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The map label for this gene is rbsB [H]

Identifier: 116248870

GI number: 116248870

Start: 208183

End: 209130

Strand: Reverse

Name: rbsB [H]

Synonym: pRL120200

Alternate gene names: 116248870

Gene position: 209130-208183 (Counterclockwise)

Preceding gene: 116248871

Following gene: 116248869

Centisome position: 24.04

GC content: 58.97

Gene sequence:

>948_bases
ATGTTTAAGAAAGGCATGCGCATACTTCTGGCCGCCGCGGCCGTAGCACCGCTTCTTGCAAGCTCGGCTTGGGCAGCGGG
CATGATGACGATCATCGTCAACGATCCGTCGAATCCTTATTGGCTGACGGAAGGCAATGTCGCCAAGGCAACTGCCGAAA
AGCTGGGCTACACGGCCTCGGTCAACGCCCACAAGGGTGACACCAATACCGAAAGCAACCTGATCGATACGGCGATCACC
AACAAGTCGGTGGCGATCATCCTCGACCCGGCAAACGCCGACGGTTCTGTCGGTGCGGTCAAGAAGGCCGTTGCTGCCGG
CATCCCGGTCATCCTCGTCAACGCCGAAATCAACCAGGAAGGCCTGGCCAAGGCACAGCTTGTTTCCAACAACGCACAGG
GCGCCGCTCTCGGCGCACAGCAATGGGTCGAAGCGATCGGCGACAAGGGCAACTATGCCGAACTCTTCGGCGCTCCCTCC
GACAACAACGCGGCAACGCGGTCGAATGGCTACGAGACTGTCCTTTCGCAGTATCCGGATCTGAAGAAGGTCGCCAAGGA
AGTTGCCAACTGGGACCGCACTCAGGGCCACAACAAGATGCAGTCCATGCTGCAGGCCAACCCGGATATCGTCGGCGTCA
TCTCGGGCAATGACGAAATGGCGCTCGGTGCGATCGCCGCACTCAAGGAAGCCGGCAAGCTCGCCAATGTGAAGGTCGGC
GGCTTCGACGGTTCGCCGGATGCGGTTGCGGCCATCAAGGCGGGCGAACTCCAATACACCGTCCTCCAACCGGTTGCCGT
CTTCTCCGAAGAAGCCGTCAAACAGGCCGACAATCTGATCAAGACAGGTAACACGGGTGCCAAGAGCGAAAAGCAGCTCT
TCGATTGCCTGTTGATCACCAAGGACAACATCGACAAGTACACCGGTCCGTTCGTTCTGGCGCAGTAA

Upstream 100 bases:

>100_bases
GAAACTTATCCACGACGGCAGGCTGGAGTCCAGAAAGCCGATTGGGGAAAGCAACGCCGGATCACCGGCAATAACTGAGC
TCACGAATGGGAGAGAGACT

Downstream 100 bases:

>100_bases
ACGGTAAAAGGGCGCTCGCAAGAGCGCCCTCATTTCATGCTGGAAGCGATGGCCGACGGCCCAACCGCCGGTTGCGCTTC
GCCTTTTTGCCTTGGCGCGG

Product: putative substrate binding component of ABC transporter

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MFKKGMRILLAAAAVAPLLASSAWAAGMMTIIVNDPSNPYWLTEGNVAKATAEKLGYTASVNAHKGDTNTESNLIDTAIT
NKSVAIILDPANADGSVGAVKKAVAAGIPVILVNAEINQEGLAKAQLVSNNAQGAALGAQQWVEAIGDKGNYAELFGAPS
DNNAATRSNGYETVLSQYPDLKKVAKEVANWDRTQGHNKMQSMLQANPDIVGVISGNDEMALGAIAALKEAGKLANVKVG
GFDGSPDAVAAIKAGELQYTVLQPVAVFSEEAVKQADNLIKTGNTGAKSEKQLFDCLLITKDNIDKYTGPFVLAQ

Sequences:

>Translated_315_residues
MFKKGMRILLAAAAVAPLLASSAWAAGMMTIIVNDPSNPYWLTEGNVAKATAEKLGYTASVNAHKGDTNTESNLIDTAIT
NKSVAIILDPANADGSVGAVKKAVAAGIPVILVNAEINQEGLAKAQLVSNNAQGAALGAQQWVEAIGDKGNYAELFGAPS
DNNAATRSNGYETVLSQYPDLKKVAKEVANWDRTQGHNKMQSMLQANPDIVGVISGNDEMALGAIAALKEAGKLANVKVG
GFDGSPDAVAAIKAGELQYTVLQPVAVFSEEAVKQADNLIKTGNTGAKSEKQLFDCLLITKDNIDKYTGPFVLAQ
>Mature_315_residues
MFKKGMRILLAAAAVAPLLASSAWAAGMMTIIVNDPSNPYWLTEGNVAKATAEKLGYTASVNAHKGDTNTESNLIDTAIT
NKSVAIILDPANADGSVGAVKKAVAAGIPVILVNAEINQEGLAKAQLVSNNAQGAALGAQQWVEAIGDKGNYAELFGAPS
DNNAATRSNGYETVLSQYPDLKKVAKEVANWDRTQGHNKMQSMLQANPDIVGVISGNDEMALGAIAALKEAGKLANVKVG
GFDGSPDAVAAIKAGELQYTVLQPVAVFSEEAVKQADNLIKTGNTGAKSEKQLFDCLLITKDNIDKYTGPFVLAQ

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=289, Percent_Identity=30.1038062283737, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1790526, Length=299, Percent_Identity=27.0903010033445, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1790674, Length=226, Percent_Identity=28.7610619469027, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789990, Length=245, Percent_Identity=27.3469387755102, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32828; Mature: 32828

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKGMRILLAAAAVAPLLASSAWAAGMMTIIVNDPSNPYWLTEGNVAKATAEKLGYTAS
CCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCEEEECCCCHHHHHHHCCCEEE
VNAHKGDTNTESNLIDTAITNKSVAIILDPANADGSVGAVKKAVAAGIPVILVNAEINQE
CCCCCCCCCCCCHHHHHEECCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCC
GLAKAQLVSNNAQGAALGAQQWVEAIGDKGNYAELFGAPSDNNAATRSNGYETVLSQYPD
HHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCC
LKKVAKEVANWDRTQGHNKMQSMLQANPDIVGVISGNDEMALGAIAALKEAGKLANVKVG
HHHHHHHHHCCHHCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEC
GFDGSPDAVAAIKAGELQYTVLQPVAVFSEEAVKQADNLIKTGNTGAKSEKQLFDCLLIT
CCCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEE
KDNIDKYTGPFVLAQ
CCCCCCCCCCEEEEC
>Mature Secondary Structure
MFKKGMRILLAAAAVAPLLASSAWAAGMMTIIVNDPSNPYWLTEGNVAKATAEKLGYTAS
CCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCEEEECCCCHHHHHHHCCCEEE
VNAHKGDTNTESNLIDTAITNKSVAIILDPANADGSVGAVKKAVAAGIPVILVNAEINQE
CCCCCCCCCCCCHHHHHEECCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCC
GLAKAQLVSNNAQGAALGAQQWVEAIGDKGNYAELFGAPSDNNAATRSNGYETVLSQYPD
HHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCC
LKKVAKEVANWDRTQGHNKMQSMLQANPDIVGVISGNDEMALGAIAALKEAGKLANVKVG
HHHHHHHHHCCHHCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEC
GFDGSPDAVAAIKAGELQYTVLQPVAVFSEEAVKQADNLIKTGNTGAKSEKQLFDCLLIT
CCCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEE
KDNIDKYTGPFVLAQ
CCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]