BacMap

Definition	Archaeoglobus fulgidus DSM 4304, complete genome.
Accession	NC_000917
Length	2,178,400

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The map label for this gene is fad-3

Identifier: 11498568

GI number: 11498568

Start: 863392

End: 864171

Strand: Reverse

Name: fad-3

Synonym: AF0963

Alternate gene names: 11498568

Gene position: 864171-863392 (Counterclockwise)

Preceding gene: 11498569

Following gene: 11498567

Centisome position: 39.67

GC content: 52.05

Gene sequence:

>780_bases
ATGGCTGCAGTTGAATACAGAGAGGAAGACGGAGTTCTGTGGGTGAAGTTCAACCGTCCCGAAGCGCTCAACGCTATAAA
CAAGGACTTCGTTAAAGGGCTGAGAGAAGTCGTTGATTATGCGAGGAACAACAAGACTGTGAGAGTTATCGTCCTCACTG
GAGAGGGGAAGGCCTTCTGTGCTGGAGCGGACATAAAGATGTTCTCTGAATCCTCCCATTTTGTTGCGAGAAGCACAATT
GAGGAGCTCGGCAAAGTTCTTGAGGAGATGGAGGATTTGGAGGTGCCGGTGATAGCAGCCATAAACGGCTTTGCCCTTGG
TGGGGGCTGTGAGATTGCGATGGCGTGTGATATTATAATAGCAAGCGAGAGGGCGAGCTTCGGTCAGCCTGAAATTAACC
TCGGCATCATTCCCGGAGCGGGAGGAACGCAGAGGCTTGCCAGGATAGTGGGCTGGAAGAAGGCCATGGAGCTCTGCCTC
ACGGGAGAGAGAATCTCCGCAGAGGAAGCCTACAGGCTCGGCTTGGTAAATAAGGTTGTGGAGCACGATAAGTTGATGGA
TGAAGCAAAAAAGATGGCAGAGGTCATCAAGAGCAAGTCTCCCTACGCAGTAATGCTGGTTAAGCAGGCCGTGAACAGGG
GCTTCAAGATGGGGCTGAGAGATGGTATAATGTATGAGAGGGACCTCTTCGCCCTCTCCTTCTCATCTCCCGATGCGGAA
GAGGGGATAAAGGCCTTCGTGGAGAAAAGGAAAGCTGACTTCAGGAGGGAGAGGGAGTGA

Upstream 100 bases:

>100_bases
ATCAATCTGATTCGCCTCGCTCCAGTGACGCAGCAGATGGCGCTGAACTTCATAGCGGAGCACATCCTCGGCATGCCGAA
GAGCTACAGGACGTGAAATT

Downstream 100 bases:

>100_bases
AAGATTTTAGAGTTGTATTTTCCCTTATTTTATTGACTGTGTTTATCCTTGGTTGCACACAACCCGCTCAACCTACCCAG
CCCCAACCTGAGGAGAAAAT

Product: enoyl-CoA hydratase (fad-3)

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MAAVEYREEDGVLWVKFNRPEALNAINKDFVKGLREVVDYARNNKTVRVIVLTGEGKAFCAGADIKMFSESSHFVARSTI
EELGKVLEEMEDLEVPVIAAINGFALGGGCEIAMACDIIIASERASFGQPEINLGIIPGAGGTQRLARIVGWKKAMELCL
TGERISAEEAYRLGLVNKVVEHDKLMDEAKKMAEVIKSKSPYAVMLVKQAVNRGFKMGLRDGIMYERDLFALSFSSPDAE
EGIKAFVEKRKADFRRERE

Sequences:

>Translated_259_residues
MAAVEYREEDGVLWVKFNRPEALNAINKDFVKGLREVVDYARNNKTVRVIVLTGEGKAFCAGADIKMFSESSHFVARSTI
EELGKVLEEMEDLEVPVIAAINGFALGGGCEIAMACDIIIASERASFGQPEINLGIIPGAGGTQRLARIVGWKKAMELCL
TGERISAEEAYRLGLVNKVVEHDKLMDEAKKMAEVIKSKSPYAVMLVKQAVNRGFKMGLRDGIMYERDLFALSFSSPDAE
EGIKAFVEKRKADFRRERE
>Mature_258_residues
AAVEYREEDGVLWVKFNRPEALNAINKDFVKGLREVVDYARNNKTVRVIVLTGEGKAFCAGADIKMFSESSHFVARSTIE
ELGKVLEEMEDLEVPVIAAINGFALGGGCEIAMACDIIIASERASFGQPEINLGIIPGAGGTQRLARIVGWKKAMELCLT
GERISAEEAYRLGLVNKVVEHDKLMDEAKKMAEVIKSKSPYAVMLVKQAVNRGFKMGLRDGIMYERDLFALSFSSPDAEE
GIKAFVEKRKADFRRERE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=243, Percent_Identity=46.9135802469136, Blast_Score=212, Evalue=2e-55,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=31.3492063492063, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=35.6862745098039, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI68989263, Length=196, Percent_Identity=37.7551020408163, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI150378541, Length=251, Percent_Identity=33.4661354581673, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI20127408, Length=183, Percent_Identity=35.5191256830601, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI70995211, Length=230, Percent_Identity=32.6086956521739, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI37594471, Length=175, Percent_Identity=29.7142857142857, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI37594469, Length=175, Percent_Identity=29.7142857142857, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI45643119, Length=213, Percent_Identity=27.6995305164319, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI260275230, Length=213, Percent_Identity=27.6995305164319, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI260274832, Length=213, Percent_Identity=27.6995305164319, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI157694516, Length=247, Percent_Identity=25.1012145748988, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI213417737, Length=247, Percent_Identity=25.1012145748988, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI261878539, Length=96, Percent_Identity=42.7083333333333, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI25453481, Length=263, Percent_Identity=22.4334600760456, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538573, Length=263, Percent_Identity=22.4334600760456, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221136756, Length=253, Percent_Identity=22.5296442687747, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221136753, Length=253, Percent_Identity=22.5296442687747, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221307494, Length=253, Percent_Identity=22.5296442687747, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI116235487, Length=166, Percent_Identity=28.9156626506024, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI4757968, Length=265, Percent_Identity=22.6415094339623, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI49355787, Length=265, Percent_Identity=22.6415094339623, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI157694520, Length=156, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI4757966, Length=228, Percent_Identity=22.8070175438596, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI51538581, Length=228, Percent_Identity=22.8070175438596, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1787659, Length=244, Percent_Identity=39.7540983606557, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI221142681, Length=244, Percent_Identity=37.2950819672131, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=34.6456692913386, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788682, Length=190, Percent_Identity=35.2631578947368, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1788597, Length=256, Percent_Identity=32.8125, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1790281, Length=186, Percent_Identity=36.0215053763441, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI87082183, Length=244, Percent_Identity=29.5081967213115, Blast_Score=107, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=45.6, Blast_Score=221, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=42.9718875502008, Blast_Score=195, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=38.6666666666667, Blast_Score=154, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17536985, Length=258, Percent_Identity=29.0697674418605, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17558304, Length=175, Percent_Identity=36, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=249, Percent_Identity=28.5140562248996, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17534483, Length=230, Percent_Identity=31.304347826087, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17540306, Length=214, Percent_Identity=31.7757009345794, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=28.1746031746032, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25144276, Length=187, Percent_Identity=32.620320855615, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17549921, Length=204, Percent_Identity=30.8823529411765, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=187, Percent_Identity=32.620320855615, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=187, Percent_Identity=32.620320855615, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25144157, Length=190, Percent_Identity=29.4736842105263, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=190, Percent_Identity=29.4736842105263, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=185, Percent_Identity=29.1891891891892, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=208, Percent_Identity=28.3653846153846, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=48.780487804878, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=48.780487804878, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=38.0952380952381, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=27.5590551181102, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI19920382, Length=226, Percent_Identity=31.858407079646, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24650670, Length=250, Percent_Identity=30.4, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI28571730, Length=223, Percent_Identity=28.6995515695067, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28571729, Length=223, Percent_Identity=28.6995515695067, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21357171, Length=255, Percent_Identity=26.2745098039216, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24654903, Length=182, Percent_Identity=31.8681318681319, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19921000, Length=178, Percent_Identity=35.3932584269663, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583077, Length=178, Percent_Identity=35.3932584269663, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583079, Length=178, Percent_Identity=35.3932584269663, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583165, Length=255, Percent_Identity=22.7450980392157, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921018, Length=265, Percent_Identity=26.0377358490566, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28701; Mature: 28569

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVEYREEDGVLWVKFNRPEALNAINKDFVKGLREVVDYARNNKTVRVIVLTGEGKAFC
CCCCCEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEE
AGADIKMFSESSHFVARSTIEELGKVLEEMEDLEVPVIAAINGFALGGGCEIAMACDIII
ECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCEEEEEEEEEE
ASERASFGQPEINLGIIPGAGGTQRLARIVGWKKAMELCLTGERISAEEAYRLGLVNKVV
ECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
EHDKLMDEAKKMAEVIKSKSPYAVMLVKQAVNRGFKMGLRDGIMYERDLFALSFSSPDAE
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCHHCCCEEECCEEEEECCCCCHH
EGIKAFVEKRKADFRRERE
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAVEYREEDGVLWVKFNRPEALNAINKDFVKGLREVVDYARNNKTVRVIVLTGEGKAFC
CCCCEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEE
AGADIKMFSESSHFVARSTIEELGKVLEEMEDLEVPVIAAINGFALGGGCEIAMACDIII
ECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCEEEEEEEEEE
ASERASFGQPEINLGIIPGAGGTQRLARIVGWKKAMELCLTGERISAEEAYRLGLVNKVV
ECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
EHDKLMDEAKKMAEVIKSKSPYAVMLVKQAVNRGFKMGLRDGIMYERDLFALSFSSPDAE
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCHHCCCEEECCEEEEECCCCCHH
EGIKAFVEKRKADFRRERE
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA