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The map label for this gene is trzA-1

Identifier: 11498160

GI number: 11498160

Start: 501786

End: 503054

Strand: Reverse

Name: trzA-1

Synonym: AF0550

Alternate gene names: 11498160

Gene position: 503054-501786 (Counterclockwise)

Preceding gene: 11498170

Following gene: 11498155

Centisome position: 23.09

GC content: 50.43

Gene sequence:

>1269_bases
ATGATTCTCATCAAGAATGCCAAGATTTTGACTCCCAAGGGAATTGTGGAGGGAAATCTGAAGGTTGAAGGGAAAAAGAT
TTCCGAAATAGGCGGTAAGGCTGTGAAGAGCGATGTCGTCATTGACGGCAGCAGGAAAGCTGTTATTCCCGGCTTCTTCA
ACACCCACACCCACGCTGCAATGACTCTCTTCAGGAGCTACGCTGACGACATGCAGCTTCACGAGTGGCTGGAGAAGAAA
ATATGGCCTTTGGAGGCAAAACTGGATGATAAAGCAGTTTACTGGGGAACGAAGCTTGCATGCGTTGAGATGCTGAAGAG
CGGGACGGTGTTTTTCAACGACATGTACTTTTTCCCCGAAGCTATTGCAAGGGCGGCTGAGGAGTGCGGAATAAGGGCAT
GCGTTTCTGCAGCTTTCTTCGACTTCTTCAATCCCGATTTGCTGGAGTTGAACCTGAAGAATGCGGTGAAAAGCCTGAGG
GAGATTGAGAAGTACGATGTGCTTAGAGCGATAGGCCCTCATGCTGTCTACACTGTGAGCCTTGACGGGTTGAGGAGGGC
TGCTGAGATAGCGGAGGAGATGGACATCTTCATGCACTTCCATCTGGCAGAGACGGAAAAGGAGGTTTTAGACTTTAAAA
AGCAGCACGGGAAGCTGATCGTTCAGGCTCTGGATGAAATTGGATTCTTGAGCAAGAGGCTTATCGCTGCCCACAGCGTC
TGGCTGGAGGATGCCGAAATTGAAATTCTGGCGAAGAAGGGGGTGAGCGTTGCCCACTGCCCTGCGAGCAACATGAAGCT
TTGCGTCGGTAAAGCGATAAGGTATGAGGCAATGAAGAGGGCTGGAGTTAACTTCACCCTTGCCACAGATGGTGCGGCAA
GCAACAACAACCTCGACATGCTGGAGGAGATGAAGTTCGCCGCTTTGCTGCAGAAATTCCACCACAGCAACCCAACTCTG
CTAAAAGCGGAGGAGGTTTTCGAGGCTGCAACGCTTAACGGGGCAAAGGCTTTTGGCATAAAGTCTGGAGTAATAAAGGA
GGGCTACGAGGCCGATATTGTTCTGGTGGACTTAGCAAAGCCCTACATGCAGCCAGAGCACAGCCTCATCGCCAATCTTG
TATATGCTGCCTCCTCAGGCTGCGTTGACACTGTAATAGTGAAAGGAGAGGTGGTGGTTGAGGGCGGGGTGTTCAGGAGC
GAGGAGGAGGAGCTCAAAATAATAGAAAAGGCCAATGAAGTTGCAAAAAGGCTCACAGGTTCAGCATAA

Upstream 100 bases:

>100_bases
TAAGATGCTATGAGTATTAATAATTTTTGGTCAGTGTTAAATTCATGGCAGTAATAAAGAGGACAAAAGAATTTTATCCC
AGCAGTCAGGATAATGGCCA

Downstream 100 bases:

>100_bases
TCGTCCAGCTTATGTACCAGCTTGAGAAGGTCAGGAAAGCAATCCCAGCCCAGTCCTTTCCCTCAAGCTCAATCCCCATT
TTTGGCAGGATGAACTTCTG

Product: N-ethylammeline chlorohydrolase (trzA-1)

Products: NA

Alternate protein names: MTA/SAH deaminase 1

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MILIKNAKILTPKGIVEGNLKVEGKKISEIGGKAVKSDVVIDGSRKAVIPGFFNTHTHAAMTLFRSYADDMQLHEWLEKK
IWPLEAKLDDKAVYWGTKLACVEMLKSGTVFFNDMYFFPEAIARAAEECGIRACVSAAFFDFFNPDLLELNLKNAVKSLR
EIEKYDVLRAIGPHAVYTVSLDGLRRAAEIAEEMDIFMHFHLAETEKEVLDFKKQHGKLIVQALDEIGFLSKRLIAAHSV
WLEDAEIEILAKKGVSVAHCPASNMKLCVGKAIRYEAMKRAGVNFTLATDGAASNNNLDMLEEMKFAALLQKFHHSNPTL
LKAEEVFEAATLNGAKAFGIKSGVIKEGYEADIVLVDLAKPYMQPEHSLIANLVYAASSGCVDTVIVKGEVVVEGGVFRS
EEEELKIIEKANEVAKRLTGSA

Sequences:

>Translated_422_residues
MILIKNAKILTPKGIVEGNLKVEGKKISEIGGKAVKSDVVIDGSRKAVIPGFFNTHTHAAMTLFRSYADDMQLHEWLEKK
IWPLEAKLDDKAVYWGTKLACVEMLKSGTVFFNDMYFFPEAIARAAEECGIRACVSAAFFDFFNPDLLELNLKNAVKSLR
EIEKYDVLRAIGPHAVYTVSLDGLRRAAEIAEEMDIFMHFHLAETEKEVLDFKKQHGKLIVQALDEIGFLSKRLIAAHSV
WLEDAEIEILAKKGVSVAHCPASNMKLCVGKAIRYEAMKRAGVNFTLATDGAASNNNLDMLEEMKFAALLQKFHHSNPTL
LKAEEVFEAATLNGAKAFGIKSGVIKEGYEADIVLVDLAKPYMQPEHSLIANLVYAASSGCVDTVIVKGEVVVEGGVFRS
EEEELKIIEKANEVAKRLTGSA
>Mature_422_residues
MILIKNAKILTPKGIVEGNLKVEGKKISEIGGKAVKSDVVIDGSRKAVIPGFFNTHTHAAMTLFRSYADDMQLHEWLEKK
IWPLEAKLDDKAVYWGTKLACVEMLKSGTVFFNDMYFFPEAIARAAEECGIRACVSAAFFDFFNPDLLELNLKNAVKSLR
EIEKYDVLRAIGPHAVYTVSLDGLRRAAEIAEEMDIFMHFHLAETEKEVLDFKKQHGKLIVQALDEIGFLSKRLIAAHSV
WLEDAEIEILAKKGVSVAHCPASNMKLCVGKAIRYEAMKRAGVNFTLATDGAASNNNLDMLEEMKFAALLQKFHHSNPTL
LKAEEVFEAATLNGAKAFGIKSGVIKEGYEADIVLVDLAKPYMQPEHSLIANLVYAASSGCVDTVIVKGEVVVEGGVFRS
EEEELKIIEKANEVAKRLTGSA

Specific function: Catalyzes the deamination of 5-methylthioadenosine and S-adenosyl-L-homocysteine into 5-methylthioinosine and S-inosyl-L- homocysteine, respectively. Is also able to deaminate adenosine

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MTA/SAH deaminase family

Homologues:

Organism=Homo sapiens, GI4758426, Length=333, Percent_Identity=26.1261261261261, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87082177, Length=430, Percent_Identity=26.2790697674419, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789249, Length=414, Percent_Identity=28.2608695652174, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540282, Length=420, Percent_Identity=24.047619047619, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319963, Length=335, Percent_Identity=22.3880597014925, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24643849, Length=384, Percent_Identity=27.0833333333333, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTAD1_ARCFU (O29701)

Other databases:

- EMBL:   AE000782
- PIR:   F69318
- RefSeq:   NP_069386.1
- ProteinModelPortal:   O29701
- SMR:   O29701
- GeneID:   1483766
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0550
- NMPDR:   fig|224325.1.peg.545
- TIGR:   AF_0550
- HOGENOM:   HBG668170
- OMA:   RWIVPVE
- BioCyc:   AFUL224325:AF_0550-MONOMER
- BRENDA:   3.5.4.28
- HAMAP:   MF_01281
- InterPro:   IPR006680
- InterPro:   IPR011059

Pfam domain/function: PF01979 Amidohydro_1; SSF51338 Metalo_hydrolase

EC number: =3.5.4.28

Molecular weight: Translated: 46596; Mature: 46596

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: BINDING 85-85 BINDING 174-174 BINDING 204-204 BINDING 290-290

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIKNAKILTPKGIVEGNLKVEGKKISEIGGKAVKSDVVIDGSRKAVIPGFFNTHTHAA
CEEEECCEEECCCCEEECCEEECCCHHHHCCCHHHCCCEEEECCCCEECCCCCCCHHHHH
MTLFRSYADDMQLHEWLEKKIWPLEAKLDDKAVYWGTKLACVEMLKSGTVFFNDMYFFPE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHCCCEEEEHHHHHHH
AIARAAEECGIRACVSAAFFDFFNPDLLELNLKNAVKSLREIEKYDVLRAIGPHAVYTVS
HHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
LDGLRRAAEIAEEMDIFMHFHLAETEKEVLDFKKQHGKLIVQALDEIGFLSKRLIAAHSV
HHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
WLEDAEIEILAKKGVSVAHCPASNMKLCVGKAIRYEAMKRAGVNFTLATDGAASNNNLDM
HCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHH
LEEMKFAALLQKFHHSNPTLLKAEEVFEAATLNGAKAFGIKSGVIKEGYEADIVLVDLAK
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCEEEEECCC
PYMQPEHSLIANLVYAASSGCVDTVIVKGEVVVEGGVFRSEEEELKIIEKANEVAKRLTG
CCCCCHHHHHHHHHHHCCCCCEEEEEEECEEEEECCCCCCCHHHHHHHHHHHHHHHHHCC
SA
CC
>Mature Secondary Structure
MILIKNAKILTPKGIVEGNLKVEGKKISEIGGKAVKSDVVIDGSRKAVIPGFFNTHTHAA
CEEEECCEEECCCCEEECCEEECCCHHHHCCCHHHCCCEEEECCCCEECCCCCCCHHHHH
MTLFRSYADDMQLHEWLEKKIWPLEAKLDDKAVYWGTKLACVEMLKSGTVFFNDMYFFPE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHCCCEEEEHHHHHHH
AIARAAEECGIRACVSAAFFDFFNPDLLELNLKNAVKSLREIEKYDVLRAIGPHAVYTVS
HHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
LDGLRRAAEIAEEMDIFMHFHLAETEKEVLDFKKQHGKLIVQALDEIGFLSKRLIAAHSV
HHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
WLEDAEIEILAKKGVSVAHCPASNMKLCVGKAIRYEAMKRAGVNFTLATDGAASNNNLDM
HCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHH
LEEMKFAALLQKFHHSNPTLLKAEEVFEAATLNGAKAFGIKSGVIKEGYEADIVLVDLAK
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCEEEEECCC
PYMQPEHSLIANLVYAASSGCVDTVIVKGEVVVEGGVFRSEEEELKIIEKANEVAKRLTG
CCCCCHHHHHHHHHHHCCCCCEEEEEEECEEEEECCCCCCCHHHHHHHHHHHHHHHHHCC
SA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475