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The map label for this gene is siaP [H]

Identifier: 116249066

GI number: 116249066

Start: 432516

End: 433490

Strand: Direct

Name: siaP [H]

Synonym: pRL120400

Alternate gene names: 116249066

Gene position: 432516-433490 (Clockwise)

Preceding gene: 116249065

Following gene: 116249067

Centisome position: 49.71

GC content: 57.23

Gene sequence:

>975_bases
ATGAAAAGCAGACTGGCAACGATACTTTGCACCGCAGCCGTAATCATGGCGAGTAGCGCAATCGCGCAGGCCCAGACAGT
GCTGAAATGGGCGCATGTCTATGAAACGTCAGAGCCCTTCCACACGGATTCCGTATGGGCCGCAGAAGAGATCAACAAGC
GCACCGACGGGCGCTACAAGATCGAGGTCTATCCGGCATCGCAGCTCGGCAAGGAAGCCGACATCAACCAGGGCCTCAAG
CTCGGGACCGTCGATGTCATCATCTCGGGATCGAGCTTTGCGGCCCGCGACTACAAGCCGATCGGCGTGACTTACTTCCC
CTATATCTTCCGCGGTCCCGATCACCTGATCGCCTATACCAAGAGCGATGTCTTCAAACGTCTTGCCAAAGGCTATGAAG
ACAAGACCGGCAACCACATCGCCGCCGTCAGCTATTACGGCACGCGCCACACGACATCAAACAAGCCGATCGCCAAATGC
GCCGATATGCAGGGCTTGAAGATCCGCGTGCCTGACGTTCCGGCCTATCTCGCCATGCCGCGCGCATGCGGCGCCAACAC
GACGCCGATCGCCTTTGCCGAAGTCTATCTCGCTTTGCAGAACGGCACGGTCGAGGCACAGGAAAACCCGCTGACGACGA
TCGAGGCGAAGAAGTTCTACGAAGTCCAGAAGAACATCATCCTCACCGGCCACATCGTCGATCACCTCAACACCGTGATC
TCGAAGACGCGGTGGGCGAGCCTTTCCGACGAGGACAAGAAGATCTTCGGCGAGGTCATGCAGGAGGCCGCCGAGCGCAC
GAGCAAGACGATCGCCGGCAAGGAAAACGGTCTTATTGCGACCTTCAAGGAAAAAGGCTTGAACGTCGCCGAGGTCGACA
AGGCTGATTTCGAAAAGACCGTGATGGAAAAGGTCAATTTCGAGGATTTCGGCTACGAGAAGGCCGATTGGGAAGCAATC
CGCGCGATCCAGTAA

Upstream 100 bases:

>100_bases
GCTTCGGGGAGGAGTTCTCGGGGGAAGAGGAGCGCGGACGCAACAAGCGGCCGTCGAAAGGAACTGCCAAGACTTAACCC
AAGCTCGGGAGGAGAGAGCA

Downstream 100 bases:

>100_bases
AACTCGCGGCGCGGCGTCCAAGCGCGGCGCCCTTCGCTAGCAAAGCCCGCCAACTCTTCCTTCGTCATGCTCGGGCTTGT
CCCGAGCATCTGCTCCGATC

Product: putative substrate-binding component of ABC transporter

Products: NA

Alternate protein names: Extracytoplasmic solute receptor protein siaP; N-acetylneuraminic-binding protein; Neu5Ac-binding protein [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKSRLATILCTAAVIMASSAIAQAQTVLKWAHVYETSEPFHTDSVWAAEEINKRTDGRYKIEVYPASQLGKEADINQGLK
LGTVDVIISGSSFAARDYKPIGVTYFPYIFRGPDHLIAYTKSDVFKRLAKGYEDKTGNHIAAVSYYGTRHTTSNKPIAKC
ADMQGLKIRVPDVPAYLAMPRACGANTTPIAFAEVYLALQNGTVEAQENPLTTIEAKKFYEVQKNIILTGHIVDHLNTVI
SKTRWASLSDEDKKIFGEVMQEAAERTSKTIAGKENGLIATFKEKGLNVAEVDKADFEKTVMEKVNFEDFGYEKADWEAI
RAIQ

Sequences:

>Translated_324_residues
MKSRLATILCTAAVIMASSAIAQAQTVLKWAHVYETSEPFHTDSVWAAEEINKRTDGRYKIEVYPASQLGKEADINQGLK
LGTVDVIISGSSFAARDYKPIGVTYFPYIFRGPDHLIAYTKSDVFKRLAKGYEDKTGNHIAAVSYYGTRHTTSNKPIAKC
ADMQGLKIRVPDVPAYLAMPRACGANTTPIAFAEVYLALQNGTVEAQENPLTTIEAKKFYEVQKNIILTGHIVDHLNTVI
SKTRWASLSDEDKKIFGEVMQEAAERTSKTIAGKENGLIATFKEKGLNVAEVDKADFEKTVMEKVNFEDFGYEKADWEAI
RAIQ
>Mature_324_residues
MKSRLATILCTAAVIMASSAIAQAQTVLKWAHVYETSEPFHTDSVWAAEEINKRTDGRYKIEVYPASQLGKEADINQGLK
LGTVDVIISGSSFAARDYKPIGVTYFPYIFRGPDHLIAYTKSDVFKRLAKGYEDKTGNHIAAVSYYGTRHTTSNKPIAKC
ADMQGLKIRVPDVPAYLAMPRACGANTTPIAFAEVYLALQNGTVEAQENPLTTIEAKKFYEVQKNIILTGHIVDHLNTVI
SKTRWASLSDEDKKIFGEVMQEAAERTSKTIAGKENGLIATFKEKGLNVAEVDKADFEKTVMEKVNFEDFGYEKADWEAI
RAIQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system siaPT involved in the uptake of sialic acid. This protein specifically binds sialic acid [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=312, Percent_Identity=32.6923076923077, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 35931; Mature: 35931

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRLATILCTAAVIMASSAIAQAQTVLKWAHVYETSEPFHTDSVWAAEEINKRTDGRYK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEE
IEVYPASQLGKEADINQGLKLGTVDVIISGSSFAARDYKPIGVTYFPYIFRGPDHLIAYT
EEEEEHHHCCCCCCCCCCCEECEEEEEEECCCCCCCCCCCCCEEECCEEEECCCCEEEEC
KSDVFKRLAKGYEDKTGNHIAAVSYYGTRHTTSNKPIAKCADMQGLKIRVPDVPAYLAMP
HHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHCCCCCCEEECCCCCHHHHCC
RACGANTTPIAFAEVYLALQNGTVEAQENPLTTIEAKKFYEVQKNIILTGHIVDHLNTVI
CCCCCCCCCHHEEEEEEEEECCEEECCCCCCEEEHHHHHHHHHHCEEEEHHHHHHHHHHH
SKTRWASLSDEDKKIFGEVMQEAAERTSKTIAGKENGLIATFKEKGLNVAEVDKADFEKT
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCEECCCHHHHHHH
VMEKVNFEDFGYEKADWEAIRAIQ
HHHHCCHHHCCCCCCCHHHHHCCC
>Mature Secondary Structure
MKSRLATILCTAAVIMASSAIAQAQTVLKWAHVYETSEPFHTDSVWAAEEINKRTDGRYK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEE
IEVYPASQLGKEADINQGLKLGTVDVIISGSSFAARDYKPIGVTYFPYIFRGPDHLIAYT
EEEEEHHHCCCCCCCCCCCEECEEEEEEECCCCCCCCCCCCCEEECCEEEECCCCEEEEC
KSDVFKRLAKGYEDKTGNHIAAVSYYGTRHTTSNKPIAKCADMQGLKIRVPDVPAYLAMP
HHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHCCCCCCEEECCCCCHHHHCC
RACGANTTPIAFAEVYLALQNGTVEAQENPLTTIEAKKFYEVQKNIILTGHIVDHLNTVI
CCCCCCCCCHHEEEEEEEEECCEEECCCCCCEEEHHHHHHHHHHCEEEEHHHHHHHHHHH
SKTRWASLSDEDKKIFGEVMQEAAERTSKTIAGKENGLIATFKEKGLNVAEVDKADFEKT
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCEECCCHHHHHHH
VMEKVNFEDFGYEKADWEAIRAIQ
HHHHCCHHHCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA