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The map label for this gene is araF [H]

Identifier: 115350576

GI number: 115350576

Start: 586827

End: 587822

Strand: Direct

Name: araF [H]

Synonym: Bamb_0522

Alternate gene names: 115350576

Gene position: 586827-587822 (Clockwise)

Preceding gene: 115350575

Following gene: 115350577

Centisome position: 16.5

GC content: 65.76

Gene sequence:

>996_bases
ATGAAACGCAGAACGTTCGTAACGCTGGCCGCCGCGGCGGCGGTGGTGATGGGCAGTCCGGTCGCGCACGCGGCCGATCC
GGTCAAGATCGGCTTTCTGGTGAAGCAGCCGGAAGAGCCGTGGTTCCAGGACGAATGGAAATTCGCCGAGATGGCCGCGA
AGGACAAGGGCTTCACGCTCGTGAAGATCGGCGCGCCGTCGGGCGAGAAGGTGATGAGCGCGATCGACAACCTGTCCGCG
CAGAAGGTGCAGGGCTTCATCATCTGCACGCCCGACGTGAAGCTCGGGCCGGGCATCGTCGCGAAGGCGAAGTCGCACAA
CCTGAAGATGATGACGGTTGACGACCGCCTCGTCGACGGCGCGGGCAAGCCGATCGAGTCGGTGCCGCACATGGGGATTT
CCGCGTACAACATCGGCAAGCAGGTCGGCGACGGCATCGCGGCCGAGATCAAGAAGCGCGGCTGGAACATGAAGGAAGTC
GGCGCGATCGACATCACGTACGAGCAGCTGCCGACCGCGCACGACCGCACGAGCGGCGCGACCGACGCGCTGGTGGCGGC
CGGCTTCCCGAAGGCGAACGTGATCGCGGCGCCGCAGGCGAAGACCGACACCGAGAACGCGTTCAACGCGGCGAACATCG
CGCTCACCAAGAACCCGCAGTTCAAGCACTGGGTCGCCTACGGCCTGAACGACGAAGCCGTGCTCGGCGCGGTGCGCGCG
GCCGAAGGGCGCGGCTTCAAGGCGGACAACATGATCGGCATCGGTATCGGCGGTTCCGACTCGGCGCTCAGCGAATTCAA
GAAGCCGCAGCCGACGGGCTTCTACGGCACCGTGATCATCAGCCCGAAGCGCCACGGCGAGGAAACCTCGGACCTGATGT
ACGCATGGATCACGCAGGGCAAGGCACCGCCGGCGCTGACGCTGACGACGGGCATGCTCGCGACGCGCGACAACGTGTCG
AAGGTGCGCGACGAGATGGGGCTCGCATCGAAGTAA

Upstream 100 bases:

>100_bases
CAACGATTCGAAAGACCGCACGTGCGACGGCCGCGCGCACGCTGCGTCGAAACCAACAAGCGGCTGCATCCCGTTCGATG
AAGACAAGGAGACACTGGAG

Downstream 100 bases:

>100_bases
GCGGCTTGCCGGCTGCCGCGGCGATGCGGCAGCCGGCGATCGATGGATCTGCAAAGTGGGGAGACGACGTGTCAGCGGCA
CTGCGTTTTGACAATATCGG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; arabinose [Cytoplasm] [C]

Alternate protein names: ABP [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKRRTFVTLAAAAAVVMGSPVAHAADPVKIGFLVKQPEEPWFQDEWKFAEMAAKDKGFTLVKIGAPSGEKVMSAIDNLSA
QKVQGFIICTPDVKLGPGIVAKAKSHNLKMMTVDDRLVDGAGKPIESVPHMGISAYNIGKQVGDGIAAEIKKRGWNMKEV
GAIDITYEQLPTAHDRTSGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPQFKHWVAYGLNDEAVLGAVRA
AEGRGFKADNMIGIGIGGSDSALSEFKKPQPTGFYGTVIISPKRHGEETSDLMYAWITQGKAPPALTLTTGMLATRDNVS
KVRDEMGLASK

Sequences:

>Translated_331_residues
MKRRTFVTLAAAAAVVMGSPVAHAADPVKIGFLVKQPEEPWFQDEWKFAEMAAKDKGFTLVKIGAPSGEKVMSAIDNLSA
QKVQGFIICTPDVKLGPGIVAKAKSHNLKMMTVDDRLVDGAGKPIESVPHMGISAYNIGKQVGDGIAAEIKKRGWNMKEV
GAIDITYEQLPTAHDRTSGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPQFKHWVAYGLNDEAVLGAVRA
AEGRGFKADNMIGIGIGGSDSALSEFKKPQPTGFYGTVIISPKRHGEETSDLMYAWITQGKAPPALTLTTGMLATRDNVS
KVRDEMGLASK
>Mature_331_residues
MKRRTFVTLAAAAAVVMGSPVAHAADPVKIGFLVKQPEEPWFQDEWKFAEMAAKDKGFTLVKIGAPSGEKVMSAIDNLSA
QKVQGFIICTPDVKLGPGIVAKAKSHNLKMMTVDDRLVDGAGKPIESVPHMGISAYNIGKQVGDGIAAEIKKRGWNMKEV
GAIDITYEQLPTAHDRTSGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPQFKHWVAYGLNDEAVLGAVRA
AEGRGFKADNMIGIGIGGSDSALSEFKKPQPTGFYGTVIISPKRHGEETSDLMYAWITQGKAPPALTLTTGMLATRDNVS
KVRDEMGLASK

Specific function: Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D- fucose (approximately 40-fold reduction) [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788211, Length=332, Percent_Identity=47.289156626506, Blast_Score=315, Evalue=3e-87,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35178; Mature: 35178

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRTFVTLAAAAAVVMGSPVAHAADPVKIGFLVKQPEEPWFQDEWKFAEMAAKDKGFTL
CCCCEEEHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCEEE
VKIGAPSGEKVMSAIDNLSAQKVQGFIICTPDVKLGPGIVAKAKSHNLKMMTVDDRLVDG
EEECCCCCHHHHHHHHCCCHHHHCEEEEECCCCCCCCCEEEECCCCCEEEEEECCCEECC
AGKPIESVPHMGISAYNIGKQVGDGIAAEIKKRGWNMKEVGAIDITYEQLPTAHDRTSGA
CCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHCCEEEEEHHHCCCCCCCCCCC
TDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPQFKHWVAYGLNDEAVLGAVRA
CHHHEECCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHH
AEGRGFKADNMIGIGIGGSDSALSEFKKPQPTGFYGTVIISPKRHGEETSDLMYAWITQG
CCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHEEEEEEECC
KAPPALTLTTGMLATRDNVSKVRDEMGLASK
CCCCEEEEECCCEECCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKRRTFVTLAAAAAVVMGSPVAHAADPVKIGFLVKQPEEPWFQDEWKFAEMAAKDKGFTL
CCCCEEEHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCEEE
VKIGAPSGEKVMSAIDNLSAQKVQGFIICTPDVKLGPGIVAKAKSHNLKMMTVDDRLVDG
EEECCCCCHHHHHHHHCCCHHHHCEEEEECCCCCCCCCEEEECCCCCEEEEEECCCEECC
AGKPIESVPHMGISAYNIGKQVGDGIAAEIKKRGWNMKEVGAIDITYEQLPTAHDRTSGA
CCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHCCEEEEEHHHCCCCCCCCCCC
TDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPQFKHWVAYGLNDEAVLGAVRA
CHHHEECCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHH
AEGRGFKADNMIGIGIGGSDSALSEFKKPQPTGFYGTVIISPKRHGEETSDLMYAWITQG
CCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHEEEEEEECC
KAPPALTLTTGMLATRDNVSKVRDEMGLASK
CCCCEEEEECCCEECCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arabinose [Periplasm]; H2O [C]

Specific reaction: ATP + arabinose [Periplasm] + H2O = ADP + phosphate + arabinose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2445996; 9097040; 9278503; 6885805; 326784; 9298646; 7031057; 326785; 2204627 [H]