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The map label for this gene is hypF

Identifier: 11498962

GI number: 11498962

Start: 1219307

End: 1221529

Strand: Reverse

Name: hypF

Synonym: AF1366

Alternate gene names: 11498962

Gene position: 1221529-1219307 (Counterclockwise)

Preceding gene: 11498967

Following gene: 11498961

Centisome position: 56.07

GC content: 50.2

Gene sequence:

>2223_bases
ATGTTCAAGATTACGGTCAGGGGAGTTGTTCAGGGAGTGGGCTTCAGACCCTTTGTGCACAGAATCGCCAGCAGAATGGG
CTTGAGAGGCTACGTCAGGAACACCGGAGATGGTTCGGTGGAGATTGTGGTTGACAGGAATCCTGAGGAATTTTTGAATA
TTCTGCTAAAAGAGAGGCCACCGCTGGTGGTTATAGAGGATTTCAGCGTCGCCAAAACGAATGGAGAGATTCCCGAAACC
TTCAGAATCGTGGAGAGCGGCGGTAAAAGCCTGCTCTCACTCCCTCCCCCTGACGTTGCCGTTTGCGATAAATGCCTTGA
AGAGGTGCTTGACTCGAAAAACAGAAGGTACCTTTATCCTTTCACCTCCTGCACCGATTGCGGGCCGAGGTTCACGATAA
GCAAATCTCTCCCCTTCGACAGGGAGAACACAAGTCTTGCCGATTTCCCCCTCTGTCCTGACTGCAGGAAGGAATATGGG
GATATTAAGGACAGAAGGTATTTCGCCCAAACAATTGCATGCCCTCGGTGCGGCCCGCAGTACAGATTTGTCTGGAAAGG
CGGAGAGGGTGGTATTGGCGATGCGGCAAACGCTCTCGATTCGGGGAAGATTATAGCGATTAAGGGGATTGGGGGCTACC
ACATAGCCTGCCTTACTGAAGATGATGTGGTTTTAAGGCTTAGAAAGCTGCTGAACCGCCCACAGCAGCCCTTTGCTGTG
ATGGTGAGAGACTTGAGTAGTGCTGAGAGGGTTGCGGAGCTAAGCAATGAGGAGAGGGCCGAGCTGCTCAGCCCGGCGAG
GCCAATTGTTGTTGTGAGAAAAAAGCTCCCCTTCGAGGCAGTAGCTCCAGAGCTCGATACCATTGGCATTATGCTCCCCT
ACACTCCCCTCCACCACCTTCTTTTCCAAGAGCTGAAGGCGGATTATCTGGTGATGACCTCTGCCAACCTCCCGGGAGAG
CCTATGTATATCGATGAGAGCATCATGGAAAAAATGGAGCTTGATGGCTTTCTCGTTCACAACCTCGAAATTGCCAACAG
GGTGGATGATTCCGTTGTAAAGTATGTGGGTGGGAGAAGGCTATTGATTAGGAGGTCGAGGGGCTTTGTTCCCCAGCCAA
TAAAAATTGACATTGGCTTTAATGCAATTGCTGTCGGAGCGGAGCTTTACAATTCCATAGCCCTTCTCAAGGAAGGTAAG
GCGGTCGTTTCGCAGTACATCGGAAACACCGCAAACTTCAGAACCTACAATGAGTTTTTTAAGAGGGCGGTGAAATTCTT
TGCCAGATACATGAACCTTGAAAAAATTGATTACATTATTTCCGACATGCATCCTCTCTTCAACACAACAAATTTTGCCG
AAAAGCTCTCGAAGGAGCTTTCATGCAGGCATATTAAGGTTCAGCACCACTTCGCCCATGCTCTGAGTGTGATGGCGGAG
AAAGGACTTGAGAGGGCGGTGGCGATAACGGTTGATGGGGTTGGATACGGAATGGACGGAAAGGTTTGGGGTGGTGAGGT
TCTCCTTATCGACCTTGAAAAGGGAAGATTCGAAAGAAGGGGCAGGCTTGAAACCTTTGGGCTCATTGGTGGTGACGCAG
CAACATACAGACCTCTGAGAATCGCTGTTTCTCTGCTAAAGCGCCATGAGCTGCTTGAGCACTACTGCAAATATGAAGAC
GTGGAAAGGCTGATTAAGGCCCTTCCACTGGCCGTTAAGACCACCTCAGTGGGTAGATTCCTTGATGCGGTGTCTGCAAT
GCTTGAAGTTTGCCTTGAAAGAACCTACGAGGGTGAACCTGCGATGAAGCTTGAGGCTGCTGCAGAGCCCCACGAAGTGG
AAGTTAAGCCGGAAATTGAGAGAAGAAGGGAAACATGCGTCTACAACTCGCCTTTTGATACAGAGGGGAGGCAGGGGGAG
ATTAAGGTTTTGATGCTGAGGGACTTTTTTACACGCTGCATGGAGAGGTATCTGGAAGGTGAGGGGAGAGGAAAAATAGC
CTACGAGGCCATTGCCTACGTTGCACGGGGTCTGGGAGAGATTGCGCTTGATGCTGCAAGGAACTGCAAGATTCTAATTT
CAGGGGGAGTGGGCTACAACAGGTATTTCCTGAGGGAGGTGGAGAGGGTTTGCGGAGAGATTGTTGTGCCCTCCCTTTAT
CCTCTTGGTGATAATGGAATAAGTCTTGGGCAGATTTACGCCTTGAAAGCTCTGGAGGGGTAA

Upstream 100 bases:

>100_bases
AATCTAAGGACATTTCTGATTATCGCCTCTGCAAAGCTCATTTCATGCACAAAAGGAGTTTTCGCAATATTTTTAAAAGC
TTTTCTTAAGCTCATGATGA

Downstream 100 bases:

>100_bases
AATGTCGGTAATCAGGAAGGAGGATGGAGCTGGAGGAAAGTACATGGTAGAGCTCCTCAAAAAGCACGTTTTTCCAAAAA
TTGCTTCCAGAGCAGGAGAG

Product: hydrogenase expression/formation regulatory protein (hypF)

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 740; Mature: 740

Protein sequence:

>740_residues
MFKITVRGVVQGVGFRPFVHRIASRMGLRGYVRNTGDGSVEIVVDRNPEEFLNILLKERPPLVVIEDFSVAKTNGEIPET
FRIVESGGKSLLSLPPPDVAVCDKCLEEVLDSKNRRYLYPFTSCTDCGPRFTISKSLPFDRENTSLADFPLCPDCRKEYG
DIKDRRYFAQTIACPRCGPQYRFVWKGGEGGIGDAANALDSGKIIAIKGIGGYHIACLTEDDVVLRLRKLLNRPQQPFAV
MVRDLSSAERVAELSNEERAELLSPARPIVVVRKKLPFEAVAPELDTIGIMLPYTPLHHLLFQELKADYLVMTSANLPGE
PMYIDESIMEKMELDGFLVHNLEIANRVDDSVVKYVGGRRLLIRRSRGFVPQPIKIDIGFNAIAVGAELYNSIALLKEGK
AVVSQYIGNTANFRTYNEFFKRAVKFFARYMNLEKIDYIISDMHPLFNTTNFAEKLSKELSCRHIKVQHHFAHALSVMAE
KGLERAVAITVDGVGYGMDGKVWGGEVLLIDLEKGRFERRGRLETFGLIGGDAATYRPLRIAVSLLKRHELLEHYCKYED
VERLIKALPLAVKTTSVGRFLDAVSAMLEVCLERTYEGEPAMKLEAAAEPHEVEVKPEIERRRETCVYNSPFDTEGRQGE
IKVLMLRDFFTRCMERYLEGEGRGKIAYEAIAYVARGLGEIALDAARNCKILISGGVGYNRYFLREVERVCGEIVVPSLY
PLGDNGISLGQIYALKALEG

Sequences:

>Translated_740_residues
MFKITVRGVVQGVGFRPFVHRIASRMGLRGYVRNTGDGSVEIVVDRNPEEFLNILLKERPPLVVIEDFSVAKTNGEIPET
FRIVESGGKSLLSLPPPDVAVCDKCLEEVLDSKNRRYLYPFTSCTDCGPRFTISKSLPFDRENTSLADFPLCPDCRKEYG
DIKDRRYFAQTIACPRCGPQYRFVWKGGEGGIGDAANALDSGKIIAIKGIGGYHIACLTEDDVVLRLRKLLNRPQQPFAV
MVRDLSSAERVAELSNEERAELLSPARPIVVVRKKLPFEAVAPELDTIGIMLPYTPLHHLLFQELKADYLVMTSANLPGE
PMYIDESIMEKMELDGFLVHNLEIANRVDDSVVKYVGGRRLLIRRSRGFVPQPIKIDIGFNAIAVGAELYNSIALLKEGK
AVVSQYIGNTANFRTYNEFFKRAVKFFARYMNLEKIDYIISDMHPLFNTTNFAEKLSKELSCRHIKVQHHFAHALSVMAE
KGLERAVAITVDGVGYGMDGKVWGGEVLLIDLEKGRFERRGRLETFGLIGGDAATYRPLRIAVSLLKRHELLEHYCKYED
VERLIKALPLAVKTTSVGRFLDAVSAMLEVCLERTYEGEPAMKLEAAAEPHEVEVKPEIERRRETCVYNSPFDTEGRQGE
IKVLMLRDFFTRCMERYLEGEGRGKIAYEAIAYVARGLGEIALDAARNCKILISGGVGYNRYFLREVERVCGEIVVPSLY
PLGDNGISLGQIYALKALEG
>Mature_740_residues
MFKITVRGVVQGVGFRPFVHRIASRMGLRGYVRNTGDGSVEIVVDRNPEEFLNILLKERPPLVVIEDFSVAKTNGEIPET
FRIVESGGKSLLSLPPPDVAVCDKCLEEVLDSKNRRYLYPFTSCTDCGPRFTISKSLPFDRENTSLADFPLCPDCRKEYG
DIKDRRYFAQTIACPRCGPQYRFVWKGGEGGIGDAANALDSGKIIAIKGIGGYHIACLTEDDVVLRLRKLLNRPQQPFAV
MVRDLSSAERVAELSNEERAELLSPARPIVVVRKKLPFEAVAPELDTIGIMLPYTPLHHLLFQELKADYLVMTSANLPGE
PMYIDESIMEKMELDGFLVHNLEIANRVDDSVVKYVGGRRLLIRRSRGFVPQPIKIDIGFNAIAVGAELYNSIALLKEGK
AVVSQYIGNTANFRTYNEFFKRAVKFFARYMNLEKIDYIISDMHPLFNTTNFAEKLSKELSCRHIKVQHHFAHALSVMAE
KGLERAVAITVDGVGYGMDGKVWGGEVLLIDLEKGRFERRGRLETFGLIGGDAATYRPLRIAVSLLKRHELLEHYCKYED
VERLIKALPLAVKTTSVGRFLDAVSAMLEVCLERTYEGEPAMKLEAAAEPHEVEVKPEIERRRETCVYNSPFDTEGRQGE
IKVLMLRDFFTRCMERYLEGEGRGKIAYEAIAYVARGLGEIALDAARNCKILISGGVGYNRYFLREVERVCGEIVVPSLY
PLGDNGISLGQIYALKALEG

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=777, Percent_Identity=35.5212355212355, Blast_Score=347, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 83062; Mature: 83062

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKITVRGVVQGVGFRPFVHRIASRMGLRGYVRNTGDGSVEIVVDRNPEEFLNILLKERP
CEEEEEEHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCEEEEEEECCHHHHHHHHHHCCC
PLVVIEDFSVAKTNGEIPETFRIVESGGKSLLSLPPPDVAVCDKCLEEVLDSKNRRYLYP
CEEEEECCEEECCCCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCCEEEEC
FTSCTDCGPRFTISKSLPFDRENTSLADFPLCPDCRKEYGDIKDRRYFAQTIACPRCGPQ
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCC
YRFVWKGGEGGIGDAANALDSGKIIAIKGIGGYHIACLTEDDVVLRLRKLLNRPQQPFAV
EEEEEECCCCCCCHHHHHCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCCHHH
MVRDLSSAERVAELSNEERAELLSPARPIVVVRKKLPFEAVAPELDTIGIMLPYTPLHHL
HHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCHHHHCCCCCCEEEEECCHHHHHH
LFQELKADYLVMTSANLPGEPMYIDESIMEKMELDGFLVHNLEIANRVDDSVVKYVGGRR
HHHHHCCCEEEEEECCCCCCCEEECHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCE
LLIRRSRGFVPQPIKIDIGFNAIAVGAELYNSIALLKEGKAVVSQYIGNTANFRTYNEFF
EEEEECCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHH
KRAVKFFARYMNLEKIDYIISDMHPLFNTTNFAEKLSKELSCRHIKVQHHFAHALSVMAE
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHEEEHHHHHHHHHHHHHH
KGLERAVAITVDGVGYGMDGKVWGGEVLLIDLEKGRFERRGRLETFGLIGGDAATYRPLR
HCCCEEEEEEECCCCCCCCCCEECCEEEEEECCCCCCCCCCCCEEEEEECCCCHHCHHHH
IAVSLLKRHELLEHYCKYEDVERLIKALPLAVKTTSVGRFLDAVSAMLEVCLERTYEGEP
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCC
AMKLEAAAEPHEVEVKPEIERRRETCVYNSPFDTEGRQGEIKVLMLRDFFTRCMERYLEG
CEEEECCCCCCEEECCCHHHHHHHHEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCC
EGRGKIAYEAIAYVARGLGEIALDAARNCKILISGGVGYNRYFLREVERVCGEIVVPSLY
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHEECCCEE
PLGDNGISLGQIYALKALEG
ECCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MFKITVRGVVQGVGFRPFVHRIASRMGLRGYVRNTGDGSVEIVVDRNPEEFLNILLKERP
CEEEEEEHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCEEEEEEECCHHHHHHHHHHCCC
PLVVIEDFSVAKTNGEIPETFRIVESGGKSLLSLPPPDVAVCDKCLEEVLDSKNRRYLYP
CEEEEECCEEECCCCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCCEEEEC
FTSCTDCGPRFTISKSLPFDRENTSLADFPLCPDCRKEYGDIKDRRYFAQTIACPRCGPQ
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCC
YRFVWKGGEGGIGDAANALDSGKIIAIKGIGGYHIACLTEDDVVLRLRKLLNRPQQPFAV
EEEEEECCCCCCCHHHHHCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCCHHH
MVRDLSSAERVAELSNEERAELLSPARPIVVVRKKLPFEAVAPELDTIGIMLPYTPLHHL
HHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCHHHHCCCCCCEEEEECCHHHHHH
LFQELKADYLVMTSANLPGEPMYIDESIMEKMELDGFLVHNLEIANRVDDSVVKYVGGRR
HHHHHCCCEEEEEECCCCCCCEEECHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCE
LLIRRSRGFVPQPIKIDIGFNAIAVGAELYNSIALLKEGKAVVSQYIGNTANFRTYNEFF
EEEEECCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHH
KRAVKFFARYMNLEKIDYIISDMHPLFNTTNFAEKLSKELSCRHIKVQHHFAHALSVMAE
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHEEEHHHHHHHHHHHHHH
KGLERAVAITVDGVGYGMDGKVWGGEVLLIDLEKGRFERRGRLETFGLIGGDAATYRPLR
HCCCEEEEEEECCCCCCCCCCEECCEEEEEECCCCCCCCCCCCEEEEEECCCCHHCHHHH
IAVSLLKRHELLEHYCKYEDVERLIKALPLAVKTTSVGRFLDAVSAMLEVCLERTYEGEP
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCC
AMKLEAAAEPHEVEVKPEIERRRETCVYNSPFDTEGRQGEIKVLMLRDFFTRCMERYLEG
CEEEECCCCCCEEECCCHHHHHHHHEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCC
EGRGKIAYEAIAYVARGLGEIALDAARNCKILISGGVGYNRYFLREVERVCGEIVVPSLY
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHEECCCEE
PLGDNGISLGQIYALKALEG
ECCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]