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The map label for this gene is cls

Identifier: 115352117

GI number: 115352117

Start: 2267147

End: 2268586

Strand: Direct

Name: cls

Synonym: Bamb_2066

Alternate gene names: 115352117

Gene position: 2267147-2268586 (Clockwise)

Preceding gene: 115352116

Following gene: 115352119

Centisome position: 63.75

GC content: 67.36

Gene sequence:

>1440_bases
ATGACCCTCGACTGGCTACATCTCGGCACCCTGATCCTGGCCGTTCACGTGCTCGGGATCGTCGCGGCGTGTCACGCGCT
CATGAACACGCGCACGTCGCAAGGCGCGATCGCGTGGGCCGTGTCGCTCGCCGCGATGCCGTACCTGACGCTCGTCCCCT
ACCTGTTCCTCGGTCGCAGCAAGTTCTCCGGCTACGTCGACGCGCGGCGCCACGAAATGGAAGCGTTGCGCACGCATACG
CCGCGCGTGCCGTGGCTCGATGCGGACGCAACCAACGGGCCGGCGGCCGACGCGATCGGCCGCGCTGCGGTGCTCGCGCT
CACCCGGCTCGGCGGGATGCCGTTCGTCGGCGGCAATTCGGTGCGCACGCTCGTCAACGGCGATGCGACGTTCGAGGCGA
TCCTGTCGGCGATCGACTCGGCGCGCGAATACGTGTTCGTCCAGTTCTTCATCGTGCGCGTCGACGCGCTCGGCCTGATG
CTGCGCGACGCGCTGCTCGCCCGCGCGGCAGCCGGCGTGCGCTGCTACCTGCTGTATGACAGCATCGGCTGCTTCGACCT
GCCGCACAGCTACGTCGACACGCTGCGCCGCGGCGGCGTCGAGGTCCACCCGTTCGCGACCCACCGCAAGTTCGTCAACC
GCTTCCAGCTCAACTTCCGCAATCATCGCAAGATCGTCGTCGTCGACGGCAATCGCGCGTTCGTCGGCGGACACAACGTC
GGCGTCGAATATCTCGGCGCGAACCGGCGTCTGTCGCCGTGGCGCGACACCCATATCGAAATCCGCGGCCCGGTGGTGGC
CAGCATTCAGTACGTGTTCGCCGAAGACTGGCACTGGGCCACGCAGACGCTTCCGCCGCTCGCCGCGCCGCCGGAGGCGC
TGCCCGGCGACGCGATGCATTGCCTCGCCGTACCGATGGGCCCGGCCGACAAGCAGGAAACCGGCTCGCTGTTCTTCGTC
GGCGCGATCAACGCCGCGCACGAACGGGTGTGGATCACGACGCCGTATCTCATACCCGACGAAGCGGTGATCGCCGCGCT
GAAGCTCGCGGTGATGCGCGGCGTCGATGTGCGCATCCTGATCCCGTGCCGGCGCGACCACTACGTCGTGTTCGAGGCGT
CGAAGCTCTATGCGCGCGATCTCATCGACGCAGGCGTCAAGGTGTTCCGCTACCGCCCGGGCTTCCTGCACCAGAAAGTC
GTGCTGATCGATCGCATCGCGGCCGCGGTCGGCAGCGCGAATCTCGACAACCGCTCGTTCCGGCTGAACTTCGAAATCAT
GGTGCTGACCGTCGATCGCGCGTTCGCCGATGAAGTCGGGGCCATGCTCGACGCCGATTTCGCGCAGTCCTACGAGGTCG
ATGCGAACGAGTATCGGCACTCGCCCGCGTGGCGGCGCATCGCCATGCACGTCGCGCGCCTGTTCGCACCCATCCTGTAG

Upstream 100 bases:

>100_bases
CCCAACCGTGCGGCACGGCAAACCGCTGTCCCGCACGGTTGGGTAAGATGCGCGTAAGCGGCCGTCGCGCCGCACGACTT
CAGGAGTCCGTCACGCGCCC

Downstream 100 bases:

>100_bases
CGTCCGTGGCGCCCGCGCGAGCGCCGGGCCGCCGTCGCCCGGATGGCGCCGCCCGCTTTCGCCCCATCCGGCCCGACACC
GCGGTCGCGGGCCAGACGCC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTLDWLHLGTLILAVHVLGIVAACHALMNTRTSQGAIAWAVSLAAMPYLTLVPYLFLGRSKFSGYVDARRHEMEALRTHT
PRVPWLDADATNGPAADAIGRAAVLALTRLGGMPFVGGNSVRTLVNGDATFEAILSAIDSAREYVFVQFFIVRVDALGLM
LRDALLARAAAGVRCYLLYDSIGCFDLPHSYVDTLRRGGVEVHPFATHRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNV
GVEYLGANRRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQTLPPLAAPPEALPGDAMHCLAVPMGPADKQETGSLFFV
GAINAAHERVWITTPYLIPDEAVIAALKLAVMRGVDVRILIPCRRDHYVVFEASKLYARDLIDAGVKVFRYRPGFLHQKV
VLIDRIAAAVGSANLDNRSFRLNFEIMVLTVDRAFADEVGAMLDADFAQSYEVDANEYRHSPAWRRIAMHVARLFAPIL

Sequences:

>Translated_479_residues
MTLDWLHLGTLILAVHVLGIVAACHALMNTRTSQGAIAWAVSLAAMPYLTLVPYLFLGRSKFSGYVDARRHEMEALRTHT
PRVPWLDADATNGPAADAIGRAAVLALTRLGGMPFVGGNSVRTLVNGDATFEAILSAIDSAREYVFVQFFIVRVDALGLM
LRDALLARAAAGVRCYLLYDSIGCFDLPHSYVDTLRRGGVEVHPFATHRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNV
GVEYLGANRRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQTLPPLAAPPEALPGDAMHCLAVPMGPADKQETGSLFFV
GAINAAHERVWITTPYLIPDEAVIAALKLAVMRGVDVRILIPCRRDHYVVFEASKLYARDLIDAGVKVFRYRPGFLHQKV
VLIDRIAAAVGSANLDNRSFRLNFEIMVLTVDRAFADEVGAMLDADFAQSYEVDANEYRHSPAWRRIAMHVARLFAPIL
>Mature_478_residues
TLDWLHLGTLILAVHVLGIVAACHALMNTRTSQGAIAWAVSLAAMPYLTLVPYLFLGRSKFSGYVDARRHEMEALRTHTP
RVPWLDADATNGPAADAIGRAAVLALTRLGGMPFVGGNSVRTLVNGDATFEAILSAIDSAREYVFVQFFIVRVDALGLML
RDALLARAAAGVRCYLLYDSIGCFDLPHSYVDTLRRGGVEVHPFATHRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNVG
VEYLGANRRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQTLPPLAAPPEALPGDAMHCLAVPMGPADKQETGSLFFVG
AINAAHERVWITTPYLIPDEAVIAALKLAVMRGVDVRILIPCRRDHYVVFEASKLYARDLIDAGVKVFRYRPGFLHQKVV
LIDRIAAAVGSANLDNRSFRLNFEIMVLTVDRAFADEVGAMLDADFAQSYEVDANEYRHSPAWRRIAMHVARLFAPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=472, Percent_Identity=29.0254237288136, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1787007, Length=352, Percent_Identity=30.9659090909091, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 53059; Mature: 52928

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDWLHLGTLILAVHVLGIVAACHALMNTRTSQGAIAWAVSLAAMPYLTLVPYLFLGRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
KFSGYVDARRHEMEALRTHTPRVPWLDADATNGPAADAIGRAAVLALTRLGGMPFVGGNS
CCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCC
VRTLVNGDATFEAILSAIDSAREYVFVQFFIVRVDALGLMLRDALLARAAAGVRCYLLYD
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
SIGCFDLPHSYVDTLRRGGVEVHPFATHRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNV
CCCCCCCCHHHHHHHHHCCEEEEECHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCC
GVEYLGANRRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQTLPPLAAPPEALPGDAMH
CEEEECCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCCHHHCCCCCCCCCCCCCCCCEE
CLAVPMGPADKQETGSLFFVGAINAAHERVWITTPYLIPDEAVIAALKLAVMRGVDVRIL
EEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPCRRDHYVVFEASKLYARDLIDAGVKVFRYRPGFLHQKVVLIDRIAAAVGSANLDNRSF
EECCCCCEEEEECHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEE
RLNFEIMVLTVDRAFADEVGAMLDADFAQSYEVDANEYRHSPAWRRIAMHVARLFAPIL
EEEEEEEEEEECHHHHHHHHHHHCCHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLDWLHLGTLILAVHVLGIVAACHALMNTRTSQGAIAWAVSLAAMPYLTLVPYLFLGRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
KFSGYVDARRHEMEALRTHTPRVPWLDADATNGPAADAIGRAAVLALTRLGGMPFVGGNS
CCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCC
VRTLVNGDATFEAILSAIDSAREYVFVQFFIVRVDALGLMLRDALLARAAAGVRCYLLYD
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
SIGCFDLPHSYVDTLRRGGVEVHPFATHRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNV
CCCCCCCCHHHHHHHHHCCEEEEECHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCC
GVEYLGANRRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQTLPPLAAPPEALPGDAMH
CEEEECCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCCHHHCCCCCCCCCCCCCCCCEE
CLAVPMGPADKQETGSLFFVGAINAAHERVWITTPYLIPDEAVIAALKLAVMRGVDVRIL
EEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPCRRDHYVVFEASKLYARDLIDAGVKVFRYRPGFLHQKVVLIDRIAAAVGSANLDNRSF
EECCCCCEEEEECHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEE
RLNFEIMVLTVDRAFADEVGAMLDADFAQSYEVDANEYRHSPAWRRIAMHVARLFAPIL
EEEEEEEEEEECHHHHHHHHHHHCCHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA