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The map label for this gene is panB [H]

Identifier: 116249029

GI number: 116249029

Start: 389251

End: 390072

Strand: Direct

Name: panB [H]

Synonym: pRL120360

Alternate gene names: 116249029

Gene position: 389251-390072 (Clockwise)

Preceding gene: 116249028

Following gene: 116249031

Centisome position: 44.74

GC content: 63.02

Gene sequence:

>822_bases
ATGAGCGCGGCCGGAATTCAGAAGCGCATTACGCCAGCGCGCATCTCAGCCATGAAGGGCGGCAAGCCGATCGTCTGCCT
GACCGCCTATACGACGCCGATGGCGCGGCTGCTCGATGAGCATTGCGACCTCCTGCTGGTCGGGGATTCGCTCGGCATGG
TGCTCTACGGTATGGAGACCACCATCGGCGTCACGCTCGATATGATGATCGCACACGGCAAGGCGGTGATGCGCGGCGTC
GGTAAGGCCTGCGTCGTCGTCGACATGCCGTTCGGCAGTTATCAGGAATCGAAGGAGATTGCCTTCCGCAATGCTGTCCG
CATCCTGCAGGAAACCGGCTGCGATGCCGTAAAGCTGGAGGGCGGCGAGGAGATGGCCGAGACCATCGCCTTTCTGACGA
AGCGGGGGATTCCGGTTATGGGCCATATCGGCCTGATGCCGCAGCAGGTCCAAACCGCAGGCGGTTATCGATCTGTCGGC
CATTCCGAACACGAGACGTCGAAGATCCGCCGCGATGCCCACGCCGTTGGCGGCTCGGGCGCCTTTGCCGTCGTCATCGA
GGGCACGGTGGAGCCGCTCGCCCGGGAGGTGACGTCCGCCGTGCACGTCCCGACCATCGGCATCGGCGCTTCTTCCGCCT
GCGACGGTCAGGTTCTGGTTTCCGACGACATTCTGGGCCTCTTCAACGATTTCACCCCGCGCTTCGTGAAGCGCTACGAC
GAACTCGGCAAGAGGATTTCAGCCGCCGCTGCCGCTTATGCCGACGAGGTCCGATCGCGACAGTTTCCGGCAGCGGAGCA
TACGTTCAAACGGCGATCTTGA

Upstream 100 bases:

>100_bases
CGACACGGCTTCTCGACAACAGGGTCGTTGGGAGCAACCGGTTTGTCGGGAACAACAGGGTTGCCGGCGGGCAGAGCTTG
CCGGGAAAGGGAGTGACCAG

Downstream 100 bases:

>100_bases
GGCGAGGCCATATCAGATCCATCACCGGGCGGCATACTGTTCGCGCATGCTGTCGGCCGACCGGCGAACCGCCTCGGAAA
CCTGCTGGAGCTGTTTGGCC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSAAGIQKRITPARISAMKGGKPIVCLTAYTTPMARLLDEHCDLLLVGDSLGMVLYGMETTIGVTLDMMIAHGKAVMRGV
GKACVVVDMPFGSYQESKEIAFRNAVRILQETGCDAVKLEGGEEMAETIAFLTKRGIPVMGHIGLMPQQVQTAGGYRSVG
HSEHETSKIRRDAHAVGGSGAFAVVIEGTVEPLAREVTSAVHVPTIGIGASSACDGQVLVSDDILGLFNDFTPRFVKRYD
ELGKRISAAAAAYADEVRSRQFPAAEHTFKRRS

Sequences:

>Translated_273_residues
MSAAGIQKRITPARISAMKGGKPIVCLTAYTTPMARLLDEHCDLLLVGDSLGMVLYGMETTIGVTLDMMIAHGKAVMRGV
GKACVVVDMPFGSYQESKEIAFRNAVRILQETGCDAVKLEGGEEMAETIAFLTKRGIPVMGHIGLMPQQVQTAGGYRSVG
HSEHETSKIRRDAHAVGGSGAFAVVIEGTVEPLAREVTSAVHVPTIGIGASSACDGQVLVSDDILGLFNDFTPRFVKRYD
ELGKRISAAAAAYADEVRSRQFPAAEHTFKRRS
>Mature_272_residues
SAAGIQKRITPARISAMKGGKPIVCLTAYTTPMARLLDEHCDLLLVGDSLGMVLYGMETTIGVTLDMMIAHGKAVMRGVG
KACVVVDMPFGSYQESKEIAFRNAVRILQETGCDAVKLEGGEEMAETIAFLTKRGIPVMGHIGLMPQQVQTAGGYRSVGH
SEHETSKIRRDAHAVGGSGAFAVVIEGTVEPLAREVTSAVHVPTIGIGASSACDGQVLVSDDILGLFNDFTPRFVKRYDE
LGKRISAAAAAYADEVRSRQFPAAEHTFKRRS

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=249, Percent_Identity=38.9558232931727, Blast_Score=172, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319653, Length=261, Percent_Identity=37.1647509578544, Blast_Score=162, Evalue=8e-41,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 29183; Mature: 29052

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAGIQKRITPARISAMKGGKPIVCLTAYTTPMARLLDEHCDLLLVGDSLGMVLYGMET
CCCCCCHHCCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCCEEEECCCCCEEEEECHH
TIGVTLDMMIAHGKAVMRGVGKACVVVDMPFGSYQESKEIAFRNAVRILQETGCDAVKLE
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
GGEEMAETIAFLTKRGIPVMGHIGLMPQQVQTAGGYRSVGHSEHETSKIRRDAHAVGGSG
CCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
AFAVVIEGTVEPLAREVTSAVHVPTIGIGASSACDGQVLVSDDILGLFNDFTPRFVKRYD
EEEEEEECCHHHHHHHHHHHHCCCEECCCCCCCCCCEEEECCCHHHHHHCCCHHHHHHHH
ELGKRISAAAAAYADEVRSRQFPAAEHTFKRRS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC
>Mature Secondary Structure 
SAAGIQKRITPARISAMKGGKPIVCLTAYTTPMARLLDEHCDLLLVGDSLGMVLYGMET
CCCCCHHCCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCCEEEECCCCCEEEEECHH
TIGVTLDMMIAHGKAVMRGVGKACVVVDMPFGSYQESKEIAFRNAVRILQETGCDAVKLE
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
GGEEMAETIAFLTKRGIPVMGHIGLMPQQVQTAGGYRSVGHSEHETSKIRRDAHAVGGSG
CCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
AFAVVIEGTVEPLAREVTSAVHVPTIGIGASSACDGQVLVSDDILGLFNDFTPRFVKRYD
EEEEEEECCHHHHHHHHHHHHCCCEECCCCCCCCCCEEEECCCHHHHHHCCCHHHHHHHH
ELGKRISAAAAAYADEVRSRQFPAAEHTFKRRS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA