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The map label for this gene is ttcA [H]

Identifier: 115353078

GI number: 115353078

Start: 3345665

End: 3346669

Strand: Direct

Name: ttcA [H]

Synonym: Bamb_3027

Alternate gene names: 115353078

Gene position: 3345665-3346669 (Clockwise)

Preceding gene: 115353077

Following gene: 115353079

Centisome position: 94.07

GC content: 66.57

Gene sequence:

>1005_bases
ATGAACGCCCCCCACACGCATGACACGGTGGCCGATGGCGCCGCCATCGAAGCCACCGTCGCCGACATCGCCGACACCGG
CCGCCGCGCGCTGACGCGCCGCGAGCAGAAGGAAGCGTACGAGAACAACAAGCTGTTCAAGCGGATCGTGCGCCAGGTCG
GCCAGGCGATCGGCGACTACAACATGATCGAGCAGGGCGACAAGGTGATGGTGTGCCTGTCGGGCGGCAAGGACAGCTAC
GCGATGCTCGACGTGTTGCTGCGCCTGCGCGAGCGCGCGCCGATCGACTTCGACATCGTCGCGGTCAACCTCGACCAGAA
GCAGCCGGGTTTCCCCGAGCACGTGCTGCCCGAATACCTGACGCAGATCGGCGTGCCGTTCCACATCGAGAACCAGGACA
CCTACAGCATCGTCAAGCGGCTCGTGCCCGAGGGCAAGACGACCTGCTCGCTGTGCTCGCGGCTGCGCCGCGGGATCCTG
TATCGCGTGGCCGGCGAGCTCGGCGCGACCAAGATCGCGCTCGGCCACCACCGGGACGACATCCTGCAAACCCTGCTGCT
CAACATGTTCTACGGCGGCAAGCTGAAGGGGATGCCGCCGAAGCTGCAGTCGGACGACGGCAGGAACGTCGTGATCCGCC
CGCTCGCGTACGTGAAGGAGACCGATCTCGAGAAATTCGCCGAGCTGCGCGAATTCCCGATCATCCCGTGCAACCTGTGC
GGCAGCCAGCCGAACCTGAAGCGCGCGGAAATGAAGGCACTGATCCGCGACTGGGACAAGCGCTTCCCGGGCCGCGTCGA
CAACATGTTCAGCGCGCTCGCGAACGTCGTGCCGTCGCACCTGATGGACACGACCCAGTTCCCGTTCGCGTCGCTGCGCG
CGACCGGCCAGGCCGACCCGCAGGGCGACATCGCGTTCGACGAGGAACCGTGCGCGTCCGGCGACGACACCGCCACGCGC
GACGGCGCCCGACCGATCTCGATCGTCCAGTTCGACGACCTGTAA

Upstream 100 bases:

>100_bases
TCGTGCGCGCGGTACGTGTCCGTACCGAGAAACCGGACGCCTATCCGGACTGCGACGCCGTCGGCGTCGAAGTCTTTCGC
ATCAAGGACGAGGAGCGCGC

Downstream 100 bases:

>100_bases
GCAGGGCCCGAACACCCGCCGGAACCGCGGCCCGCGGCCCTGTTCCGGCGCTGTCGCCCGGCCGCGACAGGCCCGGAACG
CCAGGGGCCGCATGCTAAAA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MNAPHTHDTVADGAAIEATVADIADTGRRALTRREQKEAYENNKLFKRIVRQVGQAIGDYNMIEQGDKVMVCLSGGKDSY
AMLDVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLTQIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGIL
YRVAGELGATKIALGHHRDDILQTLLLNMFYGGKLKGMPPKLQSDDGRNVVIRPLAYVKETDLEKFAELREFPIIPCNLC
GSQPNLKRAEMKALIRDWDKRFPGRVDNMFSALANVVPSHLMDTTQFPFASLRATGQADPQGDIAFDEEPCASGDDTATR
DGARPISIVQFDDL

Sequences:

>Translated_334_residues
MNAPHTHDTVADGAAIEATVADIADTGRRALTRREQKEAYENNKLFKRIVRQVGQAIGDYNMIEQGDKVMVCLSGGKDSY
AMLDVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLTQIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGIL
YRVAGELGATKIALGHHRDDILQTLLLNMFYGGKLKGMPPKLQSDDGRNVVIRPLAYVKETDLEKFAELREFPIIPCNLC
GSQPNLKRAEMKALIRDWDKRFPGRVDNMFSALANVVPSHLMDTTQFPFASLRATGQADPQGDIAFDEEPCASGDDTATR
DGARPISIVQFDDL
>Mature_334_residues
MNAPHTHDTVADGAAIEATVADIADTGRRALTRREQKEAYENNKLFKRIVRQVGQAIGDYNMIEQGDKVMVCLSGGKDSY
AMLDVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLTQIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGIL
YRVAGELGATKIALGHHRDDILQTLLLNMFYGGKLKGMPPKLQSDDGRNVVIRPLAYVKETDLEKFAELREFPIIPCNLC
GSQPNLKRAEMKALIRDWDKRFPGRVDNMFSALANVVPSHLMDTTQFPFASLRATGQADPQGDIAFDEEPCASGDDTATR
DGARPISIVQFDDL

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=274, Percent_Identity=67.5182481751825, Blast_Score=407, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI193211090, Length=278, Percent_Identity=30.2158273381295, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=237, Percent_Identity=28.6919831223629, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24651972, Length=248, Percent_Identity=28.2258064516129, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 37225; Mature: 37225

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPHTHDTVADGAAIEATVADIADTGRRALTRREQKEAYENNKLFKRIVRQVGQAIGDY
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NMIEQGDKVMVCLSGGKDSYAMLDVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYL
HHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHH
TQIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRDD
HHCCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHH
ILQTLLLNMFYGGKLKGMPPKLQSDDGRNVVIRPLAYVKETDLEKFAELREFPIIPCNLC
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCHHCC
GSQPNLKRAEMKALIRDWDKRFPGRVDNMFSALANVVPSHLMDTTQFPFASLRATGQADP
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCC
QGDIAFDEEPCASGDDTATRDGARPISIVQFDDL
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MNAPHTHDTVADGAAIEATVADIADTGRRALTRREQKEAYENNKLFKRIVRQVGQAIGDY
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NMIEQGDKVMVCLSGGKDSYAMLDVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYL
HHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHH
TQIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRDD
HHCCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHH
ILQTLLLNMFYGGKLKGMPPKLQSDDGRNVVIRPLAYVKETDLEKFAELREFPIIPCNLC
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCHHCC
GSQPNLKRAEMKALIRDWDKRFPGRVDNMFSALANVVPSHLMDTTQFPFASLRATGQADP
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCC
QGDIAFDEEPCASGDDTATRDGARPISIVQFDDL
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA