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The map label for this gene is pepP [C]

Identifier: 116248730

GI number: 116248730

Start: 53221

End: 54402

Strand: Direct

Name: pepP [C]

Synonym: pRL120054

Alternate gene names: 116248730

Gene position: 53221-54402 (Clockwise)

Preceding gene: 116248729

Following gene: 116248731

Centisome position: 6.12

GC content: 62.35

Gene sequence:

>1182_bases
ATGACCCAGCCCAAACTCAAATTCTCGCTCGGCGAATATGCCGCGCGGCTGGAAAAGACACGGCGTGCCATGGAGGCGAA
GGGTGTCGACCTGCTGATCGTCAGCGACCCGTCGAACATGGCCTGGTTGACCGGTTATGACGGCTGGTCCTTCTACGTGC
ACCAGGCGGTGATCGTGCCGCCGCAGGGCGAGCCGATCTGGTTCGGCCGCGGCCAGGATGCCAATGGCGCCAAGTTCACC
GCCTATCTGAAACACGACAACATCGTCGGTTATCCCGATCACTATGTGCAGTCGACCGAGCGCCACCCGATGGACTACCT
TTCGGGCATCCTGACCGAGCGCGGCTTCGGCAAGCTGACGATCGGCGTCGAGATGGACAATTACTGGTTTTCGGCGGCGG
CCTTTGCGGCGCTGCAGAAGCACTTGCCGAATGCGCGTTTCGTCGACGCGACCGCCCTCGTCAACTGGCAGCGAGCCGTC
AAAAGCGAGACCGAGATCGGCTACATGCGCAATGCCGCCCGTATCGTCGAGGCGATGCACGCCCGCATCTTCGACAAGAT
CGAGGTCGGCATGCGCAAGTGCGATCTGGTCGCGGAAATCTATGATGCCGGCACCCGCGGCGTCGACGGCATTGGCGGTG
ATTATCCGGCGATCGTGCCGCTGCTGCCGTCCGGCGTCGAAGCATCCGCACCGCACCTGACCTGGGACGACCGACCGCTG
AAGAAAGGCGAGGGCACCTTCTTCGAGATCGCCGGCTGCTACAACCGCTATCATCTGCCGCTGTCGCGCACCGTCTTCCT
CGGCAAGCCGACGCAGGCCTTCCTCGATGCCGAAAAAGCGACGCTGGAGGGCATGGAAGCCGGTCTTGCGGTCGCCAGAC
CTGGCAATAGCTGTGAGGATATCGCCAACGCCTTCTTCGCGGTGCTGAAGAAATACGGCATCGTCAAGGACAACCGCACG
GGTTACCCGATCGGCCTTTCCTATCCGCCGGACTGGGGCGAGCGCACCATGAGCCTGCGACCGGGCGATCGGACGGAGCT
GAAGCCCGGTATGACCTTCCATTTCATGACCGGCCTCTGGCTCGAAGACATGGGCTTCGAGACGACCGAGAGCATCCTCA
TCACCGACAGCGGTGTCGAGTGCTTCGCGAACGTGCCGCGCAGACTGATGGTCAAGGATTGA

Upstream 100 bases:

>100_bases
CCGCCATCGCCACGCTCGCCTTTGCCCGCGCCGGCGCGGTGAAGGCCGGGACCGCATTCGAAAGCTGACCGGCGCGGCGC
CGGAAGAGAAGGGAAGAAAC

Downstream 100 bases:

>100_bases
GGCTGTCATGACAGAGATTGGCTTGCGGCCGTCGCCGATCAGCGCGACGGTCAATTTCGCCGCCGAGGGCGTCCAGCACG
GATTCCTGCGCCTGCCTTAC

Product: hypothetical protein

Products: N-terminal amino acid including proline [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTQPKLKFSLGEYAARLEKTRRAMEAKGVDLLIVSDPSNMAWLTGYDGWSFYVHQAVIVPPQGEPIWFGRGQDANGAKFT
AYLKHDNIVGYPDHYVQSTERHPMDYLSGILTERGFGKLTIGVEMDNYWFSAAAFAALQKHLPNARFVDATALVNWQRAV
KSETEIGYMRNAARIVEAMHARIFDKIEVGMRKCDLVAEIYDAGTRGVDGIGGDYPAIVPLLPSGVEASAPHLTWDDRPL
KKGEGTFFEIAGCYNRYHLPLSRTVFLGKPTQAFLDAEKATLEGMEAGLAVARPGNSCEDIANAFFAVLKKYGIVKDNRT
GYPIGLSYPPDWGERTMSLRPGDRTELKPGMTFHFMTGLWLEDMGFETTESILITDSGVECFANVPRRLMVKD

Sequences:

>Translated_393_residues
MTQPKLKFSLGEYAARLEKTRRAMEAKGVDLLIVSDPSNMAWLTGYDGWSFYVHQAVIVPPQGEPIWFGRGQDANGAKFT
AYLKHDNIVGYPDHYVQSTERHPMDYLSGILTERGFGKLTIGVEMDNYWFSAAAFAALQKHLPNARFVDATALVNWQRAV
KSETEIGYMRNAARIVEAMHARIFDKIEVGMRKCDLVAEIYDAGTRGVDGIGGDYPAIVPLLPSGVEASAPHLTWDDRPL
KKGEGTFFEIAGCYNRYHLPLSRTVFLGKPTQAFLDAEKATLEGMEAGLAVARPGNSCEDIANAFFAVLKKYGIVKDNRT
GYPIGLSYPPDWGERTMSLRPGDRTELKPGMTFHFMTGLWLEDMGFETTESILITDSGVECFANVPRRLMVKD
>Mature_392_residues
TQPKLKFSLGEYAARLEKTRRAMEAKGVDLLIVSDPSNMAWLTGYDGWSFYVHQAVIVPPQGEPIWFGRGQDANGAKFTA
YLKHDNIVGYPDHYVQSTERHPMDYLSGILTERGFGKLTIGVEMDNYWFSAAAFAALQKHLPNARFVDATALVNWQRAVK
SETEIGYMRNAARIVEAMHARIFDKIEVGMRKCDLVAEIYDAGTRGVDGIGGDYPAIVPLLPSGVEASAPHLTWDDRPLK
KGEGTFFEIAGCYNRYHLPLSRTVFLGKPTQAFLDAEKATLEGMEAGLAVARPGNSCEDIANAFFAVLKKYGIVKDNRTG
YPIGLSYPPDWGERTMSLRPGDRTELKPGMTFHFMTGLWLEDMGFETTESILITDSGVECFANVPRRLMVKD

Specific function: Unknown

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24 family [H]

Homologues:

Organism=Escherichia coli, GI1789275, Length=345, Percent_Identity=25.2173913043478, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788728, Length=258, Percent_Identity=24.4186046511628, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR000994 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: 3.4.11.9 [C]

Molecular weight: Translated: 43756; Mature: 43625

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01159 WW_DOMAIN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPKLKFSLGEYAARLEKTRRAMEAKGVDLLIVSDPSNMAWLTGYDGWSFYVHQAVIVP
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCEEEEEEEEEEEC
PQGEPIWFGRGQDANGAKFTAYLKHDNIVGYPDHYVQSTERHPMDYLSGILTERGFGKLT
CCCCEEEEECCCCCCCCEEEEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCEEE
IGVEMDNYWFSAAAFAALQKHLPNARFVDATALVNWQRAVKSETEIGYMRNAARIVEAMH
EEEEECCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ARIFDKIEVGMRKCDLVAEIYDAGTRGVDGIGGDYPAIVPLLPSGVEASAPHLTWDDRPL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KKGEGTFFEIAGCYNRYHLPLSRTVFLGKPTQAFLDAEKATLEGMEAGLAVARPGNSCED
CCCCCCEEEEEECCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCCHHH
IANAFFAVLKKYGIVKDNRTGYPIGLSYPPDWGERTMSLRPGDRTELKPGMTFHFMTGLW
HHHHHHHHHHHHCCEECCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCEEEEHHCHH
LEDMGFETTESILITDSGVECFANVPRRLMVKD
HHHCCCCCCCEEEEECCCHHHHHCCCCEEECCC
>Mature Secondary Structure 
TQPKLKFSLGEYAARLEKTRRAMEAKGVDLLIVSDPSNMAWLTGYDGWSFYVHQAVIVP
CCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCEEEEEEEEEEEC
PQGEPIWFGRGQDANGAKFTAYLKHDNIVGYPDHYVQSTERHPMDYLSGILTERGFGKLT
CCCCEEEEECCCCCCCCEEEEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCEEE
IGVEMDNYWFSAAAFAALQKHLPNARFVDATALVNWQRAVKSETEIGYMRNAARIVEAMH
EEEEECCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ARIFDKIEVGMRKCDLVAEIYDAGTRGVDGIGGDYPAIVPLLPSGVEASAPHLTWDDRPL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KKGEGTFFEIAGCYNRYHLPLSRTVFLGKPTQAFLDAEKATLEGMEAGLAVARPGNSCED
CCCCCCEEEEEECCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCCHHH
IANAFFAVLKKYGIVKDNRTGYPIGLSYPPDWGERTMSLRPGDRTELKPGMTFHFMTGLW
HHHHHHHHHHHHCCEECCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCEEEEHHCHH
LEDMGFETTESILITDSGVECFANVPRRLMVKD
HHHCCCCCCCEEEEECCCHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+ [C]

Kcat value (1/min): 8100 [C]

Specific activity: 3.45

Km value (mM): 0.22 {(4-nitro)Phe-Pro-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} 3 {(4-nitro)Phe-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} [C]

Substrates: N-terminal amino acid including proline [C]

Specific reaction: Release of any N-terminal amino acid including proline that mislinked with proline even from a dipeptide or tripeptide [C]

General reaction: carboxylic acid amide hydrolysis [C]

Inhibitor: thiazolidide acid; -2-hydroxy -3-amino acidpyrrolidide acid; Pro-methyl ester acid; Pro-Phe-methyl ester acid; 2-hydroxy -3-aminoacyl-Pro-OH dipeptides [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]