Click here to switch to the map view.

The map label for this gene is spoT [H]

Identifier: 115350915

GI number: 115350915

Start: 944925

End: 947291

Strand: Direct

Name: spoT [H]

Synonym: Bamb_0861

Alternate gene names: 115350915

Gene position: 944925-947291 (Clockwise)

Preceding gene: 115350914

Following gene: 115350916

Centisome position: 26.57

GC content: 65.15

Gene sequence:

>2367_bases
ATGAGCACCACCCCATCGTCCGCCTCCGCGGATTCGACCACCGAAGCCACGGCCCGGTCGCCTGCGCGCCAGTACATCGA
CGCGGTCCTCGAACAGTCCTTCCGGCATCTGTTCGGGCCGACCGCCACGCCGGAGCAGCCGCGCAAGCACGGCGTCGTTT
CGATCGCGAAACTGACGGCCGCGCTTGCCGAGTATCTCCCTCCGGACGAAATCAAAGAGGTCAAGGCGGCGTTCCACTTC
AGCGACGAAGCCCACCTCGGTCAATATCGCCAGAGCGGCGAACCCTACATCACCCATCCTGTCGCCGTCGCGGAAATCTG
CGCCGGCTGGAAGCTCGACGCGCAAGCCGTGATGGCCGCGCTGCTGCACGACGTGATGGAAGACCAGGGCGTGACCAAGA
GCGAGCTGGCCGAACGTTTCGGCCCGAAGGTCGCCGAACTGGTCGACGGCCTGTCGAAGCTCGACAAGATGGAGTTCCGC
AGCCGCGAGGAAGCGCAGGCGGAAAACTTCCGCAAGATGCTGCTCGCGATGGCACGCGACGTGCGCGTCATCCTCGTCAA
GCTCGCCGACCGTCTGCACAACATGCGCACGCTCGGCGCGGTGCCGATGGAAAAGCGCCGCCGCGTCGCCCGCGAGACGC
TCGACATCTATGCGCCGATCGCGCACCGCCTCGGGTTGAACAACACGTATCGCGAGCTGCAGGACATGAGCTTCGCGAAT
TTCAACCCGCATCGCTACGCGACGCTCGAGAAGGCCGTGAAGGCCGCGCGCGGCAACCGCCGCGAAGTGATCAGCAAGAT
CCTCGAGTCCGCGCAGCGCGCGATGACCGACGCGAAGATCGACGCCGAGATCACCGGCCGCGAAAAGACGATCTACAGCA
TCTACCGCAAGATGCGCGACAAGCAGCTGTCGTTCTCGCAGGTGCTCGACGTGTACGGCTTTCGCGTCGTCGTCGATAGC
CCGCTCGACTGCTACACGTGCATCGGCGCGCTGCACGCGCTGTACAAGCCCGTGCCCGGCAAGTTCAAGGACTACATCGC
GATCCCGAAGATCAACGGCTATCAGTCGCTGCACACGACGCTCGTCGGCCCGTTCGGCGCGCCGATCGAGTTCCAGGTGC
GCACGCGCAAGATGCACGAGATCGCCGAGGCTGGCGTGGCCGCGCACTGGCTGTACAAGAACGGTGGCGCCGATCTGAAC
GACGTGCAGAAGCGCGCGCACCAGTGGCTGAAGTCGCTGCTCGACATTCAGAGCGAAGCCGGTGATTCGAGCGAATTCCT
CGAGCACGTGAAGATCGACCTGTTCCCGGATGCGGTCTACGTGTTCACGCCGAAGTCGAAGATCATGGCGCTGCCGCGCG
GCGCGACGGCGCTCGATTTCGCGTATTCGATCCACAGCGATCTCGGCAACCAGTGCGTGGCCGTGAAGATCAACAACGAG
CTGCTGCCGCTGCGTACCGAGCTGAAGAGCGGCGACATCGTCGAGGTGATCACCGCGCCGTATTCGAAGCCGAACCCCGC
GTGGCTCGGTTTCGTGCGCACCGGCAAGGCGCGCTCGGCGATTCGTCACTATCTGAAGACGATGCGCCTGAACGAGTCGG
TGCAGCTCGGCGAGCGGCTCGTCGATCAAAGCCTGAAGGGTTACGGGCTTGCGCTGGCCGACGTGGCGCCGGAAGTGTGG
GAGAAGCTCGTGCAGTGGACGGGCAACAAGAGCCGCCAGGAAATCTTCGCGGACATCGGCCTCGGCCGCCGCGTCGCCGC
GGTGATGGCCAAGCGCATCGAGGTGCTGACGAGCGGCCGCGATGCGGACGACGATCTGCCGAAGTCCGAGCGGCATCCCG
CGCACAATGCGCCGCCGGTCGTCATTACCGGCACCGAAGGGATGTCCGTGCAGCTGTCGGCATGCTGCCGGCCGATCCCG
GGCGATGCGATCATGGGCTATATCGGCATCGGCCTCGGGATGGCGATTCATACGACCGATTGCCGTGTCGCGCAGCGCAT
CCATCGCCGCGATCCGGGCCGCTGGATCGACGTCGAGTGGGCACCGCAGCCGGGCCGCCTGTTCGACGTGGCAGTGAAGG
CGCTCGTGAAGAACACGAAGGGCATCTTCGCGCGCGTGGCGGCGGACATCACGTCGGCCGACGCGAACATCGTCCATATC
GCCATGGACGAGGATCTGACGCACGAATCGACGGTGCTGCGCTTCGTGATCCAGGTCAGCGATCGCGTGCACCTGGCGAA
CGTGATGCGGCGCGTGCGGACCAATCCGGACGTGATGCGGATCATGCGCGAGCGCTCGACCGACGACAGCGTGCATGCGC
GTCACGACGGCGGCATGCGCATCGAGCGCGAACGGCAGGATTACTGA

Upstream 100 bases:

>100_bases
CGTCGGCGTCGAGATGTTGAAGAAGGTGCCGGTGTAACGCGCATCCGGCCCGTTCCAGCCATGTAATCCACGCGCGCAAC
GACTCGACCTGGAGGCGAAT

Downstream 100 bases:

>100_bases
CGCGACGCGTTGCCTGAGCAGGATATGACGGCGGCCACGGGCCGCCGTTTTCATTGGTGACGCGGGCGAGGTGGCGTGGC
GTGGTGCGGTGGTGGCCCGG

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 788; Mature: 787

Protein sequence:

>788_residues
MSTTPSSASADSTTEATARSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALAEYLPPDEIKEVKAAFHF
SDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFR
SREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
FNPHRYATLEKAVKAARGNRREVISKILESAQRAMTDAKIDAEITGREKTIYSIYRKMRDKQLSFSQVLDVYGFRVVVDS
PLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLN
DVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVAPEVW
EKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLTSGRDADDDLPKSERHPAHNAPPVVITGTEGMSVQLSACCRPIP
GDAIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDSVHARHDGGMRIERERQDY

Sequences:

>Translated_788_residues
MSTTPSSASADSTTEATARSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALAEYLPPDEIKEVKAAFHF
SDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFR
SREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
FNPHRYATLEKAVKAARGNRREVISKILESAQRAMTDAKIDAEITGREKTIYSIYRKMRDKQLSFSQVLDVYGFRVVVDS
PLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLN
DVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVAPEVW
EKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLTSGRDADDDLPKSERHPAHNAPPVVITGTEGMSVQLSACCRPIP
GDAIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDSVHARHDGGMRIERERQDY
>Mature_787_residues
STTPSSASADSTTEATARSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALAEYLPPDEIKEVKAAFHFS
DEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFRS
REEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFANF
NPHRYATLEKAVKAARGNRREVISKILESAQRAMTDAKIDAEITGREKTIYSIYRKMRDKQLSFSQVLDVYGFRVVVDSP
LDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLND
VQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNEL
LPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVAPEVWE
KLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLTSGRDADDDLPKSERHPAHNAPPVVITGTEGMSVQLSACCRPIPG
DAIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHIA
MDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDSVHARHDGGMRIERERQDY

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=710, Percent_Identity=45.9154929577465, Blast_Score=668, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=683, Percent_Identity=31.6251830161054, Blast_Score=385, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 88015; Mature: 87884

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPSSASADSTTEATARSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTA
CCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHH
ALAEYLPPDEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAA
HHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHHH
LLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFRSREEAQAENFRKMLLAMARD
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
FNPHRYATLEKAVKAARGNRREVISKILESAQRAMTDAKIDAEITGREKTIYSIYRKMRD
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHH
KQLSFSQVLDVYGFRVVVDSPLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTT
HCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHH
LVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLNDVQKRAHQWLKSLLDIQSEA
HCCCCCCCEEEEHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
CCHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCC
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERL
EECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHH
VDQSLKGYGLALADVAPEVWEKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLTSGR
HHHHHHCCCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DADDDLPKSERHPAHNAPPVVITGTEGMSVQLSACCRPIPGDAIMGYIGIGLGMAIHTTD
CCCCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHCCCCCCCHHHHHHHHHHHCCEEECCH
CRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDSVHARHDGGMR
EECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEECCCCCCE
IERERQDY
ECHHCCCC
>Mature Secondary Structure 
STTPSSASADSTTEATARSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTA
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHH
ALAEYLPPDEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAA
HHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHHH
LLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFRSREEAQAENFRKMLLAMARD
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
FNPHRYATLEKAVKAARGNRREVISKILESAQRAMTDAKIDAEITGREKTIYSIYRKMRD
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHH
KQLSFSQVLDVYGFRVVVDSPLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTT
HCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHH
LVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLNDVQKRAHQWLKSLLDIQSEA
HCCCCCCCEEEEHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
CCHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCC
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERL
EECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHH
VDQSLKGYGLALADVAPEVWEKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLTSGR
HHHHHHCCCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DADDDLPKSERHPAHNAPPVVITGTEGMSVQLSACCRPIPGDAIMGYIGIGLGMAIHTTD
CCCCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHCCCCCCCHHHHHHHHHHHCCEEECCH
CRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDSVHARHDGGMR
EECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEECCCCCCE
IERERQDY
ECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]