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The map label for this gene is gltX

Identifier: 11497876

GI number: 11497876

Start: 235906

End: 237561

Strand: Reverse

Name: gltX

Synonym: AF0260

Alternate gene names: 11497876

Gene position: 237561-235906 (Counterclockwise)

Preceding gene: 11497878

Following gene: 11497874

Centisome position: 10.91

GC content: 47.28

Gene sequence:

>1656_bases
GTGAAAGAAGTCATAATGAAATACGTAGTTCAGAACGCCGCAAAGTACGGAAAAGCCAGTGAAAAGGCTGTAATGGGGAA
GGTGATGGCAGAAAATCCAGAACTCAGAAAAAAAGCTAAAGAGGTCCTTGAACTCGTAAAGGAGTGCATTACCGAGTTCG
AAGCGCTTTCTGAAGAAGTAAGGAAGGAGCTAATCAAAAAATACAGCATGGATAGCGAAGCTAAAAGGGAGTTGGAGACG
AAGAAGCTTCCAGAGCTTGAGGGGGCTGAGAAAGGGAAAGTTGTGATGAGATTCGCTCCTAATCCAAACGGTCCACCTAC
ACTCGGTTCTGCTAGGGGGATAATCGTCAACGGTGAATACGCGAAGATGTACGAAGGGAAGTACATTATCAGGTTTGACG
ATACAGACCCCAGAACCAAGAGACCGATGATTGAGGCCTACGAGTGGTACCTAGAGGACATTGAATGGCTCGGTTACAAG
CCTGACGAGGTTATTTACGCCTCAAGAAGGATTCCCATCTACTACGATTATGCAAGAAAGCTTATAGAAATGGGCAAAGC
CTACACCTGCTTCTGCAGTCAGGAGGAGTTCAAGAAGTTCAGGGACAGTGGTGAGGAGTGCCCACACAGAAACATCAGCG
TCGAGGATACACTTGAAGTCTGGGAGAGAATGCTTGAAGGAGACTATGAGGAAGGAGAAGTCGTTCTTAGAATTAAGACG
GATATGCGCCACAAGGATCCGGCAATAAGGGACTGGGTGGCTTTCAGGATAATAAAGGAATCACATCCTCTCGTTGGGGA
TAAATATGTTGTCTATCCCACACTCGATTTTGAATCTGCTATAGAGGATCACCTTTTAGGCATAACGCACATTATCAGGG
GTAAGGACTTAATTGATTCTGAGAGGAGGCAGAGATACATCTACGAGTATTTTGGCTGGATTTACCCCATTACAAAGCAC
TGGGGCAGGGTTAAAATCTTCGAATTCGGGAAGCTATCGACTTCTTCAATTAAGAAGGATATTGAAAGGGGTAAGTATGA
GGGCTGGGATGACCCAAGGCTGCCAACCTTGAGAGCCTTCAGGAGGAGAGGATTTGAGCCTGAAGCCATAAAGAGCTTCT
TCCTTTCGCTGGGAGTTGGCGAGAACGACGTTTCCGTCAGTCTCAAGAATCTTTACGCTGAGAACAGGAAAATCATCGAT
CGCAAGGCAAACCGTTACTTTTTCATTTGGGGGCCCGTGAAGATTGAAATCGTTAACCTGCCGGAGAAGAAAGAGGTCGA
GCTCCCGCTGAATCCGCACACGGGCGAGAAGAGAAGGCTGAAAGGTGAAAGAACTATATATGTTACAAAAGACGACTTCG
AGAGGTTGAAAGGGCAGGTTGTAAGGCTGAAGGACTTCTGCAACGTTCTGCTTGACGAGAAGGCAGAGTTCATGGGTTTT
GAGCTTGAGGGGGTCAAGAAGGGGAAGAACATAATCCACTGGCTTCCTGAGAGCGAGGCTATCAAGGGCAAGGTTATCGG
CGAAAGGGAAGCGGAAGGGCTTGTGGAGAGAAACGCAGTTAGAGATGTCGGGAAAGTCGTGCAGTTCGAGAGATTTGCTT
TCTGTAAAGTAGAGTCTGCTGACGAGGAACTGGTCGCGGTGTACACGCACCCGTGA

Upstream 100 bases:

>100_bases
ACAGTGCCAAACTGCTCGATGCCACCATAGGCACGAAGTTTTGCTCTCCTGAATTTAAAACTTTCATCACCTTTATATTT
CTCAACTTCCCAGGTGGGCC

Downstream 100 bases:

>100_bases
CCAAAATCCTTAAAAGCCCAATGAGATTAAGGTCGAAAGAGCCACAAGCCCGGGTGGTGTAGTCCGGCCTATCATGCGGG
CCTGTCGTGGAGATGGGGTC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MKEVIMKYVVQNAAKYGKASEKAVMGKVMAENPELRKKAKEVLELVKECITEFEALSEEVRKELIKKYSMDSEAKRELET
KKLPELEGAEKGKVVMRFAPNPNGPPTLGSARGIIVNGEYAKMYEGKYIIRFDDTDPRTKRPMIEAYEWYLEDIEWLGYK
PDEVIYASRRIPIYYDYARKLIEMGKAYTCFCSQEEFKKFRDSGEECPHRNISVEDTLEVWERMLEGDYEEGEVVLRIKT
DMRHKDPAIRDWVAFRIIKESHPLVGDKYVVYPTLDFESAIEDHLLGITHIIRGKDLIDSERRQRYIYEYFGWIYPITKH
WGRVKIFEFGKLSTSSIKKDIERGKYEGWDDPRLPTLRAFRRRGFEPEAIKSFFLSLGVGENDVSVSLKNLYAENRKIID
RKANRYFFIWGPVKIEIVNLPEKKEVELPLNPHTGEKRRLKGERTIYVTKDDFERLKGQVVRLKDFCNVLLDEKAEFMGF
ELEGVKKGKNIIHWLPESEAIKGKVIGEREAEGLVERNAVRDVGKVVQFERFAFCKVESADEELVAVYTHP

Sequences:

>Translated_551_residues
MKEVIMKYVVQNAAKYGKASEKAVMGKVMAENPELRKKAKEVLELVKECITEFEALSEEVRKELIKKYSMDSEAKRELET
KKLPELEGAEKGKVVMRFAPNPNGPPTLGSARGIIVNGEYAKMYEGKYIIRFDDTDPRTKRPMIEAYEWYLEDIEWLGYK
PDEVIYASRRIPIYYDYARKLIEMGKAYTCFCSQEEFKKFRDSGEECPHRNISVEDTLEVWERMLEGDYEEGEVVLRIKT
DMRHKDPAIRDWVAFRIIKESHPLVGDKYVVYPTLDFESAIEDHLLGITHIIRGKDLIDSERRQRYIYEYFGWIYPITKH
WGRVKIFEFGKLSTSSIKKDIERGKYEGWDDPRLPTLRAFRRRGFEPEAIKSFFLSLGVGENDVSVSLKNLYAENRKIID
RKANRYFFIWGPVKIEIVNLPEKKEVELPLNPHTGEKRRLKGERTIYVTKDDFERLKGQVVRLKDFCNVLLDEKAEFMGF
ELEGVKKGKNIIHWLPESEAIKGKVIGEREAEGLVERNAVRDVGKVVQFERFAFCKVESADEELVAVYTHP
>Mature_551_residues
MKEVIMKYVVQNAAKYGKASEKAVMGKVMAENPELRKKAKEVLELVKECITEFEALSEEVRKELIKKYSMDSEAKRELET
KKLPELEGAEKGKVVMRFAPNPNGPPTLGSARGIIVNGEYAKMYEGKYIIRFDDTDPRTKRPMIEAYEWYLEDIEWLGYK
PDEVIYASRRIPIYYDYARKLIEMGKAYTCFCSQEEFKKFRDSGEECPHRNISVEDTLEVWERMLEGDYEEGEVVLRIKT
DMRHKDPAIRDWVAFRIIKESHPLVGDKYVVYPTLDFESAIEDHLLGITHIIRGKDLIDSERRQRYIYEYFGWIYPITKH
WGRVKIFEFGKLSTSSIKKDIERGKYEGWDDPRLPTLRAFRRRGFEPEAIKSFFLSLGVGENDVSVSLKNLYAENRKIID
RKANRYFFIWGPVKIEIVNLPEKKEVELPLNPHTGEKRRLKGERTIYVTKDDFERLKGQVVRLKDFCNVLLDEKAEFMGF
ELEGVKKGKNIIHWLPESEAIKGKVIGEREAEGLVERNAVRDVGKVVQFERFAFCKVESADEELVAVYTHP

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI62241042, Length=363, Percent_Identity=34.9862258953168, Blast_Score=205, Evalue=1e-52,
Organism=Homo sapiens, GI4826960, Length=401, Percent_Identity=31.1720698254364, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI134288884, Length=331, Percent_Identity=27.190332326284, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1786895, Length=451, Percent_Identity=29.0465631929047, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788743, Length=305, Percent_Identity=26.8852459016393, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17511007, Length=391, Percent_Identity=31.4578005115089, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17543332, Length=374, Percent_Identity=31.8181818181818, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71993394, Length=261, Percent_Identity=29.8850574712644, Blast_Score=95, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324742, Length=379, Percent_Identity=34.3007915567282, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6321192, Length=437, Percent_Identity=31.8077803203661, Blast_Score=194, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6324540, Length=335, Percent_Identity=28.955223880597, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17864488, Length=371, Percent_Identity=33.4231805929919, Blast_Score=195, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24649466, Length=396, Percent_Identity=32.5757575757576, Blast_Score=193, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24665321, Length=313, Percent_Identity=28.4345047923323, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_ARCFU (O29979)

Other databases:

- EMBL:   AE000782
- PIR:   D69282
- RefSeq:   NP_069098.1
- ProteinModelPortal:   O29979
- GeneID:   1483474
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0260
- NMPDR:   fig|224325.1.peg.257
- TIGR:   AF_0260
- HOGENOM:   HBG334108
- OMA:   MRFAPNP
- ProtClustDB:   PRK04156
- BioCyc:   AFUL224325:AF_0260-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_A
- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00463

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.17

Molecular weight: Translated: 64403; Mature: 64403

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVIMKYVVQNAAKYGKASEKAVMGKVMAENPELRKKAKEVLELVKECITEFEALSEEV
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKELIKKYSMDSEAKRELETKKLPELEGAEKGKVVMRFAPNPNGPPTLGSARGIIVNGEY
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCC
AKMYEGKYIIRFDDTDPRTKRPMIEAYEWYLEDIEWLGYKPDEVIYASRRIPIYYDYARK
CEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEHHHHHH
LIEMGKAYTCFCSQEEFKKFRDSGEECPHRNISVEDTLEVWERMLEGDYEEGEVVLRIKT
HHHCCCCEEEEECHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEC
DMRHKDPAIRDWVAFRIIKESHPLVGDKYVVYPTLDFESAIEDHLLGITHIIRGKDLIDS
CCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHCH
ERRQRYIYEYFGWIYPITKHWGRVKIFEFGKLSTSSIKKDIERGKYEGWDDPRLPTLRAF
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHH
RRRGFEPEAIKSFFLSLGVGENDVSVSLKNLYAENRKIIDRKANRYFFIWGPVKIEIVNL
HHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEC
PEKKEVELPLNPHTGEKRRLKGERTIYVTKDDFERLKGQVVRLKDFCNVLLDEKAEFMGF
CCCCCEECCCCCCCCCHHHCCCCEEEEEECHHHHHHHHHEEHHHHHHHHHHHCCHHHCCE
ELEGVKKGKNIIHWLPESEAIKGKVIGEREAEGLVERNAVRDVGKVVQFERFAFCKVESA
EECCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
DEELVAVYTHP
CCCEEEEEECC
>Mature Secondary Structure
MKEVIMKYVVQNAAKYGKASEKAVMGKVMAENPELRKKAKEVLELVKECITEFEALSEEV
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKELIKKYSMDSEAKRELETKKLPELEGAEKGKVVMRFAPNPNGPPTLGSARGIIVNGEY
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCC
AKMYEGKYIIRFDDTDPRTKRPMIEAYEWYLEDIEWLGYKPDEVIYASRRIPIYYDYARK
CEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEHHHHHH
LIEMGKAYTCFCSQEEFKKFRDSGEECPHRNISVEDTLEVWERMLEGDYEEGEVVLRIKT
HHHCCCCEEEEECHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEC
DMRHKDPAIRDWVAFRIIKESHPLVGDKYVVYPTLDFESAIEDHLLGITHIIRGKDLIDS
CCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHCH
ERRQRYIYEYFGWIYPITKHWGRVKIFEFGKLSTSSIKKDIERGKYEGWDDPRLPTLRAF
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHH
RRRGFEPEAIKSFFLSLGVGENDVSVSLKNLYAENRKIIDRKANRYFFIWGPVKIEIVNL
HHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEC
PEKKEVELPLNPHTGEKRRLKGERTIYVTKDDFERLKGQVVRLKDFCNVLLDEKAEFMGF
CCCCCEECCCCCCCCCHHHCCCCEEEEEECHHHHHHHHHEEHHHHHHHHHHHCCHHHCCE
ELEGVKKGKNIIHWLPESEAIKGKVIGEREAEGLVERNAVRDVGKVVQFERFAFCKVESA
EECCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
DEELVAVYTHP
CCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475