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The map label for this gene is purD

Identifier: 11498757

GI number: 11498757

Start: 1033072

End: 1034484

Strand: Reverse

Name: purD

Synonym: AF1157

Alternate gene names: 11498757

Gene position: 1034484-1033072 (Counterclockwise)

Preceding gene: 11498772

Following gene: 11498754

Centisome position: 47.49

GC content: 51.38

Gene sequence:

>1413_bases
ATGAAGGTTCTGGTGGTCGATGCAGGAGGGAGGGGTAACGCAATTGCTCACGCTTTCTCAAGGAGTGAGCAGGTTAAAGA
AATTTATATAGCTCCCGGAAACGGGGGTAGCGAATTTTTCGAAAAGTGTAAGATTGCCGAGCTTGATGGAAAGAAAATTC
CATCTATAAGGGCAATAGACGAGATAGTGCGGTTCGCAAAGAAATGCGAGGTTGACCTCGCTTACATCGGACCGGAAGAG
CCGCTGAGCCTCGGATTGGTTGACAGACTTGAGGAGGAGGGAATACCTGCCGTAGGGCCGAAAAAGGAGGCCACGATTCT
TGAAGCAAGCAAGTGCTGGGCGAAGGATTTCTTAAAGAGAATTGGAGTTCCCATTCCTGAGTACGCGAACTTCGACAATC
CAGAAGAGGCGAAGGAGTACATAAGGGAGAAGTTCAACAACGGGATAGTGGTGAAGGCAGATGGCCTTGCTGCGGGGAAG
GGTGTTTACGTCTGCGACTCCGTAGAGGAGGCATTAAGGGCGGTGGACGAGATAATGGTTCAGAAGAAGTTCGGAGAGGC
TGGAAACAGGATTGTGGTTGAGGAGAGGCTGAGGGGAATTGAGGTTGCCTTCACCGCCATGACGGACGGCAAAACCGTCA
AGCCGTTCGGCCATGCAAGGGACTACAAGAGGGCTTTCGACAGCGATGACATTGAGGGCTTGAGGGACTTCTACATCGGC
CTCACAAAAAAATTCTACACCAAAGCCCAAATAGAGCAACTCTACAGGGAGGGAAAGCTCATCAACCCCAACACAGGAGG
TATGGGTGCGGTGAGCCCACATCCAGCGGTAACGGAGGAGGTAGAGCAGAGAATCATGGAGATGGTTGTGGAGCCGATAA
TAGAGAACTTTGACAAGGAGTTCAAAGGCGTTCTTTATCCTGTCATAATGCTTGTTGAGGAAAACGGAGAACTAATCCCT
AAGGTTCTTGAGATAAACGTCAGGGACTGCGACCCCGGAGCGGAGGCAAAGCTACCGAGGCTTAAGAGCGATATGGCTGA
GATATCGATGGCTGTGGTGGAGGGTAGGCTGGATGAGGTGGAGATGAGGTTCAGCAGCGATTACTGCGTTGCTGTTTGCG
CGGTCAGCGGAGCTCTGAAGGGCAGAGAAGGGCTGAAGCCCGGCTATCCTGCAGACCACTACACCAGCCAGCCTATAACA
GGAATCGAGGAGGCGATGAAGGAGGCGATAATCTACGCCAACGGCATCGCTAAAACGAATGGATACGTCACAACCGGCGG
AAGAGTTCTGACGGTCGTTGGAATGGGACAAAGCATTGAGGAGGCAAGGAGCAAAGCGTATTCAGCACTTGAAAAAATTA
GCTTTCCCGGAATGCGATACAGAAGGACCATAGGGCTTGATGTTCCCGAGTAA

Upstream 100 bases:

>100_bases
ATTTTTACACAGGTAGAATTTGTAATTAAAAACGAGGATTAATTATATATCCGCCCCTGCTGACCGAAAACGGAAAATAA
TCGATGAGATAAAAAACGGT

Downstream 100 bases:

>100_bases
TTAATGAGGAATTGTTATTTTATACTTATCCGAAATAATAACGGCCTAACTCCAAGCTAACACGGAAAACAATTAAACCA
CTCGCATGAAGTATAATTCC

Product: phosphoribosylamine--glycine ligase (purD)

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKVLVVDAGGRGNAIAHAFSRSEQVKEIYIAPGNGGSEFFEKCKIAELDGKKIPSIRAIDEIVRFAKKCEVDLAYIGPEE
PLSLGLVDRLEEEGIPAVGPKKEATILEASKCWAKDFLKRIGVPIPEYANFDNPEEAKEYIREKFNNGIVVKADGLAAGK
GVYVCDSVEEALRAVDEIMVQKKFGEAGNRIVVEERLRGIEVAFTAMTDGKTVKPFGHARDYKRAFDSDDIEGLRDFYIG
LTKKFYTKAQIEQLYREGKLINPNTGGMGAVSPHPAVTEEVEQRIMEMVVEPIIENFDKEFKGVLYPVIMLVEENGELIP
KVLEINVRDCDPGAEAKLPRLKSDMAEISMAVVEGRLDEVEMRFSSDYCVAVCAVSGALKGREGLKPGYPADHYTSQPIT
GIEEAMKEAIIYANGIAKTNGYVTTGGRVLTVVGMGQSIEEARSKAYSALEKISFPGMRYRRTIGLDVPE

Sequences:

>Translated_470_residues
MKVLVVDAGGRGNAIAHAFSRSEQVKEIYIAPGNGGSEFFEKCKIAELDGKKIPSIRAIDEIVRFAKKCEVDLAYIGPEE
PLSLGLVDRLEEEGIPAVGPKKEATILEASKCWAKDFLKRIGVPIPEYANFDNPEEAKEYIREKFNNGIVVKADGLAAGK
GVYVCDSVEEALRAVDEIMVQKKFGEAGNRIVVEERLRGIEVAFTAMTDGKTVKPFGHARDYKRAFDSDDIEGLRDFYIG
LTKKFYTKAQIEQLYREGKLINPNTGGMGAVSPHPAVTEEVEQRIMEMVVEPIIENFDKEFKGVLYPVIMLVEENGELIP
KVLEINVRDCDPGAEAKLPRLKSDMAEISMAVVEGRLDEVEMRFSSDYCVAVCAVSGALKGREGLKPGYPADHYTSQPIT
GIEEAMKEAIIYANGIAKTNGYVTTGGRVLTVVGMGQSIEEARSKAYSALEKISFPGMRYRRTIGLDVPE
>Mature_470_residues
MKVLVVDAGGRGNAIAHAFSRSEQVKEIYIAPGNGGSEFFEKCKIAELDGKKIPSIRAIDEIVRFAKKCEVDLAYIGPEE
PLSLGLVDRLEEEGIPAVGPKKEATILEASKCWAKDFLKRIGVPIPEYANFDNPEEAKEYIREKFNNGIVVKADGLAAGK
GVYVCDSVEEALRAVDEIMVQKKFGEAGNRIVVEERLRGIEVAFTAMTDGKTVKPFGHARDYKRAFDSDDIEGLRDFYIG
LTKKFYTKAQIEQLYREGKLINPNTGGMGAVSPHPAVTEEVEQRIMEMVVEPIIENFDKEFKGVLYPVIMLVEENGELIP
KVLEINVRDCDPGAEAKLPRLKSDMAEISMAVVEGRLDEVEMRFSSDYCVAVCAVSGALKGREGLKPGYPADHYTSQPIT
GIEEAMKEAIIYANGIAKTNGYVTTGGRVLTVVGMGQSIEEARSKAYSALEKISFPGMRYRRTIGLDVPE

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=471, Percent_Identity=35.6687898089172, Blast_Score=259, Evalue=4e-69,
Organism=Homo sapiens, GI4503915, Length=471, Percent_Identity=35.6687898089172, Blast_Score=259, Evalue=5e-69,
Organism=Homo sapiens, GI209869995, Length=471, Percent_Identity=35.6687898089172, Blast_Score=259, Evalue=5e-69,
Organism=Homo sapiens, GI209869993, Length=471, Percent_Identity=35.6687898089172, Blast_Score=259, Evalue=5e-69,
Organism=Escherichia coli, GI1790438, Length=472, Percent_Identity=35.8050847457627, Blast_Score=268, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI17567511, Length=474, Percent_Identity=32.2784810126582, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321203, Length=476, Percent_Identity=31.3025210084034, Blast_Score=233, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24582400, Length=471, Percent_Identity=35.8811040339703, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI62471619, Length=471, Percent_Identity=35.8811040339703, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_ARCFU (O29108)

Other databases:

- EMBL:   AE000782
- PIR:   D69394
- RefSeq:   NP_069986.1
- ProteinModelPortal:   O29108
- SMR:   O29108
- GeneID:   1484381
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1157
- NMPDR:   fig|224325.1.peg.1146
- TIGR:   AF_1157
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   CLSK541657
- BioCyc:   AFUL224325:AF_1157-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 51751; Mature: 51751

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVVDAGGRGNAIAHAFSRSEQVKEIYIAPGNGGSEFFEKCKIAELDGKKIPSIRAID
CEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCEEECCCCCCCCHHHHH
EIVRFAKKCEVDLAYIGPEEPLSLGLVDRLEEEGIPAVGPKKEATILEASKCWAKDFLKR
HHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IGVPIPEYANFDNPEEAKEYIREKFNNGIVVKADGLAAGKGVYVCDSVEEALRAVDEIMV
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHH
QKKFGEAGNRIVVEERLRGIEVAFTAMTDGKTVKPFGHARDYKRAFDSDDIEGLRDFYIG
HHHHCCCCCEEEEHHHHCCEEEEEEEECCCCEECCCCCHHHHHHHCCCCHHHHHHHHHHH
LTKKFYTKAQIEQLYREGKLINPNTGGMGAVSPHPAVTEEVEQRIMEMVVEPIIENFDKE
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FKGVLYPVIMLVEENGELIPKVLEINVRDCDPGAEAKLPRLKSDMAEISMAVVEGRLDEV
HHHHHEEHHEEECCCCCCCCHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHH
EMRFSSDYCVAVCAVSGALKGREGLKPGYPADHYTSQPITGIEEAMKEAIIYANGIAKTN
HHHCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
GYVTTGGRVLTVVGMGQSIEEARSKAYSALEKISFPGMRYRRTIGLDVPE
CEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MKVLVVDAGGRGNAIAHAFSRSEQVKEIYIAPGNGGSEFFEKCKIAELDGKKIPSIRAID
CEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCEEECCCCCCCCHHHHH
EIVRFAKKCEVDLAYIGPEEPLSLGLVDRLEEEGIPAVGPKKEATILEASKCWAKDFLKR
HHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IGVPIPEYANFDNPEEAKEYIREKFNNGIVVKADGLAAGKGVYVCDSVEEALRAVDEIMV
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHH
QKKFGEAGNRIVVEERLRGIEVAFTAMTDGKTVKPFGHARDYKRAFDSDDIEGLRDFYIG
HHHHCCCCCEEEEHHHHCCEEEEEEEECCCCEECCCCCHHHHHHHCCCCHHHHHHHHHHH
LTKKFYTKAQIEQLYREGKLINPNTGGMGAVSPHPAVTEEVEQRIMEMVVEPIIENFDKE
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FKGVLYPVIMLVEENGELIPKVLEINVRDCDPGAEAKLPRLKSDMAEISMAVVEGRLDEV
HHHHHEEHHEEECCCCCCCCHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHH
EMRFSSDYCVAVCAVSGALKGREGLKPGYPADHYTSQPITGIEEAMKEAIIYANGIAKTN
HHHCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
GYVTTGGRVLTVVGMGQSIEEARSKAYSALEKISFPGMRYRRTIGLDVPE
CEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475