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Identifier: 11497798

GI number: 11497798

Start: 160990

End: 162162

Strand: Reverse

Name: Not Available

Synonym: AF0181

Alternate gene names: NA

Gene position: 162162-160990 (Counterclockwise)

Preceding gene: 11497799

Following gene: 11497797

Centisome position: 7.44

GC content: 51.15

Gene sequence:

>1173_bases
ATGTTAAGTAAGCCTATGGAACTGCTGAGACCATCAAAGGGAATGATTAACGTTCATCCCATTCAAAGAGGAGGAATTCT
GACTGAAGAGGCGAGGAAGGTGCTTTTAGAGTGGGGGGATGGCTACAGCATGTGTGATATCTGCCTTGAGGGCAGAGTTG
ACCTGCTGGACAAACCGCCAGTCAAGAGGTTCAAGGAAGAGGTTGCGGAGTTTCTCGGTATGGATGAAGTGAGGTTCACC
GCTGGGGCGAGGCATGCGAAGTTCGTGGCGATGAGCGGGTTTAAGGGGGCTTTGGTGGTGGACTCCCTCGCCCACTACAC
TACCTACATTGCAGCAGAACTCAATGGCCTGAGAGTTTACGAGGTTCCCAACACGGGCTATCCTGAGTTCAGGATAGAGG
CTGAGAGTTATACGGATGTTTTCGAGAAGGTTAAGGAGGAGACCGGAGATTATCCCGCCGTTGCACTGCTAACCCACGCC
GACTACAAATACGGGAATCTGGCAGATGCTAAGAAGGTTGCGGAAATATGCAGGGATTACGGGATTCCGCTTATTTTGAA
CACAGCCTACACCTCAGGGATTATGGAGGTGAGTGGAAGGGAAACGGGATGCGACTTTATTGTTGCAAGCGGGCACAAAA
GCTGGGCCGCAACAGCACCCATCGGAATTCTCGCAACAACTTTTGAGTTCGCTGAAAGGGTTTTCAGGGTTTCTGAAGTG
AGGGGAAACTGGAGCGGCAGGGCTTTCACCAAGAAGGAGGTTGCCCTTTTCGGCTGCTCTCCAGTTTACGGCCTTCCCCT
TGTAACCCTTATGGCCTCCTTTCCAGTAGTAAAGGAGAGGGTGAAGAGATGGAAGGAGGAGGTTGAGAAGGCAAGGTGGT
TTGTTGAGGAGATGGAAAAGATTGAGGGTGTTATGCTTCTGGGGGAGAGGCCGAAGAACCACACGCTCATGAACTTCGAA
ACTCCGTCCTTTAACTTAATAGCTAAAAAGCACAGGCGGAAGGGATACTTCCTCTATCACGAGCTTAAGGAGAGGGGAAT
TTTTGGTGTGCAGCCGGGAATGACGAGGAACGTTAAGCTCAACGTTTACGGCCTGAGCTGGGAGGAGGTTGAGAGGGTTG
CTGAGGCCTTCAAGGAGATTGCCGAGAAATATGGCCTGGAGGTGGAAGATTGA

Upstream 100 bases:

>100_bases
CCGAAATTGATTTCGAATTTTCCCCCTCAGGAAACTTTTACTTGGACTGTCTGAACATCGGAAGGAAACTGCTTCGGGAT
AAAACTTAAAAAACTTAACA

Downstream 100 bases:

>100_bases
GCTTCAGGAGGTATTTTCAGCTCAGATACAGCCCTGTTGGAGTGAAGCTGCTTGAAACCGAGGATGGAGAAGTCATGGAC
AGGTTCTGCTCCGCAGTGAG

Product: Sep-tRNA:Cys-tRNA synthetase

Products: NA

Alternate protein names: Sep-tRNA:Cys-tRNA synthase 2; SepCysS 2

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLSKPMELLRPSKGMINVHPIQRGGILTEEARKVLLEWGDGYSMCDICLEGRVDLLDKPPVKRFKEEVAEFLGMDEVRFT
AGARHAKFVAMSGFKGALVVDSLAHYTTYIAAELNGLRVYEVPNTGYPEFRIEAESYTDVFEKVKEETGDYPAVALLTHA
DYKYGNLADAKKVAEICRDYGIPLILNTAYTSGIMEVSGRETGCDFIVASGHKSWAATAPIGILATTFEFAERVFRVSEV
RGNWSGRAFTKKEVALFGCSPVYGLPLVTLMASFPVVKERVKRWKEEVEKARWFVEEMEKIEGVMLLGERPKNHTLMNFE
TPSFNLIAKKHRRKGYFLYHELKERGIFGVQPGMTRNVKLNVYGLSWEEVERVAEAFKEIAEKYGLEVED

Sequences:

>Translated_390_residues
MLSKPMELLRPSKGMINVHPIQRGGILTEEARKVLLEWGDGYSMCDICLEGRVDLLDKPPVKRFKEEVAEFLGMDEVRFT
AGARHAKFVAMSGFKGALVVDSLAHYTTYIAAELNGLRVYEVPNTGYPEFRIEAESYTDVFEKVKEETGDYPAVALLTHA
DYKYGNLADAKKVAEICRDYGIPLILNTAYTSGIMEVSGRETGCDFIVASGHKSWAATAPIGILATTFEFAERVFRVSEV
RGNWSGRAFTKKEVALFGCSPVYGLPLVTLMASFPVVKERVKRWKEEVEKARWFVEEMEKIEGVMLLGERPKNHTLMNFE
TPSFNLIAKKHRRKGYFLYHELKERGIFGVQPGMTRNVKLNVYGLSWEEVERVAEAFKEIAEKYGLEVED
>Mature_390_residues
MLSKPMELLRPSKGMINVHPIQRGGILTEEARKVLLEWGDGYSMCDICLEGRVDLLDKPPVKRFKEEVAEFLGMDEVRFT
AGARHAKFVAMSGFKGALVVDSLAHYTTYIAAELNGLRVYEVPNTGYPEFRIEAESYTDVFEKVKEETGDYPAVALLTHA
DYKYGNLADAKKVAEICRDYGIPLILNTAYTSGIMEVSGRETGCDFIVASGHKSWAATAPIGILATTFEFAERVFRVSEV
RGNWSGRAFTKKEVALFGCSPVYGLPLVTLMASFPVVKERVKRWKEEVEKARWFVEEMEKIEGVMLLGERPKNHTLMNFE
TPSFNLIAKKHRRKGYFLYHELKERGIFGVQPGMTRNVKLNVYGLSWEEVERVAEAFKEIAEKYGLEVED

Specific function: Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys))

COG id: COG1103

COG function: function code R; Archaea-specific pyridoxal phosphate-dependent enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SepCysS family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPSS2_ARCFU (O30056)

Other databases:

- EMBL:   AE000782
- PIR:   E69272
- RefSeq:   NP_069020.1
- ProteinModelPortal:   O30056
- SMR:   O30056
- GeneID:   1483392
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0181
- NMPDR:   fig|224325.1.peg.179
- TIGR:   AF_0181
- HOGENOM:   HBG539014
- OMA:   AHYTEFL
- ProtClustDB:   PRK09331
- BioCyc:   AFUL224325:AF_0181-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_01675
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR013375
- InterPro:   IPR008829
- Gene3D:   G3DSA:3.40.640.10
- TIGRFAMs:   TIGR02539

Pfam domain/function: PF05889 SLA_LP_auto_ag; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.5.1.73

Molecular weight: Translated: 44127; Mature: 44127

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: BINDING 84-84 BINDING 105-105 BINDING 106-106 BINDING 187-187 BINDING 280-280

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKPMELLRPSKGMINVHPIQRGGILTEEARKVLLEWGDGYSMCDICLEGRVDLLDKPP
CCCCCHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCC
VKRFKEEVAEFLGMDEVRFTAGARHAKFVAMSGFKGALVVDSLAHYTTYIAAELNGLRVY
HHHHHHHHHHHHCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEECCCEEEE
EVPNTGYPEFRIEAESYTDVFEKVKEETGDYPAVALLTHADYKYGNLADAKKVAEICRDY
ECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHC
GIPLILNTAYTSGIMEVSGRETGCDFIVASGHKSWAATAPIGILATTFEFAERVFRVSEV
CCCEEEECHHHCCEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RGNWSGRAFTKKEVALFGCSPVYGLPLVTLMASFPVVKERVKRWKEEVEKARWFVEEMEK
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
IEGVMLLGERPKNHTLMNFETPSFNLIAKKHRRKGYFLYHELKERGIFGVQPGMTRNVKL
HCCEEEECCCCCCCEEEEECCCCCHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCCCEEEE
NVYGLSWEEVERVAEAFKEIAEKYGLEVED
EEEECCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLSKPMELLRPSKGMINVHPIQRGGILTEEARKVLLEWGDGYSMCDICLEGRVDLLDKPP
CCCCCHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCC
VKRFKEEVAEFLGMDEVRFTAGARHAKFVAMSGFKGALVVDSLAHYTTYIAAELNGLRVY
HHHHHHHHHHHHCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEECCCEEEE
EVPNTGYPEFRIEAESYTDVFEKVKEETGDYPAVALLTHADYKYGNLADAKKVAEICRDY
ECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHC
GIPLILNTAYTSGIMEVSGRETGCDFIVASGHKSWAATAPIGILATTFEFAERVFRVSEV
CCCEEEECHHHCCEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RGNWSGRAFTKKEVALFGCSPVYGLPLVTLMASFPVVKERVKRWKEEVEKARWFVEEMEK
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
IEGVMLLGERPKNHTLMNFETPSFNLIAKKHRRKGYFLYHELKERGIFGVQPGMTRNVKL
HCCEEEECCCCCCCEEEEECCCCCHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCCCEEEE
NVYGLSWEEVERVAEAFKEIAEKYGLEVED
EEEECCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475