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The map label for this gene is psdht [H]

Identifier: 115351985

GI number: 115351985

Start: 2131194

End: 2132249

Strand: Direct

Name: psdht [H]

Synonym: Bamb_1934

Alternate gene names: 115351985

Gene position: 2131194-2132249 (Clockwise)

Preceding gene: 115351984

Following gene: 115351987

Centisome position: 59.92

GC content: 68.94

Gene sequence:

>1056_bases
GTGCGGGCCGGCCCCACCCCCGCTACCCGCCACGTCATGTCGACTGAACAACAGGGCACCGCCCATACGACGATCGACGG
CGAGCCGATTCCGACGCTCGACGAAATCGCCGCGCAGCATTTCGCGTTGTCGCCGTGGGTCGCACGCACGCCGGTGTTCG
AGCGCGCCGATCTCCCATCCCTCGAGGGCACGCACGTCAACTTCAAGTTCGAGCTGCTGCAGGCGAGCGGCAGCTTCAAG
GCGCGCGGTGCGTTCACGCACCTGCTGGCACTCGACGAAGCGCGCAAGAACGCCGGCGTCACGTGCGTGTCGGCCGGCAA
TCATGCGGCGGCGGTCGCGTATGCGGCGATGCGGCTCGGCAGCAGCGCGAAGGTCGTGATGTTCCATACCGCGAGCCCGA
CGCGCATCGCGCAGTGCAAGCAGTACCGCGCGGAAGTCGTGCTCGCCGGCAGCGCGTCGCAGGCGTTCGACGTCGTGCGG
CGGATCGAAGCCGAGGAAGGCCGCTTCTTCATCCACCCGTTCAACGGTTACCACACGATTCTCGGCACCGCGACGCTCGG
CTACGAATGGGCGACGCAGACGCCCGATCTCGACGCGGTGATCGTGCCGATCGGCGGCGGCGGGCTCGCGGCCGGCATGG
CGACCGCGCTGCGGCTCGCGAATCCGCGCGTGCACGTGTACGGGGTCGAGCCGGAAGGCGCCGACGCGATGTGCCGCAGC
TTCGCGGCGAATCACACGATCAAGATGAGCGCGATGCAGACCATCGCCGATTCGCTGATGGCGCCGCACACCGAGGAATA
CAGCTACCAGCTGTGCCGGCGCCACATCGACCGCATCGTCACGGTGTCCGACGACGCGCTGCGCGCGGCGATGCTGTTCC
TGTTCTCGCACCTGAAGCTGGCGGTCGAACCGGCGTGCGCGGCGGCGCTCGCGGCGCTGCTCGGGCCGCTGCGCGAGACG
CTGCAGGGCAAGCGCGTCGGCGTGCTGCTGTCGGGCACGAATACCGACCCCGCGACACTCGGCATGCATCTCGCGCACGC
GAAACTGCAGCACTGA

Upstream 100 bases:

>100_bases
AGCTCGCGCAGCTCGCCGCCTGCGAGCAGTTCCTGCGCAAGTTCATCCGCAGCATGTCGGTCGACGCACACTGACTGCCC
CCCGCCACGCCGGCCTGCGT

Downstream 100 bases:

>100_bases
ACCTGCGCATGGCGCTGCGGGTTATCCCCAGTTTATGTTGACAGCCCTGTTGATAACCCGCCGGGCAAGCACGCAAGTGG
TTGAGCGCGCAGCGTTTTAC

Product: pyridoxal-5'-phosphate-dependent enzyme subunit beta

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MRAGPTPATRHVMSTEQQGTAHTTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFELLQASGSFK
ARGAFTHLLALDEARKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTASPTRIAQCKQYRAEVVLAGSASQAFDVVR
RIEAEEGRFFIHPFNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMCRS
FAANHTIKMSAMQTIADSLMAPHTEEYSYQLCRRHIDRIVTVSDDALRAAMLFLFSHLKLAVEPACAAALAALLGPLRET
LQGKRVGVLLSGTNTDPATLGMHLAHAKLQH

Sequences:

>Translated_351_residues
MRAGPTPATRHVMSTEQQGTAHTTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFELLQASGSFK
ARGAFTHLLALDEARKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTASPTRIAQCKQYRAEVVLAGSASQAFDVVR
RIEAEEGRFFIHPFNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMCRS
FAANHTIKMSAMQTIADSLMAPHTEEYSYQLCRRHIDRIVTVSDDALRAAMLFLFSHLKLAVEPACAAALAALLGPLRET
LQGKRVGVLLSGTNTDPATLGMHLAHAKLQH
>Mature_351_residues
MRAGPTPATRHVMSTEQQGTAHTTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFELLQASGSFK
ARGAFTHLLALDEARKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTASPTRIAQCKQYRAEVVLAGSASQAFDVVR
RIEAEEGRFFIHPFNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMCRS
FAANHTIKMSAMQTIADSLMAPHTEEYSYQLCRRHIDRIVTVSDDALRAAMLFLFSHLKLAVEPACAAALAALLGPLRET
LQGKRVGVLLSGTNTDPATLGMHLAHAKLQH

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=309, Percent_Identity=30.7443365695793, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI1789505, Length=271, Percent_Identity=38.3763837638376, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1790207, Length=304, Percent_Identity=31.9078947368421, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71991565, Length=308, Percent_Identity=35.7142857142857, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17508781, Length=319, Percent_Identity=29.4670846394984, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17537387, Length=308, Percent_Identity=32.1428571428571, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6322631, Length=309, Percent_Identity=34.6278317152104, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320930, Length=274, Percent_Identity=30.6569343065693, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319788, Length=284, Percent_Identity=23.943661971831, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355833, Length=311, Percent_Identity=35.6913183279743, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24645328, Length=219, Percent_Identity=35.1598173515982, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 37555; Mature: 37555

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAGPTPATRHVMSTEQQGTAHTTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPS
CCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHCCCCCCCCCCCC
LEGTHVNFKFELLQASGSFKARGAFTHLLALDEARKNAGVTCVSAGNHAAAVAYAAMRLG
CCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCC
SSAKVVMFHTASPTRIAQCKQYRAEVVLAGSASQAFDVVRRIEAEEGRFFIHPFNGYHTI
CCCEEEEEECCCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCEEE
LGTATLGYEWATQTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMCRS
EEEEEECEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
FAANHTIKMSAMQTIADSLMAPHTEEYSYQLCRRHIDRIVTVSDDALRAAMLFLFSHLKL
HHCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHH
AVEPACAAALAALLGPLRETLQGKRVGVLLSGTNTDPATLGMHLAHAKLQH
HHCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRAGPTPATRHVMSTEQQGTAHTTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPS
CCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHCCCCCCCCCCCC
LEGTHVNFKFELLQASGSFKARGAFTHLLALDEARKNAGVTCVSAGNHAAAVAYAAMRLG
CCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCC
SSAKVVMFHTASPTRIAQCKQYRAEVVLAGSASQAFDVVRRIEAEEGRFFIHPFNGYHTI
CCCEEEEEECCCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCEEE
LGTATLGYEWATQTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMCRS
EEEEEECEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
FAANHTIKMSAMQTIADSLMAPHTEEYSYQLCRRHIDRIVTVSDDALRAAMLFLFSHLKL
HHCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHH
AVEPACAAALAALLGPLRETLQGKRVGVLLSGTNTDPATLGMHLAHAKLQH
HHCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {L-Ser}} 5 {L-Ser}} 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]