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The map label for this gene is potB [H]

Identifier: 116248820

GI number: 116248820

Start: 146589

End: 147488

Strand: Reverse

Name: potB [H]

Synonym: pRL120146

Alternate gene names: 116248820

Gene position: 147488-146589 (Counterclockwise)

Preceding gene: 116248821

Following gene: 116248819

Centisome position: 16.95

GC content: 60.0

Gene sequence:

>900_bases
ATGTCGATAAGTGCCACAACAGCCGAGACCGAGACCACCGTGGAACCGGTCAGGAAGGCATCCGGCAAGACGCTCTGGCT
GTTGGCCCCGGGCGTTATCTGGATGCTGCTTTTTCTGGTAACACCCATCCTGATGATGATCTACGTGTCCTTCTGGACGC
AGACGACCTTCAAGATCACGCCTGATCTTACCATCAAGAGCTGGATCACCTTCTTCACCACGGACACCTATCTCAGCGCT
CTTTGGACGACGGTCAGGATCTGGTTGATCGTGCTTGTCTCGACGCTGGTCGTCGGCTATCCGGCGGCCCTCTATGTCGG
CCTCTTCGTCAAGAACAAGACAATCCAGACGGCGCTTCTCGTCCTCTGCGTCATCCCTTTCTGGACCTCTTTCCTGATCC
GCGTGCTCGCCTGGCGGCCGATGTTGGGCAAGGAGGGGGCGATCAATCTCATCCTGCAGGGTCTCGGCATCATCAACCAG
CCGATCGAGGTGCTGCTGTTCTCCGAACTCTCGGTCATCATCGGCATGACGCAGATCTATTGCGTCTTCATGGTCGGGCC
GATCGCCTTCATGATGGGCCGCATCGACCAGAGCGTCATCGAAGCGGCGCAGGATCTCGGTGCGGGCTTCTGGCGCATCT
TCCGCACCATCATCCTGCCGCTCACCATGCCGGGCGTGGTGGTCGGCGCGATCTTCGTCTCCGTCATGGTGCTTGGTGAG
TTCGCGACGGCCGCGGCCCTTTCCGGCCGCAAGGTGAACCTCCTCGGCAATATCATCGTCACCCAGGTCGGATCGCTGAA
ATGGGCCTTCGCCGCCGTCGTCGGCGTCGTGCTCACCGTGCTCATGGGCATTGTGGTCGCAGCACTCCTGCGCGTCGTCG
ATCTCAGGAAGGAGCTCTGA

Upstream 100 bases:

>100_bases
GTGCGCCCCAGTCGCGCTACGCACTCGTCTGGAAGCGCCGGGATGCCCTCGTTTACGCCTGACACTCTTACCGCGCAGCC
GCGGGACGGGAGACTGACTG

Downstream 100 bases:

>100_bases
CATGCAGGCAGGCGGCATCAAGCTTTCACTCGGCGTCTACACCGCGCTCTTCCTGATCTTCCTTTACGCACCGATGGTGG
TGCTCGCCATTCTGTCTTTC

Product: putative permease component of ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSISATTAETETTVEPVRKASGKTLWLLAPGVIWMLLFLVTPILMMIYVSFWTQTTFKITPDLTIKSWITFFTTDTYLSA
LWTTVRIWLIVLVSTLVVGYPAALYVGLFVKNKTIQTALLVLCVIPFWTSFLIRVLAWRPMLGKEGAINLILQGLGIINQ
PIEVLLFSELSVIIGMTQIYCVFMVGPIAFMMGRIDQSVIEAAQDLGAGFWRIFRTIILPLTMPGVVVGAIFVSVMVLGE
FATAAALSGRKVNLLGNIIVTQVGSLKWAFAAVVGVVLTVLMGIVVAALLRVVDLRKEL

Sequences:

>Translated_299_residues
MSISATTAETETTVEPVRKASGKTLWLLAPGVIWMLLFLVTPILMMIYVSFWTQTTFKITPDLTIKSWITFFTTDTYLSA
LWTTVRIWLIVLVSTLVVGYPAALYVGLFVKNKTIQTALLVLCVIPFWTSFLIRVLAWRPMLGKEGAINLILQGLGIINQ
PIEVLLFSELSVIIGMTQIYCVFMVGPIAFMMGRIDQSVIEAAQDLGAGFWRIFRTIILPLTMPGVVVGAIFVSVMVLGE
FATAAALSGRKVNLLGNIIVTQVGSLKWAFAAVVGVVLTVLMGIVVAALLRVVDLRKEL
>Mature_298_residues
SISATTAETETTVEPVRKASGKTLWLLAPGVIWMLLFLVTPILMMIYVSFWTQTTFKITPDLTIKSWITFFTTDTYLSAL
WTTVRIWLIVLVSTLVVGYPAALYVGLFVKNKTIQTALLVLCVIPFWTSFLIRVLAWRPMLGKEGAINLILQGLGIINQP
IEVLLFSELSVIIGMTQIYCVFMVGPIAFMMGRIDQSVIEAAQDLGAGFWRIFRTIILPLTMPGVVVGAIFVSVMVLGEF
ATAAALSGRKVNLLGNIIVTQVGSLKWAFAAVVGVVLTVLMGIVVAALLRVVDLRKEL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=254, Percent_Identity=33.4645669291339, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787080, Length=232, Percent_Identity=33.1896551724138, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787713, Length=265, Percent_Identity=25.6603773584906, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32832; Mature: 32701

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISATTAETETTVEPVRKASGKTLWLLAPGVIWMLLFLVTPILMMIYVSFWTQTTFKIT
CCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
PDLTIKSWITFFTTDTYLSALWTTVRIWLIVLVSTLVVGYPAALYVGLFVKNKTIQTALL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VLCVIPFWTSFLIRVLAWRPMLGKEGAINLILQGLGIINQPIEVLLFSELSVIIGMTQIY
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
CVFMVGPIAFMMGRIDQSVIEAAQDLGAGFWRIFRTIILPLTMPGVVVGAIFVSVMVLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FATAAALSGRKVNLLGNIIVTQVGSLKWAFAAVVGVVLTVLMGIVVAALLRVVDLRKEL
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SISATTAETETTVEPVRKASGKTLWLLAPGVIWMLLFLVTPILMMIYVSFWTQTTFKIT
CCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
PDLTIKSWITFFTTDTYLSALWTTVRIWLIVLVSTLVVGYPAALYVGLFVKNKTIQTALL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VLCVIPFWTSFLIRVLAWRPMLGKEGAINLILQGLGIINQPIEVLLFSELSVIIGMTQIY
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
CVFMVGPIAFMMGRIDQSVIEAAQDLGAGFWRIFRTIILPLTMPGVVVGAIFVSVMVLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FATAAALSGRKVNLLGNIIVTQVGSLKWAFAAVVGVVLTVLMGIVVAALLRVVDLRKEL
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]