Product: AsnC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MATETDIFSELDQFDRKILTALTEDGRLSITDLAARVGLSKTPCQIRFKRLISEGYIEGFKAVLNPSKMQLDHIAFVEVK
LSDTREDALKSFNDAIKKISEVEECHMIAGRFDYLLKIRTRDIGRYRRVLGERISTLPHVASTSTNVAMETVKEGWDKSG
SSFS

Sequences:

>Translated_164_residues
MATETDIFSELDQFDRKILTALTEDGRLSITDLAARVGLSKTPCQIRFKRLISEGYIEGFKAVLNPSKMQLDHIAFVEVK
LSDTREDALKSFNDAIKKISEVEECHMIAGRFDYLLKIRTRDIGRYRRVLGERISTLPHVASTSTNVAMETVKEGWDKSG
SSFS
>Mature_163_residues
ATETDIFSELDQFDRKILTALTEDGRLSITDLAARVGLSKTPCQIRFKRLISEGYIEGFKAVLNPSKMQLDHIAFVEVKL
SDTREDALKSFNDAIKKISEVEECHMIAGRFDYLLKIRTRDIGRYRRVLGERISTLPHVASTSTNVAMETVKEGWDKSGS
SFS

Specific function: Transcriptional activator of the putA gene in response to proline [H]

COG id: COG1522

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH asnC-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787116, Length=145, Percent_Identity=46.2068965517241, Blast_Score=145, Evalue=1e-36,
Organism=Escherichia coli, GI87081742, Length=143, Percent_Identity=33.5664335664336, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1790182, Length=151, Percent_Identity=27.1523178807947, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011008
- InterPro:   IPR000485
- InterPro:   IPR019885
- InterPro:   IPR019888
- InterPro:   IPR019887
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01037 AsnC_trans_reg [H]

EC number: NA

Molecular weight: Translated: 18535; Mature: 18404

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS50956 HTH_ASNC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATETDIFSELDQFDRKILTALTEDGRLSITDLAARVGLSKTPCQIRFKRLISEGYIEGF
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
KAVLNPSKMQLDHIAFVEVKLSDTREDALKSFNDAIKKISEVEECHMIAGRFDYLLKIRT
HHHHCCCHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDIGRYRRVLGERISTLPHVASTSTNVAMETVKEGWDKSGSSFS
HHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ATETDIFSELDQFDRKILTALTEDGRLSITDLAARVGLSKTPCQIRFKRLISEGYIEGF
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
KAVLNPSKMQLDHIAFVEVKLSDTREDALKSFNDAIKKISEVEECHMIAGRFDYLLKIRT
HHHHCCCHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDIGRYRRVLGERISTLPHVASTSTNVAMETVKEGWDKSGSSFS
HHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606160 [H]