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The map label for this gene is purF

Identifier: 11498479

GI number: 11498479

Start: 776152

End: 777525

Strand: Reverse

Name: purF

Synonym: AF0873

Alternate gene names: 11498479

Gene position: 777525-776152 (Counterclockwise)

Preceding gene: 11498480

Following gene: 11498477

Centisome position: 35.69

GC content: 52.18

Gene sequence:

>1374_bases
ATGTGTGGGGTTGTAGGTATATACCATCCTGACGGGGAGCTTGCTCCCCGACTCGCCTACTATTCTCTCTTCTCCCTTCA
GCATCGCGGTCAGGAGTCGGCAGGTATCGCGTCCTTTGACAACCACATAAGGCAGAAGAGGGGCATGGGCCTCGTCACCG
AGGTTTTCAACGACGAGGACTTTGAGCTGCTTGCAGGCAAATCGGTAATCGGCCACGTCAGATACTCAACGACCGGTAGG
TCGAGACTTGAGAACGCTCAGCCTTTCGTCGTCAAATCAAAGGCGGGATACATAGCTGTAGCACATAACGGAAACCTCGT
TAACTACTCTCAGCTGAGAAACGAGCTGGAAAACGAGGGAAGGGTTTTCACAACCGATTCAGATACCGAGGTAATTTCCC
AGCTGCTCTCAAAGTTTCTGATCGAGGAAGGGGACATCATAAATGCTCTTGAAAGGCTGAACGAATCACTTGTGGGCAGC
TACACCATGACCATGCTCGTTGACGATGCGGTGATAGGCTACCGCGACCCTCTCGGCTTCAAGCCGCTGTGCGTTGGAAG
GATTGATGATGGTTATGTGATATGCAGCGAAAGCTGCGCAATCGATGCTCTCGGTGGAGAGTTTATAAGAGACGTACAGC
CTGGGAAAGCGGCGATTATAAAAGATGGTGAGCTTGAGTTCGTAAAAATCGCCAAATCGGAGAGGAGAGCTGTTTGCATT
TTCGAGTACATATACTTCGCCCGCCCGGACTCAATAATTGACGGAATAAGCGTTTACAAAGCGAGAAGCGAGATGGGAAA
GGTTCTGGCGAGGGAGTCTCCTGTCGAAGCTGACTTCGTTTCAGCTGTACCCGACAGCGGAATAACCGCGGCAATAGGCT
ACGCTCAGGAATCAGGGCTGCCTTACTTTGAGGGCTTGATAAAGAACAGATACGTGGGCAGGACTTTCATAATGCCGGTT
CAGAGTCTAAGGGAGACGTCAGTGAGGCTGAAGGTAAACGTCGTCAGGGAGAACGTGAGGGGGAGGAGGGTTGTGCTGGT
TGACGACAGCATAGTGAGAGGGACCACCTCAAGGAGAATCGTGCAGATGATAAAAGACGCGGGAGCAAAGGAGGTGCACA
TGAGAATCGGCAGCCCCCCAATCATAGCCCCGTGCTACTTCGGAATAGACATGAAATCGAGGGAGGAGCTGATAGCCGCC
AGCCATACCGTTGAGGAGATAGGGAGAATTTTCGGAACGGATAGCCTCGCCTACCTGAGCCTGGAAGGTCTTCTCGAAGC
GGTTAGGAGGGCGGGTGGAAAGAGGGGATACTGCCTTGCCTGTCTGACCTCCAAATACCCCGTTTCAGTGCCTGGAGAGG
AGTGTGAGTGTTAA

Upstream 100 bases:

>100_bases
ATTCCACAGAAGAAAGGGTTATTGCGCTGCATGTGGGTTCGGAAGGAGCAAGAGGCTTAGAAGCTACAACTGGGTTAGAA
AGAAGAAAAACAGAACGTAA

Downstream 100 bases:

>100_bases
AAAGTTAAATCTTAAAATAGGGCAGCCACATCATCACAAGCAGCAGAATCAGAATTCCGAACATCAGAATCATGAAGTTC
CTCTGCCTCTTCCTCTCTTT

Product: amidophosphoribosyltransferase (purF)

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MCGVVGIYHPDGELAPRLAYYSLFSLQHRGQESAGIASFDNHIRQKRGMGLVTEVFNDEDFELLAGKSVIGHVRYSTTGR
SRLENAQPFVVKSKAGYIAVAHNGNLVNYSQLRNELENEGRVFTTDSDTEVISQLLSKFLIEEGDIINALERLNESLVGS
YTMTMLVDDAVIGYRDPLGFKPLCVGRIDDGYVICSESCAIDALGGEFIRDVQPGKAAIIKDGELEFVKIAKSERRAVCI
FEYIYFARPDSIIDGISVYKARSEMGKVLARESPVEADFVSAVPDSGITAAIGYAQESGLPYFEGLIKNRYVGRTFIMPV
QSLRETSVRLKVNVVRENVRGRRVVLVDDSIVRGTTSRRIVQMIKDAGAKEVHMRIGSPPIIAPCYFGIDMKSREELIAA
SHTVEEIGRIFGTDSLAYLSLEGLLEAVRRAGGKRGYCLACLTSKYPVSVPGEECEC

Sequences:

>Translated_457_residues
MCGVVGIYHPDGELAPRLAYYSLFSLQHRGQESAGIASFDNHIRQKRGMGLVTEVFNDEDFELLAGKSVIGHVRYSTTGR
SRLENAQPFVVKSKAGYIAVAHNGNLVNYSQLRNELENEGRVFTTDSDTEVISQLLSKFLIEEGDIINALERLNESLVGS
YTMTMLVDDAVIGYRDPLGFKPLCVGRIDDGYVICSESCAIDALGGEFIRDVQPGKAAIIKDGELEFVKIAKSERRAVCI
FEYIYFARPDSIIDGISVYKARSEMGKVLARESPVEADFVSAVPDSGITAAIGYAQESGLPYFEGLIKNRYVGRTFIMPV
QSLRETSVRLKVNVVRENVRGRRVVLVDDSIVRGTTSRRIVQMIKDAGAKEVHMRIGSPPIIAPCYFGIDMKSREELIAA
SHTVEEIGRIFGTDSLAYLSLEGLLEAVRRAGGKRGYCLACLTSKYPVSVPGEECEC
>Mature_457_residues
MCGVVGIYHPDGELAPRLAYYSLFSLQHRGQESAGIASFDNHIRQKRGMGLVTEVFNDEDFELLAGKSVIGHVRYSTTGR
SRLENAQPFVVKSKAGYIAVAHNGNLVNYSQLRNELENEGRVFTTDSDTEVISQLLSKFLIEEGDIINALERLNESLVGS
YTMTMLVDDAVIGYRDPLGFKPLCVGRIDDGYVICSESCAIDALGGEFIRDVQPGKAAIIKDGELEFVKIAKSERRAVCI
FEYIYFARPDSIIDGISVYKARSEMGKVLARESPVEADFVSAVPDSGITAAIGYAQESGLPYFEGLIKNRYVGRTFIMPV
QSLRETSVRLKVNVVRENVRGRRVVLVDDSIVRGTTSRRIVQMIKDAGAKEVHMRIGSPPIIAPCYFGIDMKSREELIAA
SHTVEEIGRIFGTDSLAYLSLEGLLEAVRRAGGKRGYCLACLTSKYPVSVPGEECEC

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Homo sapiens, GI29570798, Length=504, Percent_Identity=41.0714285714286, Blast_Score=369, Evalue=1e-102,
Organism=Escherichia coli, GI1788651, Length=453, Percent_Identity=43.4878587196468, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1790167, Length=249, Percent_Identity=30.5220883534137, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=41.7536534446764, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6323958, Length=456, Percent_Identity=40.5701754385965, Blast_Score=295, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24659604, Length=462, Percent_Identity=44.8051948051948, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24659598, Length=462, Percent_Identity=44.8051948051948, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI28573187, Length=465, Percent_Identity=44.7311827956989, Blast_Score=378, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR1_ARCFU (O29388)

Other databases:

- EMBL:   AE000782
- PIR:   A69359
- RefSeq:   NP_069707.1
- ProteinModelPortal:   O29388
- SMR:   O29388
- GeneID:   1484093
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0873
- NMPDR:   fig|224325.1.peg.866
- TIGR:   AF_0873
- HOGENOM:   HBG392416
- OMA:   DSMFEDQ
- ProtClustDB:   CLSK2746637
- BioCyc:   AFUL224325:AF_0873-MONOMER
- BRENDA:   2.4.2.14
- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836
- PANTHER:   PTHR11907
- PIRSF:   PIRSF000485
- TIGRFAMs:   TIGR01134

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran

EC number: =2.4.2.14

Molecular weight: Translated: 50342; Mature: 50342

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS51278 GATASE_TYPE_2; PS00103 PUR_PYR_PR_TRANSFER; PS00443 GATASE_TYPE_II

Important sites: ACT_SITE 2-2

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVVGIYHPDGELAPRLAYYSLFSLQHRGQESAGIASFDNHIRQKRGMGLVTEVFNDED
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCC
FELLAGKSVIGHVRYSTTGRSRLENAQPFVVKSKAGYIAVAHNGNLVNYSQLRNELENEG
CEEEECCCEEEEEEECCCCHHHHCCCCCEEEEECCCEEEEEECCCEEEHHHHHHHHHHCC
RVFTTDSDTEVISQLLSKFLIEEGDIINALERLNESLVGSYTMTMLVDDAVIGYRDPLGF
EEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEEHHHHHCCCCCCCC
KPLCVGRIDDGYVICSESCAIDALGGEFIRDVQPGKAAIIKDGELEFVKIAKSERRAVCI
CCEEEEECCCCEEEECCCCEEECCCHHHHHHCCCCCEEEEECCCEEEEEEECCCCCEEEE
FEYIYFARPDSIIDGISVYKARSEMGKVLARESPVEADFVSAVPDSGITAAIGYAQESGL
EEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECHHHCCC
PYFEGLIKNRYVGRTFIMPVQSLRETSVRLKVNVVRENVRGRRVVLVDDSIVRGTTSRRI
HHHHHHHHCCCCCEEEEHHHHHHHHCCEEEEEEEEECCCCCCEEEEECCCHHCCCHHHHH
VQMIKDAGAKEVHMRIGSPPIIAPCYFGIDMKSREELIAASHTVEEIGRIFGTDSLAYLS
HHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
LEGLLEAVRRAGGKRGYCLACLTSKYPVSVPGEECEC
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGVVGIYHPDGELAPRLAYYSLFSLQHRGQESAGIASFDNHIRQKRGMGLVTEVFNDED
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCC
FELLAGKSVIGHVRYSTTGRSRLENAQPFVVKSKAGYIAVAHNGNLVNYSQLRNELENEG
CEEEECCCEEEEEEECCCCHHHHCCCCCEEEEECCCEEEEEECCCEEEHHHHHHHHHHCC
RVFTTDSDTEVISQLLSKFLIEEGDIINALERLNESLVGSYTMTMLVDDAVIGYRDPLGF
EEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEEHHHHHCCCCCCCC
KPLCVGRIDDGYVICSESCAIDALGGEFIRDVQPGKAAIIKDGELEFVKIAKSERRAVCI
CCEEEEECCCCEEEECCCCEEECCCHHHHHHCCCCCEEEEECCCEEEEEEECCCCCEEEE
FEYIYFARPDSIIDGISVYKARSEMGKVLARESPVEADFVSAVPDSGITAAIGYAQESGL
EEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECHHHCCC
PYFEGLIKNRYVGRTFIMPVQSLRETSVRLKVNVVRENVRGRRVVLVDDSIVRGTTSRRI
HHHHHHHHCCCCCEEEEHHHHHHHHCCEEEEEEEEECCCCCCEEEEECCCHHCCCHHHHH
VQMIKDAGAKEVHMRIGSPPIIAPCYFGIDMKSREELIAASHTVEEIGRIFGTDSLAYLS
HHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
LEGLLEAVRRAGGKRGYCLACLTSKYPVSVPGEECEC
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475