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The map label for this gene is gcvA [H]

Identifier: 115353049

GI number: 115353049

Start: 3316361

End: 3317281

Strand: Direct

Name: gcvA [H]

Synonym: Bamb_2998

Alternate gene names: 115353049

Gene position: 3316361-3317281 (Clockwise)

Preceding gene: 115353046

Following gene: 115353053

Centisome position: 93.25

GC content: 73.83

Gene sequence:

>921_bases
ATGCGACGCATCCACCTTCCCCCGCTGCAGACGCTGCGCGCGTTCGAAGCCGCCGTGCGGCTGCAGAGCTTCACGCGCGC
GGCCGACGAACTCGCGCTCACGCAGGGCGCGGTCAGCCAGCACATCCGCGCGCTCGAAGCGCAGCTCGGTCATCCGCTGT
TCACGCGCGAGCGCAACGGCGCCACGCCGACGCATGCCGCGCACGCGCTCGTGCTGCAGGTGCGCCAGGGCCTGAGCGTG
CTCGAACGCGCGTTCGAGCCGGCGCTCGCCGTGCGCGACCGCCCGCGCACCTGCGACGTCACGCTGAACGTGAGCGTGCT
GCCGAGCGTCGCCGAGCGCTGGCTCGCGCCGCGCCTGCCGCGCTTCGCGGCCGCGCATCCGCACATCGCGATCGCGCTGC
ATCCGGACGCGGCGCTCGCGCCGCTGCGCAAGCGCGACCGCATCGACGTCGCGCTGCGCTACGGGCCTGGCACCTGGCCG
GGCGTCGTCGCCGAGAAGCTGATGAACGAGACGGTCTTTCCGGTCGCGAGCCCCGCGTACCGCAATCGCGACGGCATCGC
GCCGCGCACGCCAGCCGATCTCGTGCGCGCGACCCTGCTGCGCCACCCCGCGCAGCCGTGGGAACCGTGGTTCCAGGCCG
CTCGCCTCGACCTCGTCGAATCCGCGCGCGCGCCGCGCTTCACCGATGCGAACGCGCTGATCGACGCGGCGCTGAAAGGA
CGCGGCGTCGCGCTCGCGCGCCGCTCGCTGATCGAACCCGAACTCGCGGCCGGCACGCTCGTGCGCGTGTCGACGGTGCG
CGTGACCGACGTGTATGCGCACTACGTGGTGTGGCGCCCCGGCCATCCGCACGAAGCGGCGATCCGCACGTGGCTCGACT
GGCTGCGCAGCGAAGTGCGGCGCAGGACGCCGCGACGCTGA

Upstream 100 bases:

>100_bases
CGGTGGACTCGTTGAAAGACGGAACCGAGTGTAGGAGCGCGCGGCCGGCCGCCACAAACGATGAATTGTCGTGCTCGGGC
ATTAGTGCTACTAATGCGTG

Downstream 100 bases:

>100_bases
CGCGCTCAGGCCCGCGCCGGCATCGCCACCTTGTCGAGCGTGATCGGCAGATCGCGCACGCGCACGCCCGTCGCGTGATA
GACGGCGTTGGCAATCGCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGHPLFTRERNGATPTHAAHALVLQVRQGLSV
LERAFEPALAVRDRPRTCDVTLNVSVLPSVAERWLAPRLPRFAAAHPHIAIALHPDAALAPLRKRDRIDVALRYGPGTWP
GVVAEKLMNETVFPVASPAYRNRDGIAPRTPADLVRATLLRHPAQPWEPWFQAARLDLVESARAPRFTDANALIDAALKG
RGVALARRSLIEPELAAGTLVRVSTVRVTDVYAHYVVWRPGHPHEAAIRTWLDWLRSEVRRRTPRR

Sequences:

>Translated_306_residues
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGHPLFTRERNGATPTHAAHALVLQVRQGLSV
LERAFEPALAVRDRPRTCDVTLNVSVLPSVAERWLAPRLPRFAAAHPHIAIALHPDAALAPLRKRDRIDVALRYGPGTWP
GVVAEKLMNETVFPVASPAYRNRDGIAPRTPADLVRATLLRHPAQPWEPWFQAARLDLVESARAPRFTDANALIDAALKG
RGVALARRSLIEPELAAGTLVRVSTVRVTDVYAHYVVWRPGHPHEAAIRTWLDWLRSEVRRRTPRR
>Mature_306_residues
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGHPLFTRERNGATPTHAAHALVLQVRQGLSV
LERAFEPALAVRDRPRTCDVTLNVSVLPSVAERWLAPRLPRFAAAHPHIAIALHPDAALAPLRKRDRIDVALRYGPGTWP
GVVAEKLMNETVFPVASPAYRNRDGIAPRTPADLVRATLLRHPAQPWEPWFQAARLDLVESARAPRFTDANALIDAALKG
RGVALARRSLIEPELAAGTLVRVSTVRVTDVYAHYVVWRPGHPHEAAIRTWLDWLRSEVRRRTPRR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=35.1351351351351, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1786448, Length=308, Percent_Identity=31.4935064935065, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=28.3333333333333, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI145693193, Length=272, Percent_Identity=30.5147058823529, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=26.530612244898, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787589, Length=300, Percent_Identity=26, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787128, Length=302, Percent_Identity=24.5033112582781, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788297, Length=170, Percent_Identity=28.8235294117647, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34161; Mature: 34161

Theoretical pI: Translated: 11.84; Mature: 11.84

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGHPLFTRERNG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCC
ATPTHAAHALVLQVRQGLSVLERAFEPALAVRDRPRTCDVTLNVSVLPSVAERWLAPRLP
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEEEHHHHHHHHHHHHCCCCC
RFAAAHPHIAIALHPDAALAPLRKRDRIDVALRYGPGTWPGVVAEKLMNETVFPVASPAY
HHHCCCCCEEEEECCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCC
RNRDGIAPRTPADLVRATLLRHPAQPWEPWFQAARLDLVESARAPRFTDANALIDAALKG
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
RGVALARRSLIEPELAAGTLVRVSTVRVTDVYAHYVVWRPGHPHEAAIRTWLDWLRSEVR
CCHHHHHHHHCCCHHHCCCEEEEEEEEHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
RRTPRR
HCCCCC
>Mature Secondary Structure
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGHPLFTRERNG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCC
ATPTHAAHALVLQVRQGLSVLERAFEPALAVRDRPRTCDVTLNVSVLPSVAERWLAPRLP
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEEEHHHHHHHHHHHHCCCCC
RFAAAHPHIAIALHPDAALAPLRKRDRIDVALRYGPGTWPGVVAEKLMNETVFPVASPAY
HHHCCCCCEEEEECCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCC
RNRDGIAPRTPADLVRATLLRHPAQPWEPWFQAARLDLVESARAPRFTDANALIDAALKG
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
RGVALARRSLIEPELAAGTLVRVSTVRVTDVYAHYVVWRPGHPHEAAIRTWLDWLRSEVR
CCHHHHHHHHCCCHHHCCCEEEEEEEEHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
RRTPRR
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]