BacMap

Definition	Burkholderia ambifaria AMMD chromosome chromosome 1, complete sequence.
Accession	NC_008390
Length	3,556,545

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The map label for this gene is cpnA [H]

Identifier: 115350579

GI number: 115350579

Start: 590533

End: 591348

Strand: Direct

Name: cpnA [H]

Synonym: Bamb_0525

Alternate gene names: 115350579

Gene position: 590533-591348 (Clockwise)

Preceding gene: 115350578

Following gene: 115350580

Centisome position: 16.6

GC content: 70.71

Gene sequence:

>816_bases
ATGACCATCGAAACTTCCGCGCAGGATCCGCGCGCCGCGAGCGACGCGCGCTATGCGCGCTACCCGAGCCTCGCGGATCG
CGCGGTGCTGATCACGGGCGGTGCGACCGGGATCGGCGCGGCGTTCGTCGAGCACTTCGCGCGGCAGGGCGCGCGCGTCG
CGTTCGTCGATCTCGACGCGCAGGCGGGCCAGGCGCTCGCGGAGAGCCTCGCGCAGATGCCGGAGGTACGCCACGCGCCG
CTGTTCCTGCCGTGCGACCTGACCGACATCGACGCGCTGCGCCATGCGATCGACGCGATCCGCGCGCGGATCGGCGCGAT
CGCGGTGCTCGTGAACAACGCGGCGAACGACACGCGCCACGCGATCGCCGACGTGACACCCGCGTCGTTCGACGCGGGCA
TCGCGGTGAACCTGCGCCACCAGTTCTTCGCCGCGCAGGCGGTGATCGACGACATGAAGCAGCGCGGCGGCGGCGCGATC
ATCAATCTCGGCTCGATCAGCTGGATGCTGAAGAACGGCGGCTATCCCGTCTACGTGATGGCGAAGGCGGCCGTGCAGGG
GCTCACGCGCGGGCTCGCGCGCGATCTCGGCCCGTTCGGGATCCGCGTGAATTCGCTGGTGCCCGGCTGGGTGATGACCG
ACAAGCAGCGCAAGCTCTGGCTCGACGACGCGGGCCGCGCGGCGATCAAGGCGGGCCAGTGCATCGACGCCGAGCTGCTG
CCTGATGATCTCGCGCGCATGGCGCTGTTTCTCGCGGCCGACGACAGCCGGATGATCACCGCGCAGGACGTGGTGGTCGA
CGGCGGCTGGGCCTGA

Upstream 100 bases:

>100_bases
AGTACCTGGTGCGCGGCGCGATCCTGCTCGCGGCCGTGCTGCTCGACCAGCTGAAGAACCGCGGCGTACGCGACTGACCG
ATTCCACGGAGACGATTCGC

Downstream 100 bases:

>100_bases
TTGTAGGTATGGGTGCTGGGGTTCGCTCGGCACCGTGCGACGACCCGTATCGTTTCATCGACGAAGGAAAGGAGTTCACC
ATGACCGCCACGTCCTCGCG

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTIETSAQDPRAASDARYARYPSLADRAVLITGGATGIGAAFVEHFARQGARVAFVDLDAQAGQALAESLAQMPEVRHAP
LFLPCDLTDIDALRHAIDAIRARIGAIAVLVNNAANDTRHAIADVTPASFDAGIAVNLRHQFFAAQAVIDDMKQRGGGAI
INLGSISWMLKNGGYPVYVMAKAAVQGLTRGLARDLGPFGIRVNSLVPGWVMTDKQRKLWLDDAGRAAIKAGQCIDAELL
PDDLARMALFLAADDSRMITAQDVVVDGGWA

Sequences:

>Translated_271_residues
MTIETSAQDPRAASDARYARYPSLADRAVLITGGATGIGAAFVEHFARQGARVAFVDLDAQAGQALAESLAQMPEVRHAP
LFLPCDLTDIDALRHAIDAIRARIGAIAVLVNNAANDTRHAIADVTPASFDAGIAVNLRHQFFAAQAVIDDMKQRGGGAI
INLGSISWMLKNGGYPVYVMAKAAVQGLTRGLARDLGPFGIRVNSLVPGWVMTDKQRKLWLDDAGRAAIKAGQCIDAELL
PDDLARMALFLAADDSRMITAQDVVVDGGWA
>Mature_270_residues
TIETSAQDPRAASDARYARYPSLADRAVLITGGATGIGAAFVEHFARQGARVAFVDLDAQAGQALAESLAQMPEVRHAPL
FLPCDLTDIDALRHAIDAIRARIGAIAVLVNNAANDTRHAIADVTPASFDAGIAVNLRHQFFAAQAVIDDMKQRGGGAII
NLGSISWMLKNGGYPVYVMAKAAVQGLTRGLARDLGPFGIRVNSLVPGWVMTDKQRKLWLDDAGRAAIKAGQCIDAELLP
DDLARMALFLAADDSRMITAQDVVVDGGWA

Specific function: Reduces cyclopentanol to cyclopentanone. The enzyme also shows considerable activity toward cyclohexanol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=265, Percent_Identity=33.5849056603774, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI15277342, Length=249, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI7705925, Length=256, Percent_Identity=34.765625, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI31542939, Length=192, Percent_Identity=32.8125, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI19923817, Length=254, Percent_Identity=25.9842519685039, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=27.3469387755102, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI126723750, Length=268, Percent_Identity=27.2388059701493, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI66933014, Length=235, Percent_Identity=28.0851063829787, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI59889578, Length=236, Percent_Identity=27.9661016949153, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4758504, Length=201, Percent_Identity=30.8457711442786, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI224922801, Length=170, Percent_Identity=31.7647058823529, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI10190704, Length=250, Percent_Identity=28.8, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI126723191, Length=218, Percent_Identity=27.5229357798165, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI5031737, Length=250, Percent_Identity=24.8, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4503817, Length=185, Percent_Identity=25.4054054054054, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI302344755, Length=126, Percent_Identity=34.1269841269841, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI83715985, Length=189, Percent_Identity=29.6296296296296, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790717, Length=247, Percent_Identity=32.3886639676113, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787335, Length=243, Percent_Identity=31.6872427983539, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082100, Length=253, Percent_Identity=30.0395256916996, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789208, Length=249, Percent_Identity=28.5140562248996, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=29.3650793650794, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082160, Length=257, Percent_Identity=28.7937743190661, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI2367365, Length=249, Percent_Identity=30.1204819277108, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789378, Length=259, Percent_Identity=30.1158301158301, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786812, Length=254, Percent_Identity=29.1338582677165, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI2367175, Length=190, Percent_Identity=29.4736842105263, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789057, Length=256, Percent_Identity=30.46875, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=250, Percent_Identity=32, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25147288, Length=253, Percent_Identity=30.0395256916996, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI72000259, Length=269, Percent_Identity=29.7397769516729, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560332, Length=274, Percent_Identity=30.2919708029197, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17563726, Length=265, Percent_Identity=28.6792452830189, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17555706, Length=249, Percent_Identity=28.1124497991968, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17561402, Length=249, Percent_Identity=29.718875502008, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17559104, Length=267, Percent_Identity=28.0898876404494, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562904, Length=266, Percent_Identity=30.0751879699248, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17560150, Length=265, Percent_Identity=28.3018867924528, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71994600, Length=249, Percent_Identity=26.1044176706827, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=272, Percent_Identity=28.3088235294118, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71994604, Length=244, Percent_Identity=25.4098360655738, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=273, Percent_Identity=26.007326007326, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17567345, Length=193, Percent_Identity=26.4248704663212, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17551412, Length=271, Percent_Identity=28.4132841328413, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562908, Length=259, Percent_Identity=26.2548262548263, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=251, Percent_Identity=24.3027888446215, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17562990, Length=275, Percent_Identity=28, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=270, Percent_Identity=28.1481481481481, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=259, Percent_Identity=25.8687258687259, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=267, Percent_Identity=26.9662921348315, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=274, Percent_Identity=27.007299270073, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17536025, Length=255, Percent_Identity=25.8823529411765, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=198, Percent_Identity=28.7878787878788, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17565030, Length=268, Percent_Identity=27.6119402985075, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508651, Length=248, Percent_Identity=24.5967741935484, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=249, Percent_Identity=24.8995983935743, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322861, Length=190, Percent_Identity=27.8947368421053, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357041, Length=255, Percent_Identity=30.1960784313725, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24643142, Length=248, Percent_Identity=30.241935483871, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI23397609, Length=201, Percent_Identity=31.8407960199005, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI28571526, Length=252, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24639444, Length=259, Percent_Identity=28.957528957529, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=24.1935483870968, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281364892, Length=186, Percent_Identity=31.1827956989247, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24644337, Length=251, Percent_Identity=29.8804780876494, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24644339, Length=253, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17737361, Length=205, Percent_Identity=29.2682926829268, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24641232, Length=236, Percent_Identity=28.3898305084746, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21358495, Length=230, Percent_Identity=24.3478260869565, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.163 [H]

Molecular weight: Translated: 28773; Mature: 28642

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIETSAQDPRAASDARYARYPSLADRAVLITGGATGIGAAFVEHFARQGARVAFVDLDA
CCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCC
QAGQALAESLAQMPEVRHAPLFLPCDLTDIDALRHAIDAIRARIGAIAVLVNNAANDTRH
HHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHH
AIADVTPASFDAGIAVNLRHQFFAAQAVIDDMKQRGGGAIINLGSISWMLKNGGYPVYVM
HHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCEEEEE
AKAAVQGLTRGLARDLGPFGIRVNSLVPGWVMTDKQRKLWLDDAGRAAIKAGQCIDAELL
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEECCCCEEEECCCCCHHHHCCCCCCCCCC
PDDLARMALFLAADDSRMITAQDVVVDGGWA
HHHHHHHEEHEECCCCCEEEEEEEEECCCCC
>Mature Secondary Structure 
TIETSAQDPRAASDARYARYPSLADRAVLITGGATGIGAAFVEHFARQGARVAFVDLDA
CCCCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCC
QAGQALAESLAQMPEVRHAPLFLPCDLTDIDALRHAIDAIRARIGAIAVLVNNAANDTRH
HHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHH
AIADVTPASFDAGIAVNLRHQFFAAQAVIDDMKQRGGGAIINLGSISWMLKNGGYPVYVM
HHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCEEEEE
AKAAVQGLTRGLARDLGPFGIRVNSLVPGWVMTDKQRKLWLDDAGRAAIKAGQCIDAELL
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEECCCCEEEECCCCCHHHHCCCCCCCCCC
PDDLARMALFLAADDSRMITAQDVVVDGGWA
HHHHHHHEEHEECCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12406764 [H]