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The map label for this gene is dapE [H]

Identifier: 115352115

GI number: 115352115

Start: 2264973

End: 2266112

Strand: Direct

Name: dapE [H]

Synonym: Bamb_2064

Alternate gene names: 115352115

Gene position: 2264973-2266112 (Clockwise)

Preceding gene: 115352114

Following gene: 115352116

Centisome position: 63.68

GC content: 69.12

Gene sequence:

>1140_bases
ATGTCCGCCACCCTAGCCCTTACCGAACAGCTGATCGCCCGCGCGTCCGTCACGCCCGACGACCAGCATTGCCAGCAGCT
CATGACCGAGCGCCTCGCCGCGCTCGGCTTCGAATGCGAGACCATCGCGTCGCACGGCGTGACCAACCTGTGGGCCGTCA
AGCGCGGCGCCGACGGCCGCGACGGCAAGCTGCTCGCGTTCGCGGGCCACACCGACGTCGTGCCGACCGGCCCGCTCGAG
CAGTGGACCTCGCCGCCGTTCGTCCCCACGCATCGCGACGGCAAGCTGTACGGCCGCGGCGCGGCCGACATGAAGACGTC
GCTCGCGGCGTTCGTCGTCGCGTCCGAGGAATTCGTCGCGGCCCACCCCGGCCACCGCGGCGCGATCGCGTTCCTGATCA
CGAGCGACGAGGAAGGCCCGGCCACCGACGGCACCGTGAAGGTCGTCGAACTGCTCGAAACGCGCGGCGAACGCGTCGAC
TACTGCATCGTCGGCGAGCCGACCTCGAGCGCGGAGCTCGGCGACGTCGTGAAGAACGGCCGCCGCGGCTCGATGTCGGG
CGAGCTGGTCGTCAAGGGCGTGCAGGGCCACATCGCATACCCGCACCTCGCGAAGAACCCGATCCACCTGCTCGCGCCGG
CGCTCGCCGAGCTCGCCGCCGAACAGTGGGACGCAGGCAACGAATACTTCCCGCCGACCACCTGGCAGGTGTCGAACCTG
CATGCCGGCACCGGCGCGACCAACGTGATCCCCGGCCATGCGGACCTGATGTTCAACTTCCGCTTTTCGACGGCGAGCAC
GGTCGAGGGCCTGCAGGCACGCGTGCATGCGATCCTCGGCAAGCACGGCCTCGAATACACGCTGAAGTGGTCGGTGAGCG
GCCTGCCGTTCCTCACGCCGCGCGGCGACCTGTCGAACGCGCTGGAGAACGCGATCCGCGCCGAGACCGGCCTCACGACC
GAGCTGTCGACGACGGGCGGCACGTCCGACGGACGCTTCATCGCGCGCATCTGCCCGCAGGTGATCGAGTTCGGCCCGCC
GAACGGCAGCATCCACAAGATCGACGAGCACATCGACGTGCGCTTCGTCGACCCGCTGAAGAACGTGTACCGACGCGTGC
TCGAACAACTGATCGCCTGA

Upstream 100 bases:

>100_bases
TGCCGCCTAGGGCCGCCCCCGCTGCGCCCGCGGGTGCAGCCCGACGTCTCGTCACTGCCGGCCACCGCGCCGGCATTTTT
TATCGAAAGTGGTCCGAACC

Downstream 100 bases:

>100_bases
CGGAGCCTCGCATGACGACACCATTTGCAACTATTCGCGACCTGCTGCGCTACGCGGTCACGCGCTTCTCGAAGGCCAAG
CTCGCATTCGGCCACGGCTC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSATLALTEQLIARASVTPDDQHCQQLMTERLAALGFECETIASHGVTNLWAVKRGADGRDGKLLAFAGHTDVVPTGPLE
QWTSPPFVPTHRDGKLYGRGAADMKTSLAAFVVASEEFVAAHPGHRGAIAFLITSDEEGPATDGTVKVVELLETRGERVD
YCIVGEPTSSAELGDVVKNGRRGSMSGELVVKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDAGNEYFPPTTWQVSNL
HAGTGATNVIPGHADLMFNFRFSTASTVEGLQARVHAILGKHGLEYTLKWSVSGLPFLTPRGDLSNALENAIRAETGLTT
ELSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIDVRFVDPLKNVYRRVLEQLIA

Sequences:

>Translated_379_residues
MSATLALTEQLIARASVTPDDQHCQQLMTERLAALGFECETIASHGVTNLWAVKRGADGRDGKLLAFAGHTDVVPTGPLE
QWTSPPFVPTHRDGKLYGRGAADMKTSLAAFVVASEEFVAAHPGHRGAIAFLITSDEEGPATDGTVKVVELLETRGERVD
YCIVGEPTSSAELGDVVKNGRRGSMSGELVVKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDAGNEYFPPTTWQVSNL
HAGTGATNVIPGHADLMFNFRFSTASTVEGLQARVHAILGKHGLEYTLKWSVSGLPFLTPRGDLSNALENAIRAETGLTT
ELSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIDVRFVDPLKNVYRRVLEQLIA
>Mature_378_residues
SATLALTEQLIARASVTPDDQHCQQLMTERLAALGFECETIASHGVTNLWAVKRGADGRDGKLLAFAGHTDVVPTGPLEQ
WTSPPFVPTHRDGKLYGRGAADMKTSLAAFVVASEEFVAAHPGHRGAIAFLITSDEEGPATDGTVKVVELLETRGERVDY
CIVGEPTSSAELGDVVKNGRRGSMSGELVVKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDAGNEYFPPTTWQVSNLH
AGTGATNVIPGHADLMFNFRFSTASTVEGLQARVHAILGKHGLEYTLKWSVSGLPFLTPRGDLSNALENAIRAETGLTTE
LSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIDVRFVDPLKNVYRRVLEQLIA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=376, Percent_Identity=56.9148936170213, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1790395, Length=399, Percent_Identity=26.0651629072682, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789236, Length=269, Percent_Identity=23.4200743494424, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 40694; Mature: 40563

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATLALTEQLIARASVTPDDQHCQQLMTERLAALGFECETIASHGVTNLWAVKRGADGR
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCC
DGKLLAFAGHTDVVPTGPLEQWTSPPFVPTHRDGKLYGRGAADMKTSLAAFVVASEEFVA
CCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCEECCCCHHHHHHHHHEEEECCHHEE
AHPGHRGAIAFLITSDEEGPATDGTVKVVELLETRGERVDYCIVGEPTSSAELGDVVKNG
ECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCC
RRGSMSGELVVKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDAGNEYFPPTTWQVSNL
CCCCCCCCEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCE
HAGTGATNVIPGHADLMFNFRFSTASTVEGLQARVHAILGKHGLEYTLKWSVSGLPFLTP
ECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEECC
RGDLSNALENAIRAETGLTTELSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIDV
CCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCE
RFVDPLKNVYRRVLEQLIA
EEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SATLALTEQLIARASVTPDDQHCQQLMTERLAALGFECETIASHGVTNLWAVKRGADGR
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCC
DGKLLAFAGHTDVVPTGPLEQWTSPPFVPTHRDGKLYGRGAADMKTSLAAFVVASEEFVA
CCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCEECCCCHHHHHHHHHEEEECCHHEE
AHPGHRGAIAFLITSDEEGPATDGTVKVVELLETRGERVDYCIVGEPTSSAELGDVVKNG
ECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCC
RRGSMSGELVVKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDAGNEYFPPTTWQVSNL
CCCCCCCCEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCE
HAGTGATNVIPGHADLMFNFRFSTASTVEGLQARVHAILGKHGLEYTLKWSVSGLPFLTP
ECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEECC
RGDLSNALENAIRAETGLTTELSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIDV
CCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCE
RFVDPLKNVYRRVLEQLIA
EEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA