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The map label for this gene is rfbD [H]

Identifier: 115350808

GI number: 115350808

Start: 835579

End: 836475

Strand: Direct

Name: rfbD [H]

Synonym: Bamb_0754

Alternate gene names: 115350808

Gene position: 835579-836475 (Clockwise)

Preceding gene: 115350807

Following gene: 115350809

Centisome position: 23.49

GC content: 63.66

Gene sequence:

>897_bases
TTGAAACAGGACACAAGGCACACGCCCACGATTCTCGTGACCGGGGTAACCGGTCAGATCGGATTCGAATTGTTGCGGGC
GCTCCAAGGGCTCGGCCGAGTGGTGCCGTGCGACCGTTCGGTGCTCGATCTCGCCGATCTCGATCGGGTGCGTGCTTTCG
CGAGAGATCTGAAGCCGGCACTGATCGTGAATCCGGCCGCTTATACGGCGGTGGACACGGCGGAGAGCGAAGTCGAGCTC
GCGCGGCGGCTGAATGTCGATGTGCCGCGCGTGTTCGCTGAAGAAGCGGCGCGTAGCGGTGGCACGCTGATCCATTACTC
GACCGATTATGTGTTCGACGGCACGAAAGTGGGCGCGTATGTGGAAACCGACGCGCCGAATCCCTTGAATGCATATGGTG
CGACGAAGCTGGAGGGTGAGCAGGCGATCGCCGCGACCGGATGTGCGCACGTGATCCTGCGCACGAGCTGGGTATATGGC
CGGCGCGGCAGAAACTTCCTGCGGACGATGCTGAAGCTCGGGGCCGAGCGCCCCGAATTGAGAATCGTCGCGGATCAGGT
CGGCGCGCCGACCTGGGCCGCAACCATCGCCGCCGCGACGTCGCACATCATCGCCCAGGCCGCGGCGTCGGCCGACACCG
ACTGGTGGGCGCGCCGGGCCGGCGTCTATCACCTTACATCTGCCGGAGCGACTTCCTGGTGTGGTTTTGCCGAGGCGATA
TTCGGTATCGCCATGGCTTCTCATGCGCCGAGGGTTGTACCGATTTCCTCGGACGATTACCCGACGCCCGCGAAGCGGCC
GGCCAACTCGAAAATGGCGCTGGGGAAGCTGGCGGAAGTCTTCGGCATGCAGATGCCGGATTGGCGCGATGCATTGCAGT
TGTGCCTGAGCGAATGA

Upstream 100 bases:

>100_bases
ACGATCCGGCAATCGGAATCGAATGGCCGGTCGATGTGGACCCGTTGCTGGCGGCCAAGGACGCCGCGGGGGCGCGACTC
AGCGAAGCCGAGGTCTACGC

Downstream 100 bases:

>100_bases
GCGGTCATGGTGACGTCCGCCGGCGGCCCGCATGAGGGCCAGGCCCGTTCCCGGCGGTTGTCCGGCGCGCCCGGCAGTTT
CCGGGGAAAGGAGTTCGCCG

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKQDTRHTPTILVTGVTGQIGFELLRALQGLGRVVPCDRSVLDLADLDRVRAFARDLKPALIVNPAAYTAVDTAESEVEL
ARRLNVDVPRVFAEEAARSGGTLIHYSTDYVFDGTKVGAYVETDAPNPLNAYGATKLEGEQAIAATGCAHVILRTSWVYG
RRGRNFLRTMLKLGAERPELRIVADQVGAPTWAATIAAATSHIIAQAAASADTDWWARRAGVYHLTSAGATSWCGFAEAI
FGIAMASHAPRVVPISSDDYPTPAKRPANSKMALGKLAEVFGMQMPDWRDALQLCLSE

Sequences:

>Translated_298_residues
MKQDTRHTPTILVTGVTGQIGFELLRALQGLGRVVPCDRSVLDLADLDRVRAFARDLKPALIVNPAAYTAVDTAESEVEL
ARRLNVDVPRVFAEEAARSGGTLIHYSTDYVFDGTKVGAYVETDAPNPLNAYGATKLEGEQAIAATGCAHVILRTSWVYG
RRGRNFLRTMLKLGAERPELRIVADQVGAPTWAATIAAATSHIIAQAAASADTDWWARRAGVYHLTSAGATSWCGFAEAI
FGIAMASHAPRVVPISSDDYPTPAKRPANSKMALGKLAEVFGMQMPDWRDALQLCLSE
>Mature_298_residues
MKQDTRHTPTILVTGVTGQIGFELLRALQGLGRVVPCDRSVLDLADLDRVRAFARDLKPALIVNPAAYTAVDTAESEVEL
ARRLNVDVPRVFAEEAARSGGTLIHYSTDYVFDGTKVGAYVETDAPNPLNAYGATKLEGEQAIAATGCAHVILRTSWVYG
RRGRNFLRTMLKLGAERPELRIVADQVGAPTWAATIAAATSHIIAQAAASADTDWWARRAGVYHLTSAGATSWCGFAEAI
FGIAMASHAPRVVPISSDDYPTPAKRPANSKMALGKLAEVFGMQMPDWRDALQLCLSE

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=171, Percent_Identity=38.0116959064327, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI11034825, Length=159, Percent_Identity=38.9937106918239, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788352, Length=299, Percent_Identity=42.1404682274247, Blast_Score=225, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32108; Mature: 32108

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQDTRHTPTILVTGVTGQIGFELLRALQGLGRVVPCDRSVLDLADLDRVRAFARDLKPA
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCE
LIVNPAAYTAVDTAESEVELARRLNVDVPRVFAEEAARSGGTLIHYSTDYVFDGTKVGAY
EEECCCHHHEHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCEEECCCEECEE
VETDAPNPLNAYGATKLEGEQAIAATGCAHVILRTSWVYGRRGRNFLRTMLKLGAERPEL
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCE
RIVADQVGAPTWAATIAAATSHIIAQAAASADTDWWARRAGVYHLTSAGATSWCGFAEAI
EEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCCHHHHHHHHH
FGIAMASHAPRVVPISSDDYPTPAKRPANSKMALGKLAEVFGMQMPDWRDALQLCLSE
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKQDTRHTPTILVTGVTGQIGFELLRALQGLGRVVPCDRSVLDLADLDRVRAFARDLKPA
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCE
LIVNPAAYTAVDTAESEVELARRLNVDVPRVFAEEAARSGGTLIHYSTDYVFDGTKVGAY
EEECCCHHHEHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCEEECCCEECEE
VETDAPNPLNAYGATKLEGEQAIAATGCAHVILRTSWVYGRRGRNFLRTMLKLGAERPEL
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCE
RIVADQVGAPTWAATIAAATSHIIAQAAASADTDWWARRAGVYHLTSAGATSWCGFAEAI
EEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCCHHHHHHHHH
FGIAMASHAPRVVPISSDDYPTPAKRPANSKMALGKLAEVFGMQMPDWRDALQLCLSE
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]