Click here to switch to the map view.

The map label for this gene is trpB-1

Identifier: 11498839

GI number: 11498839

Start: 1107270

End: 1108577

Strand: Reverse

Name: trpB-1

Synonym: AF1240

Alternate gene names: 11498839

Gene position: 1108577-1107270 (Counterclockwise)

Preceding gene: 11498845

Following gene: 11498838

Centisome position: 50.89

GC content: 54.74

Gene sequence:

>1308_bases
ATGCTCTCATGGTGCATGAAAAAGATAATGCTTGATGAGAGCGAGATGCCGAAGGAGTGGTACAACGTTCTGCCCGATCT
GCCCGAACCTCTCCCTCCACCGCTCCATCCCGCAACGCAGGAGCCTGTAAAGCCAGAAGATTTGGAGCCGATTTTTCCGA
AAGGGCTGATTCAGCAGGAGATGAGTGGCGAGAGGTGGATAAGGATTCCGGAGGATGTAAGGGAAATTTACAGAATCTGG
CGTCCAACACCTCTGGTAAGGGCTGAGAGGCTTGAAAAGGCTCTGAAAACTCCAGCAAGGATTTATTTCAAGTACGAGGG
GGCAAGCCCTCCGGGCAGCCACAAGCCCAACACCGCCGTTGCGCAGGCCTACTACAACGCGAAGGAGGGAGTTGAGAGAC
TCACCACTGAAACCGGAGCGGGACAGTGGGGTTCAGCTTTGTGCTTCGCAACGAAGCTCTTCGAGATGGCGTGCACGGTT
TACATGGTTAAAGTAAGCTTCATGCAGAAGCCCTACAGGAGGGTGATGATGGAGACGTGGGGTGGAGAAGTCATTCCATC
TCCCAGCGACAGAACGGAAGTGGGCAGGAAGATTCTTGCAGAGAATCCCGACACGCCGGGGAGTTTGGGTATAGCCATTA
GCGAGGCCATTGAGGATGCTGCCAAAAACGAGAACACCAAGTACAGCCTTGGAAGCGTTCTGAACCACGTCCTGCTTCAC
CAGACTGTTATTGGCCTTGAAACCAAGGCGCAGCTTGAGAAGGTTGATGAGAAGCCTGACGTGCTGATTGGCTGTGTGGG
TGGTGGTAGCAACTTTGCAGGCCTCACTTACCCCTTCGTTAACGACGCGAAGAATGGTGATCTGGAAATCATCGCTGTTG
AGCCCGCTGCCTGCCCCACGTTGACGGCAGGAGAGTACAAGTACGATTTCGGTGATGTTGCAGGCCTGACACCCCTTCTG
AAGATGTACACCCTCGGCCACGACTTCATCCCACCTCCAATCCACGCTGGTGGGCTTAGGTACCACGGTGACGCTCCAAC
ACTCTGCATGCTTGTCAAGCACGGCGTAATTAAGGCAAGGGCGGTGAAGCAGCTTCCAACATTCGAGGCCGGGTTGCTCT
TCGCCAGAACGGAGGGCATAATCCCCGCTCCGGAAACCAACCACGCAGTGAGGGCGGCGATTGATGAGGCCATTAAGGCG
AGGGAGAACAACGAGGAGAAGGTAATCGTCTTCGGCTTCAGCGGTCACGGATTGCTTGACTTGCAGGCTTACGACGACTA
CCTTGCGGGAAGGCTTGCCGACACTTAG

Upstream 100 bases:

>100_bases
TCGACTTATCAAGCTTCATGCTTCGCTCTCTCCAATTCGTTTTAAATGTTTTCTTGCTGAGAATGTTCCCATTTAATGGG
AAAAAACTTAAATAAAATGA

Downstream 100 bases:

>100_bases
GCCAGTTTTTACAGTCTTTGTCAAAATCCCCTCCAATTTTTATTCAGCTTTTCCCGGGAGAGCTCATGAAGTTCAGAGTG
GAGTTCGACCCAAGGAGATG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MLSWCMKKIMLDESEMPKEWYNVLPDLPEPLPPPLHPATQEPVKPEDLEPIFPKGLIQQEMSGERWIRIPEDVREIYRIW
RPTPLVRAERLEKALKTPARIYFKYEGASPPGSHKPNTAVAQAYYNAKEGVERLTTETGAGQWGSALCFATKLFEMACTV
YMVKVSFMQKPYRRVMMETWGGEVIPSPSDRTEVGRKILAENPDTPGSLGIAISEAIEDAAKNENTKYSLGSVLNHVLLH
QTVIGLETKAQLEKVDEKPDVLIGCVGGGSNFAGLTYPFVNDAKNGDLEIIAVEPAACPTLTAGEYKYDFGDVAGLTPLL
KMYTLGHDFIPPPIHAGGLRYHGDAPTLCMLVKHGVIKARAVKQLPTFEAGLLFARTEGIIPAPETNHAVRAAIDEAIKA
RENNEEKVIVFGFSGHGLLDLQAYDDYLAGRLADT

Sequences:

>Translated_435_residues
MLSWCMKKIMLDESEMPKEWYNVLPDLPEPLPPPLHPATQEPVKPEDLEPIFPKGLIQQEMSGERWIRIPEDVREIYRIW
RPTPLVRAERLEKALKTPARIYFKYEGASPPGSHKPNTAVAQAYYNAKEGVERLTTETGAGQWGSALCFATKLFEMACTV
YMVKVSFMQKPYRRVMMETWGGEVIPSPSDRTEVGRKILAENPDTPGSLGIAISEAIEDAAKNENTKYSLGSVLNHVLLH
QTVIGLETKAQLEKVDEKPDVLIGCVGGGSNFAGLTYPFVNDAKNGDLEIIAVEPAACPTLTAGEYKYDFGDVAGLTPLL
KMYTLGHDFIPPPIHAGGLRYHGDAPTLCMLVKHGVIKARAVKQLPTFEAGLLFARTEGIIPAPETNHAVRAAIDEAIKA
RENNEEKVIVFGFSGHGLLDLQAYDDYLAGRLADT
>Mature_435_residues
MLSWCMKKIMLDESEMPKEWYNVLPDLPEPLPPPLHPATQEPVKPEDLEPIFPKGLIQQEMSGERWIRIPEDVREIYRIW
RPTPLVRAERLEKALKTPARIYFKYEGASPPGSHKPNTAVAQAYYNAKEGVERLTTETGAGQWGSALCFATKLFEMACTV
YMVKVSFMQKPYRRVMMETWGGEVIPSPSDRTEVGRKILAENPDTPGSLGIAISEAIEDAAKNENTKYSLGSVLNHVLLH
QTVIGLETKAQLEKVDEKPDVLIGCVGGGSNFAGLTYPFVNDAKNGDLEIIAVEPAACPTLTAGEYKYDFGDVAGLTPLL
KMYTLGHDFIPPPIHAGGLRYHGDAPTLCMLVKHGVIKARAVKQLPTFEAGLLFARTEGIIPAPETNHAVRAAIDEAIKA
RENNEEKVIVFGFSGHGLLDLQAYDDYLAGRLADT

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=365, Percent_Identity=30.4109589041096, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321412, Length=379, Percent_Identity=30.8707124010554, Blast_Score=121, Evalue=2e-28,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB2_ARCFU (O29028)

Other databases:

- EMBL:   AE000782
- PIR:   G69404
- RefSeq:   NP_070068.1
- ProteinModelPortal:   O29028
- GeneID:   1484464
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1240
- NMPDR:   fig|224325.1.peg.1228
- TIGR:   AF_1240
- HOGENOM:   HBG303148
- OMA:   YTLGHDF
- ProtClustDB:   PRK12391
- BioCyc:   AFUL224325:AF_1240-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026
- TIGRFAMs:   TIGR01415

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 48012; Mature: 48012

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSWCMKKIMLDESEMPKEWYNVLPDLPEPLPPPLHPATQEPVKPEDLEPIFPKGLIQQE
CHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
MSGERWIRIPEDVREIYRIWRPTPLVRAERLEKALKTPARIYFKYEGASPPGSHKPNTAV
HCCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHH
AQAYYNAKEGVERLTTETGAGQWGSALCFATKLFEMACTVYMVKVSFMQKPYRRVMMETW
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGEVIPSPSDRTEVGRKILAENPDTPGSLGIAISEAIEDAAKNENTKYSLGSVLNHVLLH
CCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
QTVIGLETKAQLEKVDEKPDVLIGCVGGGSNFAGLTYPFVNDAKNGDLEIIAVEPAACPT
HHHHCCCHHHHHHHCCCCCCEEEEEECCCCCCCCEECCCCCCCCCCCEEEEEECCCCCCC
LTAGEYKYDFGDVAGLTPLLKMYTLGHDFIPPPIHAGGLRYHGDAPTLCMLVKHGVIKAR
CCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCHHHHH
AVKQLPTFEAGLLFARTEGIIPAPETNHAVRAAIDEAIKARENNEEKVIVFGFSGHGLLD
HHHHCCCHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
LQAYDDYLAGRLADT
EHHHHHHHHHHCCCC
>Mature Secondary Structure
MLSWCMKKIMLDESEMPKEWYNVLPDLPEPLPPPLHPATQEPVKPEDLEPIFPKGLIQQE
CHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
MSGERWIRIPEDVREIYRIWRPTPLVRAERLEKALKTPARIYFKYEGASPPGSHKPNTAV
HCCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHH
AQAYYNAKEGVERLTTETGAGQWGSALCFATKLFEMACTVYMVKVSFMQKPYRRVMMETW
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGEVIPSPSDRTEVGRKILAENPDTPGSLGIAISEAIEDAAKNENTKYSLGSVLNHVLLH
CCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
QTVIGLETKAQLEKVDEKPDVLIGCVGGGSNFAGLTYPFVNDAKNGDLEIIAVEPAACPT
HHHHCCCHHHHHHHCCCCCCEEEEEECCCCCCCCEECCCCCCCCCCCEEEEEECCCCCCC
LTAGEYKYDFGDVAGLTPLLKMYTLGHDFIPPPIHAGGLRYHGDAPTLCMLVKHGVIKAR
CCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCHHHHH
AVKQLPTFEAGLLFARTEGIIPAPETNHAVRAAIDEAIKARENNEEKVIVFGFSGHGLLD
HHHHCCCHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
LQAYDDYLAGRLADT
EHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475