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The map label for this gene is yafC [H]

Identifier: 116249122

GI number: 116249122

Start: 492421

End: 493320

Strand: Direct

Name: yafC [H]

Synonym: pRL120457

Alternate gene names: 116249122

Gene position: 492421-493320 (Clockwise)

Preceding gene: 116249114

Following gene: 116249126

Centisome position: 56.6

GC content: 62.0

Gene sequence:

>900_bases
ATGAACGACTACAAGCTGCTTCGAACCTTTCTACTGGCCGCCGAGAAGCGCAACTTCGCGCAGGTCGCGCGCGAGCTCGA
CATGACCCCCGCAGCCGTCACTAGGGCAATCGCGGCCCTGGAAGAAGATCTTGGCGTGCAACTCTTTGTGCGGACCACGC
GCCAGGTATCGCTGACGACTGACGGCGCGATCTTCGCCGCACAGATCCAGCCGGCCGTGCAGACGTTGGACAATGCGCGC
AAGGATCTGATGAATACCCACAAGGCGGATCATGGCCGCCTGCGGATCAGCGCACCGACCTGGTTCGGCCAGCAGGTCCT
GCCGCCTATCCTTTCCGCATTCAGGGAACGCTACCCGAAGATCAGCTTCGAGATCTCGCTTGCCGATGGGCTCGTGAATA
TCATCGACGACGATTACGATCTGGCGATCCGCATTTCCGCAGCCCCCTCGGACAAGTTCACGATCTGGCGCAAGATCAGC
GTCGTTCACCGCATCCTCGTCGCAGCGCCCGGCAGCCGGTTTTGCGACATGAAGCACCCGAATGAACTCATTCCCGACGA
TTGCCTGGCCTATAGCGGTGACAGCAAACGGGAGACCTGGGCACTGTCGGATGGCAGTGGCAGCGCCACGATTTCTGCCG
GCCGCGCAGTCAGCGCCAATAGCGGGGAAGTGCTCGCGAGCATGGCTGCCGACGGCGCCGGGGTCGCGATGCTGCCCAAG
TTCCATGTGGCCGAGCATCTGCGGACCGGACGGCTGGCCCACGTCCTGCAGGACTGGTCGCCGCCGGACTTGTGGCTGAC
CCTGTACTATCCGCCGTATCAGGCGCTTCCGCCGCGCATCGCCTCATTCTCGAAGTTCTTCGAGCAACAGATCCCGGCGT
TTATATCGGCAATCGAATGA

Upstream 100 bases:

>100_bases
TGCGTTGGTTTGTCTGGTGTGATTGAAAACCTACGCCCGTTGATCCGCGGTCGGAATTGCAATAATAGTTGTCAGTGAAT
TGCCTCAGGTGAAATAATCA

Downstream 100 bases:

>100_bases
ACAGAGTTTGTTTTGAACAGGTCTTCGAATGGAATATTGCTCTTGACCGCATGAGCTCGCGCGAACGGCAATGCTGCGTT
GCCGGCGCAGGGATGGCGAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNDYKLLRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEEDLGVQLFVRTTRQVSLTTDGAIFAAQIQPAVQTLDNAR
KDLMNTHKADHGRLRISAPTWFGQQVLPPILSAFRERYPKISFEISLADGLVNIIDDDYDLAIRISAAPSDKFTIWRKIS
VVHRILVAAPGSRFCDMKHPNELIPDDCLAYSGDSKRETWALSDGSGSATISAGRAVSANSGEVLASMAADGAGVAMLPK
FHVAEHLRTGRLAHVLQDWSPPDLWLTLYYPPYQALPPRIASFSKFFEQQIPAFISAIE

Sequences:

>Translated_299_residues
MNDYKLLRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEEDLGVQLFVRTTRQVSLTTDGAIFAAQIQPAVQTLDNAR
KDLMNTHKADHGRLRISAPTWFGQQVLPPILSAFRERYPKISFEISLADGLVNIIDDDYDLAIRISAAPSDKFTIWRKIS
VVHRILVAAPGSRFCDMKHPNELIPDDCLAYSGDSKRETWALSDGSGSATISAGRAVSANSGEVLASMAADGAGVAMLPK
FHVAEHLRTGRLAHVLQDWSPPDLWLTLYYPPYQALPPRIASFSKFFEQQIPAFISAIE
>Mature_299_residues
MNDYKLLRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEEDLGVQLFVRTTRQVSLTTDGAIFAAQIQPAVQTLDNAR
KDLMNTHKADHGRLRISAPTWFGQQVLPPILSAFRERYPKISFEISLADGLVNIIDDDYDLAIRISAAPSDKFTIWRKIS
VVHRILVAAPGSRFCDMKHPNELIPDDCLAYSGDSKRETWALSDGSGSATISAGRAVSANSGEVLASMAADGAGVAMLPK
FHVAEHLRTGRLAHVLQDWSPPDLWLTLYYPPYQALPPRIASFSKFFEQQIPAFISAIE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=28.8194444444444, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081978, Length=305, Percent_Identity=31.4754098360656, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=28.8659793814433, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787128, Length=287, Percent_Identity=28.2229965156794, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789440, Length=281, Percent_Identity=25.9786476868327, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1787589, Length=276, Percent_Identity=24.6376811594203, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788706, Length=263, Percent_Identity=29.6577946768061, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790208, Length=252, Percent_Identity=28.968253968254, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789639, Length=259, Percent_Identity=24.7104247104247, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786448, Length=140, Percent_Identity=35, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789173, Length=284, Percent_Identity=27.112676056338, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=178, Percent_Identity=27.5280898876405, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787879, Length=305, Percent_Identity=24.5901639344262, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=295, Percent_Identity=21.6949152542373, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786713, Length=175, Percent_Identity=30.2857142857143, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=31.0344827586207, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788748, Length=119, Percent_Identity=26.890756302521, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33008; Mature: 33008

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYKLLRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEEDLGVQLFVRTTRQVSLTT
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECEEEEEEC
DGAIFAAQIQPAVQTLDNARKDLMNTHKADHGRLRISAPTWFGQQVLPPILSAFRERYPK
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
ISFEISLADGLVNIIDDDYDLAIRISAAPSDKFTIWRKISVVHRILVAAPGSRFCDMKHP
EEEEEEECCCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NELIPDDCLAYSGDSKRETWALSDGSGSATISAGRAVSANSGEVLASMAADGAGVAMLPK
CCCCCCHHHCCCCCCCCCEEEEECCCCCEEEECCCEECCCCCHHHHHHHCCCCCEEECCH
FHVAEHLRTGRLAHVLQDWSPPDLWLTLYYPPYQALPPRIASFSKFFEQQIPAFISAIE
HHHHHHHHCCHHHHHHHCCCCCCEEEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDYKLLRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEEDLGVQLFVRTTRQVSLTT
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECEEEEEEC
DGAIFAAQIQPAVQTLDNARKDLMNTHKADHGRLRISAPTWFGQQVLPPILSAFRERYPK
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
ISFEISLADGLVNIIDDDYDLAIRISAAPSDKFTIWRKISVVHRILVAAPGSRFCDMKHP
EEEEEEECCCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NELIPDDCLAYSGDSKRETWALSDGSGSATISAGRAVSANSGEVLASMAADGAGVAMLPK
CCCCCCHHHCCCCCCCCCEEEEECCCCCEEEECCCEECCCCCHHHHHHHCCCCCEEECCH
FHVAEHLRTGRLAHVLQDWSPPDLWLTLYYPPYQALPPRIASFSKFFEQQIPAFISAIE
HHHHHHHHCCHHHHHHHCCCCCCEEEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]