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The map label for this gene is ispB

Identifier: 11499146

GI number: 11499146

Start: 1396046

End: 1396924

Strand: Reverse

Name: ispB

Synonym: AF1551

Alternate gene names: 11499146

Gene position: 1396924-1396046 (Counterclockwise)

Preceding gene: 11499147

Following gene: 11499144

Centisome position: 64.13

GC content: 45.85

Gene sequence:

>879_bases
GTGATTGATACCTGGGAAGAATACAGGATTTTTGAGGACAGCTTGAAGAAGTTTGTGGAATCGGTAGAGGCTACTCCAAA
AATAAAGAAGGGTCTTGCCTACGCTATAACCGCAGGCGGGAAAAGAAGCAGGCCTGTTATAGTCCTTCTCTCCGCCAAGC
TGTGTGGCGGCGATTATGCTGATGTAATGAACCTTGCGCTCTCAGTTGAGCTAATTCACACCGCAAGCTTGGTTCATGAC
GATGTTATCGATAAGGCTGAAAAGCGAAGAAAGAGGGAAGCCTTGCACAAAAAGTACGACATCTCTCTGGCAATAGTCCT
CGGGGACTGGCTGATTTCCAAGTCGGTGGAGCTGACTTCAAAATACGGGGAGGAGATTATCAGGGACTTCTCGAACGTTG
GAATGATGATGTCTGAGGGCGAGGTTCTTGACATCTACAGCATCAAGGAAAATTTTACCGAAAAGGACTACTTTGAGTGC
ATAGAAAAGAAGACCGCTGCTTTGTTTGCCTACTCCGCAAAGAGCGCTTGTAAAATCGTTTGCGAAGACCGCATTGCCGC
GGATAAACTCTTCGACTACGGCCTGAATCTCGGAATTGCTTACCAAATCGTGGATGACCTGCTCGAGTATCTCAGACTTC
TGGATGACAAGCACTCGGTGTTTGAATCGAGAACGCTTCCGCAGATTTACGAGGAAAAGTACGGGAAGGAGGAGGGTGTT
AACAAGGTCATGGAGAAGATAAGTACCTTCGTTGAGAGGAGCTTAAGCTCTCTGGAATATTTTGATGAAAGTCCGGAGAA
GGAGAAGCTTAGAAAAATCGTCGATTTCATGACCTACGATATGATAAGAAATAAAGCCGAAAAGAAGGCTATTTTCTGA

Upstream 100 bases:

>100_bases
TTCTGGCTGGAGTTCTGATTGCCTGGAGGGAGTGAGTGGATTTTGAGAGTGATAAAAAATCTCGAAAATGTTATAAGTTC
AACACCATGAAAGAACCTGA

Downstream 100 bases:

>100_bases
GCTTTCTGAACAGCTTCTCGTTATTTTCCAGCACCGACTTCCACTCCTTTACTCCGGCACCCTTTAAAATCCTCTCAGCG
GTGGAGTCATTGATTATGTC

Product: octaprenyl-diphosphate synthase (ispB)

Products: NA

Alternate protein names: GGPP synthase; GGPS; (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Farnesyltranstransferase; Geranyltranstransferase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MIDTWEEYRIFEDSLKKFVESVEATPKIKKGLAYAITAGGKRSRPVIVLLSAKLCGGDYADVMNLALSVELIHTASLVHD
DVIDKAEKRRKREALHKKYDISLAIVLGDWLISKSVELTSKYGEEIIRDFSNVGMMMSEGEVLDIYSIKENFTEKDYFEC
IEKKTAALFAYSAKSACKIVCEDRIAADKLFDYGLNLGIAYQIVDDLLEYLRLLDDKHSVFESRTLPQIYEEKYGKEEGV
NKVMEKISTFVERSLSSLEYFDESPEKEKLRKIVDFMTYDMIRNKAEKKAIF

Sequences:

>Translated_292_residues
MIDTWEEYRIFEDSLKKFVESVEATPKIKKGLAYAITAGGKRSRPVIVLLSAKLCGGDYADVMNLALSVELIHTASLVHD
DVIDKAEKRRKREALHKKYDISLAIVLGDWLISKSVELTSKYGEEIIRDFSNVGMMMSEGEVLDIYSIKENFTEKDYFEC
IEKKTAALFAYSAKSACKIVCEDRIAADKLFDYGLNLGIAYQIVDDLLEYLRLLDDKHSVFESRTLPQIYEEKYGKEEGV
NKVMEKISTFVERSLSSLEYFDESPEKEKLRKIVDFMTYDMIRNKAEKKAIF
>Mature_292_residues
MIDTWEEYRIFEDSLKKFVESVEATPKIKKGLAYAITAGGKRSRPVIVLLSAKLCGGDYADVMNLALSVELIHTASLVHD
DVIDKAEKRRKREALHKKYDISLAIVLGDWLISKSVELTSKYGEEIIRDFSNVGMMMSEGEVLDIYSIKENFTEKDYFEC
IEKKTAALFAYSAKSACKIVCEDRIAADKLFDYGLNLGIAYQIVDDLLEYLRLLDDKHSVFESRTLPQIYEEKYGKEEGV
NKVMEKISTFVERSLSSLEYFDESPEKEKLRKIVDFMTYDMIRNKAEKKAIF

Specific function: Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate which is a precursor of the ether-linked lipids [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=222, Percent_Identity=34.6846846846847, Blast_Score=107, Evalue=9e-24,
Organism=Escherichia coli, GI1789578, Length=264, Percent_Identity=28.4090909090909, Blast_Score=110, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17505681, Length=227, Percent_Identity=27.7533039647577, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24651612, Length=223, Percent_Identity=29.1479820627803, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.1; =2.5.1.29; =2.5.1.10 [H]

Molecular weight: Translated: 33540; Mature: 33540

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTWEEYRIFEDSLKKFVESVEATPKIKKGLAYAITAGGKRSRPVIVLLSAKLCGGDYA
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEEECCCCCCCCEEEEEECCCCCCCHH
DVMNLALSVELIHTASLVHDDVIDKAEKRRKREALHKKYDISLAIVLGDWLISKSVELTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHH
KYGEEIIRDFSNVGMMMSEGEVLDIYSIKENFTEKDYFECIEKKTAALFAYSAKSACKIV
HHHHHHHHHHHHCCCEECCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
CEDRIAADKLFDYGLNLGIAYQIVDDLLEYLRLLDDKHSVFESRTLPQIYEEKYGKEEGV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHCCHHHHH
NKVMEKISTFVERSLSSLEYFDESPEKEKLRKIVDFMTYDMIRNKAEKKAIF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDTWEEYRIFEDSLKKFVESVEATPKIKKGLAYAITAGGKRSRPVIVLLSAKLCGGDYA
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEEECCCCCCCCEEEEEECCCCCCCHH
DVMNLALSVELIHTASLVHDDVIDKAEKRRKREALHKKYDISLAIVLGDWLISKSVELTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHH
KYGEEIIRDFSNVGMMMSEGEVLDIYSIKENFTEKDYFECIEKKTAALFAYSAKSACKIV
HHHHHHHHHHHHCCCEECCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
CEDRIAADKLFDYGLNLGIAYQIVDDLLEYLRLLDDKHSVFESRTLPQIYEEKYGKEEGV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHCCHHHHH
NKVMEKISTFVERSLSSLEYFDESPEKEKLRKIVDFMTYDMIRNKAEKKAIF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8182085 [H]