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The map label for this gene is pheA

Identifier: 11497843

GI number: 11497843

Start: 203791

End: 205653

Strand: Reverse

Name: pheA

Synonym: AF0227

Alternate gene names: 11497843

Gene position: 205653-203791 (Counterclockwise)

Preceding gene: 11497844

Following gene: 11497838

Centisome position: 9.44

GC content: 50.35

Gene sequence:

>1863_bases
GTGATTTCTGTGAAGATTCTTATCTACGGCGTTGGAAACATGGGAAAGCTTTTCAGAGACATCTTCTACGGCAAGGGATA
CTACGTCAGGGGATACGACATAGACCAGATGAAGAGAGACACAAACAGCATTTCAGGTTTTGACGTCATATTCGTCTGCA
CTCCCATGTATGCTCTTGAAGAGGCCTTAGAGCACATAAAGAGGGAGGCGAAAAAGGAGGCCTTGCTTGTGGACGTTTCG
TCTGTCAAGAAGGTTTCCGTTCCGCTGTTTGAGGAATCTGGATTCGACTTCCTGAGCATCCATCCGATGCTTGGCGGGGA
CAGCGAGATTTCCCTCTCAAACGTAATAGTCGTTAGGGAGTCGGGAAGGGAGGAGGAGAAGGTAATCCTCGAGGAGCTCA
GGAAATGCGGAGCCGTGCTCAGCAGGCTCGACGTTGAGGAGCACGACAGGAAGATGGCCGAGATTCAGGGGATAGCTCAC
TTCGCTCTCGTTTCAATGGCCGACTTTCTCAGGTACGGCAAGGAAGAGCTCAAATACGCATCACCGATTTTCACCGTCCT
CTACAAGCTCGCAAGCAGAATAATAAACCAGAACTGGGAGATGTATTTCCAGATTCAGAAGAACGCCGAGGATGTCAGGG
AGGAGTATCTCAGAAGGGCTATGGAGTTGCACGAAAAAATGAAGGACAGAGAGAGCTTCAGAGAAATCTTCGAGAGTTTG
AGAAAAATCTACACCGACTACGAGTCGAGCACGATTATCCTCGAATCCTACAAAGCCACCAAAAAGGCTGAAAGCATAGA
GGAGCTTCGGGGGCTGATAAAGTCCATAGACTCGCTGATTCTGAGGCTCATAGAGAGGAGAATCGACGCGGCAAGGCAGA
TTGCGAGGATAAAGATGGAGCGGGGGGAGCCGATTGAGCTGAAGGATGTGGAGGAGGAGAAGCTCTGGGAGGTTATGTCG
AAAACTACACTGAATCCGGTTAAGCTGAAGGAGATTTTTGAGGGAATAATGAGCCTCGCAAAGGAGGAGGAGTACAAGGT
AGCGGGAGTGAAGTATACAATCGCCGTTCTCGGTCCTCAGGGAAGTTTCAGCGAGGAGATGGCTTTGAAGCTCGTTGGCT
CACGCGTCCCGCTTCGCTACTGCTCCACCACAGATGAAATAATCAAGCTCGTTGAGAGCGGTGAGGTGGACTACGGCCTT
GTTCCGATAGAGAACTCCGTCAACGGAACGGTTTTGCCGGTCATCGATGCTCTGCTGAACCACGATGTTGAGGTCTTCGG
AGAGGCTAAGCTTGAGGTCAACCACTGCCTCGTTGCAAAGAGAAAAATAGAGCTGAAGGAGATAAAAACAATTTACTCCC
ATCCCCAGGCTGTCGCTCAGTGCATGGGCTTCATAAACAACTACCTGCCGTCGGTTGCGATAAGATACACGACATCAACC
AGCGATGCAGCGAGGATGCTCGATGACTATTCGGCAGCGATAATGTCTGAGAACGCCGCAAGGTTTTACAGGCTGCACGT
GCTCAGAAAGGGAATACAGGATTTGAAAGGCAGGAACATAACGAGGTTCTACCTCATAAGAAGAAGGTCGGGAAGGAGTG
AGGGGAAGATAACCTCCCTCTTCTTCGGAGTTGAAGATAAGCCGGGAGCACTGAAGGATGTGCTGGAGGTCTTCCACAAA
AAGGGGTTTAACCTCAGAAAGCTCGAATCGAGGCCTGCCGGAACGGGTCTGGGGGACTACGTCTTCTTCGTTGAGGTAGA
GGCGCCTCTGAGGGAAGAGGACCTGTTGGACTTGAAACAGGTCACGACCTTCTACAAAGTGGTTGGAGTTTTTGATGAGG
TCAAGAGGATGAGCACCCTCTAA

Upstream 100 bases:

>100_bases
CTTACTTAGGCTCTCCATTCATTTACTGCTATGTTGGAAGCCCAAAGGCGCCTGGGCAGATAAGCCTGGATGATGCGAGG
GAAATAATCAGCAGGCTGGG

Downstream 100 bases:

>100_bases
TCACCATTTTTCGAAGTGCGTGAGCATGCTCACCGGATACCTGTTTCTTGGAGACGGATTCTCCGCTGGGTGGCCAATTG
GAATTATCGCCATAGGTCTC

Product: chorismate mutase/prephenate dehydratase (pheA)

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MISVKILIYGVGNMGKLFRDIFYGKGYYVRGYDIDQMKRDTNSISGFDVIFVCTPMYALEEALEHIKREAKKEALLVDVS
SVKKVSVPLFEESGFDFLSIHPMLGGDSEISLSNVIVVRESGREEEKVILEELRKCGAVLSRLDVEEHDRKMAEIQGIAH
FALVSMADFLRYGKEELKYASPIFTVLYKLASRIINQNWEMYFQIQKNAEDVREEYLRRAMELHEKMKDRESFREIFESL
RKIYTDYESSTIILESYKATKKAESIEELRGLIKSIDSLILRLIERRIDAARQIARIKMERGEPIELKDVEEEKLWEVMS
KTTLNPVKLKEIFEGIMSLAKEEEYKVAGVKYTIAVLGPQGSFSEEMALKLVGSRVPLRYCSTTDEIIKLVESGEVDYGL
VPIENSVNGTVLPVIDALLNHDVEVFGEAKLEVNHCLVAKRKIELKEIKTIYSHPQAVAQCMGFINNYLPSVAIRYTTST
SDAARMLDDYSAAIMSENAARFYRLHVLRKGIQDLKGRNITRFYLIRRRSGRSEGKITSLFFGVEDKPGALKDVLEVFHK
KGFNLRKLESRPAGTGLGDYVFFVEVEAPLREEDLLDLKQVTTFYKVVGVFDEVKRMSTL

Sequences:

>Translated_620_residues
MISVKILIYGVGNMGKLFRDIFYGKGYYVRGYDIDQMKRDTNSISGFDVIFVCTPMYALEEALEHIKREAKKEALLVDVS
SVKKVSVPLFEESGFDFLSIHPMLGGDSEISLSNVIVVRESGREEEKVILEELRKCGAVLSRLDVEEHDRKMAEIQGIAH
FALVSMADFLRYGKEELKYASPIFTVLYKLASRIINQNWEMYFQIQKNAEDVREEYLRRAMELHEKMKDRESFREIFESL
RKIYTDYESSTIILESYKATKKAESIEELRGLIKSIDSLILRLIERRIDAARQIARIKMERGEPIELKDVEEEKLWEVMS
KTTLNPVKLKEIFEGIMSLAKEEEYKVAGVKYTIAVLGPQGSFSEEMALKLVGSRVPLRYCSTTDEIIKLVESGEVDYGL
VPIENSVNGTVLPVIDALLNHDVEVFGEAKLEVNHCLVAKRKIELKEIKTIYSHPQAVAQCMGFINNYLPSVAIRYTTST
SDAARMLDDYSAAIMSENAARFYRLHVLRKGIQDLKGRNITRFYLIRRRSGRSEGKITSLFFGVEDKPGALKDVLEVFHK
KGFNLRKLESRPAGTGLGDYVFFVEVEAPLREEDLLDLKQVTTFYKVVGVFDEVKRMSTL
>Mature_620_residues
MISVKILIYGVGNMGKLFRDIFYGKGYYVRGYDIDQMKRDTNSISGFDVIFVCTPMYALEEALEHIKREAKKEALLVDVS
SVKKVSVPLFEESGFDFLSIHPMLGGDSEISLSNVIVVRESGREEEKVILEELRKCGAVLSRLDVEEHDRKMAEIQGIAH
FALVSMADFLRYGKEELKYASPIFTVLYKLASRIINQNWEMYFQIQKNAEDVREEYLRRAMELHEKMKDRESFREIFESL
RKIYTDYESSTIILESYKATKKAESIEELRGLIKSIDSLILRLIERRIDAARQIARIKMERGEPIELKDVEEEKLWEVMS
KTTLNPVKLKEIFEGIMSLAKEEEYKVAGVKYTIAVLGPQGSFSEEMALKLVGSRVPLRYCSTTDEIIKLVESGEVDYGL
VPIENSVNGTVLPVIDALLNHDVEVFGEAKLEVNHCLVAKRKIELKEIKTIYSHPQAVAQCMGFINNYLPSVAIRYTTST
SDAARMLDDYSAAIMSENAARFYRLHVLRKGIQDLKGRNITRFYLIRRRSGRSEGKITSLFFGVEDKPGALKDVLEVFHK
KGFNLRKLESRPAGTGLGDYVFFVEVEAPLREEDLLDLKQVTTFYKVVGVFDEVKRMSTL

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=369, Percent_Identity=29.2682926829268, Blast_Score=149, Evalue=8e-37,
Organism=Escherichia coli, GI1788952, Length=258, Percent_Identity=29.4573643410853, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324013, Length=296, Percent_Identity=27.3648648648649, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010957
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01842 ACT; PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 70948; Mature: 70948

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVKILIYGVGNMGKLFRDIFYGKGYYVRGYDIDQMKRDTNSISGFDVIFVCTPMYALE
CEEEEEEEEECCCHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCCCEEEEECCCHHHHH
EALEHIKREAKKEALLVDVSSVKKVSVPLFEESGFDFLSIHPMLGGDSEISLSNVIVVRE
HHHHHHHHHHHHHEEEEEHHHHHHEECCEECCCCCCEEEECCCCCCCCCEEECEEEEEEC
SGREEEKVILEELRKCGAVLSRLDVEEHDRKMAEIQGIAHFALVSMADFLRYGKEELKYA
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
SPIFTVLYKLASRIINQNWEMYFQIQKNAEDVREEYLRRAMELHEKMKDRESFREIFESL
HHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKIYTDYESSTIILESYKATKKAESIEELRGLIKSIDSLILRLIERRIDAARQIARIKME
HHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGEPIELKDVEEEKLWEVMSKTTLNPVKLKEIFEGIMSLAKEEEYKVAGVKYTIAVLGPQ
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEECEEEEEEEECCC
GSFSEEMALKLVGSRVPLRYCSTTDEIIKLVESGEVDYGLVPIENSVNGTVLPVIDALLN
CCCHHHHHHHHHCCCCCCHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHC
HDVEVFGEAKLEVNHCLVAKRKIELKEIKTIYSHPQAVAQCMGFINNYLPSVAIRYTTST
CCHHEECCCCEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEEECCC
SDAARMLDDYSAAIMSENAARFYRLHVLRKGIQDLKGRNITRFYLIRRRSGRSEGKITSL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEE
FFGVEDKPGALKDVLEVFHKKGFNLRKLESRPAGTGLGDYVFFVEVEAPLREEDLLDLKQ
EECCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHH
VTTFYKVVGVFDEVKRMSTL
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISVKILIYGVGNMGKLFRDIFYGKGYYVRGYDIDQMKRDTNSISGFDVIFVCTPMYALE
CEEEEEEEEECCCHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCCCEEEEECCCHHHHH
EALEHIKREAKKEALLVDVSSVKKVSVPLFEESGFDFLSIHPMLGGDSEISLSNVIVVRE
HHHHHHHHHHHHHEEEEEHHHHHHEECCEECCCCCCEEEECCCCCCCCCEEECEEEEEEC
SGREEEKVILEELRKCGAVLSRLDVEEHDRKMAEIQGIAHFALVSMADFLRYGKEELKYA
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
SPIFTVLYKLASRIINQNWEMYFQIQKNAEDVREEYLRRAMELHEKMKDRESFREIFESL
HHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKIYTDYESSTIILESYKATKKAESIEELRGLIKSIDSLILRLIERRIDAARQIARIKME
HHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGEPIELKDVEEEKLWEVMSKTTLNPVKLKEIFEGIMSLAKEEEYKVAGVKYTIAVLGPQ
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEECEEEEEEEECCC
GSFSEEMALKLVGSRVPLRYCSTTDEIIKLVESGEVDYGLVPIENSVNGTVLPVIDALLN
CCCHHHHHHHHHCCCCCCHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHC
HDVEVFGEAKLEVNHCLVAKRKIELKEIKTIYSHPQAVAQCMGFINNYLPSVAIRYTTST
CCHHEECCCCEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEEECCC
SDAARMLDDYSAAIMSENAARFYRLHVLRKGIQDLKGRNITRFYLIRRRSGRSEGKITSL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEE
FFGVEDKPGALKDVLEVFHKKGFNLRKLESRPAGTGLGDYVFFVEVEAPLREEDLLDLKQ
EECCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHH
VTTFYKVVGVFDEVKRMSTL
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]