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The map label for this gene is gcvA [H]

Identifier: 115353108

GI number: 115353108

Start: 3375482

End: 3376366

Strand: Direct

Name: gcvA [H]

Synonym: Bamb_3057

Alternate gene names: 115353108

Gene position: 3375482-3376366 (Clockwise)

Preceding gene: 115353105

Following gene: 162389965

Centisome position: 94.91

GC content: 70.28

Gene sequence:

>885_bases
ATGGCACGCGATCTCCCGCCCTTTTCCGCGCTTCGGGCCTTTGAAGCAGCGGCCCGACACGAAAGCTTCAGCGCCGCCGG
CGACGAGCTGCATGTGACTCACGGTGCCATCAGCCGGCAGATCGCCGGCTTCGAGGCGTGGCTCGGCAAGCCGGTGTTCC
ATCGCTACGGCAAGCGCGTGAGGCTCACCGACGAAGGCCGCCGTTATCTGGACACCGTGCGCGCCGCGTTCGACAGCATC
GCGCTCGCCACCGAGCAGTTGCGCAACACGGGTGCCGCGCGCGTGCTGCGCATCAATGCGCTGCCCACCTTCGCGATGAA
ATGGCTGCTGCCGCGCCTGTCGCGGTTCCAGCGCGACGTGCCGAACGTCGAACTGAAGCTGTCCACGTCGAACGCGCCGC
TCGACGCGCTCGACGGCTTCGACGTCGCAATCCGCCGCGGCCCCGGCCACTGGCCGAACTGCACGAGCGGCCACTTCCTC
GACGAAAGCGTGATTCCGGTCTGCAGCCCCGCGCTGCTCAAGCGCGCGCCGATCACCCGCGCGGACGATCTCGCCCGGCA
CGTGCTGCTGCATTCGGATACGCGGCCGGAAGGCTGGCGCGACTGGTTCGCGGCGGCCGGCGTGACGCCGAAGGGGCGCA
AGCGGCAGTCGTTCGACCACTTCTACCTGGCGCTGCAGGCGGCCGTCGACGGGCTGGGTGTCGCGCTCGGGCCGCTGCCG
CTGATCGACGACGAACTCGCGAGCGGGCGCCTGGTGATGCCGCTCGACGGGCCGCGCATCGCGACGCGCAGCTATTGGTG
GATCGCGCCGCGCGCGCCGGCGGACGATCCGCTCGTCGCGCAGTTCTGTGCGTGGCTGCAGGCGCAGTCGAACACCGGCG
CGTAA

Upstream 100 bases:

>100_bases
TGCATGGGGCTCCTCATCAAACGTGCCCCGAGATTACCGCCGTTCGGAATGCCGGCAAAACGATTTTATCGACGGGGCCT
TGTGATCTAGAATCACAAGC

Downstream 100 bases:

>100_bases
GGACGCACCGGCGGGCGGCATTGCACCGCCCGCCCCCCAATCAAACCACCGGCCCCGGTTCCTTTTCCTTGCGCAGGATC
GCGTACAGGATGATCGCGCC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRVRLTDEGRRYLDTVRAAFDSI
ALATEQLRNTGAARVLRINALPTFAMKWLLPRLSRFQRDVPNVELKLSTSNAPLDALDGFDVAIRRGPGHWPNCTSGHFL
DESVIPVCSPALLKRAPITRADDLARHVLLHSDTRPEGWRDWFAAAGVTPKGRKRQSFDHFYLALQAAVDGLGVALGPLP
LIDDELASGRLVMPLDGPRIATRSYWWIAPRAPADDPLVAQFCAWLQAQSNTGA

Sequences:

>Translated_294_residues
MARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRVRLTDEGRRYLDTVRAAFDSI
ALATEQLRNTGAARVLRINALPTFAMKWLLPRLSRFQRDVPNVELKLSTSNAPLDALDGFDVAIRRGPGHWPNCTSGHFL
DESVIPVCSPALLKRAPITRADDLARHVLLHSDTRPEGWRDWFAAAGVTPKGRKRQSFDHFYLALQAAVDGLGVALGPLP
LIDDELASGRLVMPLDGPRIATRSYWWIAPRAPADDPLVAQFCAWLQAQSNTGA
>Mature_293_residues
ARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRVRLTDEGRRYLDTVRAAFDSIA
LATEQLRNTGAARVLRINALPTFAMKWLLPRLSRFQRDVPNVELKLSTSNAPLDALDGFDVAIRRGPGHWPNCTSGHFLD
ESVIPVCSPALLKRAPITRADDLARHVLLHSDTRPEGWRDWFAAAGVTPKGRKRQSFDHFYLALQAAVDGLGVALGPLPL
IDDELASGRLVMPLDGPRIATRSYWWIAPRAPADDPLVAQFCAWLQAQSNTGA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=35.4948805460751, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=33.6769759450172, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=29.8305084745763, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=29.7945205479452, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787128, Length=144, Percent_Identity=34.0277777777778, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1786401, Length=250, Percent_Identity=28.4, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI157672245, Length=210, Percent_Identity=29.5238095238095, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081978, Length=260, Percent_Identity=26.5384615384615, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=270, Percent_Identity=26.2962962962963, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790011, Length=283, Percent_Identity=22.9681978798587, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32441; Mature: 32310

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRV
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHCCCEE
RLTDEGRRYLDTVRAAFDSIALATEQLRNTGAARVLRINALPTFAMKWLLPRLSRFQRDV
EECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
PNVELKLSTSNAPLDALDGFDVAIRRGPGHWPNCTSGHFLDESVIPVCSPALLKRAPITR
CCEEEEEECCCCCCHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
ADDLARHVLLHSDTRPEGWRDWFAAAGVTPKGRKRQSFDHFYLALQAAVDGLGVALGPLP
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCC
LIDDELASGRLVMPLDGPRIATRSYWWIAPRAPADDPLVAQFCAWLQAQSNTGA
CCCHHHCCCEEEEECCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRV
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHCCCEE
RLTDEGRRYLDTVRAAFDSIALATEQLRNTGAARVLRINALPTFAMKWLLPRLSRFQRDV
EECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
PNVELKLSTSNAPLDALDGFDVAIRRGPGHWPNCTSGHFLDESVIPVCSPALLKRAPITR
CCEEEEEECCCCCCHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
ADDLARHVLLHSDTRPEGWRDWFAAAGVTPKGRKRQSFDHFYLALQAAVDGLGVALGPLP
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCC
LIDDELASGRLVMPLDGPRIATRSYWWIAPRAPADDPLVAQFCAWLQAQSNTGA
CCCHHHCCCEEEEECCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]