Click here to switch to the map view.

The map label for this gene is proP [H]

Identifier: 115352753

GI number: 115352753

Start: 2976681

End: 2978339

Strand: Direct

Name: proP [H]

Synonym: Bamb_2702

Alternate gene names: 115352753

Gene position: 2976681-2978339 (Clockwise)

Preceding gene: 115352752

Following gene: 115352756

Centisome position: 83.7

GC content: 65.04

Gene sequence:

>1659_bases
ATGGCAACCTTAGGCGGGCAAATTTCGCACTCGCCGATGACGGCCGAAGAAAAGAAGGTAATCTTCGCGTCGTCGCTCGG
CACCGTGTTCGAGTGGTACGACTTTTACCTGGCCGGGTCACTCGCGGCCTACATCAGCAAGAGCTTCTTCTCCGGGGTCA
ATCCGACCGCCGCCTTCATCTTCACGCTGCTCGGCTTCGCGGCCGGCTTCGCCGTGCGACCGTTCGGCGCGATCGTGTTC
GGCCGGCTCGGCGACCTCGTCGGCCGCAAGCACACGTTCCTCGTGACGATCGTGATCATGGGCATCTCGACCTTCGTGGT
CGGCTTCCTGCCCGGCTACACGACGATCGGCATCGCGGCGCCCGTGATCTTCATCGCGATGCGGCTGCTGCAGGGCCTCG
CGCTCGGCGGCGAGTACGGCGGCGCGGCGACCTACGTCGCCGAGCATGCACCGGCGAACCGGCGCGGCTTCTACACCGCG
TGGATCCAGACGACCGCGACGCTCGGCCTGTTCCTGTCGCTGCTCGTGATCCTCGGCGTGCGCACGCTCATCGGCGAGGA
ATCGTTCGGCAACTGGGGCTGGCGCGTGCCGTTCGTCGCGTCGATCCTGCTGCTCGCGGTGTCGGTGTGGATCCGGTTGC
AGCTGAACGAATCGCCGGTGTTCCTGCGCATCAAGGCCGAGGGCAAGACGTCGAAGGCGCCGCTGACCGAAGCGTTCGGC
CAGTGGAAGAACCTGAAGATCGTGATCCTCGCGCTCGTCGGCCTGACCGCCGGCCAGGCCGTCGTCTGGTACACGGGCCA
GTTCTACGCGCTGTTCTTCCTCACGCAGACGCTGAAGGTCGACGGTGCCAGCGCGAACATCCTGATCGCGATCGCGCTCC
TGATCGGTACGCCGTTCTTCCTGTTCTTCGGCTCGCTGTCGGACCGGATCGGCCGCAAGCCGATCATCCTCGCCGGCTGC
CTGATCGCGGCGCTCACCTACTTCCCGCTGTTCAAGGCGCTCACGCACTACGCGAACCCGCAGCTCGAACTCGCGACGCA
GAAGGCACCGATCTCGGTCATCGCCGATCCGGCCACCTGCTCGTTCCAGTTCAACCCGGTGGGCACGTCGAAGTTCACGA
GCTCGTGCGACATCGTGAAGAGCGCGCTCGCAAAGGCCGGCCTGAACTACGAGAACGTCGCGGCGCCGGCAGGCTCGACC
GCGCAGATCAAGGTGGGCGACACGGTGATCCCGGCCTATGACGGCAAGGCGCCCGATGCGAAGGCGCAGGGCGCGGCGTT
TGACAAGACGCTCGCGTCGACGCTGAAGGGCGCCGGCTATCCGGCCAAGGCCGACCCGGCGCAGCTCAACTGGCCGATGA
CGATCGTGATACTGACGATCCTCGTGATCTATGTGACGATGGTCTACGGCCCGATCGCCGCGATGCTGGTCGAGATGTTC
CCGACGCGGATCCGCTATACGTCGATGTCGCTGCCCTATCACATCGGCAACGGCTGGTTCGGCGGCTTCCTGCCGGCGAC
CGCGTTCGCGATCGTCGCGGCGAAGGGCAACATCTATTCGGGGCTCTGGTATCCGATCGTGATCGCGCTGGCCACGTTCG
TGATCGGTCTGCTGTTCGTCAAGGAGACGAAGGACTCGAACATCTACGCGCAGGATTGA

Upstream 100 bases:

>100_bases
CGCAAGTCAGTGCGCCGTAAGTTTCCGTGCCAATAATCGTCGACAGGCCCGCCCACCCAAGGCGGCCGCATATTTATATC
AAGGGACTTGGAGACGATTC

Downstream 100 bases:

>100_bases
TGAGGTCGGGCAGGTGGGGGGCACCCCGCCTGCAACCCGGGCCGCCGGCAACTTTTTTCGGGGAAGATGAAAAAGGTGTT
GACAGCCCGGCGGCACATCT

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MATLGGQISHSPMTAEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVF
GRLGDLVGRKHTFLVTIVIMGISTFVVGFLPGYTTIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTA
WIQTTATLGLFLSLLVILGVRTLIGEESFGNWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFG
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDRIGRKPIILAGC
LIAALTYFPLFKALTHYANPQLELATQKAPISVIADPATCSFQFNPVGTSKFTSSCDIVKSALAKAGLNYENVAAPAGST
AQIKVGDTVIPAYDGKAPDAKAQGAAFDKTLASTLKGAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIVIALATFVIGLLFVKETKDSNIYAQD

Sequences:

>Translated_552_residues
MATLGGQISHSPMTAEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVF
GRLGDLVGRKHTFLVTIVIMGISTFVVGFLPGYTTIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTA
WIQTTATLGLFLSLLVILGVRTLIGEESFGNWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFG
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDRIGRKPIILAGC
LIAALTYFPLFKALTHYANPQLELATQKAPISVIADPATCSFQFNPVGTSKFTSSCDIVKSALAKAGLNYENVAAPAGST
AQIKVGDTVIPAYDGKAPDAKAQGAAFDKTLASTLKGAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIVIALATFVIGLLFVKETKDSNIYAQD
>Mature_551_residues
ATLGGQISHSPMTAEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVFG
RLGDLVGRKHTFLVTIVIMGISTFVVGFLPGYTTIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAW
IQTTATLGLFLSLLVILGVRTLIGEESFGNWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFGQ
WKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDRIGRKPIILAGCL
IAALTYFPLFKALTHYANPQLELATQKAPISVIADPATCSFQFNPVGTSKFTSSCDIVKSALAKAGLNYENVAAPAGSTA
QIKVGDTVIPAYDGKAPDAKAQGAAFDKTLASTLKGAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMFP
TRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIVIALATFVIGLLFVKETKDSNIYAQD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=325, Percent_Identity=36, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788292, Length=328, Percent_Identity=35.0609756097561, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1788942, Length=339, Percent_Identity=33.6283185840708, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789941, Length=220, Percent_Identity=43.1818181818182, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI87082231, Length=226, Percent_Identity=26.9911504424779, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082404, Length=325, Percent_Identity=24.9230769230769, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59094; Mature: 58963

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLGGQISHSPMTAEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFI
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FTLLGFAAGFAVRPFGAIVFGRLGDLVGRKHTFLVTIVIMGISTFVVGFLPGYTTIGIAA
HHHHHHHHCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
PVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAWIQTTATLGLFLSLLVILGV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
RTLIGEESFGNWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFG
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCCCHHHHHC
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFF
CCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCHHH
LFFGSLSDRIGRKPIILAGCLIAALTYFPLFKALTHYANPQLELATQKAPISVIADPATC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEE
SFQFNPVGTSKFTSSCDIVKSALAKAGLNYENVAAPAGSTAQIKVGDTVIPAYDGKAPDA
EEEECCCCCCHHCCHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEECCEEEECCCCCCCCC
KAQGAAFDKTLASTLKGAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMF
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIVIALATFVIGLLFV
HHHEEEEEECCCEEECCCCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHH
KETKDSNIYAQD
EECCCCCCEECC
>Mature Secondary Structure 
ATLGGQISHSPMTAEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFI
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FTLLGFAAGFAVRPFGAIVFGRLGDLVGRKHTFLVTIVIMGISTFVVGFLPGYTTIGIAA
HHHHHHHHCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
PVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAWIQTTATLGLFLSLLVILGV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
RTLIGEESFGNWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFG
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCCCHHHHHC
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFF
CCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCHHH
LFFGSLSDRIGRKPIILAGCLIAALTYFPLFKALTHYANPQLELATQKAPISVIADPATC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEE
SFQFNPVGTSKFTSSCDIVKSALAKAGLNYENVAAPAGSTAQIKVGDTVIPAYDGKAPDA
EEEECCCCCCHHCCHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEECCEEEECCCCCCCCC
KAQGAAFDKTLASTLKGAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMF
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIVIALATFVIGLLFV
HHHEEEEEECCCEEECCCCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHH
KETKDSNIYAQD
EECCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]