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The map label for this gene is cbiH

Identifier: 11498331

GI number: 11498331

Start: 649737

End: 651077

Strand: Reverse

Name: cbiH

Synonym: AF0724

Alternate gene names: 11498331

Gene position: 651077-649737 (Counterclockwise)

Preceding gene: 11498332

Following gene: 11498330

Centisome position: 29.89

GC content: 51.53

Gene sequence:

>1341_bases
GTGTTACTGTTGCCATCGCGTGGTAAGCTCTACGTGGTTGGAATAGGCCCGGGAAAGGAGGAGCTGATGACATTGAAGGC
AAAAAGGGCCATTGAGGAGGCTGATTATATCGTAGGCTATCAGACCTACGTTGATAGAATCTCACATCTAATTGAGGGGA
AGAAGGTCGTCACGACTCCAATGAGGAAGGAGTTGGATAGAGTAAAGATTGCCCTTGAGCTTGCCAAAGAGCACGTTGTT
GCCCTCATCAGCGGTGGTGATCCATCCATTTATGGCATCCTTCCTCTCGTCATTGAGTATGCTGTAGAGAAAAAGGTTGA
TGTTGAAATTGAGGCTATTCCGGGTGTAACAGCTGCCTCAGCAGCCTCATCACTGCTCGGCTCAGCTATAAGCGGTGATT
TTGCTGTTGTTAGCCTCAGCGATTTGCTGGTTCCGTGGAGTGTGGTGGAGAAGAGGCTCCTTTACGCCCTAAGCGGGGAT
TTTGTGGTGGCAATCTACAACCCATCCAGCAGAAGGAGGAAGGAAAACTTCAGAAAGGCAATGGAGATAGTTAGAAGGTT
CAGGGGAGATGCGTGGGTCGGGGTTGTGAGGAACGCGGGCAGAGAGGGGCAGCAGGTTGAAATCAGGAGAGTTTCTGAGG
TTGATGAGGTGGACATGAACACCATTCTGATTGTGGGAAATTCAGAGACGAAGGTTGTGGATGGGAAGATGTTCACGCCC
CGCGGTTACTCGAATAAGTACAATATCGGTGAAAAGAGAAGGGCGGAAAGGATGGGAGCATCGACGAAAGGTGGAATGGA
GGTGGCGAGAAGGAGCGAGGAGATTCTAAGGAGCTTTTATCCTGAAGAGGGGCTGAGAGGGGACATAATTAGGAGATGCA
TCGCGACAACGGGGGATGTGACGATAAAGGACGTTATCAGGTTTGTGGGGGATACCGAGGAGGGAGTCCGAGCTTTGCGG
GATGGCTGCAGGATAATCGCAGACGTGCACATGGTAAGGGCCGGTTTGAGGAGAGATGCGATTGTTGCTGTAGACTTTGC
CAGAGGTGATGACACGAGGACTGCTTCAGGCATCAGAAATCTTGCGGAGATGATTGAGGGAAGTTTGGTTGCCATTGGCA
ACTCGCCCTCGGCAGCCTTTGCTTTATGCGAGGTGGCTGAGAAGCATCCGCCAAGGTTCATCGTTGCCACTCCCGTTGGC
TTCGTGAATGCTGCAGAGTCGAAGGAGATGGTCAGAAAGCTGCCAGTTCCTTCAGTGACAACCGAGGGGCCGAGAGGAGG
AAGCGGGATTTGTGCTGCGATAGTTAACTGCCTGATCGAGCATGCTGATAGACCCGATTGA

Upstream 100 bases:

>100_bases
ACGAAGGCAGTTAAGATAGGTGTTAAAAATGTGGCCGAAGCCTGTGCAATTTACGCATCAAATTCGGGAGAGCTAATACT
CCCAAAGAGGGTTTACGGAG

Downstream 100 bases:

>100_bases
GCTTTACCGCTATCCCGAAAAGTGGATTAAGGATAGGGATGCGGAGAAGAAGGTAAGGTCAGGGCTTTACATTCTCACGG
AGGACGGCTATCTGCGGAGG

Product: cobalamin biosynthesis precorrin-3 methylase (cbiH)

Products: hydrogenobyrinate

Alternate protein names: Cobalt-precorrin-3B C(17)-methyltransferase; Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase; Cobalt-precorrin-8X methylmutase; Cobalt-precorrin isomerase

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MLLLPSRGKLYVVGIGPGKEELMTLKAKRAIEEADYIVGYQTYVDRISHLIEGKKVVTTPMRKELDRVKIALELAKEHVV
ALISGGDPSIYGILPLVIEYAVEKKVDVEIEAIPGVTAASAASSLLGSAISGDFAVVSLSDLLVPWSVVEKRLLYALSGD
FVVAIYNPSSRRRKENFRKAMEIVRRFRGDAWVGVVRNAGREGQQVEIRRVSEVDEVDMNTILIVGNSETKVVDGKMFTP
RGYSNKYNIGEKRRAERMGASTKGGMEVARRSEEILRSFYPEEGLRGDIIRRCIATTGDVTIKDVIRFVGDTEEGVRALR
DGCRIIADVHMVRAGLRRDAIVAVDFARGDDTRTASGIRNLAEMIEGSLVAIGNSPSAAFALCEVAEKHPPRFIVATPVG
FVNAAESKEMVRKLPVPSVTTEGPRGGSGICAAIVNCLIEHADRPD

Sequences:

>Translated_446_residues
MLLLPSRGKLYVVGIGPGKEELMTLKAKRAIEEADYIVGYQTYVDRISHLIEGKKVVTTPMRKELDRVKIALELAKEHVV
ALISGGDPSIYGILPLVIEYAVEKKVDVEIEAIPGVTAASAASSLLGSAISGDFAVVSLSDLLVPWSVVEKRLLYALSGD
FVVAIYNPSSRRRKENFRKAMEIVRRFRGDAWVGVVRNAGREGQQVEIRRVSEVDEVDMNTILIVGNSETKVVDGKMFTP
RGYSNKYNIGEKRRAERMGASTKGGMEVARRSEEILRSFYPEEGLRGDIIRRCIATTGDVTIKDVIRFVGDTEEGVRALR
DGCRIIADVHMVRAGLRRDAIVAVDFARGDDTRTASGIRNLAEMIEGSLVAIGNSPSAAFALCEVAEKHPPRFIVATPVG
FVNAAESKEMVRKLPVPSVTTEGPRGGSGICAAIVNCLIEHADRPD
>Mature_446_residues
MLLLPSRGKLYVVGIGPGKEELMTLKAKRAIEEADYIVGYQTYVDRISHLIEGKKVVTTPMRKELDRVKIALELAKEHVV
ALISGGDPSIYGILPLVIEYAVEKKVDVEIEAIPGVTAASAASSLLGSAISGDFAVVSLSDLLVPWSVVEKRLLYALSGD
FVVAIYNPSSRRRKENFRKAMEIVRRFRGDAWVGVVRNAGREGQQVEIRRVSEVDEVDMNTILIVGNSETKVVDGKMFTP
RGYSNKYNIGEKRRAERMGASTKGGMEVARRSEEILRSFYPEEGLRGDIIRRCIATTGDVTIKDVIRFVGDTEEGVRALR
DGCRIIADVHMVRAGLRRDAIVAVDFARGDDTRTASGIRNLAEMIEGSLVAIGNSPSAAFALCEVAEKHPPRFIVATPVG
FVNAAESKEMVRKLPVPSVTTEGPRGGSGICAAIVNCLIEHADRPD

Specific function: Bifunctional enzyme with a methyltransferase domain that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 and an isomerase domain that catalyzes the conversion of cobalt-precorrin-8X to cobyrinic acid; a methyl migration

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the CobH family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBIH_ARCFU (O29534)

Other databases:

- EMBL:   AE000782
- PIR:   D69340
- RefSeq:   NP_069558.1
- ProteinModelPortal:   O29534
- GeneID:   1483942
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0724
- NMPDR:   fig|224325.1.peg.717
- TIGR:   AF_0724
- HOGENOM:   HBG321702
- OMA:   ITRTQAG
- ProtClustDB:   CLSK782592
- BioCyc:   AFUL224325:AF_0724-MONOMER
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014422
- InterPro:   IPR003722
- InterPro:   IPR006363
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.40.50.10230
- PIRSF:   PIRSF004874
- TIGRFAMs:   TIGR01466

Pfam domain/function: PF02570 CbiC; PF00590 TP_methylase; SSF63965 CbiC; SSF53790 Cor/por_Metransf

EC number: 5.4.1.2; 2.1.1.131

Molecular weight: Translated: 48679; Mature: 48679

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLPSRGKLYVVGIGPGKEELMTLKAKRAIEEADYIVGYQTYVDRISHLIEGKKVVTTP
CEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCEEEECH
MRKELDRVKIALELAKEHVVALISGGDPSIYGILPLVIEYAVEKKVDVEIEAIPGVTAAS
HHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHH
AASSLLGSAISGDFAVVSLSDLLVPWSVVEKRLLYALSGDFVVAIYNPSSRRRKENFRKA
HHHHHHHHHHCCCEEEEEHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
MEIVRRFRGDAWVGVVRNAGREGQQVEIRRVSEVDEVDMNTILIVGNSETKVVDGKMFTP
HHHHHHHCCCCEEHHHHHCCCCCCEEHHHHHHCCCCCCCCEEEEEECCCCEEECCEEECC
RGYSNKYNIGEKRRAERMGASTKGGMEVARRSEEILRSFYPEEGLRGDIIRRCIATTGDV
CCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCE
TIKDVIRFVGDTEEGVRALRDGCRIIADVHMVRAGLRRDAIVAVDFARGDDTRTASGIRN
EHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHH
LAEMIEGSLVAIGNSPSAAFALCEVAEKHPPRFIVATPVGFVNAAESKEMVRKLPVPSVT
HHHHHCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCHHCCHHHHHHHHHCCCCCCC
TEGPRGGSGICAAIVNCLIEHADRPD
CCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLLLPSRGKLYVVGIGPGKEELMTLKAKRAIEEADYIVGYQTYVDRISHLIEGKKVVTTP
CEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCEEEECH
MRKELDRVKIALELAKEHVVALISGGDPSIYGILPLVIEYAVEKKVDVEIEAIPGVTAAS
HHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHH
AASSLLGSAISGDFAVVSLSDLLVPWSVVEKRLLYALSGDFVVAIYNPSSRRRKENFRKA
HHHHHHHHHHCCCEEEEEHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
MEIVRRFRGDAWVGVVRNAGREGQQVEIRRVSEVDEVDMNTILIVGNSETKVVDGKMFTP
HHHHHHHCCCCEEHHHHHCCCCCCEEHHHHHHCCCCCCCCEEEEEECCCCEEECCEEECC
RGYSNKYNIGEKRRAERMGASTKGGMEVARRSEEILRSFYPEEGLRGDIIRRCIATTGDV
CCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCE
TIKDVIRFVGDTEEGVRALRDGCRIIADVHMVRAGLRRDAIVAVDFARGDDTRTASGIRN
EHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHH
LAEMIEGSLVAIGNSPSAAFALCEVAEKHPPRFIVATPVGFVNAAESKEMVRKLPVPSVT
HHHHHCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCHHCCHHHHHHHHHCCCCCCC
TEGPRGGSGICAAIVNCLIEHADRPD
CCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 8X

Specific reaction: precorrin-8X = hydrogenobyrinate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475