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The map label for this gene is top6A

Identifier: 11498545

GI number: 11498545

Start: 840081

End: 841163

Strand: Reverse

Name: top6A

Synonym: AF0940

Alternate gene names: 11498545

Gene position: 841163-840081 (Counterclockwise)

Preceding gene: 11498549

Following gene: 11498534

Centisome position: 38.61

GC content: 51.8

Gene sequence:

>1083_bases
ATGAAAGAGATAGAGCGGAGATGCTTGCGGGCTTTAATCGGTATAGTTCAGAACATATACGACCAGATGAAGGCTGGGCA
GGTTCCCGAGCTTCACATTGCAACGAGAACCAAGTACAACATCGAGTTTAATGAGGAAAGTGAGGTTTGGGTTTACGGTG
ACAGGAAATCCGTAAGGTCGGCTAAAACCGTTAAGGGTGCGTACAGAATCCTCAAGATGACGTATGTTATCGGCTTCCTC
AAGGAGCAGCTTAACCTCAACAAGTCCTCAACCCTCAGAGAGCTTTACTACATCTCTGAGGGCTGGGGGGCGGCGAAGTT
TGAAGAGCAGCCGGAGAGCGACAGGCTTGTTGAGGACCTCGAAATTCTTACGAACTTCCAGAGGGAGCACTTTCACATCC
GCCCTGAGGAAGATGGTGCCACCGTCATCGGCCCTCTGAGAGTCAGGGAAGAGACAAGAAGAGGAGTGAGGGAGATACAC
TGCCAGGATGATGTCGGAGAGGGAGGTTACCAGATTCCTGTCAATGTGGACAAGATTGAGTTCGTTGACCACGACGCCAA
GTTCGTCATCGCCATTGAAACCGGCGGTATGAGGGACAGGCTTGTGGAGAACGGATTTGATGAGAAGTACGATGCAATCA
TAGTCCATCTGAAGGGCCAGCCTGCGAGAAGCACCAGGAGGCTACTCAGGAGGCTCAACACTGAGCTAAATTTGCCGGTT
GTTGTCTTCACCGATGGCGACCCCTGGAGCTACAGAATCTTCGCCAGCGTAGCTTACGGAAGCATCAAATCCGCCCACCT
TTCCGAGTATCTCGCCACCCCAGCTGCGCAGTTTGTCGGAATAAGGCCGACGGACATCGTCAAGTATGACCTGCCAGCTG
ACAAGCTTACGGAGGAAGACATCAAAGCTCTCAACGCCATTCTTACCGACCCACGCTTTGACTCCGAGTTCTGGAAGAAG
GAGGTAAACCTGCAGCTCGAGATAAACAAGAAGTCAGAGCAGCAGGCCCTGGCAAAGTACGGTTTGGACTACGTTACCGA
TGTCTATCTTCCGGAGAGACTTTCCGAGCTTGGCGTGATTTAG

Upstream 100 bases:

>100_bases
TCTAATAATCTCTCCCATTCTGTCAAGAAACGAATTGATGTAGAGCATCAGATTGAGTTATTGGTAAGGGCTATAAATCC
GTTGCGATAATGCATTTTTA

Downstream 100 bases:

>100_bases
CTCTCATCGAAGGTGACCTCAAGGCTCATAAAATCCTCAGCGATAATCGTGTTCTCAAACACCTTCTTTGCCTCTTCAAG
CAGTGGAGAGGCGTCTTTGG

Product: DNA topoisomerase VI subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKEIERRCLRALIGIVQNIYDQMKAGQVPELHIATRTKYNIEFNEESEVWVYGDRKSVRSAKTVKGAYRILKMTYVIGFL
KEQLNLNKSSTLRELYYISEGWGAAKFEEQPESDRLVEDLEILTNFQREHFHIRPEEDGATVIGPLRVREETRRGVREIH
CQDDVGEGGYQIPVNVDKIEFVDHDAKFVIAIETGGMRDRLVENGFDEKYDAIIVHLKGQPARSTRRLLRRLNTELNLPV
VVFTDGDPWSYRIFASVAYGSIKSAHLSEYLATPAAQFVGIRPTDIVKYDLPADKLTEEDIKALNAILTDPRFDSEFWKK
EVNLQLEINKKSEQQALAKYGLDYVTDVYLPERLSELGVI

Sequences:

>Translated_360_residues
MKEIERRCLRALIGIVQNIYDQMKAGQVPELHIATRTKYNIEFNEESEVWVYGDRKSVRSAKTVKGAYRILKMTYVIGFL
KEQLNLNKSSTLRELYYISEGWGAAKFEEQPESDRLVEDLEILTNFQREHFHIRPEEDGATVIGPLRVREETRRGVREIH
CQDDVGEGGYQIPVNVDKIEFVDHDAKFVIAIETGGMRDRLVENGFDEKYDAIIVHLKGQPARSTRRLLRRLNTELNLPV
VVFTDGDPWSYRIFASVAYGSIKSAHLSEYLATPAAQFVGIRPTDIVKYDLPADKLTEEDIKALNAILTDPRFDSEFWKK
EVNLQLEINKKSEQQALAKYGLDYVTDVYLPERLSELGVI
>Mature_360_residues
MKEIERRCLRALIGIVQNIYDQMKAGQVPELHIATRTKYNIEFNEESEVWVYGDRKSVRSAKTVKGAYRILKMTYVIGFL
KEQLNLNKSSTLRELYYISEGWGAAKFEEQPESDRLVEDLEILTNFQREHFHIRPEEDGATVIGPLRVREETRRGVREIH
CQDDVGEGGYQIPVNVDKIEFVDHDAKFVIAIETGGMRDRLVENGFDEKYDAIIVHLKGQPARSTRRLLRRLNTELNLPV
VVFTDGDPWSYRIFASVAYGSIKSAHLSEYLATPAAQFVGIRPTDIVKYDLPADKLTEEDIKALNAILTDPRFDSEFWKK
EVNLQLEINKKSEQQALAKYGLDYVTDVYLPERLSELGVI

Specific function: Relaxes both positive and negative superturns and exhibits a strong decatenase activity

COG id: COG1697

COG function: function code L; DNA topoisomerase VI, subunit A

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TOP6A family

Homologues:

Organism=Homo sapiens, GI6912680, Length=359, Percent_Identity=28.133704735376, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI38201680, Length=309, Percent_Identity=29.126213592233, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45552753, Length=193, Percent_Identity=26.9430051813472, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP6A_ARCFU (O29322)

Other databases:

- EMBL:   AE000782
- PIR:   D69367
- RefSeq:   NP_069773.1
- ProteinModelPortal:   O29322
- SMR:   O29322
- GeneID:   1484163
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0940
- NMPDR:   fig|224325.1.peg.932
- TIGR:   AF_0940
- HOGENOM:   HBG559107
- OMA:   DGDPYGW
- ProtClustDB:   PRK04342
- BioCyc:   AFUL224325:AF_0940-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- HAMAP:   MF_00132
- InterPro:   IPR002815
- InterPro:   IPR013049
- InterPro:   IPR004085
- InterPro:   IPR022904
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10848
- PRINTS:   PR01550
- PRINTS:   PR01552

Pfam domain/function: PF04406 TP6A_N; SSF56726 Spo11/TopoVI_A

EC number: =5.99.1.3

Molecular weight: Translated: 41418; Mature: 41418

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: ACT_SITE 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIERRCLRALIGIVQNIYDQMKAGQVPELHIATRTKYNIEFNEESEVWVYGDRKSVRS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEEEEEECCCCCEEEECCHHHHHH
AKTVKGAYRILKMTYVIGFLKEQLNLNKSSTLRELYYISEGWGAAKFEEQPESDRLVEDL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHH
EILTNFQREHFHIRPEEDGATVIGPLRVREETRRGVREIHCQDDVGEGGYQIPVNVDKIE
HHHHHHHHHHEECCCCCCCCEEEEHHHHHHHHHCCHHEECCCCCCCCCCEEECCCCCEEE
FVDHDAKFVIAIETGGMRDRLVENGFDEKYDAIIVHLKGQPARSTRRLLRRLNTELNLPV
EECCCCEEEEEEECCCCHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCE
VVFTDGDPWSYRIFASVAYGSIKSAHLSEYLATPAAQFVGIRPTDIVKYDLPADKLTEED
EEEECCCCCEEEEEEEEHHCCCHHHHHHHHHHCCHHHHCCCCCCCEEEECCCHHHCCHHH
IKALNAILTDPRFDSEFWKKEVNLQLEINKKSEQQALAKYGLDYVTDVYLPERLSELGVI
HHHHHHHHCCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEIERRCLRALIGIVQNIYDQMKAGQVPELHIATRTKYNIEFNEESEVWVYGDRKSVRS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEEEEEECCCCCEEEECCHHHHHH
AKTVKGAYRILKMTYVIGFLKEQLNLNKSSTLRELYYISEGWGAAKFEEQPESDRLVEDL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHH
EILTNFQREHFHIRPEEDGATVIGPLRVREETRRGVREIHCQDDVGEGGYQIPVNVDKIE
HHHHHHHHHHEECCCCCCCCEEEEHHHHHHHHHCCHHEECCCCCCCCCCEEECCCCCEEE
FVDHDAKFVIAIETGGMRDRLVENGFDEKYDAIIVHLKGQPARSTRRLLRRLNTELNLPV
EECCCCEEEEEEECCCCHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCE
VVFTDGDPWSYRIFASVAYGSIKSAHLSEYLATPAAQFVGIRPTDIVKYDLPADKLTEED
EEEECCCCCEEEEEEEEHHCCCHHHHHHHHHHCCHHHHCCCCCCCEEEECCCHHHCCHHH
IKALNAILTDPRFDSEFWKKEVNLQLEINKKSEQQALAKYGLDYVTDVYLPERLSELGVI
HHHHHHHHCCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475