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Identifier: 11499267

GI number: 11499267

Start: 1505856

End: 1507091

Strand: Reverse

Name: Not Available

Synonym: AF1677

Alternate gene names: 11499267

Gene position: 1507091-1505856 (Counterclockwise)

Preceding gene: 11499268

Following gene: 11499265

Centisome position: 69.18

GC content: 47.73

Gene sequence:

>1236_bases
GTGGAGGAAAAGGTAGGCATCGAATGCTACATCACCTCTACTCCCGGAATGGGTGGAGAGATTAAGGCTGAGCCTGAGGA
TTTCTACGTCGAAGAGATTGCAGAGTTTAATTTGAGCGATGAAGGGGATTTTCTGATAATCAGGGTGGAAAAGAAAAACT
GGGATACGCTTAACTTCGCCAGAGTGCTTTCCAACGCTCTCGGCATAAGCCAGAAAAGGATAAGCTTCGCTGGGACGAAG
GACAAAAGAGCGCTGACTGTGCAGTATTTTTCCATTTACGGAGTAAAGAAAGAAGAAATTGAGAGAGTAAACCTGAAAGA
CGCGAAGATAGAGGTCATCGGATATGCAAGGAGAGCAATTCAGCTAGGGGATTTGCTCGGAAACTTTTTCAGAATCAGGG
TTTACGGCTGCAGGGACGGAGAGATATTTCAGGAGACGAGAAACGAGTTAATGGAGAAGGGAACTCCAAATTTTTTCGGT
CTTCAGCGCTTTGGCTCAATAAGGTTCATCACGCATGAGGTGGGGAAGCTCATTCTTCAGAATAACTACGAGGAGGCGTT
TTGGGTTTACGTCGCAAAGCCCTTTGAGGGTGAGAACGAGGAGGTCAGAAAAATAAGGGAAATTCTGTGGGAAACGAGGG
ATGCAAAGCTCGGTTTGAGAGAGCTGCCAAAATACCTGCGATATGAGAGGAATTTGCTTCAAAAGCTCAGAGAGGGAAAA
AGCGAGGAGGAGGCTTTGCTCTCACTGCCGAAAAACCTGAAAATGATGTTCGTTCACGCCTACCAGTCTTACATCTTTAA
CAGGCTCCTTTCAGAGAGGATAAGACAGTTCGGCAGCCTAAAAACTCTGGAAGAGGGGGACTTTGCGTGCTACCTGACCT
TCAAAACACGTCCAACCTTCTCGGACTGCAGCGAGGTTGAAGTTAACGAGGCGAGAGTTAGGTTCCTCGTAAAGGAGAGA
GTCGCTTCTCTGGCCTTGCCGCTTGTTGGTTACGATACGAAGCTGAAGGGGTGGAGCAGAATAGCCCTCGATTTTCTCAG
CGAGGACAATTTAGACCTTAGCAGCTTTAAGACTAAGCACAAGGAGTTCTCATCCAGCGGCTCCTACAGACCTGCGGACA
CTCTCATAGAGCACACAGGTCTGAGTTTTACTGACAGCACATTCAGCTTTTACCTGCCGCGTGGTTGCTACGCAACCGTC
TTCCTGAGAGAGTTTCTCAAGACAGAGCTTTCCTGA

Upstream 100 bases:

>100_bases
GCTTCAGAACTCCATTGAGATCGACACCGATCCCTAATTTTTTATTTTTTCTGCTAATTACTTCAGGCAAAGATATAAAG
CTATTGGGTCATGCTGCAAT

Downstream 100 bases:

>100_bases
CGTGCCTCACCAGCTCATCCATGACCTCCCCCGCCTTCCCTCTCAGGCTGTAATCTGCAATCGGTGTCAAAGGTGTCTCG
TCCGGGTTTATTTCGATGAT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MEEKVGIECYITSTPGMGGEIKAEPEDFYVEEIAEFNLSDEGDFLIIRVEKKNWDTLNFARVLSNALGISQKRISFAGTK
DKRALTVQYFSIYGVKKEEIERVNLKDAKIEVIGYARRAIQLGDLLGNFFRIRVYGCRDGEIFQETRNELMEKGTPNFFG
LQRFGSIRFITHEVGKLILQNNYEEAFWVYVAKPFEGENEEVRKIREILWETRDAKLGLRELPKYLRYERNLLQKLREGK
SEEEALLSLPKNLKMMFVHAYQSYIFNRLLSERIRQFGSLKTLEEGDFACYLTFKTRPTFSDCSEVEVNEARVRFLVKER
VASLALPLVGYDTKLKGWSRIALDFLSEDNLDLSSFKTKHKEFSSSGSYRPADTLIEHTGLSFTDSTFSFYLPRGCYATV
FLREFLKTELS

Sequences:

>Translated_411_residues
MEEKVGIECYITSTPGMGGEIKAEPEDFYVEEIAEFNLSDEGDFLIIRVEKKNWDTLNFARVLSNALGISQKRISFAGTK
DKRALTVQYFSIYGVKKEEIERVNLKDAKIEVIGYARRAIQLGDLLGNFFRIRVYGCRDGEIFQETRNELMEKGTPNFFG
LQRFGSIRFITHEVGKLILQNNYEEAFWVYVAKPFEGENEEVRKIREILWETRDAKLGLRELPKYLRYERNLLQKLREGK
SEEEALLSLPKNLKMMFVHAYQSYIFNRLLSERIRQFGSLKTLEEGDFACYLTFKTRPTFSDCSEVEVNEARVRFLVKER
VASLALPLVGYDTKLKGWSRIALDFLSEDNLDLSSFKTKHKEFSSSGSYRPADTLIEHTGLSFTDSTFSFYLPRGCYATV
FLREFLKTELS
>Mature_411_residues
MEEKVGIECYITSTPGMGGEIKAEPEDFYVEEIAEFNLSDEGDFLIIRVEKKNWDTLNFARVLSNALGISQKRISFAGTK
DKRALTVQYFSIYGVKKEEIERVNLKDAKIEVIGYARRAIQLGDLLGNFFRIRVYGCRDGEIFQETRNELMEKGTPNFFG
LQRFGSIRFITHEVGKLILQNNYEEAFWVYVAKPFEGENEEVRKIREILWETRDAKLGLRELPKYLRYERNLLQKLREGK
SEEEALLSLPKNLKMMFVHAYQSYIFNRLLSERIRQFGSLKTLEEGDFACYLTFKTRPTFSDCSEVEVNEARVRFLVKER
VASLALPLVGYDTKLKGWSRIALDFLSEDNLDLSSFKTKHKEFSSSGSYRPADTLIEHTGLSFTDSTFSFYLPRGCYATV
FLREFLKTELS

Specific function: Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Homo sapiens, GI50727002, Length=406, Percent_Identity=30.2955665024631, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI148762985, Length=411, Percent_Identity=27.007299270073, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI148762961, Length=411, Percent_Identity=27.007299270073, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI148762948, Length=411, Percent_Identity=27.007299270073, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI1789102, Length=158, Percent_Identity=34.1772151898734, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17557232, Length=239, Percent_Identity=28.4518828451883, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6324817, Length=453, Percent_Identity=27.5938189845475, Blast_Score=152, Evalue=9e-38,
Organism=Drosophila melanogaster, GI21355799, Length=451, Percent_Identity=29.490022172949, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_ARCFU (O28596)

Other databases:

- EMBL:   AE000782
- PIR:   D69459
- RefSeq:   NP_070505.1
- ProteinModelPortal:   O28596
- SMR:   O28596
- GeneID:   1484900
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1677
- NMPDR:   fig|224325.1.peg.1666
- TIGR:   AF_1677
- HOGENOM:   HBG541221
- OMA:   AYIGYQR
- ProtClustDB:   CLSK2746992
- BioCyc:   AFUL224325:AF_1677-MONOMER
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 47716; Mature: 47716

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKVGIECYITSTPGMGGEIKAEPEDFYVEEIAEFNLSDEGDFLIIRVEKKNWDTLNFA
CCCCCEEEEEEEECCCCCCEECCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHH
RVLSNALGISQKRISFAGTKDKRALTVQYFSIYGVKKEEIERVNLKDAKIEVIGYARRAI
HHHHHHHCCCHHHEEECCCCCCCEEEEEEEEEECCCHHHHHHCCCCCCEEEEEHHHHHHH
QLGDLLGNFFRIRVYGCRDGEIFQETRNELMEKGTPNFFGLQRFGSIRFITHEVGKLILQ
HHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEHHHHCCCEEEHHHHHHHHHHC
NNYEEAFWVYVAKPFEGENEEVRKIREILWETRDAKLGLRELPKYLRYERNLLQKLREGK
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
SEEEALLSLPKNLKMMFVHAYQSYIFNRLLSERIRQFGSLKTLEEGDFACYLTFKTRPTF
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCC
SDCSEVEVNEARVRFLVKERVASLALPLVGYDTKLKGWSRIALDFLSEDNLDLSSFKTKH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
KEFSSSGSYRPADTLIEHTGLSFTDSTFSFYLPRGCYATVFLREFLKTELS
HHHCCCCCCCCHHHHHHHCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEEKVGIECYITSTPGMGGEIKAEPEDFYVEEIAEFNLSDEGDFLIIRVEKKNWDTLNFA
CCCCCEEEEEEEECCCCCCEECCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHH
RVLSNALGISQKRISFAGTKDKRALTVQYFSIYGVKKEEIERVNLKDAKIEVIGYARRAI
HHHHHHHCCCHHHEEECCCCCCCEEEEEEEEEECCCHHHHHHCCCCCCEEEEEHHHHHHH
QLGDLLGNFFRIRVYGCRDGEIFQETRNELMEKGTPNFFGLQRFGSIRFITHEVGKLILQ
HHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEHHHHCCCEEEHHHHHHHHHHC
NNYEEAFWVYVAKPFEGENEEVRKIREILWETRDAKLGLRELPKYLRYERNLLQKLREGK
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
SEEEALLSLPKNLKMMFVHAYQSYIFNRLLSERIRQFGSLKTLEEGDFACYLTFKTRPTF
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCC
SDCSEVEVNEARVRFLVKERVASLALPLVGYDTKLKGWSRIALDFLSEDNLDLSSFKTKH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
KEFSSSGSYRPADTLIEHTGLSFTDSTFSFYLPRGCYATVFLREFLKTELS
HHHCCCCCCCCHHHHHHHCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475