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The map label for this gene is rbsB [H]

Identifier: 116249059

GI number: 116249059

Start: 425584

End: 426525

Strand: Reverse

Name: rbsB [H]

Synonym: pRL120393

Alternate gene names: 116249059

Gene position: 426525-425584 (Counterclockwise)

Preceding gene: 116249060

Following gene: 116249058

Centisome position: 49.02

GC content: 60.4

Gene sequence:

>942_bases
ATGAAACTGACGCGCAGACTGACACTTGCAGCTTTTGCCGGCGCATTGGCGCTCGGAACGGCTCTTCCGGCCTTTTCGGC
CGATCTCATCGCCATCATCACGCCGGCGCATGACAATCCCTTCTTCAAGGCCGAAGCCGTCGGCGCCGAAGCCAAGGCGA
AGGAGCTCGGCTACGAGACGCTGCTCATGACGCATGATGACGACGCCAACAAGCAGTCCGAAATGATCGACACGGCGATC
GGGCGCGGCGCCAAGGCGATCATCCTCGACAATGCCGGAGCTGACGCCTCCGTCGCGGCCGTCAAAAAGGCGAAGGATGC
AGGCATCCCCTCCTTCCTGATCGACCGCGAAATCAACGCGACTGGCGTTGCCGTCGCCCAGATCGTCTCGAACAACTATC
AGGGCGCCCAGCTCGGGGCGCAGGAATTCGTCAAGCTGATGGGCGAGAAGGGCAATTACGTCGAGCTGGTGGGCAAGGAG
TCCGACACCAATGCCGGCATCCGCTCGCAAGGCTACCACGACGTCATCGACGACTATCCGGATCTGAAGTCCGTCGCCAA
GCAGTCGGCCAATTGGAGCCAGACGGAAGCCTATTCGAAGATGGAAACCATCCTCCAGGCAAATCCCGACATCAAGGGCG
TCATTTCAGGGAACGACACCATGGCCATGGGTGCGATCGCAGCGCTGCAGGCTGCCGGCCGCAAGGACGTGATCGTCGTC
GGCTTCGACGGTTCCAACGACGTGCGCGACTCCATCAAGTCGGGCGGCATCAAGGCAACAGTCCTGCAGCCCGCCTATGC
GCAGGCTCAGCTGGCAGTGGAACAGGCCGACGCCTACATCAAGAACAAGACGACGCCAAAGGAAGAGAAGCAGTTGATGG
ATTGCGTTCTCATCAATGCCGACAATGCCGGCAAGCTCGAAACCTTCGCCCTGACGAACTGA

Upstream 100 bases:

>100_bases
CGAAAGCATCCGGCGCATCACCTGAACCGGCTTGGGGAGGCAAGCCCTCCCCTCAACCGCCCCAGGGAGAAGGGGCTTTA
GTCACAACAGGGAGGAAAAT

Downstream 100 bases:

>100_bases
TCAGCCGCGAATTGCGGAGGGCGCAGCACTGCGCCCTCCGCAATGCATTTCGCAAGACGTCGAGGTTCACTGCATGTTGA
GAATGAAGGGCGCCCTGCTC

Product: putative substrate-binding periplasmic protein component of ABC transporter

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKLTRRLTLAAFAGALALGTALPAFSADLIAIITPAHDNPFFKAEAVGAEAKAKELGYETLLMTHDDDANKQSEMIDTAI
GRGAKAIILDNAGADASVAAVKKAKDAGIPSFLIDREINATGVAVAQIVSNNYQGAQLGAQEFVKLMGEKGNYVELVGKE
SDTNAGIRSQGYHDVIDDYPDLKSVAKQSANWSQTEAYSKMETILQANPDIKGVISGNDTMAMGAIAALQAAGRKDVIVV
GFDGSNDVRDSIKSGGIKATVLQPAYAQAQLAVEQADAYIKNKTTPKEEKQLMDCVLINADNAGKLETFALTN

Sequences:

>Translated_313_residues
MKLTRRLTLAAFAGALALGTALPAFSADLIAIITPAHDNPFFKAEAVGAEAKAKELGYETLLMTHDDDANKQSEMIDTAI
GRGAKAIILDNAGADASVAAVKKAKDAGIPSFLIDREINATGVAVAQIVSNNYQGAQLGAQEFVKLMGEKGNYVELVGKE
SDTNAGIRSQGYHDVIDDYPDLKSVAKQSANWSQTEAYSKMETILQANPDIKGVISGNDTMAMGAIAALQAAGRKDVIVV
GFDGSNDVRDSIKSGGIKATVLQPAYAQAQLAVEQADAYIKNKTTPKEEKQLMDCVLINADNAGKLETFALTN
>Mature_313_residues
MKLTRRLTLAAFAGALALGTALPAFSADLIAIITPAHDNPFFKAEAVGAEAKAKELGYETLLMTHDDDANKQSEMIDTAI
GRGAKAIILDNAGADASVAAVKKAKDAGIPSFLIDREINATGVAVAQIVSNNYQGAQLGAQEFVKLMGEKGNYVELVGKE
SDTNAGIRSQGYHDVIDDYPDLKSVAKQSANWSQTEAYSKMETILQANPDIKGVISGNDTMAMGAIAALQAAGRKDVIVV
GFDGSNDVRDSIKSGGIKATVLQPAYAQAQLAVEQADAYIKNKTTPKEEKQLMDCVLINADNAGKLETFALTN

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=275, Percent_Identity=31.6363636363636, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790526, Length=305, Percent_Identity=28.8524590163934, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790674, Length=255, Percent_Identity=27.4509803921569, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788473, Length=300, Percent_Identity=26.3333333333333, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789990, Length=247, Percent_Identity=29.5546558704453, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33010; Mature: 33010

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTRRLTLAAFAGALALGTALPAFSADLIAIITPAHDNPFFKAEAVGAEAKAKELGYET
CCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEHHHCCCHHHHHHCCCEE
LLMTHDDDANKQSEMIDTAIGRGAKAIILDNAGADASVAAVKKAKDAGIPSFLIDREINA
EEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEECCCCCC
TGVAVAQIVSNNYQGAQLGAQEFVKLMGEKGNYVELVGKESDTNAGIRSQGYHDVIDDYP
HHHHHHHHHHCCCCCHHCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCC
DLKSVAKQSANWSQTEAYSKMETILQANPDIKGVISGNDTMAMGAIAALQAAGRKDVIVV
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEE
GFDGSNDVRDSIKSGGIKATVLQPAYAQAQLAVEQADAYIKNKTTPKEEKQLMDCVLINA
EECCCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEEC
DNAGKLETFALTN
CCCCCEEEEEECC
>Mature Secondary Structure
MKLTRRLTLAAFAGALALGTALPAFSADLIAIITPAHDNPFFKAEAVGAEAKAKELGYET
CCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEHHHCCCHHHHHHCCCEE
LLMTHDDDANKQSEMIDTAIGRGAKAIILDNAGADASVAAVKKAKDAGIPSFLIDREINA
EEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEECCCCCC
TGVAVAQIVSNNYQGAQLGAQEFVKLMGEKGNYVELVGKESDTNAGIRSQGYHDVIDDYP
HHHHHHHHHHCCCCCHHCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCC
DLKSVAKQSANWSQTEAYSKMETILQANPDIKGVISGNDTMAMGAIAALQAAGRKDVIVV
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEE
GFDGSNDVRDSIKSGGIKATVLQPAYAQAQLAVEQADAYIKNKTTPKEEKQLMDCVLINA
EECCCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEEC
DNAGKLETFALTN
CCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]