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The map label for this gene is dapF

Identifier: 11498353

GI number: 11498353

Start: 666467

End: 667309

Strand: Reverse

Name: dapF

Synonym: AF0747

Alternate gene names: 11498353

Gene position: 667309-666467 (Counterclockwise)

Preceding gene: 11498358

Following gene: 11498352

Centisome position: 30.63

GC content: 52.91

Gene sequence:

>843_bases
ATGCGAATTGCCTTTACTAAAATGCACGGTAACGGTAACGACTTCGTCCTGATTGACGAATTCGAAGGAGTGATTGTGGG
AGAAGAGGAGAAGCCGAGGTTTGTAAGGGCAGTATGCCACAGAAACTTCGGCGTTGGTGCTGACGGTGCGCTTTTTGTTC
AGCCCTCGCAAAAGGCGGACGTCAGGTTCAGGTACTTCAACAGCGATGGCAGCGAGGCTGCGATGTGCGGGAACGGTATA
AGGTGCTTCTCCCGCTACGTCGTGGAGGAGGGCTATGCTGGGGAGAGGTTGAGAGTAGAAACCCTCGCTGGGATTCTTGA
GCTTGAGGTAAAGAGGGAGAACGGCTGGTGGGTGAAGGTTGACATGGGAAAGCCTAAGTTTGGAAGGGAGGAGATTCCAG
CAAAAACCGACGTGTGGGGGTATGAGGTGGAGCACGACGGCAGGAAGTTCAGGATTTACGCTGCCAACACCGGAGTGCCG
CACGTGGCCGTTTTCGTGGACAGTCTCGACTTCGACATCGTCCCCCTTGCGAGAAAAATCCGCTATTCCGAGATTTTTCC
GGAGGGAACCAACGTAAACTTTGCGAAAGTTGATGGGGACACAATTACGGTCAGAACCTACGAGAGGGGTGTTGAAGGGG
AAACACTGAGCTGCGGAACCGGAAGCGTTGCCGTTGCGGCAATAGCAAACAGGCTCGGCCTAACCGGAAGTAAGGTTGAC
GTTGTAACGAAGGGAGGTTTGCTCAAGATAGAGCTTACAGAGGATACTGCCTACATGACGGGTGGGGCGAGCAGAGTTTT
CGACGGGATTTTGAGGCTAAACGAGCTGAGATATGATATTTGA

Upstream 100 bases:

>100_bases
AAGTCATCAAACCCTCCGCCGGCGTGCGGATTCAGAACGATGCCGACCTTCACAGATAAAGTTGCCGGGAACTTTTAAAA
ATCTCGCCCGAACTTGCTTC

Downstream 100 bases:

>100_bases
GAACCGCCTCGCTCTCTCTGCGATGGCCGGAGTGAACAATGCGGAGTTTTGCAGAAAGCAGAAAGCGTCGCTTGTAATCC
TAGGAGGGTTCAATGCTGAC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MRIAFTKMHGNGNDFVLIDEFEGVIVGEEEKPRFVRAVCHRNFGVGADGALFVQPSQKADVRFRYFNSDGSEAAMCGNGI
RCFSRYVVEEGYAGERLRVETLAGILELEVKRENGWWVKVDMGKPKFGREEIPAKTDVWGYEVEHDGRKFRIYAANTGVP
HVAVFVDSLDFDIVPLARKIRYSEIFPEGTNVNFAKVDGDTITVRTYERGVEGETLSCGTGSVAVAAIANRLGLTGSKVD
VVTKGGLLKIELTEDTAYMTGGASRVFDGILRLNELRYDI

Sequences:

>Translated_280_residues
MRIAFTKMHGNGNDFVLIDEFEGVIVGEEEKPRFVRAVCHRNFGVGADGALFVQPSQKADVRFRYFNSDGSEAAMCGNGI
RCFSRYVVEEGYAGERLRVETLAGILELEVKRENGWWVKVDMGKPKFGREEIPAKTDVWGYEVEHDGRKFRIYAANTGVP
HVAVFVDSLDFDIVPLARKIRYSEIFPEGTNVNFAKVDGDTITVRTYERGVEGETLSCGTGSVAVAAIANRLGLTGSKVD
VVTKGGLLKIELTEDTAYMTGGASRVFDGILRLNELRYDI
>Mature_280_residues
MRIAFTKMHGNGNDFVLIDEFEGVIVGEEEKPRFVRAVCHRNFGVGADGALFVQPSQKADVRFRYFNSDGSEAAMCGNGI
RCFSRYVVEEGYAGERLRVETLAGILELEVKRENGWWVKVDMGKPKFGREEIPAKTDVWGYEVEHDGRKFRIYAANTGVP
HVAVFVDSLDFDIVPLARKIRYSEIFPEGTNVNFAKVDGDTITVRTYERGVEGETLSCGTGSVAVAAIANRLGLTGSKVD
VVTKGGLLKIELTEDTAYMTGGASRVFDGILRLNELRYDI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=280, Percent_Identity=36.0714285714286, Blast_Score=148, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_ARCFU (O29511)

Other databases:

- EMBL:   AE000782
- PIR:   C69343
- RefSeq:   NP_069581.1
- ProteinModelPortal:   O29511
- SMR:   O29511
- GeneID:   1483964
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0747
- NMPDR:   fig|224325.1.peg.740
- TIGR:   AF_0747
- HOGENOM:   HBG399442
- OMA:   NACGTAM
- ProtClustDB:   PRK00450
- BioCyc:   AFUL224325:AF_0747-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31024; Mature: 31024

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 76-76 ACT_SITE 218-218

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAFTKMHGNGNDFVLIDEFEGVIVGEEEKPRFVRAVCHRNFGVGADGALFVQPSQKAD
CEEEEEEEECCCCCEEEEECCCCEEECCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCC
VRFRYFNSDGSEAAMCGNGIRCFSRYVVEEGYAGERLRVETLAGILELEVKRENGWWVKV
EEEEEECCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEHHHHHCEEEEEEEECCCCEEEE
DMGKPKFGREEIPAKTDVWGYEVEHDGRKFRIYAANTGVPHVAVFVDSLDFDIVPLARKI
ECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCCCEEHHHHHH
RYSEIFPEGTNVNFAKVDGDTITVRTYERGVEGETLSCGTGSVAVAAIANRLGLTGSKVD
HHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHCCCCCEEE
VVTKGGLLKIELTEDTAYMTGGASRVFDGILRLNELRYDI
EEECCCEEEEEEECCCEEEECCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRIAFTKMHGNGNDFVLIDEFEGVIVGEEEKPRFVRAVCHRNFGVGADGALFVQPSQKAD
CEEEEEEEECCCCCEEEEECCCCEEECCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCC
VRFRYFNSDGSEAAMCGNGIRCFSRYVVEEGYAGERLRVETLAGILELEVKRENGWWVKV
EEEEEECCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEHHHHHCEEEEEEEECCCCEEEE
DMGKPKFGREEIPAKTDVWGYEVEHDGRKFRIYAANTGVPHVAVFVDSLDFDIVPLARKI
ECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCCCEEHHHHHH
RYSEIFPEGTNVNFAKVDGDTITVRTYERGVEGETLSCGTGSVAVAAIANRLGLTGSKVD
HHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHCCCCCEEE
VVTKGGLLKIELTEDTAYMTGGASRVFDGILRLNELRYDI
EEECCCEEEEEEECCCEEEECCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475