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The map label for this gene is cysG-1

Identifier: 11498034

GI number: 11498034

Start: 380608

End: 381339

Strand: Reverse

Name: cysG-1

Synonym: AF0422

Alternate gene names: 11498034

Gene position: 381339-380608 (Counterclockwise)

Preceding gene: 11498040

Following gene: 11498033

Centisome position: 17.51

GC content: 46.99

Gene sequence:

>732_bases
GTGGGTGGAAAGGTTTACATAGTTGGAGCTGGTCCCGGAAACTTCGAGCTTTTAACTCTGAAAGCATACAGGCTGCTTCA
GGAGGCTGATGTAATTCTGTACGACAGGCTAGTTGGGGAGGAAATTGAGTCCTTCCTTAAAACCCTTGGCAAAGAGGTTG
TCTATGTTGGCAAGGAGAGAGGGGAGAAAGGAGAAAAAAGGCAGAAAGAGATAAACAGGCTGATGAAGAAGTACTTTGAA
GAAGGAAAGAAGATCGTGAGACTGAAAGGAGGAGATCCGGGAATTTTCGGCAGAATGGCTGAGGAGATTGAATTTTTGGT
TGAGAATAGAATCCCCTTTGAGGTTGTTCCGGGCGTTAGCTCCGTAAACGGTGCGCCGGTTTGCTCAGGAATTCCCCTTA
CCGAAGCAGAAAACGCATTCGGCTTTCTTGTTGTGAGCGGAAGTGACTCTGGGAGAATAAGAGAACTGCACGAGAAGGGG
ACGCTCGTGATTCTGATGGGGGGCTGCACGGCCAAGGATGTTGCCAGAAACCTGATTGAATCAGGGGTGGATGCAGATAG
AGACGTGGCGGTAATCGAGAGAGGGAGCATGAAAAACCAGAGGGTTACTATTACAAAGCTTTCTGAGCTTGTTGAGAGAG
ATTTTAATTTTGATAGCCCTGCGCTAATCGTCGTTGGGGATGTTGTAAGGTTCGCAAGAGTTTGCCAAAACCATGAGGAT
AGAAAAATATAA

Upstream 100 bases:

>100_bases
GGCAATATATAAGAATTTCGAAATTTGCGCTTTCTGGTTTAAGCTTACCTAAAAATATCTGCAATCGAGAAGTTTTTTAA
CCTTCCCACAACTCCCCCTT

Downstream 100 bases:

>100_bases
TTGACCTGAGGTTAAGAAAAAACAGATGGTAAAGAGAGCCAAGTTGATTAACGATGTGGTGGAGCTAATCCCGATATTTC
ATCTCCTCTCAACCGAAACT

Product: uroporphyrin-III C-methyltransferase (cysG-1)

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MGGKVYIVGAGPGNFELLTLKAYRLLQEADVILYDRLVGEEIESFLKTLGKEVVYVGKERGEKGEKRQKEINRLMKKYFE
EGKKIVRLKGGDPGIFGRMAEEIEFLVENRIPFEVVPGVSSVNGAPVCSGIPLTEAENAFGFLVVSGSDSGRIRELHEKG
TLVILMGGCTAKDVARNLIESGVDADRDVAVIERGSMKNQRVTITKLSELVERDFNFDSPALIVVGDVVRFARVCQNHED
RKI

Sequences:

>Translated_243_residues
MGGKVYIVGAGPGNFELLTLKAYRLLQEADVILYDRLVGEEIESFLKTLGKEVVYVGKERGEKGEKRQKEINRLMKKYFE
EGKKIVRLKGGDPGIFGRMAEEIEFLVENRIPFEVVPGVSSVNGAPVCSGIPLTEAENAFGFLVVSGSDSGRIRELHEKG
TLVILMGGCTAKDVARNLIESGVDADRDVAVIERGSMKNQRVTITKLSELVERDFNFDSPALIVVGDVVRFARVCQNHED
RKI
>Mature_242_residues
GGKVYIVGAGPGNFELLTLKAYRLLQEADVILYDRLVGEEIESFLKTLGKEVVYVGKERGEKGEKRQKEINRLMKKYFEE
GKKIVRLKGGDPGIFGRMAEEIEFLVENRIPFEVVPGVSSVNGAPVCSGIPLTEAENAFGFLVVSGSDSGRIRELHEKGT
LVILMGGCTAKDVARNLIESGVDADRDVAVIERGSMKNQRVTITKLSELVERDFNFDSPALIVVGDVVRFARVCQNHEDR
KI

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=235, Percent_Identity=41.7021276595745, Blast_Score=162, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322922, Length=235, Percent_Identity=34.8936170212766, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 26861; Mature: 26730

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGKVYIVGAGPGNFELLTLKAYRLLQEADVILYDRLVGEEIESFLKTLGKEVVYVGKER
CCCEEEEEECCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHEEEECHHC
GEKGEKRQKEINRLMKKYFEEGKKIVRLKGGDPGIFGRMAEEIEFLVENRIPFEVVPGVS
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCC
SVNGAPVCSGIPLTEAENAFGFLVVSGSDSGRIRELHEKGTLVILMGGCTAKDVARNLIE
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHH
SGVDADRDVAVIERGSMKNQRVTITKLSELVERDFNFDSPALIVVGDVVRFARVCQNHED
CCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHCCCCC
RKI
CCC
>Mature Secondary Structure 
GGKVYIVGAGPGNFELLTLKAYRLLQEADVILYDRLVGEEIESFLKTLGKEVVYVGKER
CCEEEEEECCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHEEEECHHC
GEKGEKRQKEINRLMKKYFEEGKKIVRLKGGDPGIFGRMAEEIEFLVENRIPFEVVPGVS
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCC
SVNGAPVCSGIPLTEAENAFGFLVVSGSDSGRIRELHEKGTLVILMGGCTAKDVARNLIE
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHH
SGVDADRDVAVIERGSMKNQRVTITKLSELVERDFNFDSPALIVVGDVVRFARVCQNHED
CCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHCCCCC
RKI
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA