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The map label for this gene is xthA [H]

Identifier: 115352233

GI number: 115352233

Start: 2400486

End: 2401301

Strand: Direct

Name: xthA [H]

Synonym: Bamb_2182

Alternate gene names: 115352233

Gene position: 2400486-2401301 (Clockwise)

Preceding gene: 115352232

Following gene: 115352238

Centisome position: 67.49

GC content: 65.81

Gene sequence:

>816_bases
ATGGCGGCCTTCGCATTCACCCCGCACGCATTCCCGTTCATGAAAATCGCCACCTGGAACGTCAACTCGCTCAACGTCCG
CAAGCAGCACGTGCTCGACTGGATCGCGCACAGCGGAACCGACGTGCTGTGCCTGCAGGAACTGAAGCTGCCGGATGAGA
AGTATCCGCGCGCCGATCTCGAGGCCATCGGCTACCGCAGCTGGTTCACCGGCCAGCGGACCTACAACGGCGTCGCGATC
CTCGCGCGCGACACGCTCGCCGTCGACGAATCGGACATCGTGCGCAACATCCCCGGCTTCGACGATCCGCAGCAGCGCGT
GATCGCCGTGACGGTCGAAGGCGTGCGGATCGTGTCCGCCTATTTCCCGAACGGCCAGTCGCCCGAGTCCGACAAGTTCG
TCTACAAGATGCAGTGGCTCGACGCACTGCATGCGTGGCTGAGCGCCGAGATGCAGCGCTATCCGAAGCTCGCGCTGCTC
GGCGACTACAACATCGCGCCGGAAGACCGCGACGTGCACGATCCCGCGAAATGGGAAGGCCAGAACCTCGTGTCGCCGCA
GGAGCGCGCGCACTTCGCGAAGCTGCTCGAGCTCGGCTTCGTCGACGCGTTCCGCCGTTTCGAGCAGCCCGAGAAGACCT
TCACGTGGTGGGACTACCGGATGCTCGCGTTCCGCCGCAACGCGGGGCTGCGCATCGATCACATCCTGCTGTCGCCGGCG
CTCGCCGCCAACTGCACGTCGTGCGAAGTCGACCGCCTGCCGCGCACGTGGGAACAGCCGTCCGACCACACGCCCGTCGT
CGCGGTCGTCTGCTGA

Upstream 100 bases:

>100_bases
ACCTTTGTCACAATTTCTTCGCGGGCCGTCGATATCGCGATGCGCGGTGCGTGCTTCGCATCGCGTGTCGCGCGCGTTCG
CGCCCCGTGCTCGCGGTAAG

Downstream 100 bases:

>100_bases
GCCCCCCTTCCCGCTCGTGCCCGCGCTCAACCGCGTGCGGGCGCGGGCGCGGGCCCGAGATGGGCCCACAGGAAGCCGAA
TTCTGCCGCATCCGCTTCGG

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MAAFAFTPHAFPFMKIATWNVNSLNVRKQHVLDWIAHSGTDVLCLQELKLPDEKYPRADLEAIGYRSWFTGQRTYNGVAI
LARDTLAVDESDIVRNIPGFDDPQQRVIAVTVEGVRIVSAYFPNGQSPESDKFVYKMQWLDALHAWLSAEMQRYPKLALL
GDYNIAPEDRDVHDPAKWEGQNLVSPQERAHFAKLLELGFVDAFRRFEQPEKTFTWWDYRMLAFRRNAGLRIDHILLSPA
LAANCTSCEVDRLPRTWEQPSDHTPVVAVVC

Sequences:

>Translated_271_residues
MAAFAFTPHAFPFMKIATWNVNSLNVRKQHVLDWIAHSGTDVLCLQELKLPDEKYPRADLEAIGYRSWFTGQRTYNGVAI
LARDTLAVDESDIVRNIPGFDDPQQRVIAVTVEGVRIVSAYFPNGQSPESDKFVYKMQWLDALHAWLSAEMQRYPKLALL
GDYNIAPEDRDVHDPAKWEGQNLVSPQERAHFAKLLELGFVDAFRRFEQPEKTFTWWDYRMLAFRRNAGLRIDHILLSPA
LAANCTSCEVDRLPRTWEQPSDHTPVVAVVC
>Mature_270_residues
AAFAFTPHAFPFMKIATWNVNSLNVRKQHVLDWIAHSGTDVLCLQELKLPDEKYPRADLEAIGYRSWFTGQRTYNGVAIL
ARDTLAVDESDIVRNIPGFDDPQQRVIAVTVEGVRIVSAYFPNGQSPESDKFVYKMQWLDALHAWLSAEMQRYPKLALLG
DYNIAPEDRDVHDPAKWEGQNLVSPQERAHFAKLLELGFVDAFRRFEQPEKTFTWWDYRMLAFRRNAGLRIDHILLSPAL
AANCTSCEVDRLPRTWEQPSDHTPVVAVVC

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=261, Percent_Identity=30.2681992337165, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI18375503, Length=261, Percent_Identity=30.2681992337165, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI18375501, Length=261, Percent_Identity=30.2681992337165, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1788046, Length=269, Percent_Identity=33.457249070632, Blast_Score=148, Evalue=5e-37,
Organism=Drosophila melanogaster, GI221330655, Length=271, Percent_Identity=28.4132841328413, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17136678, Length=271, Percent_Identity=28.4132841328413, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31075; Mature: 30943

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFAFTPHAFPFMKIATWNVNSLNVRKQHVLDWIAHSGTDVLCLQELKLPDEKYPRADL
CCCEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCH
EAIGYRSWFTGQRTYNGVAILARDTLAVDESDIVRNIPGFDDPQQRVIAVTVEGVRIVSA
HHHCCHHEECCCCCCCCEEEEEECCEECCHHHHHHCCCCCCCCCCEEEEEEECCEEEEEE
YFPNGQSPESDKFVYKMQWLDALHAWLSAEMQRYPKLALLGDYNIAPEDRDVHDPAKWEG
ECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCC
QNLVSPQERAHFAKLLELGFVDAFRRFEQPEKTFTWWDYRMLAFRRNAGLRIDHILLSPA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHEEEEEEECCCCCEEEHEECCHH
LAANCTSCEVDRLPRTWEQPSDHTPVVAVVC
HHCCCCCCCHHCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
AAFAFTPHAFPFMKIATWNVNSLNVRKQHVLDWIAHSGTDVLCLQELKLPDEKYPRADL
CCEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCH
EAIGYRSWFTGQRTYNGVAILARDTLAVDESDIVRNIPGFDDPQQRVIAVTVEGVRIVSA
HHHCCHHEECCCCCCCCEEEEEECCEECCHHHHHHCCCCCCCCCCEEEEEEECCEEEEEE
YFPNGQSPESDKFVYKMQWLDALHAWLSAEMQRYPKLALLGDYNIAPEDRDVHDPAKWEG
ECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCC
QNLVSPQERAHFAKLLELGFVDAFRRFEQPEKTFTWWDYRMLAFRRNAGLRIDHILLSPA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHEEEEEEECCCCCEEEHEECCHH
LAANCTSCEVDRLPRTWEQPSDHTPVVAVVC
HHCCCCCCCHHCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]