Click here to switch to the map view.

The map label for this gene is cysW [H]

Identifier: 115351552

GI number: 115351552

Start: 1642526

End: 1643473

Strand: Direct

Name: cysW [H]

Synonym: Bamb_1499

Alternate gene names: 115351552

Gene position: 1642526-1643473 (Clockwise)

Preceding gene: 115351551

Following gene: 115351553

Centisome position: 46.18

GC content: 68.04

Gene sequence:

>948_bases
ATGAGCCAGGAGTCCAGTGCCGTGCTGAACACCCCGTCCGCCGCGCGCGCGCGTGCCGCGAAACGGCCCGACCCCGTCAG
CGAGTCGCGCGTCGTGCGCTGGCTGCTCACGGGCATCGCGCTGGCGTTCCTCGCGTTCTTCCTCGTCGTGCCGCTCGCCG
CGGTGTTCTTCGAGGCGCTGCGCAAGGGCGTCGGCTTCTATCTCGAATCGCTCGCCGATCCGGACGCATGGTCGGCGATC
AAGCTGACGCTGACGGTCGCCGTGATCGCGGTGCCGCTGAACCTCGTGTTCGGCGTGTGCGCGTCGTGGGCGATCGCGAA
ATTCGAATTTCGCGGCAAGGCATTGCTGACCACGCTGATCGACCTGCCGTTCTCCGTGTCGCCGGTCATCTCGGGCCTCG
TCTACGTGCTGCTGTTCGGCGCGCAGGGCTGGCTCGGGCCGTGGCTGCAGGACCACGACGTGCAGATCATCTTCGCGGTG
CCGGGCATCGTGCTCGCGACGATCTTCGTCACGTTCCCGTTCGTCGCGCGCGAGCTGATCCCGCTGATGCAGGCGCAGGG
CACCGACGAGGAAGAGGCCGCGCGCGTGCTCGGTGCGTCGGGCTGGCAGATCTTCCGGCGCGTGACGCTGCCGAACGTGA
AGTGGGGCCTGCTGTACGGCGTGATCCTGTGCAATGCGCGCGCGATGGGCGAGTTCGGTGCGGTGTCGGTCGTGTCCGGC
CACATTCGCGGCGTGACCGACACGATGCCGCTGCACGTCGAGATCCTGTACAACGAATACAACTTCGCGGCGGCGTTCGC
GGTGGCGTCGGTGCTGGCGCTGCTCGCGCTCGTCACGCTCGCGCTGAAGCTGCTCGCCGAGCGTCATCTCGCCGCCGAAC
TGGCCGGCGCACGCGATGACGTTCCCGCTCATGCCGGCCCCGTCGCCGCCATTTCGTCGAAATCGTAA

Upstream 100 bases:

>100_bases
ACACGCTGCAGTGGTATCTGCAGCGTCGTACGAGCAAGGGGGCGAGCGGCCCCGCGCCGTCGAGCGCAACCATTGCCGCG
ACCGTCCAGGGAGGCCAGCA

Downstream 100 bases:

>100_bases
AGAGGCAACCAGAATGGGTATCACCGTCCGTCACCTTCAAAAGCGCTTCGGCGATTTCACCGCCCTCGACAACGTGTCGC
TCGACTTCCCCGCGGGCGAG

Product: sulfate ABC transporter inner membrane subunit CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSQESSAVLNTPSAARARAAKRPDPVSESRVVRWLLTGIALAFLAFFLVVPLAAVFFEALRKGVGFYLESLADPDAWSAI
KLTLTVAVIAVPLNLVFGVCASWAIAKFEFRGKALLTTLIDLPFSVSPVISGLVYVLLFGAQGWLGPWLQDHDVQIIFAV
PGIVLATIFVTFPFVARELIPLMQAQGTDEEEAARVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVSG
HIRGVTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLLAERHLAAELAGARDDVPAHAGPVAAISSKS

Sequences:

>Translated_315_residues
MSQESSAVLNTPSAARARAAKRPDPVSESRVVRWLLTGIALAFLAFFLVVPLAAVFFEALRKGVGFYLESLADPDAWSAI
KLTLTVAVIAVPLNLVFGVCASWAIAKFEFRGKALLTTLIDLPFSVSPVISGLVYVLLFGAQGWLGPWLQDHDVQIIFAV
PGIVLATIFVTFPFVARELIPLMQAQGTDEEEAARVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVSG
HIRGVTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLLAERHLAAELAGARDDVPAHAGPVAAISSKS
>Mature_314_residues
SQESSAVLNTPSAARARAAKRPDPVSESRVVRWLLTGIALAFLAFFLVVPLAAVFFEALRKGVGFYLESLADPDAWSAIK
LTLTVAVIAVPLNLVFGVCASWAIAKFEFRGKALLTTLIDLPFSVSPVISGLVYVLLFGAQGWLGPWLQDHDVQIIFAVP
GIVLATIFVTFPFVARELIPLMQAQGTDEEEAARVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVSGH
IRGVTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLLAERHLAAELAGARDDVPAHAGPVAAISSKS

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=268, Percent_Identity=53.7313432835821, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=37.378640776699, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1786980, Length=181, Percent_Identity=35.9116022099448, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787369, Length=175, Percent_Identity=27.4285714285714, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33708; Mature: 33577

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQESSAVLNTPSAARARAAKRPDPVSESRVVRWLLTGIALAFLAFFLVVPLAAVFFEAL
CCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKGVGFYLESLADPDAWSAIKLTLTVAVIAVPLNLVFGVCASWAIAKFEFRGKALLTTLI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
DLPFSVSPVISGLVYVLLFGAQGWLGPWLQDHDVQIIFAVPGIVLATIFVTFPFVARELI
HCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
PLMQAQGTDEEEAARVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVSG
HHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCCCCHHHHHC
HIRGVTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLLAERHLAAELAGARDD
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPAHAGPVAAISSKS
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQESSAVLNTPSAARARAAKRPDPVSESRVVRWLLTGIALAFLAFFLVVPLAAVFFEAL
CCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKGVGFYLESLADPDAWSAIKLTLTVAVIAVPLNLVFGVCASWAIAKFEFRGKALLTTLI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
DLPFSVSPVISGLVYVLLFGAQGWLGPWLQDHDVQIIFAVPGIVLATIFVTFPFVARELI
HCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
PLMQAQGTDEEEAARVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVSG
HHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCCCCHHHHHC
HIRGVTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLLAERHLAAELAGARDD
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPAHAGPVAAISSKS
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]