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Identifier: 11499300

GI number: 11499300

Start: 1533144

End: 1534169

Strand: Reverse

Name: Not Available

Synonym: AF1710

Alternate gene names: 11499300

Gene position: 1534169-1533144 (Counterclockwise)

Preceding gene: 11499301

Following gene: 11499299

Centisome position: 70.43

GC content: 39.57

Gene sequence:

>1026_bases
TTGCCTAAAGGCTCGATTATTGTCTCAACCCGTGCGCCAGTCGGTTATGTTGCTATCGTAGATGAGGACACTACATTTAA
CCAAGGCTGCAAAGGTTTAATTCCAAAATCTTCAGAAATTAACACAGAGTTTTATTGCTATTACTTGCTATTGATTAAAC
GGAAACTCGAACAATTAAGTGGTGGAAGCACCTTTAAAGAATTGCCGAAAAAGTCTCTTGAGGAATTACTAATTCCACTA
CTTCCGCTCCCAGAACAGCAAAAAATCGCCGAAATTCTCAGCACGGTTGATAAAGCCATAGAAAAGGTTGACGAAGCCAT
AGCGAAGACTGAAAGGCTGAAGAAGGGCCTGATGCAGGAGCTTCTCACCAAAGGGATAGGGCACAAGGAGTTTAAAGATA
CCGAAATCGGGAGGATTCCGAAGGAGTGGGAGGTTGTTAGGCTTGGGGATGTTTTGGAATTGTGCCAGTATGGGTTATCA
GTTCCGCTGAAGGATAAGGGTAAATATCCAGTTATTAGAATGGATGAAATTGTAAATGGGTATGTTGTTACAGATATTGC
CAAGTATGCAGATCTTGATGAAGAAACGTTCAAGAATTTCAAATTAGAAAAAGGAGATGTTTTATTCAACCGCACAAATA
GTTTAGAACTTGTTGGGAGGACAGGTATATTTTTATTGGATGGTTATTATGTTTTTGCTTCTTATCTGATTAGATTGAGG
CCTAAGCATGAAATTTTGCATCCTCATTTCCTAACGTTCTACCTTATATTTTCACAGAGTAGGCTTAAGCAATTGGCAAC
TGTTGCAGTTCATCAAGCAAATATCAATGCAACAAACTTGAAAAAGTTTAAAATCCCACTCCCACCTCTCCCAGAACAGC
AAAAAATCGCTGAAATTCTTTCAACAGTGGACAAAAAGCTCGAACTCGAAAGGAAACGAAAGGAAAAGCTTGAGAGGATT
AAGAAGGGTTTGATGAACGATTTGCTGACTGGAAGGAGGAGGGTTAGGGTGAAAGCAAATGAATAA

Upstream 100 bases:

>100_bases
AACTCCAACTGATTTAAGTAAACTAAATGGGAGAACACATATTTCCGTTTCAGAAAGAAAAATAACTGAGAAAGCATTAA
GAGATTACAACCTTACATTA

Downstream 100 bases:

>100_bases
AAAAGGAAACACGGAGGAAATTATAGAACGCTTATTTTCCATTTTATCATCCGAAAAGGACTCAATAGAATTTCTTAAAG
CTGTTTCAGAATTTTCAAAA

Product: type I restriction-modification enzyme, S subunit

Products: NA

Alternate protein names: S.MjaXIP; Type I restriction enzyme MjaXIP specificity protein; S protein [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MPKGSIIVSTRAPVGYVAIVDEDTTFNQGCKGLIPKSSEINTEFYCYYLLLIKRKLEQLSGGSTFKELPKKSLEELLIPL
LPLPEQQKIAEILSTVDKAIEKVDEAIAKTERLKKGLMQELLTKGIGHKEFKDTEIGRIPKEWEVVRLGDVLELCQYGLS
VPLKDKGKYPVIRMDEIVNGYVVTDIAKYADLDEETFKNFKLEKGDVLFNRTNSLELVGRTGIFLLDGYYVFASYLIRLR
PKHEILHPHFLTFYLIFSQSRLKQLATVAVHQANINATNLKKFKIPLPPLPEQQKIAEILSTVDKKLELERKRKEKLERI
KKGLMNDLLTGRRRVRVKANE

Sequences:

>Translated_341_residues
MPKGSIIVSTRAPVGYVAIVDEDTTFNQGCKGLIPKSSEINTEFYCYYLLLIKRKLEQLSGGSTFKELPKKSLEELLIPL
LPLPEQQKIAEILSTVDKAIEKVDEAIAKTERLKKGLMQELLTKGIGHKEFKDTEIGRIPKEWEVVRLGDVLELCQYGLS
VPLKDKGKYPVIRMDEIVNGYVVTDIAKYADLDEETFKNFKLEKGDVLFNRTNSLELVGRTGIFLLDGYYVFASYLIRLR
PKHEILHPHFLTFYLIFSQSRLKQLATVAVHQANINATNLKKFKIPLPPLPEQQKIAEILSTVDKKLELERKRKEKLERI
KKGLMNDLLTGRRRVRVKANE
>Mature_340_residues
PKGSIIVSTRAPVGYVAIVDEDTTFNQGCKGLIPKSSEINTEFYCYYLLLIKRKLEQLSGGSTFKELPKKSLEELLIPLL
PLPEQQKIAEILSTVDKAIEKVDEAIAKTERLKKGLMQELLTKGIGHKEFKDTEIGRIPKEWEVVRLGDVLELCQYGLSV
PLKDKGKYPVIRMDEIVNGYVVTDIAKYADLDEETFKNFKLEKGDVLFNRTNSLELVGRTGIFLLDGYYVFASYLIRLRP
KHEILHPHFLTFYLIFSQSRLKQLATVAVHQANINATNLKKFKIPLPPLPEQQKIAEILSTVDKKLELERKRKEKLERIK
KGLMNDLLTGRRRVRVKANE

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=304, Percent_Identity=25, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 39041; Mature: 38910

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKGSIIVSTRAPVGYVAIVDEDTTFNQGCKGLIPKSSEINTEFYCYYLLLIKRKLEQLS
CCCCCEEEEECCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
GGSTFKELPKKSLEELLIPLLPLPEQQKIAEILSTVDKAIEKVDEAIAKTERLKKGLMQE
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTKGIGHKEFKDTEIGRIPKEWEVVRLGDVLELCQYGLSVPLKDKGKYPVIRMDEIVNG
HHHHCCCCCCCCCCHHCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHCC
YVVTDIAKYADLDEETFKNFKLEKGDVLFNRTNSLELVGRTGIFLLDGYYVFASYLIRLR
HHHHHHHHHHCCCHHHHHCCCCCCCCEEEECCCCEEEEECCCEEEEHHHHHHHHHHHHCC
PKHEILHPHFLTFYLIFSQSRLKQLATVAVHQANINATNLKKFKIPLPPLPEQQKIAEIL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHH
STVDKKLELERKRKEKLERIKKGLMNDLLTGRRRVRVKANE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure 
PKGSIIVSTRAPVGYVAIVDEDTTFNQGCKGLIPKSSEINTEFYCYYLLLIKRKLEQLS
CCCCEEEEECCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
GGSTFKELPKKSLEELLIPLLPLPEQQKIAEILSTVDKAIEKVDEAIAKTERLKKGLMQE
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTKGIGHKEFKDTEIGRIPKEWEVVRLGDVLELCQYGLSVPLKDKGKYPVIRMDEIVNG
HHHHCCCCCCCCCCHHCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHCC
YVVTDIAKYADLDEETFKNFKLEKGDVLFNRTNSLELVGRTGIFLLDGYYVFASYLIRLR
HHHHHHHHHHCCCHHHHHCCCCCCCCEEEECCCCEEEEECCCEEEEHHHHHHHHHHHHCC
PKHEILHPHFLTFYLIFSQSRLKQLATVAVHQANINATNLKKFKIPLPPLPEQQKIAEIL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHH
STVDKKLELERKRKEKLERIKKGLMNDLLTGRRRVRVKANE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]