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The map label for this gene is gspE-4

Identifier: 11498654

GI number: 11498654

Start: 956493

End: 958379

Strand: Reverse

Name: gspE-4

Synonym: AF1049

Alternate gene names: 11498654

Gene position: 958379-956493 (Counterclockwise)

Preceding gene: 11498655

Following gene: 11498653

Centisome position: 43.99

GC content: 49.97

Gene sequence:

>1887_bases
ATGGGAAGCGAGGTTGACAAGCTCGATTTCAGTTTGAAGCCGAAGGGATTGAAGCAGGGAGTTGTTGCCAACTTCCCGTT
CAAGCCGAGAGTGCTTGAGGAGGACCACGACCACTCCAATTTAGCTTCGTGCGGAATTTACAAGCTCCTCCCCGACAGAA
TGAAGAAGGAGGTTGAGAAGAACCTTCACCTTCTTGAGTACCTCCATATCCTCCCCCTCGACAAAATCGGAATTCCGAAA
TTCGCTCCGAAGCTGGACAGGAAAAAGCACGGAGACCTCGACAGGCCAAATCTCATTTATCCCGCCGACGAGCAGATTTA
CATTCACGTTTACCCCGACAAAAACGACATGAGGAACTTCTACATCCCAATCGAACCAACCCTGCTTACGGGTGTTGACG
AACTCCTCAAAGAGGTTGAAATAAGGCTTGTAGACTACATAACGGATATAGACTTCGACCCAGAGGATAATGAGGAGAAG
ATAAAGATTCTGAAAGAGGCGCTCAGGGAGGTCTGCGAAATCGTTGATAAGGAAGAGTCCTTTATGGATGATAGCAGGGG
CGAGGTTAAGCTGACGCCCAAATTCAGGTTTCCCTTCAGGCTGCCTTTTTGGAAGAACGGGAAGAATGGAAGCAGAGCTG
TGATGGGAAAGCTGAAGGTTACCAAAGAGCAGTTCAAGGCTTTAGAGTATGCTTTGATAAGGGATAAAGTGGGACTGGGT
GTGCTTGAGCCCTACATCAGGGACAAGTACATAGAGGACATTTCCTGCAGCGGTCTCGGGCCAATATTCGTTGAGCACAA
GATTTTCAAGGGGCTCAAAAGCGTTATCGAGTTCAGAGATGAGGAAACGCTGAACAAGTTCATAATCCGGCTGGCTGAGA
GGATCGGAAAGCCTGTCACCTACAAAGACCCAATCGTTGACGCTACGCTGCCCGACGGGAGCAGAATTAACATAGTCTTT
GGCAACGACATCAGCAAGAACGGCAGCAACTTCACCATCAGAAAGTTCAACGAAACGCCCTTCAGCGTTCTTCAGCTAAT
CGAGTTCGGCTCCCTCGACTACATGATTGCCGGCTACTTATGGCTTCTCATCAGTGAGGGGATGAGCGGCTTTATCTGCG
GTGAAACTGCCAGCGGTAAGACAACGTTGCTGAATGCCATTACAGCCTTCATAAGGCCTGAGGCTAAGGTCGTTTCAATT
GAGGACACTCCGGAGCTGCAGGTTCCGCACAAGAACTGGACGAGGGAGGTTACGAGAGGCTCAACGAGGGCAGGAGGTAT
TGGTGAGGGTGGTGGTAGCGACGTTACGATGTTCGATTTGCTCAAAGCAGCATTGAGGCAGAGGCCCAATTACATCCTGG
TTGGTGAGATTAGAGGTGTTGAGGGGAACATTGCATTCCAGGCCATGCAGACAGGCCACCCGGTCATGTCTACCTTCCAC
GCAGCCACGGTTGAGAAGCTGATTCAGAGGCTTACTACAGAACCCATCAGCGTGCCCAAAACCTTCATCGACAACCTGAA
CTTTGTCGTCATACAGAGTGCGGTAAGAAGGCCGGATGGGAAGCTCGTTAGAAGGGTTCTGAGCGTCAGCGAGATTGTTG
GCTACAACCCGCAGAAGGGGGGAGTGTCCTTCATTGAGGTTTTTCAGTGGGACCCTGTTACAGATACGCACGTTTTCACC
GGCTACGGAAGCTCCTACCTCCTTGAGCAGAAAATTGCGACGAGGCTGGGAATTCCACCGAACAAGAAGAAGCTGATTTA
CGAGGAGGTTGAGAAGAGGGCGAAAATTCTGAAGAAGCTCCACGAGCAGGGAGTTACTGACTTCTACGAGTTTTTCAAGT
CCATATCGAAGATTTACAAGAGCGGATTGCTTGCTATCAAGGTGTAG

Upstream 100 bases:

>100_bases
TTTGGTCACAAGGTGCAGTGTAATCCCAAAAATGAAAGCAAAGGTTAATAGCAACATCAGTTGATAGTAGTAATAGTTAT
TATAATGAAGGGGGGAGGAT

Downstream 100 bases:

>100_bases
CGTATGGAGGTTAAAACGGCAAAGGTTCCTAAGCTCTCGCTCGGATTCGGGAGGTTTGGGAAGGTACTTGATGTTCTGAG
GAGCAGGGGAAAGGACCTTC

Product: type II secretion system protein (gspE-4)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MGSEVDKLDFSLKPKGLKQGVVANFPFKPRVLEEDHDHSNLASCGIYKLLPDRMKKEVEKNLHLLEYLHILPLDKIGIPK
FAPKLDRKKHGDLDRPNLIYPADEQIYIHVYPDKNDMRNFYIPIEPTLLTGVDELLKEVEIRLVDYITDIDFDPEDNEEK
IKILKEALREVCEIVDKEESFMDDSRGEVKLTPKFRFPFRLPFWKNGKNGSRAVMGKLKVTKEQFKALEYALIRDKVGLG
VLEPYIRDKYIEDISCSGLGPIFVEHKIFKGLKSVIEFRDEETLNKFIIRLAERIGKPVTYKDPIVDATLPDGSRINIVF
GNDISKNGSNFTIRKFNETPFSVLQLIEFGSLDYMIAGYLWLLISEGMSGFICGETASGKTTLLNAITAFIRPEAKVVSI
EDTPELQVPHKNWTREVTRGSTRAGGIGEGGGSDVTMFDLLKAALRQRPNYILVGEIRGVEGNIAFQAMQTGHPVMSTFH
AATVEKLIQRLTTEPISVPKTFIDNLNFVVIQSAVRRPDGKLVRRVLSVSEIVGYNPQKGGVSFIEVFQWDPVTDTHVFT
GYGSSYLLEQKIATRLGIPPNKKKLIYEEVEKRAKILKKLHEQGVTDFYEFFKSISKIYKSGLLAIKV

Sequences:

>Translated_628_residues
MGSEVDKLDFSLKPKGLKQGVVANFPFKPRVLEEDHDHSNLASCGIYKLLPDRMKKEVEKNLHLLEYLHILPLDKIGIPK
FAPKLDRKKHGDLDRPNLIYPADEQIYIHVYPDKNDMRNFYIPIEPTLLTGVDELLKEVEIRLVDYITDIDFDPEDNEEK
IKILKEALREVCEIVDKEESFMDDSRGEVKLTPKFRFPFRLPFWKNGKNGSRAVMGKLKVTKEQFKALEYALIRDKVGLG
VLEPYIRDKYIEDISCSGLGPIFVEHKIFKGLKSVIEFRDEETLNKFIIRLAERIGKPVTYKDPIVDATLPDGSRINIVF
GNDISKNGSNFTIRKFNETPFSVLQLIEFGSLDYMIAGYLWLLISEGMSGFICGETASGKTTLLNAITAFIRPEAKVVSI
EDTPELQVPHKNWTREVTRGSTRAGGIGEGGGSDVTMFDLLKAALRQRPNYILVGEIRGVEGNIAFQAMQTGHPVMSTFH
AATVEKLIQRLTTEPISVPKTFIDNLNFVVIQSAVRRPDGKLVRRVLSVSEIVGYNPQKGGVSFIEVFQWDPVTDTHVFT
GYGSSYLLEQKIATRLGIPPNKKKLIYEEVEKRAKILKKLHEQGVTDFYEFFKSISKIYKSGLLAIKV
>Mature_627_residues
GSEVDKLDFSLKPKGLKQGVVANFPFKPRVLEEDHDHSNLASCGIYKLLPDRMKKEVEKNLHLLEYLHILPLDKIGIPKF
APKLDRKKHGDLDRPNLIYPADEQIYIHVYPDKNDMRNFYIPIEPTLLTGVDELLKEVEIRLVDYITDIDFDPEDNEEKI
KILKEALREVCEIVDKEESFMDDSRGEVKLTPKFRFPFRLPFWKNGKNGSRAVMGKLKVTKEQFKALEYALIRDKVGLGV
LEPYIRDKYIEDISCSGLGPIFVEHKIFKGLKSVIEFRDEETLNKFIIRLAERIGKPVTYKDPIVDATLPDGSRINIVFG
NDISKNGSNFTIRKFNETPFSVLQLIEFGSLDYMIAGYLWLLISEGMSGFICGETASGKTTLLNAITAFIRPEAKVVSIE
DTPELQVPHKNWTREVTRGSTRAGGIGEGGGSDVTMFDLLKAALRQRPNYILVGEIRGVEGNIAFQAMQTGHPVMSTFHA
ATVEKLIQRLTTEPISVPKTFIDNLNFVVIQSAVRRPDGKLVRRVLSVSEIVGYNPQKGGVSFIEVFQWDPVTDTHVFTG
YGSSYLLEQKIATRLGIPPNKKKLIYEEVEKRAKILKKLHEQGVTDFYEFFKSISKIYKSGLLAIKV

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG0630

COG function: function code NU; Type IV secretory pathway, VirB11 components, and related ATPases involved in archaeal flagella biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=245, Percent_Identity=26.1224489795918, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786296, Length=264, Percent_Identity=28.030303030303, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 71248; Mature: 71117

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSEVDKLDFSLKPKGLKQGVVANFPFKPRVLEEDHDHSNLASCGIYKLLPDRMKKEVEK
CCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
NLHLLEYLHILPLDKIGIPKFAPKLDRKKHGDLDRPNLIYPADEQIYIHVYPDKNDMRNF
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCEECCCCCEEEEEEECCCCCCCEE
YIPIEPTLLTGVDELLKEVEIRLVDYITDIDFDPEDNEEKIKILKEALREVCEIVDKEES
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHH
FMDDSRGEVKLTPKFRFPFRLPFWKNGKNGSRAVMGKLKVTKEQFKALEYALIRDKVGLG
HHCCCCCCEEEEEEEECCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VLEPYIRDKYIEDISCSGLGPIFVEHKIFKGLKSVIEFRDEETLNKFIIRLAERIGKPVT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
YKDPIVDATLPDGSRINIVFGNDISKNGSNFTIRKFNETPFSVLQLIEFGSLDYMIAGYL
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHH
WLLISEGMSGFICGETASGKTTLLNAITAFIRPEAKVVSIEDTPELQVPHKNWTREVTRG
HHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHCC
STRAGGIGEGGGSDVTMFDLLKAALRQRPNYILVGEIRGVEGNIAFQAMQTGHPVMSTFH
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHH
AATVEKLIQRLTTEPISVPKTFIDNLNFVVIQSAVRRPDGKLVRRVLSVSEIVGYNPQKG
HHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCCC
GVSFIEVFQWDPVTDTHVFTGYGSSYLLEQKIATRLGIPPNKKKLIYEEVEKRAKILKKL
CCEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
HEQGVTDFYEFFKSISKIYKSGLLAIKV
HHCCCHHHHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
GSEVDKLDFSLKPKGLKQGVVANFPFKPRVLEEDHDHSNLASCGIYKLLPDRMKKEVEK
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
NLHLLEYLHILPLDKIGIPKFAPKLDRKKHGDLDRPNLIYPADEQIYIHVYPDKNDMRNF
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCEECCCCCEEEEEEECCCCCCCEE
YIPIEPTLLTGVDELLKEVEIRLVDYITDIDFDPEDNEEKIKILKEALREVCEIVDKEES
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHH
FMDDSRGEVKLTPKFRFPFRLPFWKNGKNGSRAVMGKLKVTKEQFKALEYALIRDKVGLG
HHCCCCCCEEEEEEEECCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VLEPYIRDKYIEDISCSGLGPIFVEHKIFKGLKSVIEFRDEETLNKFIIRLAERIGKPVT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
YKDPIVDATLPDGSRINIVFGNDISKNGSNFTIRKFNETPFSVLQLIEFGSLDYMIAGYL
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHH
WLLISEGMSGFICGETASGKTTLLNAITAFIRPEAKVVSIEDTPELQVPHKNWTREVTRG
HHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHCC
STRAGGIGEGGGSDVTMFDLLKAALRQRPNYILVGEIRGVEGNIAFQAMQTGHPVMSTFH
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHH
AATVEKLIQRLTTEPISVPKTFIDNLNFVVIQSAVRRPDGKLVRRVLSVSEIVGYNPQKG
HHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCCC
GVSFIEVFQWDPVTDTHVFTGYGSSYLLEQKIATRLGIPPNKKKLIYEEVEKRAKILKKL
CCEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
HEQGVTDFYEFFKSISKIYKSGLLAIKV
HHCCCHHHHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]