Click here to switch to the map view.

The map label for this gene is vhuA

Identifier: 11498968

GI number: 11498968

Start: 1224163

End: 1225539

Strand: Reverse

Name: vhuA

Synonym: AF1372

Alternate gene names: 11498968

Gene position: 1225539-1224163 (Counterclockwise)

Preceding gene: 11498969

Following gene: 11498967

Centisome position: 56.26

GC content: 51.78

Gene sequence:

>1377_bases
ATGAAAATCGAAATAAATCCCGTTTCACGAATTGAGGGGCATGCTAAGGTAACAATCTTTTTGGATTCCAGCGGAAAGGT
TGAAAATGCCTTCTTTCAGGCTACGGAGTTCAGGGGATACGAGAAAATACTTTCAGGCTTGCCAATGGAGGAGGTGCCGA
GAGTTGTATCGACAATATGCGGAATATGCAGGGCAATCCACTTCACCGCAGCCCTGAAAGCTGCTGATCAGGTTTTTGGT
GTTGAGGCGAGCGATGCGGCAAAGATTGTGAGAGAGATGCTCCTCTGCGCGCACTTCATAGAAGACCACACGCTCTCTCT
GCTCGCCCTTGCCCTTCCTGATTTTCTCTCTCCCGAAGAGAGGAACATTTTTGGTGTAATTAGAAACCTCGGTGAGCTCG
GGAGGGAGCTTCTGAGGAAGAGGGGGTATGCGGTTAGAATTCTGCAGATACTGGGGGGCAGAAACATCCATCCCGTCACA
GCCCTACCCGGGGGGTGGGCGAAAGCTCCGACAGATGAGGAGCTCGGTCTGATAGAGAGGTACTCAAGGGAGCTGCTCGA
CCTCGGTTACAGGATGGTCGAGGTGCTTGAAGCTGCGCTGGACATGGGTGATGGTTTGGAGCTAAGGGCCGCAACGCTTG
GGACGGAGAAGAAAGGCTTAGTGAACCTCTACGATGGTGAGCAGGTGGTGAAGGATGAGGAGGGAAAGGAAGTTCTCAGG
TTCAGCGGGAAGGAGTATGCTGATATCATCACTGAAAAAAGCCTCAGCTGGAGCTACAGCAAGCTGACCGAAATCAAACC
TCTCGGCGAGAAACTTTACGTCGTTGGGCCACTGGCAAGGCTAAAAACGGCCGAGCTAACGCCCAAGGCATCTGAAATTG
TTAAGACTTACGGCGTTAGCAACAATCTTCTCTACAACCACTTTGCGAGGGCTGTGGAGATAATATACGCCGCTGAGAGG
CTGCTTGAACTATCGCAGAGCTACAGGGGTGGTAGGATTCAGAACGAGCCGCAGAAGGTTGCTGGAGAAGGAGTTGGGAT
AGTCGAGGCTCCAAGAGGAACGCTCATTCACCACTACTGGACTGATGAGAAGGGGATCGTTAGGGGCTCAAACCTCATTG
TGGCTACAACTCACAATGTGAGGGCGATAAACCACGTTGTGGGGAAGGTTGCTGAGAGAGCATCAGACACAAGGAATCTG
GCGGAGAAGGTTGAGGTGGCCATAAGGGCCTTTGACCCCTGCATGGCCTGCGCCACTCATGCTGTTAACGGCGTCATTCC
CATTGAAATCCAGATTTTCAGGGATGGGGAGCTGGAGGAAGTTTACAGAAACTATGAGGAAAGTTGTTGTGGGAGTGGGC
AATCCACACATTCCTGA

Upstream 100 bases:

>100_bases
TGACCTACACCAATCTGAGCAAGCTCCTCTACCTCTACACGCTCCCCTCCTCAACTTTGCGGGGGAAGGTCAGGAGGTAC
CACTTGAGAGGTGTGAAGAG

Downstream 100 bases:

>100_bases
GGACTCTTTTGGAGTAAAAGTTGCAGAGATGGCGAAAAAAGCTGGATTTGACGCTTTAGCAGCCTTCCCTCTCGATTTGC
CAGAAATGCTGGAGGGATAC

Product: methylviologen-reducing hydrogenase, subunit alpha (vhuA)

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKIEINPVSRIEGHAKVTIFLDSSGKVENAFFQATEFRGYEKILSGLPMEEVPRVVSTICGICRAIHFTAALKAADQVFG
VEASDAAKIVREMLLCAHFIEDHTLSLLALALPDFLSPEERNIFGVIRNLGELGRELLRKRGYAVRILQILGGRNIHPVT
ALPGGWAKAPTDEELGLIERYSRELLDLGYRMVEVLEAALDMGDGLELRAATLGTEKKGLVNLYDGEQVVKDEEGKEVLR
FSGKEYADIITEKSLSWSYSKLTEIKPLGEKLYVVGPLARLKTAELTPKASEIVKTYGVSNNLLYNHFARAVEIIYAAER
LLELSQSYRGGRIQNEPQKVAGEGVGIVEAPRGTLIHHYWTDEKGIVRGSNLIVATTHNVRAINHVVGKVAERASDTRNL
AEKVEVAIRAFDPCMACATHAVNGVIPIEIQIFRDGELEEVYRNYEESCCGSGQSTHS

Sequences:

>Translated_458_residues
MKIEINPVSRIEGHAKVTIFLDSSGKVENAFFQATEFRGYEKILSGLPMEEVPRVVSTICGICRAIHFTAALKAADQVFG
VEASDAAKIVREMLLCAHFIEDHTLSLLALALPDFLSPEERNIFGVIRNLGELGRELLRKRGYAVRILQILGGRNIHPVT
ALPGGWAKAPTDEELGLIERYSRELLDLGYRMVEVLEAALDMGDGLELRAATLGTEKKGLVNLYDGEQVVKDEEGKEVLR
FSGKEYADIITEKSLSWSYSKLTEIKPLGEKLYVVGPLARLKTAELTPKASEIVKTYGVSNNLLYNHFARAVEIIYAAER
LLELSQSYRGGRIQNEPQKVAGEGVGIVEAPRGTLIHHYWTDEKGIVRGSNLIVATTHNVRAINHVVGKVAERASDTRNL
AEKVEVAIRAFDPCMACATHAVNGVIPIEIQIFRDGELEEVYRNYEESCCGSGQSTHS
>Mature_458_residues
MKIEINPVSRIEGHAKVTIFLDSSGKVENAFFQATEFRGYEKILSGLPMEEVPRVVSTICGICRAIHFTAALKAADQVFG
VEASDAAKIVREMLLCAHFIEDHTLSLLALALPDFLSPEERNIFGVIRNLGELGRELLRKRGYAVRILQILGGRNIHPVT
ALPGGWAKAPTDEELGLIERYSRELLDLGYRMVEVLEAALDMGDGLELRAATLGTEKKGLVNLYDGEQVVKDEEGKEVLR
FSGKEYADIITEKSLSWSYSKLTEIKPLGEKLYVVGPLARLKTAELTPKASEIVKTYGVSNNLLYNHFARAVEIIYAAER
LLELSQSYRGGRIQNEPQKVAGEGVGIVEAPRGTLIHHYWTDEKGIVRGSNLIVATTHNVRAINHVVGKVAERASDTRNL
AEKVEVAIRAFDPCMACATHAVNGVIPIEIQIFRDGELEEVYRNYEESCCGSGQSTHS

Specific function: Part of a complex that provides reducing equivalents for heterodisulfide reductase [H]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=116, Percent_Identity=30.1724137931034, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789368, Length=115, Percent_Identity=29.5652173913043, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.98.1

Molecular weight: Translated: 50587; Mature: 50587

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEINPVSRIEGHAKVTIFLDSSGKVENAFFQATEFRGYEKILSGLPMEEVPRVVSTIC
CEEECCCHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHH
GICRAIHFTAALKAADQVFGVEASDAAKIVREMLLCAHFIEDHTLSLLALALPDFLSPEE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RNIFGVIRNLGELGRELLRKRGYAVRILQILGGRNIHPVTALPGGWAKAPTDEELGLIER
HHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
YSRELLDLGYRMVEVLEAALDMGDGLELRAATLGTEKKGLVNLYDGEQVVKDEEGKEVLR
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCHHHCCCCCHHHHH
FSGKEYADIITEKSLSWSYSKLTEIKPLGEKLYVVGPLARLKTAELTPKASEIVKTYGVS
HCCCHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHCCCCCHHHHHHHHHCCC
NNLLYNHFARAVEIIYAAERLLELSQSYRGGRIQNEPQKVAGEGVGIVEAPRGTLIHHYW
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEECCCCCEEEEEE
TDEKGIVRGSNLIVATTHNVRAINHVVGKVAERASDTRNLAEKVEVAIRAFDPCMACATH
CCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVNGVIPIEIQIFRDGELEEVYRNYEESCCGSGQSTHS
HHCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKIEINPVSRIEGHAKVTIFLDSSGKVENAFFQATEFRGYEKILSGLPMEEVPRVVSTIC
CEEECCCHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHH
GICRAIHFTAALKAADQVFGVEASDAAKIVREMLLCAHFIEDHTLSLLALALPDFLSPEE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RNIFGVIRNLGELGRELLRKRGYAVRILQILGGRNIHPVTALPGGWAKAPTDEELGLIER
HHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
YSRELLDLGYRMVEVLEAALDMGDGLELRAATLGTEKKGLVNLYDGEQVVKDEEGKEVLR
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCHHHCCCCCHHHHH
FSGKEYADIITEKSLSWSYSKLTEIKPLGEKLYVVGPLARLKTAELTPKASEIVKTYGVS
HCCCHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHCCCCCHHHHHHHHHCCC
NNLLYNHFARAVEIIYAAERLLELSQSYRGGRIQNEPQKVAGEGVGIVEAPRGTLIHHYW
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEECCCCCEEEEEE
TDEKGIVRGSNLIVATTHNVRAINHVVGKVAERASDTRNLAEKVEVAIRAFDPCMACATH
CCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVNGVIPIEIQIFRDGELEEVYRNYEESCCGSGQSTHS
HHCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA