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The map label for this gene is tgtA

Identifier: 11498196

GI number: 11498196

Start: 529954

End: 531399

Strand: Reverse

Name: tgtA

Synonym: AF0588

Alternate gene names: 11498196

Gene position: 531399-529954 (Counterclockwise)

Preceding gene: 11498199

Following gene: 11498195

Centisome position: 24.39

GC content: 52.63

Gene sequence:

>1446_bases
ATGCAGAGGTTCGAGATTCTCGATAAGGATGCAATGGGCAGAATTTGCAGGATTGAGACCCCCCACGGCAGGATAGAGAC
TCCCACGATTCTGCCCGTAATAAATCCAAACATTCCCTTCATCAGAGCTGAGGAGATGAAAAAGTTCGGTGCGCAGGCCG
TGATTACGAACTCCTACATCATCTACAGGAGCATGAGGGAGGAGGCTCTGGAAAAGGGAGTGCACGGAATTCTTGAGACA
GACATGCCCGTAATGACGGACAGCGGGAGCTACCAGCTCATGGTTTACGGCGATGTTGAGATTAAAAATGCAGAGATTGT
GGAGTTCCAGAGGCATATAGGCTCGGACATCATCGTCCCGCTCGACATTCCCACACCGCCCGATGCTGATTATGCTACCG
CAGAATCTGATCTGAGAATCACCCTTGAGAGGGAGAGAGAGGCTAAGGAGCTTTTGAAGGGGGCAGAGAACCTTCTCGCT
GTTCCTGTTCAGGGCTCCACCCACCCCGACCTTAGGAGGTTCGCTGCAGGCGAGGCAAGGAAGATTGGTGGGGACATCTA
CCCCATTGGTGCAGTGGTTCCGCTGATGGATGCTTATCGCTTCAGAGATCTGGCGAGGGTGATTCTTGAGGTCAGGAGTG
CTCTTCCAGTTGAGCCCATCCACCTCTTTGGGTGTGGTCATCCAATGCTCTTTGCCATGGCCGTTGCTCTTGGCTGCGAC
CTCTTTGACTCCGCTGCCTACGCTCTCTACGCCAAGGACGACCGCTACCTTACGGTTTATGGCACAAAGAAGCTGTCGGA
GCTGAACTACTTCCCCTGCAAGTGCCCCGTCTGCTCCAATCACGACCCCGAGGAGCTCAGGAGAATGGAGAAGAATGAGA
GGGAGAGGCTGATTGCGGAGCACAACCTCTACGTCAGCTTTCAGGAGATTGAGACGATAAAGCAGGCTATAAAGGAGAAC
TCTCTGTTCGAGCTTGTTGAGAAGAGGGTGAGAGCGCATCCCAACATGCTGGCTGGATGGAGGCAGGTTAAGCATTACTG
GGAGCTGCTTGAAAAGGCTGACCCCAAAATGAAGAGGAAGTTCCTCTACACCGGCATCGACAGCCTTTACCGCCCTGCAG
TTAGGAGGCATGTCAAGGCAATCAAAAACGTGGAGCTTCCTGAAGAAGTTCTGGTTTCAACAGATTTTGGTATCTACGCC
AACATCTATCTCCGCCCCGTCTTTGGCCCCGTGCCTGCAGAGATGCTGGAAACCTACCCCGCTGGCCATGCAGAAATTCC
TGAAGAGGACGTTGTGGAGGAGGAGGCTCTGAAAGCGGCATCTGAAGCGCTGATGGAGCTTATGAACTCTCATCCAGAGA
AAAGGTTTAAGGTTTACGTGTCAAAGGTGTGGATGAAGCACCTTCAGAATCTCCCACCGAACGGTGAGCTGAATGTTCTA
TCCTGA

Upstream 100 bases:

>100_bases
TTGTCCCGCCCACCAAGATTACCATGAAATTTTGAGGGCTGCAGGATAGATATAGTTTGAGGTGCGGAAGTATTAATATC
CCCACCCCCTATTCAGAGCA

Downstream 100 bases:

>100_bases
GAAAAGGGACGGGTTCTCCAGAACAGGAAAATTCGAGGTTGAAGGTAGAACAGTTCAAACGCCAGCCATACTTGAGGTGG
GAGAGATTCCTGAGTGGGAT

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MQRFEILDKDAMGRICRIETPHGRIETPTILPVINPNIPFIRAEEMKKFGAQAVITNSYIIYRSMREEALEKGVHGILET
DMPVMTDSGSYQLMVYGDVEIKNAEIVEFQRHIGSDIIVPLDIPTPPDADYATAESDLRITLEREREAKELLKGAENLLA
VPVQGSTHPDLRRFAAGEARKIGGDIYPIGAVVPLMDAYRFRDLARVILEVRSALPVEPIHLFGCGHPMLFAMAVALGCD
LFDSAAYALYAKDDRYLTVYGTKKLSELNYFPCKCPVCSNHDPEELRRMEKNERERLIAEHNLYVSFQEIETIKQAIKEN
SLFELVEKRVRAHPNMLAGWRQVKHYWELLEKADPKMKRKFLYTGIDSLYRPAVRRHVKAIKNVELPEEVLVSTDFGIYA
NIYLRPVFGPVPAEMLETYPAGHAEIPEEDVVEEEALKAASEALMELMNSHPEKRFKVYVSKVWMKHLQNLPPNGELNVL
S

Sequences:

>Translated_481_residues
MQRFEILDKDAMGRICRIETPHGRIETPTILPVINPNIPFIRAEEMKKFGAQAVITNSYIIYRSMREEALEKGVHGILET
DMPVMTDSGSYQLMVYGDVEIKNAEIVEFQRHIGSDIIVPLDIPTPPDADYATAESDLRITLEREREAKELLKGAENLLA
VPVQGSTHPDLRRFAAGEARKIGGDIYPIGAVVPLMDAYRFRDLARVILEVRSALPVEPIHLFGCGHPMLFAMAVALGCD
LFDSAAYALYAKDDRYLTVYGTKKLSELNYFPCKCPVCSNHDPEELRRMEKNERERLIAEHNLYVSFQEIETIKQAIKEN
SLFELVEKRVRAHPNMLAGWRQVKHYWELLEKADPKMKRKFLYTGIDSLYRPAVRRHVKAIKNVELPEEVLVSTDFGIYA
NIYLRPVFGPVPAEMLETYPAGHAEIPEEDVVEEEALKAASEALMELMNSHPEKRFKVYVSKVWMKHLQNLPPNGELNVL
S
>Mature_481_residues
MQRFEILDKDAMGRICRIETPHGRIETPTILPVINPNIPFIRAEEMKKFGAQAVITNSYIIYRSMREEALEKGVHGILET
DMPVMTDSGSYQLMVYGDVEIKNAEIVEFQRHIGSDIIVPLDIPTPPDADYATAESDLRITLEREREAKELLKGAENLLA
VPVQGSTHPDLRRFAAGEARKIGGDIYPIGAVVPLMDAYRFRDLARVILEVRSALPVEPIHLFGCGHPMLFAMAVALGCD
LFDSAAYALYAKDDRYLTVYGTKKLSELNYFPCKCPVCSNHDPEELRRMEKNERERLIAEHNLYVSFQEIETIKQAIKEN
SLFELVEKRVRAHPNMLAGWRQVKHYWELLEKADPKMKRKFLYTGIDSLYRPAVRRHVKAIKNVELPEEVLVSTDFGIYA
NIYLRPVFGPVPAEMLETYPAGHAEIPEEDVVEEEALKAASEALMELMNSHPEKRFKVYVSKVWMKHLQNLPPNGELNVL
S

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeosine tRNA-ribosyltransferase family

Homologues:

Organism=Escherichia coli, GI1786607, Length=360, Percent_Identity=25.8333333333333, Blast_Score=109, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17544680, Length=365, Percent_Identity=21.3698630136986, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATGT_ARCFU (O29667)

Other databases:

- EMBL:   AE000782
- PIR:   D69323
- RefSeq:   NP_069422.1
- ProteinModelPortal:   O29667
- SMR:   O29667
- GeneID:   1483805
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0588
- NMPDR:   fig|224325.1.peg.581
- TIGR:   AF_0588
- HOGENOM:   HBG512800
- OMA:   IREGRLW
- ProtClustDB:   PRK13533
- BioCyc:   AFUL224325:AF_0588-MONOMER
- HAMAP:   MF_01634
- InterPro:   IPR004804
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00432
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 54820; Mature: 54820

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50890 PUA

Important sites: ACT_SITE 87-87

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRFEILDKDAMGRICRIETPHGRIETPTILPVINPNIPFIRAEEMKKFGAQAVITNSYI
CCCCHHCCHHHCCCEEEEECCCCCCCCCEEEEEECCCCCEEEHHHHHHCCCCEEEECCCH
IYRSMREEALEKGVHGILETDMPVMTDSGSYQLMVYGDVEIKNAEIVEFQRHIGSDIIVP
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECEEECCCHHHHHHHHCCCCEEEE
LDIPTPPDADYATAESDLRITLEREREAKELLKGAENLLAVPVQGSTHPDLRRFAAGEAR
ECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCHH
KIGGDIYPIGAVVPLMDAYRFRDLARVILEVRSALPVEPIHLFGCGHPMLFAMAVALGCD
HCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCH
LFDSAAYALYAKDDRYLTVYGTKKLSELNYFPCKCPVCSNHDPEELRRMEKNERERLIAE
HHCCCEEEEEECCCCEEEEEECCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HNLYVSFQEIETIKQAIKENSLFELVEKRVRAHPNMLAGWRQVKHYWELLEKADPKMKRK
CCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
FLYTGIDSLYRPAVRRHVKAIKNVELPEEVLVSTDFGIYANIYLRPVFGPVPAEMLETYP
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCEEEEEEEEECCCCCCHHHHHHCC
AGHAEIPEEDVVEEEALKAASEALMELMNSHPEKRFKVYVSKVWMKHLQNLPPNGELNVL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEC
S
C
>Mature Secondary Structure
MQRFEILDKDAMGRICRIETPHGRIETPTILPVINPNIPFIRAEEMKKFGAQAVITNSYI
CCCCHHCCHHHCCCEEEEECCCCCCCCCEEEEEECCCCCEEEHHHHHHCCCCEEEECCCH
IYRSMREEALEKGVHGILETDMPVMTDSGSYQLMVYGDVEIKNAEIVEFQRHIGSDIIVP
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECEEECCCHHHHHHHHCCCCEEEE
LDIPTPPDADYATAESDLRITLEREREAKELLKGAENLLAVPVQGSTHPDLRRFAAGEAR
ECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCHH
KIGGDIYPIGAVVPLMDAYRFRDLARVILEVRSALPVEPIHLFGCGHPMLFAMAVALGCD
HCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCH
LFDSAAYALYAKDDRYLTVYGTKKLSELNYFPCKCPVCSNHDPEELRRMEKNERERLIAE
HHCCCEEEEEECCCCEEEEEECCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HNLYVSFQEIETIKQAIKENSLFELVEKRVRAHPNMLAGWRQVKHYWELLEKADPKMKRK
CCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
FLYTGIDSLYRPAVRRHVKAIKNVELPEEVLVSTDFGIYANIYLRPVFGPVPAEMLETYP
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCEEEEEEEEECCCCCCHHHHHHCC
AGHAEIPEEDVVEEEALKAASEALMELMNSHPEKRFKVYVSKVWMKHLQNLPPNGELNVL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.000014 {7-(aminomethyl)-7-deazaguanine}} 0.00006 {guanine}} 0.000053 {guanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475