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The map label for this gene is rlmE [H]

Identifier: 115351226

GI number: 115351226

Start: 1282899

End: 1283561

Strand: Direct

Name: rlmE [H]

Synonym: Bamb_1173

Alternate gene names: 115351226

Gene position: 1282899-1283561 (Clockwise)

Preceding gene: 115351224

Following gene: 115351227

Centisome position: 36.07

GC content: 62.44

Gene sequence:

>663_bases
ATGGCAAAAAACCGCTTCAACCAGAACTGGCTGCACGACCACATCAACGACCCGTACGTCAAAATGGCGCAGCGGGAGGG
CTATCGCGCGCGTGCCGCGTACAAGCTGAAGGAAATCGACGAGCAGGACAAGCTGATCCGTCCGGGCCAGGTGATCGTCG
ATCTCGGCGCGACGCCGGGCAGCTGGAGCCAGTATGCCCGCAACAAGCTCGCGCAGGGCAAGAAGCGCGACACGCAACGC
GAAGGCGGCATCGACGGCACGATCATCGCGCTCGACCTGCTGCCGATGGAGCCGATCGCCGACGTCCACTTCATCCAGGG
CGACTTCCGCGAGGACGACGTCCTCCGGCAGCTCGAAGATGTGCTCGAAGGTCGCGCGGTGGACCTTGTTATTTCCGACA
TGGCCCCCAACCTCTCCGGCGTAGCCTCGGCGGACGCAGCACGCATCGAGCATCTGTGCGATCTGGCGCTTGAATTCGCG
CAGAACCATCTGAAGCCGGATGGTGCGCTGCTCGTGAAGTGCTTTCACGGCAGCGGCTACAGCCAGATCGTCGAGAAGTT
CAAGCAGCAGTTTAAGGTGGTCGCGCCCCGCAAGCCGAAGGCGTCGCGCGACAAATCGTCCGAAACGTTCATTTTGGGCC
GGCAGCTGAAGCAACCGCGGTGA

Upstream 100 bases:

>100_bases
GGCTTGGGCCTTGAAAGGGGCCGCAGCCGCGCGAAGAAGACGCGTATTATCCGCCAAAAGCACGGCTTCACGAAGCAAAT
AGCAGCAATCTCTCTCTCGA

Downstream 100 bases:

>100_bases
CGCGGAGCGCGGTGCCGCAAACGGGCTCCGGCCCTTGCCAGACAAGCGAAGCGCTATCGCGGCGGTCGTCAATGCTTATG
GCAGGGGTGGTCGGACTGGA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKNRFNQNWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPGSWSQYARNKLAQGKKRDTQR
EGGIDGTIIALDLLPMEPIADVHFIQGDFREDDVLRQLEDVLEGRAVDLVISDMAPNLSGVASADAARIEHLCDLALEFA
QNHLKPDGALLVKCFHGSGYSQIVEKFKQQFKVVAPRKPKASRDKSSETFILGRQLKQPR

Sequences:

>Translated_220_residues
MAKNRFNQNWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPGSWSQYARNKLAQGKKRDTQR
EGGIDGTIIALDLLPMEPIADVHFIQGDFREDDVLRQLEDVLEGRAVDLVISDMAPNLSGVASADAARIEHLCDLALEFA
QNHLKPDGALLVKCFHGSGYSQIVEKFKQQFKVVAPRKPKASRDKSSETFILGRQLKQPR
>Mature_219_residues
AKNRFNQNWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPGSWSQYARNKLAQGKKRDTQRE
GGIDGTIIALDLLPMEPIADVHFIQGDFREDDVLRQLEDVLEGRAVDLVISDMAPNLSGVASADAARIEHLCDLALEFAQ
NHLKPDGALLVKCFHGSGYSQIVEKFKQQFKVVAPRKPKASRDKSSETFILGRQLKQPR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=218, Percent_Identity=35.7798165137615, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI29029591, Length=203, Percent_Identity=32.0197044334975, Blast_Score=107, Evalue=7e-24,
Organism=Homo sapiens, GI29029589, Length=203, Percent_Identity=32.0197044334975, Blast_Score=107, Evalue=7e-24,
Organism=Homo sapiens, GI7110661, Length=203, Percent_Identity=32.0197044334975, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI194097365, Length=203, Percent_Identity=29.064039408867, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1789569, Length=206, Percent_Identity=42.7184466019417, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17554650, Length=203, Percent_Identity=33.4975369458128, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17553474, Length=226, Percent_Identity=30.9734513274336, Blast_Score=106, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71987550, Length=203, Percent_Identity=30.5418719211823, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71987561, Length=203, Percent_Identity=30.5418719211823, Blast_Score=104, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=203, Percent_Identity=30.5418719211823, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=205, Percent_Identity=31.7073170731707, Blast_Score=111, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6319535, Length=205, Percent_Identity=30.7317073170732, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6321302, Length=122, Percent_Identity=36.0655737704918, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356387, Length=218, Percent_Identity=33.9449541284404, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24647580, Length=204, Percent_Identity=32.843137254902, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648639, Length=203, Percent_Identity=31.5270935960591, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI18859957, Length=196, Percent_Identity=29.5918367346939, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24804; Mature: 24672

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNRFNQNWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPG
CCCCCCCCCHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLAQGKKRDTQREGGIDGTIIALDLLPMEPIADVHFIQGDFREDDVLRQLED
CHHHHHHHHHHCCCHHCHHHHCCCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHH
VLEGRAVDLVISDMAPNLSGVASADAARIEHLCDLALEFAQNHLKPDGALLVKCFHGSGY
HHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQIVEKFKQQFKVVAPRKPKASRDKSSETFILGRQLKQPR
HHHHHHHHHHHEECCCCCCCCCCCCCCCEEEECHHCCCCC
>Mature Secondary Structure 
AKNRFNQNWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPG
CCCCCCCCHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLAQGKKRDTQREGGIDGTIIALDLLPMEPIADVHFIQGDFREDDVLRQLED
CHHHHHHHHHHCCCHHCHHHHCCCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHH
VLEGRAVDLVISDMAPNLSGVASADAARIEHLCDLALEFAQNHLKPDGALLVKCFHGSGY
HHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQIVEKFKQQFKVVAPRKPKASRDKSSETFILGRQLKQPR
HHHHHHHHHHHEECCCCCCCCCCCCCCCEEEECHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA