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The map label for this gene is yafC [H]

Identifier: 116249016

GI number: 116249016

Start: 372947

End: 373888

Strand: Direct

Name: yafC [H]

Synonym: pRL120347

Alternate gene names: 116249016

Gene position: 372947-373888 (Clockwise)

Preceding gene: 116249014

Following gene: 116249018

Centisome position: 42.87

GC content: 59.66

Gene sequence:

>942_bases
GTGGACAGTCTCGCAAGTGTTGTCGCCTTCGTTCACGCCGCCGAACAGCAGAGTTATGTCGCAGCCGCCAGGGTTGGCGG
AGTTTCGCCGTCCGCGATCGGAAAGGCAGTGGCGCGTCTCGAAGGCCGGCTGGGCGTGCGGCTCTTCAACCGGACGACGC
GCAGTATCAGCCTGACTGAGGAAGGAGCGGTCCTCTACGAGCGCTACAAACGCATCATCGATGACATGGACGACGCAGAG
GCCACAATCTCACGAAGCCGGGAACGACCAAGGGGGCGCCTTCGCGTCAGCGTACCGCATATCGTCGGACACCACCTTCT
TATGCCGATCCTGCCCATTTTTGCCGAGCAGTTTCCGGAGATCGAACTGGACATCGATTTCGAAGACAAGGTCACCGATC
TGGTTGCAGAGGGCCTGGATGTCGTCGTGCGCAGTGGTGAGCTTGCCGATGCACGATTGATTGCCCGCCGTCTCGGCGAT
CAGCATTTCGTCGTTTGCGGAAGTCCCGATTATCTCAAGCGCCATGGGCGACCGGAGACCCCGGATGACCTCTCCCAACA
TGCCTGCATTCACTTCAAATACCCATCCAGCGGCCGGCTCGCACCATGGGCATTCCGCTCACCATACGATCGGGTAATTT
TTCCAAGAACCCTTACATTCAACAATACGGACGCAGGGCTGCGGGCAGCACGAGACGGTTTGGGGATCGCGCATCTGCCT
GTCTACGTCGCAGAGCCCCATATGCGAACCGGGAGTCTGGTCCCCGTCCTGACCTCCTTCATGGTGCCGTACGGCTCGCT
CTCGCTCGTCTGGCCGTCCAATCGTCAGCTTTCACCAAAAGTCCGCGCTTTCGTGGATTTTGTGGCTGCGAACTTTGCCG
CCCGACCCGACGCTTTTCGCCCGCTGGATGCGGCTGGCATCCCATCCAATTCTAGGAGATAG

Upstream 100 bases:

>100_bases
TGGCTGCGCATCGAGACAGAGGATATTTCCTATGCAATATTGATCAATTACGGCTCTTGGACTTACATAAAGGACAAAAT
ATCTTCAATCGGGTGCTGAT

Downstream 100 bases:

>100_bases
CAGGCGCTTTCAGGAGCGCCCGCCAGTTCTTGAAATCATGGTGCGACATGCTGCCGTAGGCCATTTGATTCCCGGCACGT
ATGGCGGTCAGGCGTCAGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MDSLASVVAFVHAAEQQSYVAAARVGGVSPSAIGKAVARLEGRLGVRLFNRTTRSISLTEEGAVLYERYKRIIDDMDDAE
ATISRSRERPRGRLRVSVPHIVGHHLLMPILPIFAEQFPEIELDIDFEDKVTDLVAEGLDVVVRSGELADARLIARRLGD
QHFVVCGSPDYLKRHGRPETPDDLSQHACIHFKYPSSGRLAPWAFRSPYDRVIFPRTLTFNNTDAGLRAARDGLGIAHLP
VYVAEPHMRTGSLVPVLTSFMVPYGSLSLVWPSNRQLSPKVRAFVDFVAANFAARPDAFRPLDAAGIPSNSRR

Sequences:

>Translated_313_residues
MDSLASVVAFVHAAEQQSYVAAARVGGVSPSAIGKAVARLEGRLGVRLFNRTTRSISLTEEGAVLYERYKRIIDDMDDAE
ATISRSRERPRGRLRVSVPHIVGHHLLMPILPIFAEQFPEIELDIDFEDKVTDLVAEGLDVVVRSGELADARLIARRLGD
QHFVVCGSPDYLKRHGRPETPDDLSQHACIHFKYPSSGRLAPWAFRSPYDRVIFPRTLTFNNTDAGLRAARDGLGIAHLP
VYVAEPHMRTGSLVPVLTSFMVPYGSLSLVWPSNRQLSPKVRAFVDFVAANFAARPDAFRPLDAAGIPSNSRR
>Mature_313_residues
MDSLASVVAFVHAAEQQSYVAAARVGGVSPSAIGKAVARLEGRLGVRLFNRTTRSISLTEEGAVLYERYKRIIDDMDDAE
ATISRSRERPRGRLRVSVPHIVGHHLLMPILPIFAEQFPEIELDIDFEDKVTDLVAEGLDVVVRSGELADARLIARRLGD
QHFVVCGSPDYLKRHGRPETPDDLSQHACIHFKYPSSGRLAPWAFRSPYDRVIFPRTLTFNNTDAGLRAARDGLGIAHLP
VYVAEPHMRTGSLVPVLTSFMVPYGSLSLVWPSNRQLSPKVRAFVDFVAANFAARPDAFRPLDAAGIPSNSRR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=36.8055555555556, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=32.9931972789116, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1787128, Length=300, Percent_Identity=31, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=32.9861111111111, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI87081978, Length=288, Percent_Identity=32.9861111111111, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1789639, Length=306, Percent_Identity=28.1045751633987, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789440, Length=304, Percent_Identity=26.6447368421053, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=26.9360269360269, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=23.3108108108108, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786448, Length=284, Percent_Identity=26.7605633802817, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790011, Length=269, Percent_Identity=23.7918215613383, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=23.448275862069, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=243, Percent_Identity=27.1604938271605, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI157672245, Length=135, Percent_Identity=29.6296296296296, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34618; Mature: 34618

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSLASVVAFVHAAEQQSYVAAARVGGVSPSAIGKAVARLEGRLGVRLFNRTTRSISLTE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEECC
EGAVLYERYKRIIDDMDDAEATISRSRERPRGRLRVSVPHIVGHHLLMPILPIFAEQFPE
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCC
IELDIDFEDKVTDLVAEGLDVVVRSGELADARLIARRLGDQHFVVCGSPDYLKRHGRPET
EEEEECCHHHHHHHHHHCHHHEEECCCHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCC
PDDLSQHACIHFKYPSSGRLAPWAFRSPYDRVIFPRTLTFNNTDAGLRAARDGLGIAHLP
CHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEECEEEEECCCCHHHHHHHCCCCEEECE
VYVAEPHMRTGSLVPVLTSFMVPYGSLSLVWPSNRQLSPKVRAFVDFVAANFAARPDAFR
EEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCC
PLDAAGIPSNSRR
CCCCCCCCCCCCC
>Mature Secondary Structure
MDSLASVVAFVHAAEQQSYVAAARVGGVSPSAIGKAVARLEGRLGVRLFNRTTRSISLTE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEECC
EGAVLYERYKRIIDDMDDAEATISRSRERPRGRLRVSVPHIVGHHLLMPILPIFAEQFPE
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCC
IELDIDFEDKVTDLVAEGLDVVVRSGELADARLIARRLGDQHFVVCGSPDYLKRHGRPET
EEEEECCHHHHHHHHHHCHHHEEECCCHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCC
PDDLSQHACIHFKYPSSGRLAPWAFRSPYDRVIFPRTLTFNNTDAGLRAARDGLGIAHLP
CHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEECEEEEECCCCHHHHHHHCCCCEEECE
VYVAEPHMRTGSLVPVLTSFMVPYGSLSLVWPSNRQLSPKVRAFVDFVAANFAARPDAFR
EEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCC
PLDAAGIPSNSRR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]