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The map label for this gene is artJ [C]

Identifier: 116248724

GI number: 116248724

Start: 48059

End: 48907

Strand: Direct

Name: artJ [C]

Synonym: pRL120048

Alternate gene names: 116248724

Gene position: 48059-48907 (Clockwise)

Preceding gene: 116248723

Following gene: 116248725

Centisome position: 5.52

GC content: 63.84

Gene sequence:

>849_bases
ATGAAAACAGGATATTTTTTGTGCGCCGCCAGCCTGTCGGTGCTTCTGGTCACGGCGGTCGCCCCGGCGTCCGCCGCCGA
TGACAAGCTCGAGCAGCTGAAGGAGCAAGGCTTTGCGCGTATCGCCATCGCCAACGAGCCGCCGTTCACCGCTGTCGGCG
CCGACGGCAAGGTGTCCGGCGCTGCCCCCGATGTGGCGCGCGCAATCTTCGAAAAGCTGGGCGTCAAGGAAGTGGTCGCC
TCGATCTCGGAATATGGCGCAATGATCCCCGGCCTGCAGGCCGGCCGCCACGACGCGATCACCGCAGGCCTCTTCATGAA
GCCTGAGCGCTGCAACGCCGTCGCCTATTCCGAACCGATCCTTTGCGACGCTGAAGCTTTCGCGCTCAAGAAGGGCAACC
CACTGAAGCTGACGAGCTACAAGGACATCGCCGACAATCCGGACGCCAAGATCGGCGCGCCGGGCGGCGGCACCGAGGAG
AAGCTGGCGCTCGAGGCCGGCGTGCCGCGCGACCGCGTCATCGTCGTTCCTGATGGCCAGAGCGGCATCAAGATGCTGCA
GGACGGCCGCATCGACGTCTACTCGCTGCCGGTTCTGTCGATCCACGATCTGATGGCCAAGGCGAACGATCCGAACCTTG
AAACCGTCGCACCCGTCGTCAATGCGCCGGTCTATTGCGATGGTGCGGCTTTCCGCAAGCAGGACGTTGCGCTCCGCGAC
GCCTTCGACGTCGAGCTGAAGAAGCTGAAGGAATCCGGCGAATTCGCCAAGATCATCGAGCCCTACGGCTTCTCGGCGAA
GGCGGCGATGTCGACGAGCCGCGAAAAGCTCTGCGCTGCGGCGAAGTAA

Upstream 100 bases:

>100_bases
AGACCTTCCTGCGCAAGATCATTGCGGCGGGGCACCGCATCTGACCTCAGACATTGCCCGCAAGAACAAAACTCAAGGAA
AATCAGAGGAGTTGGGAACG

Downstream 100 bases:

>100_bases
CAAAGGGTAAGCAACCGGCCATGCCCCAGGCGTGGCCGGATCGCCCTCGAACGATCTCCCAAAGCGCGTCGCGATCTTTC
AGATGCACTCCTCGCGCTTT

Product: putative substrate-binding component of ABC transporter

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKTGYFLCAASLSVLLVTAVAPASAADDKLEQLKEQGFARIAIANEPPFTAVGADGKVSGAAPDVARAIFEKLGVKEVVA
SISEYGAMIPGLQAGRHDAITAGLFMKPERCNAVAYSEPILCDAEAFALKKGNPLKLTSYKDIADNPDAKIGAPGGGTEE
KLALEAGVPRDRVIVVPDGQSGIKMLQDGRIDVYSLPVLSIHDLMAKANDPNLETVAPVVNAPVYCDGAAFRKQDVALRD
AFDVELKKLKESGEFAKIIEPYGFSAKAAMSTSREKLCAAAK

Sequences:

>Translated_282_residues
MKTGYFLCAASLSVLLVTAVAPASAADDKLEQLKEQGFARIAIANEPPFTAVGADGKVSGAAPDVARAIFEKLGVKEVVA
SISEYGAMIPGLQAGRHDAITAGLFMKPERCNAVAYSEPILCDAEAFALKKGNPLKLTSYKDIADNPDAKIGAPGGGTEE
KLALEAGVPRDRVIVVPDGQSGIKMLQDGRIDVYSLPVLSIHDLMAKANDPNLETVAPVVNAPVYCDGAAFRKQDVALRD
AFDVELKKLKESGEFAKIIEPYGFSAKAAMSTSREKLCAAAK
>Mature_282_residues
MKTGYFLCAASLSVLLVTAVAPASAADDKLEQLKEQGFARIAIANEPPFTAVGADGKVSGAAPDVARAIFEKLGVKEVVA
SISEYGAMIPGLQAGRHDAITAGLFMKPERCNAVAYSEPILCDAEAFALKKGNPLKLTSYKDIADNPDAKIGAPGGGTEE
KLALEAGVPRDRVIVVPDGQSGIKMLQDGRIDVYSLPVLSIHDLMAKANDPNLETVAPVVNAPVYCDGAAFRKQDVALRD
AFDVELKKLKESGEFAKIIEPYGFSAKAAMSTSREKLCAAAK

Specific function: Probably part of the binding-protein-dependent transport system y4tEFGH for an amino acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=222, Percent_Identity=24.7747747747748, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787088, Length=225, Percent_Identity=24.8888888888889, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788228, Length=232, Percent_Identity=28.448275862069, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014337
- InterPro:   IPR001638 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29682; Mature: 29682

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTGYFLCAASLSVLLVTAVAPASAADDKLEQLKEQGFARIAIANEPPFTAVGADGKVSG
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCEEECCCCCCCC
AAPDVARAIFEKLGVKEVVASISEYGAMIPGLQAGRHDAITAGLFMKPERCNAVAYSEPI
CCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECEEECCHHCCEEECCCCE
LCDAEAFALKKGNPLKLTSYKDIADNPDAKIGAPGGGTEEKLALEAGVPRDRVIVVPDGQ
EECCHHHEECCCCCEEEECCCCCCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEEECCC
SGIKMLQDGRIDVYSLPVLSIHDLMAKANDPNLETVAPVVNAPVYCDGAAFRKQDVALRD
HHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHCCCEEECCCHHHHHCHHHHH
AFDVELKKLKESGEFAKIIEPYGFSAKAAMSTSREKLCAAAK
HHHHHHHHHHHCCCEEEHHCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTGYFLCAASLSVLLVTAVAPASAADDKLEQLKEQGFARIAIANEPPFTAVGADGKVSG
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCEEECCCCCCCC
AAPDVARAIFEKLGVKEVVASISEYGAMIPGLQAGRHDAITAGLFMKPERCNAVAYSEPI
CCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECEEECCHHCCEEECCCCE
LCDAEAFALKKGNPLKLTSYKDIADNPDAKIGAPGGGTEEKLALEAGVPRDRVIVVPDGQ
EECCHHHEECCCCCEEEECCCCCCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEEECCC
SGIKMLQDGRIDVYSLPVLSIHDLMAKANDPNLETVAPVVNAPVYCDGAAFRKQDVALRD
HHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHCCCEEECCCHHHHHCHHHHH
AFDVELKKLKESGEFAKIIEPYGFSAKAAMSTSREKLCAAAK
HHHHHHHHHHHCCCEEEHHCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]