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The map label for this gene is thiL

Identifier: 11498340

GI number: 11498340

Start: 656138

End: 657028

Strand: Reverse

Name: thiL

Synonym: AF0733

Alternate gene names: 11498340

Gene position: 657028-656138 (Counterclockwise)

Preceding gene: 11498341

Following gene: 11498334

Centisome position: 30.16

GC content: 52.19

Gene sequence:

>891_bases
GTGTTCTATGCGAATATGAAAGAGTTCGAGCTCATAAAGTTGGTTGAGCGCCTGTTTGGGGATGAAGATGTCATTACTCC
CGCAGGAAAGCACGATGCTGCTTACGTCAAAATCGGAAACAGGCTGATCGTCCTCACCTGTGATACCGTCAACGAAAAAA
GCGATTTTCCACCTTACATGCTTCCCGAAGAAATGGGGTGGATGGCGATTGCTGTCACCCTCTCGGACGTAGCTGCCTGC
GGAGCAAGACCTCTTTACTTCCTCAGCTCAATCTCGCTCAAATCGATCGAGTTCTTCGAGGACATACTGCTCGGAATCAA
GAGGTGTGCGGACAGATTCGGAGTGAAGGTTGTCGGGGGGGATATCGATTTCAGCGAGATAACGACGATAGCCGGTTTTG
CCATCGGCGAAGCGAGAAGGCACATAACGAGGAGGGGGGCGAAGGTGGGAGAGGGGGTTTTCATAACCGACCTTCCGGGA
AAAGCTCAGCTCTGCCTGGAAATGCTCGAAAGAGGGGCGAAGAGGGAGGAGCTGCCTTATGCGGAAAAGCTCTACACTCC
CGTGCCGAGGATTGAGGAGGGAATGAGAATTGCCAGATACGCCTCGGCCATGACCGACGTGAGCGACAGCCTTGCCGTCA
GCCTCCATCAGATCAGCCAGAGCAGCAACGTGAAGATAGTTCTCGACAACATAGTTTTATCTCATCTCAGCCCAGCGGAG
AATGCACTGGAGCTCTTTCTTTACGGTGGAGGTGATTTTGAGCTGGTTTACACCGCAAAAAGCAGCGATGATGGCATCAG
AATTGGCAGAGTTGAGCGGGGCAAGGGCGTTTTTGCAGAGATTGACGGGAAGACCTTTGAGGTGGAGTTCAGAGGTTACT
CCCACTTTTAG

Upstream 100 bases:

>100_bases
CCGCACGGTGACTTTTTCGAGGAAACCCTTCGGGAGCTTGGGGAGTTTAAGGAGGTAAGTCTTGGTACAGAAAGCTACAC
AAGCCTTTATGTGACGAGTC

Downstream 100 bases:

>100_bases
CGCTCTCCAAAAACAACAAAAACAGGATTTAGCGAGCGAAAAGCCAAACCACCAGCCAGCTCGTAGCTTTTCGCCGCATT
AACTAAAACAATTTCTCTGA

Product: thiamine monophosphate kinase (thiL)

Products: NA

Alternate protein names: Thiamine-phosphate kinase

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MFYANMKEFELIKLVERLFGDEDVITPAGKHDAAYVKIGNRLIVLTCDTVNEKSDFPPYMLPEEMGWMAIAVTLSDVAAC
GARPLYFLSSISLKSIEFFEDILLGIKRCADRFGVKVVGGDIDFSEITTIAGFAIGEARRHITRRGAKVGEGVFITDLPG
KAQLCLEMLERGAKREELPYAEKLYTPVPRIEEGMRIARYASAMTDVSDSLAVSLHQISQSSNVKIVLDNIVLSHLSPAE
NALELFLYGGGDFELVYTAKSSDDGIRIGRVERGKGVFAEIDGKTFEVEFRGYSHF

Sequences:

>Translated_296_residues
MFYANMKEFELIKLVERLFGDEDVITPAGKHDAAYVKIGNRLIVLTCDTVNEKSDFPPYMLPEEMGWMAIAVTLSDVAAC
GARPLYFLSSISLKSIEFFEDILLGIKRCADRFGVKVVGGDIDFSEITTIAGFAIGEARRHITRRGAKVGEGVFITDLPG
KAQLCLEMLERGAKREELPYAEKLYTPVPRIEEGMRIARYASAMTDVSDSLAVSLHQISQSSNVKIVLDNIVLSHLSPAE
NALELFLYGGGDFELVYTAKSSDDGIRIGRVERGKGVFAEIDGKTFEVEFRGYSHF
>Mature_296_residues
MFYANMKEFELIKLVERLFGDEDVITPAGKHDAAYVKIGNRLIVLTCDTVNEKSDFPPYMLPEEMGWMAIAVTLSDVAAC
GARPLYFLSSISLKSIEFFEDILLGIKRCADRFGVKVVGGDIDFSEITTIAGFAIGEARRHITRRGAKVGEGVFITDLPG
KAQLCLEMLERGAKREELPYAEKLYTPVPRIEEGMRIARYASAMTDVSDSLAVSLHQISQSSNVKIVLDNIVLSHLSPAE
NALELFLYGGGDFELVYTAKSSDDGIRIGRVERGKGVFAEIDGKTFEVEFRGYSHF

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family

Homologues:

Organism=Escherichia coli, GI1786619, Length=299, Percent_Identity=26.7558528428094, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIL_ARCFU (O29525)

Other databases:

- EMBL:   AE000782
- PIR:   E69341
- RefSeq:   NP_069567.1
- ProteinModelPortal:   O29525
- GeneID:   1483951
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0733
- NMPDR:   fig|224325.1.peg.726
- TIGR:   AF_0733
- HOGENOM:   HBG577902
- OMA:   CLEMLER
- ProtClustDB:   CLSK2746567
- BioCyc:   AFUL224325:AF_0733-MONOMER
- BRENDA:   2.7.4.16
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283
- PIRSF:   PIRSF005303
- TIGRFAMs:   TIGR01379

Pfam domain/function: PF00586 AIRS; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: =2.7.4.16

Molecular weight: Translated: 32784; Mature: 32784

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYANMKEFELIKLVERLFGDEDVITPAGKHDAAYVKIGNRLIVLTCDTVNEKSDFPPYM
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCC
LPEEMGWMAIAVTLSDVAACGARPLYFLSSISLKSIEFFEDILLGIKRCADRFGVKVVGG
CCCCCCCEEEEEEHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEEC
DIDFSEITTIAGFAIGEARRHITRRGAKVGEGVFITDLPGKAQLCLEMLERGAKREELPY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCC
AEKLYTPVPRIEEGMRIARYASAMTDVSDSLAVSLHQISQSSNVKIVLDNIVLSHLSPAE
HHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCC
NALELFLYGGGDFELVYTAKSSDDGIRIGRVERGKGVFAEIDGKTFEVEFRGYSHF
CEEEEEEECCCCEEEEEEECCCCCCEEEEEEECCCCEEEEECCEEEEEEECCCCCC
>Mature Secondary Structure
MFYANMKEFELIKLVERLFGDEDVITPAGKHDAAYVKIGNRLIVLTCDTVNEKSDFPPYM
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCC
LPEEMGWMAIAVTLSDVAACGARPLYFLSSISLKSIEFFEDILLGIKRCADRFGVKVVGG
CCCCCCCEEEEEEHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEEC
DIDFSEITTIAGFAIGEARRHITRRGAKVGEGVFITDLPGKAQLCLEMLERGAKREELPY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCC
AEKLYTPVPRIEEGMRIARYASAMTDVSDSLAVSLHQISQSSNVKIVLDNIVLSHLSPAE
HHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCC
NALELFLYGGGDFELVYTAKSSDDGIRIGRVERGKGVFAEIDGKTFEVEFRGYSHF
CEEEEEEECCCCEEEEEEECCCCCCEEEEEEECCCCEEEEECCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475