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The map label for this gene is cbiF

Identifier: 11498333

GI number: 11498333

Start: 651749

End: 652453

Strand: Reverse

Name: cbiF

Synonym: AF0726

Alternate gene names: 11498333

Gene position: 652453-651749 (Counterclockwise)

Preceding gene: 11498334

Following gene: 11498332

Centisome position: 29.95

GC content: 49.93

Gene sequence:

>705_bases
ATGAAGGTTTACTTCGTGGGCTTCGGACCGGGAAATCCAGAGCTTTTAACCTTAAAGGCCTACAAACTGCTTAAGGAGGC
GGATTTGGTAGTTTACCCCGGCTCGATTGTTCCTGAGAAGTTTTTTGAGGAGTTTAAGGGGGAGAAGGTGAACAGCCACG
GCAAAACCCTCGAAGAGATTGTTGATTTGATTGAAAGATACGTGAAAAGGGGAAAGAGGGTTGTTAGGGTGCAGAGTGGA
GATCCATCAATTTTCGGGGCTGTCTGGGAGCAAATAAGGGAGCTGAGGAGGAGAGGGATTGACTGCGAGGTTGTTCCGGG
TGTGAGCAGCGTTTTTGCTTCAGCCGCATCACTTGGAATTGAGCTGACCTCCCCCTCAACGGAGGGAGTTGCTATAGTCA
GGCCAGCAGGGAAAACTCTTGAGAAAGACCATCTTGAGGAGCTTGCAAGGCTGCCCCTCACTCTCGTAATTCTCCTTGGC
GTTGACAAAATTGGTGAGATAGCGGAAAAAATTGCAAGAGTGAGGGGGTGGGATGAGCCCTGCGCCGTTGTCTTCCACGC
CTCAAGGGAGGATGAGATAAGGATTAAGGGCACACTCAAAGATATTGCGGAGAAGGTGAGGGAGAAGGGGATAAGAAGGA
CTGCAACCATTATTGTGGGAAAGGTCGTTGAGGGATACGATGAGAGGTCGCACCTCTACTCTTAA

Upstream 100 bases:

>100_bases
TGCTTGAAAAGCTTTTCATGAATGGGGAGAAAATTTACTGTGAAATGCCCGAGAAGTCGAGCTACTTCAGCGTGATGGTT
GCGAGGAGAGTTGAGGAGAG

Downstream 100 bases:

>100_bases
GGTTGCCATTCTCTGCTTTGAAAAGGACAGGCCAAAGCTTGAAGCCCTGAAGGAAATTCTCGGCAGAAGCTACGAGGTGG
AGTTTGTTGATTACAGCAAG

Product: cobalamin biosynthesis precorrin-3 methylase (cbiF)

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKVYFVGFGPGNPELLTLKAYKLLKEADLVVYPGSIVPEKFFEEFKGEKVNSHGKTLEEIVDLIERYVKRGKRVVRVQSG
DPSIFGAVWEQIRELRRRGIDCEVVPGVSSVFASAASLGIELTSPSTEGVAIVRPAGKTLEKDHLEELARLPLTLVILLG
VDKIGEIAEKIARVRGWDEPCAVVFHASREDEIRIKGTLKDIAEKVREKGIRRTATIIVGKVVEGYDERSHLYS

Sequences:

>Translated_234_residues
MKVYFVGFGPGNPELLTLKAYKLLKEADLVVYPGSIVPEKFFEEFKGEKVNSHGKTLEEIVDLIERYVKRGKRVVRVQSG
DPSIFGAVWEQIRELRRRGIDCEVVPGVSSVFASAASLGIELTSPSTEGVAIVRPAGKTLEKDHLEELARLPLTLVILLG
VDKIGEIAEKIARVRGWDEPCAVVFHASREDEIRIKGTLKDIAEKVREKGIRRTATIIVGKVVEGYDERSHLYS
>Mature_234_residues
MKVYFVGFGPGNPELLTLKAYKLLKEADLVVYPGSIVPEKFFEEFKGEKVNSHGKTLEEIVDLIERYVKRGKRVVRVQSG
DPSIFGAVWEQIRELRRRGIDCEVVPGVSSVFASAASLGIELTSPSTEGVAIVRPAGKTLEKDHLEELARLPLTLVILLG
VDKIGEIAEKIARVRGWDEPCAVVFHASREDEIRIKGTLKDIAEKVREKGIRRTATIIVGKVVEGYDERSHLYS

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=242, Percent_Identity=31.8181818181818, Blast_Score=97, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6322922, Length=239, Percent_Identity=27.1966527196653, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 26040; Mature: 26040

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYFVGFGPGNPELLTLKAYKLLKEADLVVYPGSIVPEKFFEEFKGEKVNSHGKTLEEI
CEEEEEEECCCCCCEEEHHHHHHHHHCCEEEECCCCCCHHHHHHHCCCCCCCCCCHHHHH
VDLIERYVKRGKRVVRVQSGDPSIFGAVWEQIRELRRRGIDCEVVPGVSSVFASAASLGI
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCE
ELTSPSTEGVAIVRPAGKTLEKDHLEELARLPLTLVILLGVDKIGEIAEKIARVRGWDEP
EEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCC
CAVVFHASREDEIRIKGTLKDIAEKVREKGIRRTATIIVGKVVEGYDERSHLYS
EEEEEECCCCCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure
MKVYFVGFGPGNPELLTLKAYKLLKEADLVVYPGSIVPEKFFEEFKGEKVNSHGKTLEEI
CEEEEEEECCCCCCEEEHHHHHHHHHCCEEEECCCCCCHHHHHHHCCCCCCCCCCHHHHH
VDLIERYVKRGKRVVRVQSGDPSIFGAVWEQIRELRRRGIDCEVVPGVSSVFASAASLGI
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCE
ELTSPSTEGVAIVRPAGKTLEKDHLEELARLPLTLVILLGVDKIGEIAEKIARVRGWDEP
EEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCC
CAVVFHASREDEIRIKGTLKDIAEKVREKGIRRTATIIVGKVVEGYDERSHLYS
EEEEEECCCCCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]